##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935833.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	451582
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.11020146950056	33.0	31.0	34.0	30.0	34.0
2	32.21965888808677	33.0	31.0	34.0	30.0	34.0
3	32.17381560823948	34.0	31.0	34.0	30.0	34.0
4	35.79040351475479	37.0	35.0	37.0	35.0	37.0
5	32.0192191008499	37.0	35.0	37.0	0.0	37.0
6	33.84244500445102	37.0	35.0	37.0	17.0	37.0
7	35.51991221970761	37.0	35.0	37.0	32.0	37.0
8	35.76699691307448	37.0	35.0	37.0	35.0	37.0
9	37.70634569136945	39.0	37.0	39.0	35.0	39.0
10	37.355634192682615	39.0	37.0	39.0	34.0	39.0
11	37.32784743413156	39.0	37.0	39.0	34.0	39.0
12	37.18377393253053	39.0	37.0	39.0	34.0	39.0
13	37.09584748727806	39.0	37.0	39.0	33.0	39.0
14	38.18023304737567	40.0	37.0	41.0	33.0	41.0
15	38.32336319871031	40.0	38.0	41.0	34.0	41.0
16	38.33883990061606	40.0	37.0	41.0	34.0	41.0
17	38.325869941671726	40.0	37.0	41.0	34.0	41.0
18	38.26211850782361	40.0	37.0	41.0	34.0	41.0
19	38.16081686161096	40.0	37.0	41.0	34.0	41.0
20	38.232356471249965	40.0	37.0	41.0	34.0	41.0
21	38.159904513465996	40.0	37.0	41.0	34.0	41.0
22	38.25924638271676	40.0	37.0	41.0	34.0	41.0
23	38.27173137990442	40.0	37.0	41.0	34.0	41.0
24	38.27198382575036	40.0	37.0	41.0	34.0	41.0
25	38.01707995447117	40.0	36.0	41.0	34.0	41.0
26	38.070281809283806	40.0	36.0	41.0	34.0	41.0
27	38.05304684420548	40.0	36.0	41.0	34.0	41.0
28	37.970532483579944	40.0	36.0	41.0	34.0	41.0
29	37.94222311783906	40.0	36.0	41.0	34.0	41.0
30	37.78020160236679	40.0	36.0	41.0	33.0	41.0
31	37.78947787998636	40.0	36.0	41.0	33.0	41.0
32	37.72880008503439	40.0	36.0	41.0	33.0	41.0
33	37.67730334690045	40.0	36.0	41.0	33.0	41.0
34	37.55676488433994	40.0	36.0	41.0	33.0	41.0
35	37.533336138287176	40.0	36.0	41.0	33.0	41.0
36	37.46534184267752	40.0	35.0	41.0	33.0	41.0
37	37.43211421181535	40.0	35.0	41.0	33.0	41.0
38	37.32602052340439	40.0	35.0	41.0	33.0	41.0
39	37.258024013357485	40.0	35.0	41.0	33.0	41.0
40	37.170305725206056	40.0	35.0	41.0	32.0	41.0
41	37.02770703881023	39.0	35.0	41.0	32.0	41.0
42	37.02394692436811	39.0	35.0	41.0	32.0	41.0
43	36.98941056109411	39.0	35.0	41.0	32.0	41.0
44	36.962356781271176	39.0	35.0	41.0	32.0	41.0
45	36.93095827557343	39.0	35.0	41.0	32.0	41.0
46	36.82452577826397	39.0	35.0	41.0	32.0	41.0
47	36.73977040714643	39.0	35.0	41.0	32.0	41.0
48	36.69344437997972	39.0	35.0	41.0	32.0	41.0
49	36.66207023309166	39.0	35.0	41.0	32.0	41.0
50	36.56373371835016	39.0	35.0	41.0	31.0	41.0
51	35.51943611569992	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	9.0
9	26.0
10	21.0
11	13.0
12	15.0
13	8.0
14	7.0
15	23.0
16	37.0
17	53.0
18	90.0
19	186.0
20	307.0
21	597.0
22	993.0
23	1284.0
24	1806.0
25	2321.0
26	2810.0
27	3340.0
28	3971.0
29	4681.0
30	5881.0
31	7701.0
32	10629.0
33	16674.0
34	28707.0
35	34939.0
36	33620.0
37	53403.0
38	87919.0
39	149390.0
40	118.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.9838966123539	20.23065578344575	23.46816303572773	10.317284568472614
