##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935832.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	507024
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.95713417905267	33.0	31.0	34.0	30.0	34.0
2	32.08062537473571	33.0	31.0	34.0	30.0	34.0
3	31.96338043169554	33.0	31.0	34.0	30.0	34.0
4	35.671260137587176	37.0	35.0	37.0	33.0	37.0
5	32.020472403673196	37.0	35.0	37.0	0.0	37.0
6	33.82619165956641	37.0	35.0	37.0	17.0	37.0
7	35.46386561582884	37.0	35.0	37.0	32.0	37.0
8	35.70934511975764	37.0	35.0	37.0	33.0	37.0
9	37.665422149641834	39.0	37.0	39.0	35.0	39.0
10	37.188852993152196	39.0	37.0	39.0	34.0	39.0
11	37.24113454195462	39.0	37.0	39.0	34.0	39.0
12	37.26241164126353	39.0	37.0	39.0	34.0	39.0
13	37.25964648616239	39.0	37.0	39.0	34.0	39.0
14	38.51779205718073	40.0	38.0	41.0	34.0	41.0
15	38.59326580201332	40.0	38.0	41.0	34.0	41.0
16	38.56500481239547	40.0	38.0	41.0	34.0	41.0
17	38.52984474107735	40.0	38.0	41.0	34.0	41.0
18	38.38177482722711	40.0	38.0	41.0	34.0	41.0
19	38.17211216826028	40.0	37.0	41.0	34.0	41.0
20	38.110458282053706	40.0	37.0	41.0	34.0	41.0
21	38.11137539840323	40.0	37.0	41.0	34.0	41.0
22	38.180492442172365	40.0	37.0	41.0	34.0	41.0
23	38.169613272744485	40.0	37.0	41.0	34.0	41.0
24	38.14647038404494	40.0	36.0	41.0	34.0	41.0
25	37.93721993436208	40.0	36.0	41.0	34.0	41.0
26	37.95890727066175	40.0	36.0	41.0	34.0	41.0
27	37.91692306478589	40.0	36.0	41.0	34.0	41.0
28	37.83608073779545	40.0	36.0	41.0	33.0	41.0
29	37.792595616775536	40.0	36.0	41.0	33.0	41.0
30	37.596232131023385	40.0	36.0	41.0	33.0	41.0
31	37.51755538199375	40.0	36.0	41.0	33.0	41.0
32	37.30594015273439	40.0	36.0	41.0	33.0	41.0
33	37.04865252926883	40.0	36.0	41.0	31.0	41.0
34	36.78710672473098	40.0	36.0	41.0	31.0	41.0
35	36.69502627094575	40.0	36.0	41.0	30.0	41.0
36	36.547540155890054	40.0	36.0	41.0	30.0	41.0
37	36.46769383697813	40.0	35.0	41.0	30.0	41.0
38	36.3678602985263	40.0	35.0	41.0	29.0	41.0
39	36.29705299946354	40.0	35.0	41.0	29.0	41.0
40	36.233681245858186	40.0	35.0	41.0	29.0	41.0
41	36.10627307583073	39.0	35.0	41.0	27.0	41.0
42	36.106501861844805	40.0	35.0	41.0	28.0	41.0
43	36.02460830256556	39.0	35.0	41.0	27.0	41.0
44	36.01313152829057	39.0	35.0	41.0	27.0	41.0
45	36.051530105083785	39.0	35.0	41.0	27.0	41.0
46	35.92499171636845	39.0	35.0	41.0	27.0	41.0
47	35.7902051973871	39.0	35.0	41.0	26.0	41.0
48	35.74987180094039	39.0	35.0	41.0	26.0	41.0
49	35.73842461106378	39.0	35.0	41.0	26.0	41.0
50	35.618789248635174	39.0	35.0	41.0	26.0	41.0
51	34.549165325507275	38.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	17.0
10	12.0
11	16.0
12	6.0
13	8.0
14	9.0
15	8.0
16	30.0
17	58.0
18	127.0
19	292.0
20	569.0
21	1042.0
22	1698.0
23	2432.0
24	3823.0
25	5717.0
26	7661.0
27	7932.0
28	7144.0
29	6902.0
30	7410.0
31	9159.0
32	11772.0
33	16350.0
34	25450.0
35	34058.0
36	36180.0
37	55852.0
38	95239.0
39	169918.0
40	125.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.10782132601218	19.373244659029947	22.57723500268232	14.941699012275553
2	36.374215027296536	25.70489759853577	22.647251412161946	15.273635962005743
3	28.77634983748304	25.57018997128341	30.26010603048376	15.393354160749787
4	25.858144782101043	23.02652339928682	33.69958818517467	17.41574363343747
5	22.975243775442582	34.74332575972735	26.28337120136325	15.998059263466818
