##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935836.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	547482
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05429767554002	33.0	31.0	34.0	30.0	34.0
2	32.180773066511776	33.0	31.0	34.0	30.0	34.0
3	32.03570711000544	33.0	31.0	34.0	30.0	34.0
4	35.72584304141506	37.0	35.0	37.0	33.0	37.0
5	32.08559002853062	37.0	35.0	37.0	0.0	37.0
6	33.88707025984416	37.0	35.0	37.0	17.0	37.0
7	35.52762830558813	37.0	35.0	37.0	32.0	37.0
8	35.80294694620097	37.0	35.0	37.0	35.0	37.0
9	37.76210359427342	39.0	38.0	39.0	35.0	39.0
10	37.36318454305347	39.0	37.0	39.0	34.0	39.0
11	37.359262952937264	39.0	37.0	39.0	34.0	39.0
12	37.43583168031095	39.0	37.0	39.0	35.0	39.0
13	37.47630607033656	39.0	37.0	39.0	35.0	39.0
14	38.768416130575986	40.0	38.0	41.0	35.0	41.0
15	38.869438629945826	40.0	38.0	41.0	35.0	41.0
16	38.78966614427506	40.0	38.0	41.0	35.0	41.0
17	38.7603884693926	40.0	38.0	41.0	35.0	41.0
18	38.56824881913926	40.0	38.0	41.0	35.0	41.0
19	38.361631980594794	40.0	37.0	41.0	34.0	41.0
20	38.33223010071564	40.0	37.0	41.0	34.0	41.0
21	38.254552661092056	40.0	37.0	41.0	34.0	41.0
22	38.32099137505891	40.0	37.0	41.0	34.0	41.0
23	38.33657179596772	40.0	37.0	41.0	35.0	41.0
24	38.315487997778924	40.0	37.0	41.0	34.0	41.0
25	38.04687642698755	40.0	36.0	41.0	34.0	41.0
26	38.11927150116351	40.0	37.0	41.0	34.0	41.0
27	38.090625810528934	40.0	37.0	41.0	34.0	41.0
28	37.950657738519254	40.0	36.0	41.0	34.0	41.0
29	37.84437844531875	40.0	36.0	41.0	33.0	41.0
30	37.662029071275406	40.0	36.0	41.0	33.0	41.0
31	37.57557326085607	40.0	36.0	41.0	33.0	41.0
32	37.37087977321629	40.0	36.0	41.0	33.0	41.0
33	37.09744795262675	40.0	36.0	41.0	31.0	41.0
34	36.84087696033842	40.0	36.0	41.0	31.0	41.0
35	36.64613996441892	40.0	36.0	41.0	30.0	41.0
36	36.50139730621281	40.0	35.0	41.0	30.0	41.0
37	36.45271442714098	40.0	35.0	41.0	30.0	41.0
38	36.32019500184481	40.0	35.0	41.0	29.0	41.0
39	36.252749862095925	40.0	35.0	41.0	28.0	41.0
40	36.18532481433179	40.0	35.0	41.0	28.0	41.0
41	36.015781340756405	39.0	35.0	41.0	27.0	41.0
42	35.99364545318385	39.0	35.0	41.0	27.0	41.0
43	35.952730866037605	39.0	35.0	41.0	26.0	41.0
44	35.94467398014912	39.0	35.0	41.0	26.0	41.0
45	35.94791792241571	39.0	35.0	41.0	26.0	41.0
46	35.811876920154454	39.0	35.0	41.0	26.0	41.0
47	35.72246028179922	39.0	35.0	41.0	26.0	41.0
48	35.65996690302147	39.0	35.0	41.0	26.0	41.0
49	35.60542995020841	39.0	35.0	41.0	25.0	41.0
50	35.50720206326418	39.0	35.0	41.0	24.0	41.0
51	34.54447817462492	38.0	34.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	18.0
10	19.0
11	13.0
12	16.0
13	13.0
14	9.0
15	17.0
16	24.0
17	61.0
18	149.0
19	253.0
20	581.0
21	974.0
22	1503.0
23	2352.0
24	3800.0
25	6141.0
26	8487.0
27	9059.0
28	8412.0
29	8023.0
30	8253.0
31	9710.0
32	12042.0
33	15908.0
34	24768.0
35	33433.0
36	39126.0
37	60730.0
38	106436.0
39	187013.0
40	134.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.00222838376422	20.249432858066566	22.584304141506024	17.164034616663194
2	37.08889059366335	24.459616937177845	23.42341848681783	15.028073982340972
3	29.208266207838797	23.687719413606292	31.707526457490843	15.396487921064072
4	25.28978121655141	23.855578813550036	33.01606262854304	17.838577341355517