2	33.77614696777108	24.325150249567077	23.968847296836454	17.92985548582539
3	32.89524383168506	24.873002023995642	25.044620910488018	17.187133233831286
4	28.82045785704479	25.509209844502216	25.90426544902144	19.766066849431553
5	23.683184892223338	37.036020036228194	21.047783126873966	18.233011944674498
6	25.946339756677634	35.151533940679656	23.78637766784327	15.11574863479944
7	80.1338405870916	5.969458481516092	9.38544937575014	4.511251555642164
8	80.63142463605723	6.367614298178403	8.169944771935109	4.831016293829249
9	74.27466108037964	7.711777705931591	10.233135953160224	7.780425260528542
10	40.11918101252929	28.365833890633375	17.050281012086398	14.464704084750943
11	32.75927738483819	24.969551487880384	23.586635428338596	18.684535698942828
12	29.169896054315718	22.040736787560178	28.054484013977525	20.73488314414658
13	27.376201885814762	23.523745410578808	29.771337210074805	19.32871549353163
14	21.987368849954162	26.621078785248304	30.46489895522851	20.926653409569028
15	20.613753426841637	25.319654016324833	33.91787095145511	20.148721605378427
16	25.062114964724014	24.857279519555693	28.87891900031445	21.20168651540584
17	24.749214981996627	24.89116040940516	27.77590780854861	22.5837168000496
18	25.77449942646075	23.749618009575226	29.969086456058925	20.5067961079051
19	25.387415796023756	26.16313316296929	26.732243534950463	21.717207506056486
20	26.473818708451617	26.798012321128834	27.345199764383878	19.382969206035668
21	24.704926237095368	27.060866022117796	28.799642146941196	19.434565593845633
22	23.324667502247653	23.901306960862037	29.42522066867147	23.34880486821884
23	22.251551213290167	26.219822756442905	29.62784167659473	21.9007843536722
24	23.213945639994506	25.06255785217303	28.643745764888767	23.0797507429437
25	23.03236178589935	26.842743953479104	26.997311673184495	23.127582587437054
26	21.620436598447238	26.140103015620642	28.23518209317466	24.004278292757462
27	22.87712973502044	26.092492614851786	28.30161521052655	22.728762439601226
28	20.112404834559396	26.72582166693978	29.28238946636491	23.879384032135913
29	22.503554171778326	26.270090481905832	28.853010084547215	22.373345261768627
30	24.01690058505432	25.365492867297633	28.18668591750778	22.430920630140264
31	24.569624121422024	25.53290432302439	26.164904712765345	23.73256684278824
32	25.310353379895567	27.390152840458654	25.745047411101417	21.55444636854436
33	24.845543002156862	24.948735777776793	26.69880553255001	23.50691568751633
34	22.798074325371694	24.62299205902804	29.612340615879283	22.96659299972098
35	22.26638794283209	27.64459167991638	27.888844108046822	22.200176269204707
36	23.69979317156131	27.09696134921232	27.411411438011257	21.791834041215104
37	22.87425096660186	27.574836906696902	26.90098365302426	22.649928473676983
38	21.115987794021905	28.592592264527816	25.744604523652402	24.546815417797873
39	23.11828195100779	25.523160799146112	27.118662834213943	24.239894415632154
40	24.616791634741865	24.605940892241055	27.789194432018995	22.988073040998092