6	22.13958313610401	34.92162106724731	29.11144245637287	13.827353340275806
7	73.73082930985515	4.781036006185111	16.905708605509798	4.582426078449935
8	74.57714033260753	6.79297232478147	13.714932626463442	4.914954716147559
9	69.2428760768721	6.028314241534917	15.233203950897789	9.495605730695193
10	35.274464325160146	23.54247530688883	25.266851273312508	15.916209094638518
11	26.956120420335132	24.155661270472404	29.21380447473887	19.674413834453595
12	22.088895200227206	21.702917416138092	34.86541071034112	21.342776673293574
13	22.627922938559124	23.33577897693206	37.23176812143015	16.80452996307867
14	19.87953232983054	28.16217772728707	33.38106282937297	18.57722711350942
15	18.340946385180978	25.53961942629935	37.97828110700874	18.141153081510936
16	20.346768594780524	25.336275994824703	32.68109596389914	21.63585944649563
17	20.707106566947523	25.80134273722743	31.860819211713842	21.630731484111205
18	21.280846666035533	24.92110827100887	33.34654769793935	20.45149736501625
19	20.047374483259176	27.490217425605103	31.1139117674903	21.34849632364543
20	20.924256050995613	26.88886522137019	33.36725677679952	18.819621950834676
21	20.528219571460127	28.69233014610748	32.593526144718986	18.185924137713403
22	19.11467701726151	24.43986872416296	33.15503802581337	23.290416232762155
23	18.666177537946922	27.010555713339013	32.380912935087885	21.942353813626177
24	20.160978573006403	25.454219129666445	32.491361355675465	21.893440941651676
25	18.812127236580515	29.06391018965572	30.634447284546688	21.489515289217078
26	18.193813310612516	26.448057685632236	33.74002019628262	21.618108807472623
27	21.091309287134337	26.49874562150904	30.992615734166428	21.417329357190194
28	18.888849443024395	26.062277130865603	33.69603805737007	21.352835368739942
29	19.41486004607277	24.071838808419326	34.2455189497933	22.2677821957146
30	19.8505396194263	27.60579380857711	32.614037994256684	19.929628577739912
31	22.097376061093755	25.945122913313767	29.726009025213795	22.23149200037868
32	21.363091293508788	26.635622771308654	30.100744737921676	21.90054119726088
33	21.130755151629906	26.02164789043517	30.48514468743097	22.36245227050396
34	22.727720975732904	24.731965350752628	30.984529331944838	21.55578434156963
35	22.152008583420113	26.516299031209567	29.5258212628988	21.805871122471522
36	22.75059957714033	28.06021016756603	29.673348796112215	19.51584145918142
37	21.204913376881567	27.841680078260595	29.95873962573764	20.9946669191202
38	21.84216131780744	26.73482912051501	28.600421281832812	22.82258827984474
39	22.864795354854998	25.492481618227146	29.339834011802203	22.302889015115653
40	22.917652813279055	24.219760800277697	31.384313168607402	21.47827321783584
41	21.525805484552997	27.2517671747294	28.688188330335446	22.53423901038215
42	22.343321026223613	26.192842942345923	30.394024740446213	21.06981129098425
43	21.295047177253938	26.838374514815865	29.68261857426867	22.183959733661524
44	20.85068951371138	25.147921991858375	30.164055350437057	23.837333143993185
45	20.941217772728706	24.060202278393135	29.542191296664456	25.4563886522137
46	23.66041844173057	25.273359840954274	29.119923317239422	21.946298400075737
47	20.300025245353275	25.14141342421661	33.05681782321941	21.501743507210705
48	21.147322414718044	25.153049954242796	30.042759317113195	23.65686831392597
