##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935831.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	669686
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.99755109110837	33.0	31.0	34.0	30.0	34.0
2	32.16248809143389	33.0	31.0	34.0	30.0	34.0
3	31.95798030718874	33.0	31.0	34.0	30.0	34.0
4	35.69242002968556	37.0	35.0	37.0	33.0	37.0
5	32.24036787389911	37.0	35.0	37.0	10.0	37.0
6	33.931853137141886	37.0	35.0	37.0	19.0	37.0
7	35.49905179442306	37.0	35.0	37.0	32.0	37.0
8	35.748780771884135	37.0	35.0	37.0	35.0	37.0
9	37.674047240049816	39.0	37.0	39.0	35.0	39.0
10	37.39915572372724	39.0	37.0	39.0	34.0	39.0
11	37.34973256123019	39.0	37.0	39.0	34.0	39.0
12	37.233946954244225	39.0	37.0	39.0	34.0	39.0
13	37.18417437425898	39.0	37.0	39.0	34.0	39.0
14	38.30006450784398	40.0	38.0	41.0	33.0	41.0
15	38.46091750462157	40.0	38.0	41.0	34.0	41.0
16	38.42491854391461	40.0	38.0	41.0	34.0	41.0
17	38.43226228411524	40.0	38.0	41.0	34.0	41.0
18	38.18468655459424	40.0	37.0	41.0	34.0	41.0
19	37.89569589329925	39.0	37.0	41.0	34.0	41.0
20	37.78073156673426	39.0	35.0	41.0	34.0	41.0
21	37.66618385332828	39.0	35.0	41.0	33.0	41.0
22	37.72042569204075	39.0	35.0	41.0	34.0	41.0
23	37.74928100632236	39.0	35.0	41.0	34.0	41.0
24	37.69632036506661	39.0	35.0	41.0	34.0	41.0
25	37.41139728171113	39.0	35.0	41.0	33.0	41.0
26	37.4476590521528	39.0	35.0	41.0	33.0	41.0
27	37.41315034210063	39.0	35.0	41.0	33.0	41.0
28	37.26687880588813	39.0	35.0	41.0	33.0	41.0
29	37.171580412312636	39.0	35.0	41.0	33.0	41.0
30	36.903744441424784	39.0	35.0	41.0	32.0	41.0
31	36.70666700513375	39.0	35.0	41.0	31.0	41.0
32	36.40603656041787	39.0	35.0	41.0	31.0	41.0
33	36.037501157258774	39.0	35.0	41.0	29.0	41.0
34	35.643746472227285	39.0	35.0	41.0	26.0	41.0
35	35.42317444294789	39.0	35.0	41.0	24.0	41.0
36	35.25805078798123	39.0	35.0	41.0	23.0	41.0
37	35.160198958915075	39.0	35.0	41.0	23.0	41.0
38	35.01431566435613	38.0	35.0	41.0	23.0	41.0
39	34.88267635877113	38.0	35.0	41.0	22.0	41.0
40	34.79353159540441	38.0	35.0	41.0	21.0	41.0
41	34.61339493434237	38.0	34.0	40.0	21.0	41.0
42	34.60131165949415	38.0	34.0	40.0	21.0	41.0
43	34.53558832049647	38.0	34.0	40.0	20.0	41.0
44	34.482479848764946	38.0	34.0	40.0	20.0	41.0
45	34.4659064098697	38.0	34.0	40.0	20.0	41.0
46	34.35234572620601	38.0	34.0	40.0	20.0	41.0
47	34.29857276395206	37.0	34.0	40.0	20.0	41.0
48	34.24681268534806	37.0	34.0	40.0	20.0	41.0
49	34.19342348503627	37.0	34.0	40.0	20.0	41.0
50	34.09418891838862	37.0	34.0	40.0	20.0	41.0
51	33.118274833280076	35.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	8.0
8	11.0
9	31.0
10	29.0
11	19.0
12	15.0
13	18.0
14	26.0
15	41.0
16	66.0
17	174.0
18	299.0
19	492.0
20	963.0
21	1397.0
22	2253.0
23	3534.0
24	5843.0
25	9655.0
26	13652.0
27	15756.0
28	14947.0
29	13478.0
30	13367.0
31	14502.0
32	18168.0
33	25973.0
34	43340.0
35	50335.0
36	53129.0
37	74571.0
38	116245.0
39	177239.0
40	110.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.10099061351141	17.965135899511115	20.57143198454201	23.36244150243547
2	42.73301218780144	20.81587490256628	21.2271124079046	15.224000501727675
3	28.85621022389597	21.16290321135577	34.84229922680181	15.13858733794644
4	25.86884002353342	22.04032934838118	34.93069886484113	17.160131763244266
5	20.819906642814693	32.76445976173908	30.07902210886893	16.33661148657729
6	22.879976586041817	29.59073356767201	33.064749748389545	14.464540097896625
7	69.9468108934635	4.968298575750427	19.918140740585887	5.166749790200184
8	71.20725235408834	4.569753586008965	19.384607114378976	4.838386945523723