5	21.669753526143325	35.150562027610036	26.902071666283096	16.277612779963544
6	23.085690488454418	32.85989311064108	29.755133502106005	14.299282898798499
7	76.49438703007587	4.401971206359296	15.036658739465409	4.0669830240994225
8	78.01096657059044	4.485444270313909	13.630037151906363	3.8735520071892773
9	71.61897560102433	6.584326060034851	15.392652178519112	6.4040461604217125
10	30.826036289777566	33.835998261130044	22.42338560902459	12.914579840067802
11	22.271234488074494	24.87351182321976	34.79931760313581	18.055936085569936
12	20.32578240015197	22.064104390646634	38.0701100675456	19.5400031416558
13	21.32691120438663	23.080758819468038	38.720177101712935	16.872152874432402
14	18.594583931526515	27.48199940819972	35.50710342988445	18.41631323038931
15	17.66176787547353	26.734577575153157	38.054036479738144	17.549618069635166
16	20.980415794491876	27.099703734552005	34.087148070621495	17.83273240033462
17	21.432668105983392	24.965204335485	33.26228076904811	20.33984678948349
18	21.476687818046987	24.5776116840371	34.323137564340016	19.6225629335759
19	20.364505134415378	26.848188616246745	32.80637536941854	19.980930879919338
20	21.421526187162318	26.243602529398224	33.231777483095335	19.10309380034412
21	21.300608969792613	26.877413321351206	34.51163691226378	17.3103407965924
22	20.385327736802306	24.072389594543747	34.276743344986684	21.265539323667262
23	18.478233074329385	26.362145239478192	34.31345688077416	20.846164805418262
24	18.898520864612898	25.761212240767733	34.53483402194045	20.80543287267892
25	19.51589275994462	28.389243847286306	32.001600052604466	20.093263340164608
26	18.78308327945028	27.6264790440599	32.458053415454756	21.132384261035067
27	20.09709908270957	26.672292422399273	32.42389704136392	20.80671145352724
28	19.378901954767464	26.563065087071358	33.53260929126437	20.52542366689681
29	19.489042562129896	26.279585447558095	33.048575112971754	21.18279687734026
30	20.82187176930018	27.2456446056674	32.05365655857179	19.878827066460634
31	21.98958139263026	27.297335802820914	30.446845741047195	20.26623706350163
32	21.252936169590964	27.564741854526726	30.614887795397838	20.567434180484472
33	21.55322001453929	26.181317376644348	30.87480501642063	21.39065759239573
34	22.970618212105602	25.997201734486246	30.698726168166257	20.33345388524189
35	20.93365626632474	26.75320832465725	31.420393729839518	20.892741679178496
36	21.714138547020724	27.074862735213213	31.827347748419125	19.383650969346938
37	20.988269933988697	27.14080097610515	31.89803500389054	19.972894086015614
38	21.52965759604882	26.56069057978162	30.863297788785747	21.046354035383814
39	22.94048023496663	25.200828520389713	30.35113483183009	21.50755641281357
40	22.99728575551342	24.941276608180726	31.897121731856025	20.164315904449825
41	20.614924326279223	26.458769420729816	31.95009150985786	20.976214743133106
42	21.29604260962004	26.790469823665436	31.935113848491824	19.9783737182227
43	21.325632623538308	26.49676153736561	31.028234718219043	21.149371120877035
44	21.472852075502026	25.92815836867696	29.77705203093435	22.821937524886664
45	21.212934854479233	24.945842968353297	29.70526884902152	24.13595332814595