49	21.34297390261605	23.133421692069803	32.6521032534949	22.871501151819242
50	20.802171100381837	23.235980939758274	32.214648616239074	23.747199343620814
51	20.828797058916344	22.789650982990945	29.784191675344758	26.597360282747957
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	166.0
1	249.5
2	333.0
3	4213.0
4	8093.0
5	5596.0
6	3099.0
7	3088.0
8	3077.0
9	3023.0
10	2969.0
11	2975.0
12	2981.0
13	2929.5
14	2878.0
15	2773.0
16	2668.0
17	2506.5
18	2345.0
19	2339.0
20	2333.0
21	2298.5
22	2264.0
23	2312.5
24	2361.0
25	2777.5
26	3483.5
27	3773.0
28	4304.0
29	4835.0
30	6203.0
31	7571.0
32	8561.0
33	9551.0
34	11000.5
35	12450.0
36	14315.0
37	16180.0
38	17730.5
39	19281.0
40	21202.0
41	23123.0
42	26120.5
43	29118.0
44	33417.0
45	37716.0
46	50499.0
47	63282.0
48	60879.0
49	58476.0
50	56365.5
51	54255.0
52	46247.0
53	38239.0
54	32931.0
55	27623.0
56	23528.5
57	19434.0
58	17034.0
59	14634.0
60	12552.0
61	10470.0
62	9176.5
63	7883.0
64	6295.5
65	4708.0
66	3600.5
67	2493.0
68	2040.0
69	1587.0
70	1152.5
71	718.0
72	560.0
73	402.0
74	315.0
75	177.5
76	127.0
77	80.0
78	33.0
79	42.5
80	52.0
81	30.0
82	8.0
83	6.0
84	4.0
85	5.0
86	6.0
87	3.5
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	507024.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.25207804771357
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.76651947035498	35.50611049994182
2	9.661257627916498	8.93706483690924
3	3.7749688095811313	5.238004560252926
4	2.0297929562249175	3.7552856892805653
5	1.3726627243090601	3.174425172896489
6	1.0052569067486172	2.7897132545364096
7	0.7970453335432192	2.5805502082224803
8	0.6638468490777385	2.4563437020217833
9	0.552097903920653	2.29821078079054
>10	3.311181819822074	24.626090239595026
>50	0.039995872768625575	1.265286501898234
>100	0.017202525920305557	1.4916496583892322
>500	0.004300631480076389	1.3880159109534689
>1k	0.0038705683320687505	4.493248984311824
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTC	4552	0.8977878759190887	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4073	0.8033150304522073	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGC	3777	0.7449351509987693	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCG	3526	0.6954305910568336	No Hit
GCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCTGC	2010	0.3964309381804412	No Hit
CGCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCTG	1310	0.25837041244595915	No Hit
CCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCTGC	1292	0.2548202846413582	No Hit
GAACTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCT	1032	0.20354066079712202	No Hit
CTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCTGCT	1017	0.20058222095995457	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTT	955	0.188354002966329	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTC	900	0.17750639023004827	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCT	821	0.1619252737542996	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTT	763	0.1504859730505854	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCT	647	0.12760737164315694	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTAGCTGGAT	641	0.12642399570828994	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTC	621	0.12247940925873332	No Hit
CGTCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCT	595	0.11735144687430969	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	524	0.10334816497838367	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTAGCTGGATCGTA	511	0.10078418378617186	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.9445864495566286E-4	0.0	0.0	0.2064991006342895	0.0
2	3.9445864495566286E-4	0.0	0.0	1.0259869355296791	0.0
3	3.9445864495566286E-4	0.0	0.0	1.5377970273596515	0.0
4	3.9445864495566286E-4	0.0	0.0	2.2421029379279878	0.0
5	3.9445864495566286E-4	0.0	0.0	4.459552210546247	0.0
6	3.9445864495566286E-4	0.0	0.0	5.871516930165042	0.0
7	3.9445864495566286E-4	0.0	0.0	6.980537410457887	0.0
8	3.9445864495566286E-4	0.0	0.0	8.476127362807283	0.0
9	3.9445864495566286E-4	0.0	0.0	9.076296191107325	0.0
10	3.9445864495566286E-4	0.0	0.0	11.015652119031841	0.0
11	3.9445864495566286E-4	0.0	0.0	12.582639086118212	0.0
12	3.9445864495566286E-4	0.0	0.0	14.581361040108556	0.0
13	3.9445864495566286E-4	0.0	0.0	15.200858342011424	0.0
14	3.9445864495566286E-4	0.0	0.0	15.48762977689419	0.0
15	3.9445864495566286E-4	0.0	0.0	15.96058569219603	0.0
16	3.9445864495566286E-4	0.0	0.0	16.76823976774275	0.0
17	3.9445864495566286E-4	0.0	0.0	17.767009056770487	0.0
18	3.9445864495566286E-4	0.0	0.0	18.813902300482816	0.0
19	3.9445864495566286E-4	0.0	0.0	19.607947552778565	0.0
20	3.9445864495566286E-4	0.0	0.0	20.231192211808516	0.0
21	3.9445864495566286E-4	0.0	0.0	20.986186058253654	0.0
22	3.9445864495566286E-4	0.0	0.0	21.835258291520717	0.0
23	3.9445864495566286E-4	0.0	0.0	22.71983180283379	0.0
24	3.9445864495566286E-4	0.0	0.0	23.373252548202846	0.0
25	3.9445864495566286E-4	0.0	0.0	23.99294707942819	0.0
26	3.9445864495566286E-4	1.9722932247783143E-4	0.0	24.56708163716116	0.0
27	3.9445864495566286E-4	1.9722932247783143E-4	0.0	25.163305879011645	0.0
28	3.9445864495566286E-4	1.9722932247783143E-4	0.0	25.744343463031335	0.0
29	3.9445864495566286E-4	1.9722932247783143E-4	0.0	26.347076272523587	0.0
30	3.9445864495566286E-4	1.9722932247783143E-4	0.0	27.00897787875919	0.0
31	3.9445864495566286E-4	1.9722932247783143E-4	0.0	27.649775947489665	0.0
32	3.9445864495566286E-4	1.9722932247783143E-4	0.0	28.273809523809526	0.0
33	3.9445864495566286E-4	1.9722932247783143E-4	0.0	28.8852204234908	0.0
34	3.9445864495566286E-4	1.9722932247783143E-4	0.0	29.479669601438985	0.0
35	3.9445864495566286E-4	1.9722932247783143E-4	0.0	30.05419861781691	0.0
36	3.9445864495566286E-4	1.9722932247783143E-4	0.0	30.62892486351731	0.0
37	3.9445864495566286E-4	3.9445864495566286E-4	0.0	31.210554135504434	0.0
38	3.9445864495566286E-4	3.9445864495566286E-4	0.0	31.778969042885542	0.0
39	3.9445864495566286E-4	3.9445864495566286E-4	0.0	32.37203761557638	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGAA	70	0.0	45.0	31
AGTCCGC	20	7.0295535E-4	45.0	40
TTGGACG	20	7.0295535E-4	45.0	1
GTCGAAG	20	7.0295535E-4	45.0	1
CCGATCG	55	1.8189894E-12	45.0	40
GCGCGAG	25	3.8877104E-5	45.0	1
TCGATTG	20	7.0295535E-4	45.0	1
TAACGCG	20	7.0295535E-4	45.0	1
CCCCGAT	50	2.1827873E-11	45.0	38
AGGTTCG	20	7.0295535E-4	45.0	1
CTACGAA	20	7.0295535E-4	45.0	11
TACGACG	20	7.0295535E-4	45.0	1
TTGCGCG	20	7.0295535E-4	45.0	1
AACGAGC	25	3.8877104E-5	45.0	15
TATAGCG	20	7.0295535E-4	45.0	1
CGCACGG	20	7.0295535E-4	45.0	2
GCTACGA	20	7.0295535E-4	45.0	10
TAATGCG	20	7.0295535E-4	45.0	1
TCGAAAG	25	3.8877104E-5	45.0	1
CGCCCGG	25	3.8877104E-5	45.0	2
>>END_MODULE