9	65.12484955635925	5.732238690968603	21.58115893120059	7.561752821471555
10	37.617779078553234	19.659661393548618	27.5240933810771	15.198466146821046
11	27.509758304638293	20.632505383119852	33.08296724136387	18.774769070877994
12	24.481772054365777	17.9821588027822	37.58447989057559	19.951589252276438
13	23.234321756763617	17.607356283392512	40.188088148774206	18.970233811069665
14	18.56481993053461	21.03732196880329	40.54571844117989	19.852139659482205
15	17.93273265381089	21.017909886125736	41.11792690902901	19.931430551034364
16	22.209513115101704	21.837697069970105	37.07035834704623	18.882431467881965
17	22.260731148627862	22.06795423526847	35.19739101608813	20.473923600015528
18	22.530111126707144	22.017632144019736	35.74511039502095	19.707146334252172
19	21.41720149443172	23.986166651236548	33.96845685888611	20.62817499544563
20	21.41585758101558	24.805057892803493	33.71893693462309	20.060147591557833
21	22.04062799580699	24.08934933685339	35.64133041455249	18.228692252787127
22	21.127662815110366	21.07420492589064	36.65434248289497	21.143789776104025
23	20.47914992996718	22.33180923597029	35.95296900338368	21.236071830678853
24	20.98237084245452	22.190996974701577	35.36493222196671	21.461699960877187
25	22.32688155344445	23.01556251735888	33.23617337080363	21.421382558393038
26	20.117039926174357	22.137688409194755	33.970248743440955	23.77502292118993
27	20.98237084245452	21.269818989795215	34.88993349121827	22.857876676531987
28	20.167511341135995	22.218024566737245	34.54245721129007	23.07200688083669
29	21.27773314657914	21.88936307463498	33.903501043772756	22.929402735013127
30	22.678389573621068	20.387913141382676	35.02447415654501	21.909223128451245
31	22.815020770928466	20.86858617322148	35.009541785254584	21.306851270595473
32	24.043805604417592	21.144835042094353	33.546766693644486	21.26459265984357
33	24.94676609634963	20.532458495474	32.88884641458833	21.63192899358804
34	22.82248695657368	21.701215196375614	34.364612669221096	21.11168517782961
35	22.4233446719806	22.944036458877743	33.11178671795438	21.52083215118727
36	23.518783429846227	24.2545013633255	31.01976150016575	21.206953706662528
37	22.729010312295614	25.010527321759753	31.29421848448377	20.966243881460862
38	22.67062474055005	24.196115791579935	31.198352660799237	21.934906807070774
39	23.096794617178798	23.630029595959897	30.195345281221346	23.077830505639955
40	24.18058612543789	22.49307884590689	31.718447152844764	21.607887875810455
41	21.02194162637415	24.249125709660944	31.337522361226007	23.391410302738898
42	21.057928641184077	24.77937421418396	31.68350540402517	22.47919174060679
43	21.142445862687886	23.717085320583077	32.36113641318469	22.77933240354435
44	22.618062793607752	22.633293812323984	31.24538963036408	23.503253763704183
45	22.184874702472502	22.025695624516565	30.887908661671293	24.901521011339643
46	22.520255761655463	22.54429687943305	31.00647168971727	23.92897566919422
47	21.381662450760505	22.738268382495676	32.71772143960005	23.162347727143768
48	20.66371403911684	22.858175323957795	33.216462640700264	23.261647996225097
49	21.849642967002445	21.96686208163229	33.10118473433818	23.082310217027086
50	20.73986913269801	21.715699596527326	34.201700498442555	23.342730772332107
51	20.359093664792155	21.581606902339306	33.216462640700264	24.842836792168267
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	235.0
1	569.0
2	903.0
3	14090.5
4	27278.0
5	17552.0
6	7826.0
7	7579.5
8	7333.0
9	7267.5