46	22.650425036804865	26.272096616875075	29.058124285364634	22.01935406095543
47	20.855845488984112	25.50640934313822	31.949360892230246	21.68838427564742
48	20.841963754059496	25.207404079038216	31.375460745741414	22.575171421160878
49	21.312846815055106	24.185452672416627	32.699339886973455	21.80236062555481
50	20.42551170632094	24.252121530936176	32.869573794206936	22.452792968535952
51	20.8996825466408	23.801878417920587	30.8727958179447	24.42564321749391
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	99.0
1	281.0
2	463.0
3	6171.5
4	11880.0
5	7876.5
6	3873.0
7	3777.5
8	3682.0
9	3700.0
10	3718.0
11	3713.5
12	3709.0
13	3627.5
14	3546.0
15	3445.5
16	3345.0
17	3218.5
18	3092.0
19	2949.0
20	2806.0
21	2851.5
22	2897.0
23	3120.0
24	3343.0
25	3647.0
26	5030.0
27	6109.0
28	7162.5
29	8216.0
30	9192.5
31	10169.0
32	11807.0
33	13445.0
34	14945.5
35	16446.0
36	18002.0
37	19558.0
38	21738.5
39	23919.0
40	25553.5
41	27188.0
42	29684.0
43	32180.0
44	36014.5
45	39849.0
46	48440.5
47	57032.0
48	56416.0
49	55800.0
50	53514.5
51	51229.0
52	44333.0
53	37437.0
54	32891.0
55	28345.0
56	24810.5
57	21276.0
58	18878.5
59	16481.0
60	14171.0
61	11861.0
62	10121.0
63	8381.0
64	6702.0
65	5023.0
66	4006.5
67	2990.0
68	2321.5
69	1653.0
70	1328.5
71	1004.0
72	777.0
73	550.0
74	480.0
75	335.0
76	260.0
77	206.5
78	153.0
79	104.5
80	56.0
81	49.0
82	42.0
83	24.0
84	6.0
85	6.5
86	7.0
87	4.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	547482.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.4147466608611
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.92596146313001	32.09476888582369
2	9.538306261770693	8.282062998569593
3	4.032857864040415	5.252565074597281
4	2.4548157809229334	4.263008209114123
5	1.807872795401117	3.9244169703701126
6	1.3669728953108524	3.5608069165310683
7	1.0818646091533455	3.2878214549420806
8	0.9024625205587151	3.134414536078302
9	0.7884255541865846	3.0806366126363627
>10	4.045488498344225	26.766372306832476
>50	0.03664783812533662	1.1128145925027884
>100	0.011931854268871061	0.9984805243595943
>500	0.0029829635672177653	1.0602727228283926
>1k	0.0029829635672177653	1.9355388634148742
>5k	4.2613765245968073E-4	1.2460193313992713
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6735	1.2301774304908655	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTC	3475	0.6347240639874918	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTT	1399	0.25553351525712265	No Hit
GCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC	1169	0.21352300166946128	TruSeq Adapter, Index 21 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGC	1165	0.21279238404184977	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCG	1147	0.20950460471759802	No Hit
CGCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTG	1102	0.20128515640696865	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTC	1005	0.18356767893738973	No Hit
GAACTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCT	997	0.18210644368216672	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTT	961	0.1755308850336632	No Hit
CCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC	823	0.1503245768810664	TruSeq Adapter, Index 21 (95% over 23bp)
CGTTTTCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCT	800	0.1461235255223003	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	767	0.14009593009450538	No Hit
CTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGCT	760	0.13881734924618527	TruSeq Adapter, Index 14 (95% over 22bp)
CGTCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCT	623	0.11379369550049134	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8265440690287536E-4	0.0	0.0	0.1466714887430089	0.0
2	1.8265440690287536E-4	0.0	0.0	0.6191984394007474	0.0
3	1.8265440690287536E-4	0.0	0.0	1.0067910908486488	0.0
4	1.8265440690287536E-4	0.0	0.0	1.5814218549650947	0.0
5	1.8265440690287536E-4	0.0	0.0	3.165218217219927	0.0
6	1.8265440690287536E-4	0.0	0.0	4.61567686243566	0.0
7	1.8265440690287536E-4	0.0	0.0	5.583197255800191	0.0
8	1.8265440690287536E-4	0.0	0.0	6.8407728473264875	0.0
9	1.8265440690287536E-4	0.0	0.0	7.474583639279465	0.0
10	1.8265440690287536E-4	0.0	0.0	8.653252527023719	0.0
11	1.8265440690287536E-4	0.0	0.0	9.744247299454594	0.0
12	3.653088138057507E-4	0.0	0.0	10.94008570144772	0.0
13	3.653088138057507E-4	0.0	0.0	11.528050237268074	0.0
14	3.653088138057507E-4	0.0	0.0	11.850069956637844	0.0
15	3.653088138057507E-4	0.0	0.0	12.175012146518059	0.0
16	3.653088138057507E-4	0.0	0.0	12.696307823818865	0.0
17	3.653088138057507E-4	0.0	0.0	13.333954358316802	0.0
18	3.653088138057507E-4	0.0	0.0	14.073339397459643	0.0
19	3.653088138057507E-4	0.0	0.0	14.560113391855806	0.0
20	3.653088138057507E-4	0.0	0.0	15.005059527071209	0.0
21	3.653088138057507E-4	0.0	0.0	15.499687660964197	0.0
22	3.653088138057507E-4	0.0	0.0	16.075779660335865	0.0
23	3.653088138057507E-4	0.0	0.0	16.653880858183467	0.0
24	3.653088138057507E-4	0.0	0.0	17.08731976576399	0.0
25	3.653088138057507E-4	0.0	0.0	17.50157996061971	0.0
26	3.653088138057507E-4	0.0	0.0	17.919858552427293	0.0
27	3.653088138057507E-4	0.0	0.0	18.318045159475563	0.0
28	3.653088138057507E-4	0.0	0.0	18.748926905359447	0.0
29	3.653088138057507E-4	0.0	0.0	19.183279084974483	0.0
30	3.653088138057507E-4	0.0	0.0	19.690693027350672	0.0
31	3.653088138057507E-4	0.0	0.0	20.173631279201874	0.0
32	3.653088138057507E-4	0.0	0.0	20.66880737631557	0.0
33	3.653088138057507E-4	0.0	0.0	21.176403973098658	0.0
34	3.653088138057507E-4	0.0	0.0	21.67833828326776	0.0
35	3.653088138057507E-4	0.0	0.0	22.15963264545684	0.0
36	3.653088138057507E-4	0.0	0.0	22.576997965229907	0.0
37	3.653088138057507E-4	0.0	0.0	23.025231879769564	0.0
38	3.653088138057507E-4	0.0	0.0	23.474561720750636	0.0
39	5.47963220708626E-4	0.0	0.0	23.98307158956824	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAAG	35	1.2103737E-7	45.000004	1
CGCACGG	35	1.2103737E-7	45.000004	2
TAGCACG	25	3.8881197E-5	45.0	1
ACTAGCG	20	7.0300454E-4	45.0	1
TCTACGG	20	7.0300454E-4	45.0	2
TATAGCG	20	7.0300454E-4	45.0	1
CGCCGGT	110	0.0	45.0	28
AATCGCG	20	7.0300454E-4	45.0	1
CGTTTTT	3685	0.0	44.32836	1
CGTTATT	535	0.0	44.15888	1
CGCCCAA	50	1.0786607E-9	40.5	37
TATTGCG	40	3.453806E-7	39.375	1
AAGGGCG	230	0.0	39.130432	5
ATAGCGG	75	0.0	39.000004	2
CGAACGG	35	6.242295E-6	38.57143	2
GGCCGGT	140	0.0	38.57143	9
CTTGCGG	70	0.0	38.57143	2
ACGCCGG	130	0.0	38.076927	27
CACGCCG	130	0.0	38.076927	26
ATGGGCG	160	0.0	37.96875	5
>>END_MODULE