41	21.78031896754078	25.235505401012443	28.046954927344313	24.937220704102465
42	22.13573614537338	25.70142299737368	28.86341793959901	23.299422917653935
43	21.989140399750212	24.365895894876235	28.912799890163914	24.73216381520964
44	22.337028490949596	24.48392540003809	28.127560443064603	25.051485665947716
45	22.483845680297264	24.467981451873637	26.753280688778563	26.29489217905054
46	23.09503035993463	26.136781359753048	26.722057123623173	24.046131156689153
47	20.931525171508163	25.95896204897449	29.875858648041774	23.233654131475568
48	21.281406256228106	26.15427541398904	28.598571245089488	23.96574708469337
49	22.46989472565337	24.492118817844823	29.989237834988998	23.048748621512814
50	21.520344034970392	25.737961211917217	28.30803707853723	24.43365767457516
51	21.65719625671528	24.445615635698502	27.132170901408827	26.765017206177394
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	128.0
1	404.0
2	680.0
3	1206.0
4	1732.0
5	1180.5
6	629.0
7	638.5
8	648.0
9	653.0
10	658.0
11	665.0
12	672.0
13	670.5
14	669.0
15	711.5
16	754.0
17	767.5
18	781.0
19	920.0
20	1059.0
21	1150.0
22	1241.0
23	1462.5
24	1684.0
25	1968.5
26	3027.5
27	3802.0
28	4398.0
29	4994.0
30	5856.5
31	6719.0
32	7747.0
33	8775.0
34	10144.5
35	11514.0
36	12815.5
37	14117.0
38	14604.5
39	15092.0
40	17495.5
41	19899.0
42	22246.5
43	24594.0
44	27135.5
45	29677.0
46	34698.0
47	39719.0
48	41197.5
49	42676.0
50	43043.0
51	43410.0
52	38864.5
53	34319.0
54	31130.5
55	27942.0
56	26766.5
57	25591.0
58	24148.5
59	22706.0
60	20383.5
61	18061.0
62	16236.5
63	14412.0
64	12209.5
65	10007.0
66	8555.5
67	7104.0
68	6042.5
69	4981.0
70	4106.0
71	3231.0
72	2641.0
73	2051.0
74	1662.0
75	1026.0
76	779.0
77	568.0
78	357.0
79	241.0
80	125.0
81	83.0
82	41.0
83	28.5
84	16.0
85	9.5
86	3.0
87	3.5
88	4.0
89	3.0
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	451582.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.73915324404931
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.44121634356068	24.818073625002352
2	10.093808725761956	7.013007362807334
3	3.9701624213878732	4.137602422810776
4	2.2586793774561764	3.1385843609049604
5	1.449463307628171	2.5176563982660816
6	1.1166992094313326	2.3275910978366263
7	0.8925368731598622	2.170418264886554
8	0.7789158969838389	2.1647102967638165
9	0.6937403566065631	2.1689957303763134
>10	7.219268297505191	45.225613658949
>50	0.05400580453793325	1.2458303096745231
>100	0.027002902268966626	1.5020094715957635
>500	0.001928778733497616	0.44267401824577
>1k	0.0025717049779968213	1.1272329818801217
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGC	1352	0.2993919155325057	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTC	1280	0.2834479673680528	No Hit
GCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGC	1209	0.26772546292810606	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCG	1206	0.26706113175458723	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	829	0.18357684761571544	No Hit
CCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGC	624	0.13818088409192572	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	529	0.11714373026382806	No Hit
CTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGCT	482	0.10673587521203237	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.643311735188736E-4	0.0	0.0	0.11116474970215819	0.0
2	6.643311735188736E-4	0.0	0.0	0.640193807547688	0.0
3	6.643311735188736E-4	0.0	0.0	0.8713810559322559	0.0
4	6.643311735188736E-4	0.0	0.0	1.2290126710099163	0.0
5	6.643311735188736E-4	0.0	0.0	2.365683308900709	0.0
6	6.643311735188736E-4	0.0	0.0	3.036879237879278	0.0
7	6.643311735188736E-4	0.0	0.0	3.666000859201651	0.0
8	6.643311735188736E-4	0.0	0.0	4.558197625237498	0.0
9	6.643311735188736E-4	0.0	0.0	4.895013530211568	0.0
10	6.643311735188736E-4	0.0	0.0	5.95462175197417	0.0
11	6.643311735188736E-4	0.0	0.0	7.062283261954639	0.0
12	6.643311735188736E-4	0.0	0.0	8.2682657856159	0.0
13	6.643311735188736E-4	0.0	0.0	8.662878502686112	0.0
14	6.643311735188736E-4	0.0	0.0	8.832504395657931	0.0
15	6.643311735188736E-4	0.0	0.0	9.17529928119367	0.0
16	6.643311735188736E-4	0.0	0.0	9.80530667741407	0.0
17	6.643311735188736E-4	0.0	0.0	10.478717043637701	0.0
18	6.643311735188736E-4	0.0	0.0	11.204388128844817	0.0
19	6.643311735188736E-4	0.0	0.0	11.716587463627869	0.0
20	6.643311735188736E-4	0.0	0.0	12.221922042951224	0.0
21	6.643311735188736E-4	0.0	0.0	12.792582521003938	0.0
22	6.643311735188736E-4	0.0	0.0	13.414175055693097	0.0
23	6.643311735188736E-4	0.0	0.0	13.985721308643834	0.0
24	6.643311735188736E-4	0.0	0.0	14.460939541434335	0.0
25	6.643311735188736E-4	0.0	0.0	14.891647585599072	0.0
26	6.643311735188736E-4	0.0	0.0	15.287367521291815	0.0
27	6.643311735188736E-4	0.0	0.0	15.749299130611938	0.0
28	6.643311735188736E-4	0.0	0.0	16.174471081664016	0.0
29	6.643311735188736E-4	0.0	0.0	16.62909504807543	0.0
30	6.643311735188736E-4	0.0	0.0	17.175396716432452	0.0
31	8.857748980251648E-4	0.0	0.0	17.612305184883365	0.0
32	0.0011072186225314562	0.0	0.0	18.042570341599088	0.0
33	0.0011072186225314562	0.0	0.0	18.51601702459354	0.0
34	0.0013286623470377473	0.0	0.0	18.96909088493341	0.0
35	0.0013286623470377473	0.0	0.0	19.48394754441054	0.0
36	0.0013286623470377473	0.0	0.0	19.923070450106515	0.0
37	0.0013286623470377473	0.0	0.0	20.369058111262184	0.0
38	0.0013286623470377473	0.0	0.0	20.88059311487172	0.0
39	0.0013286623470377473	0.0	0.0	21.579248065689068	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGGT	35	1.2097371E-7	45.0	4
CGAAAGG	40	6.7993824E-9	45.0	2
CGGCCTA	20	7.028732E-4	45.0	44
TATGACG	20	7.028732E-4	45.0	1
CGTCCTA	20	7.028732E-4	45.0	25
CGTAAGG	105	0.0	42.857143	2
AACGGGC	95	0.0	42.63158	4
TAAGGGC	185	0.0	42.567566	4
GCGAGAC	70	0.0	41.785713	21
GTAACGG	60	3.6379788E-12	41.249996	2
TACGGGA	175	0.0	41.142857	4
TAACGGG	140	0.0	40.17857	3
CGGACGG	45	1.9237632E-8	40.0	2
CGGGTAT	70	0.0	38.571426	6
ATTAGCG	35	6.2398667E-6	38.571426	1
AACGGGA	205	0.0	38.414635	4
TAATACG	30	1.1388245E-4	37.499996	4
CTCGTAC	30	1.1388245E-4	37.499996	29
GGCCGAT	60	1.546141E-10	37.499996	8
GAATCGG	30	1.1388245E-4	37.499996	2
>>END_MODULE