10	7202.0
11	6965.5
12	6729.0
13	6414.0
14	6099.0
15	5703.5
16	5308.0
17	4923.0
18	4538.0
19	4249.5
20	3961.0
21	3845.5
22	3730.0
23	3709.0
24	3688.0
25	3734.0
26	4069.5
27	4359.0
28	4907.0
29	5455.0
30	6322.5
31	7190.0
32	8197.0
33	9204.0
34	10448.0
35	11692.0
36	12868.0
37	14044.0
38	15181.5
39	16319.0
40	18040.0
41	19761.0
42	22110.5
43	24460.0
44	27963.0
45	31466.0
46	41809.5
47	52153.0
48	55357.5
49	58562.0
50	59474.0
51	60386.0
52	54286.5
53	48187.0
54	43843.0
55	39499.0
56	38036.0
57	36573.0
58	34818.0
59	33063.0
60	30497.0
61	27931.0
62	25411.0
63	22891.0
64	20591.5
65	18292.0
66	15818.5
67	13345.0
68	11540.0
69	9735.0
70	8133.0
71	6531.0
72	5534.5
73	4538.0
74	3658.0
75	2152.5
76	1527.0
77	1106.0
78	685.0
79	477.5
80	270.0
81	178.5
82	87.0
83	67.0
84	47.0
85	36.0
86	25.0
87	17.5
88	10.0
89	9.0
90	8.0
91	5.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	669686.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.63654936259083
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.34853073729059	30.8469170209141
2	9.28331955509432	7.916174249189674
3	4.263922033403462	5.453967667663361
4	2.734528875819536	4.663635015892386
5	2.1540598727964126	4.592084004823018
6	1.754308716557124	4.487860211442669
7	1.4273039855489753	4.259872178469705
8	1.2484956103558518	4.258523577593219
9	1.0580998286501682	4.060235301731261
>10	3.6615948362929056	21.059900876545047
>50	0.03858128331843368	1.1584230084332099
>100	0.020175531139168677	1.4928503631839418
>500	0.004247480239824677	1.2337294499624138
>1k	0.002477696806564395	2.1183926959171133
>5k	0.0	0.0
>10k+	3.5395668665205645E-4	2.3974343782388874
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15886	2.372156503197021	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTT	2989	0.4463285778708231	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTC	2289	0.3418019788378434	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTC	2070	0.3091000857118112	No Hit
CGCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTG	2028	0.3028284897698324	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1847	0.27580089773416194	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCT	1712	0.25564219649208736	No Hit
CGTCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCT	1102	0.1645547316204908	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	852	0.12722380339442665	No Hit
GCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC	826	0.12334138685891596	No Hit
CCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC	791	0.11811505690726698	No Hit
GAACTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCT	712	0.1063184835878307	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTC	706	0.10542254131040518	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCC	699	0.10437727532007539	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.4797113871276986E-4	0.0	0.0	0.10228674333941579	0.0
2	4.4797113871276986E-4	0.0	0.0	0.3900335381059183	0.0
3	4.4797113871276986E-4	0.0	0.0	0.8708558936576246	0.0
4	4.4797113871276986E-4	0.0	0.0	1.3681038576287992	0.0
5	4.4797113871276986E-4	0.0	0.0	2.7448684906060454	0.0
6	4.4797113871276986E-4	0.0	0.0	4.6206729721093165	0.0
7	4.4797113871276986E-4	0.0	0.0	5.894852214321339	0.0
8	4.4797113871276986E-4	0.0	0.0	7.397646060989778	0.0
9	4.4797113871276986E-4	0.0	0.0	8.47860041870369	0.0
10	4.4797113871276986E-4	0.0	0.0	9.767562708493234	0.0
11	4.4797113871276986E-4	0.0	0.0	10.935722114543234	0.0
12	5.972948516170265E-4	0.0	0.0	11.901846537033775	0.0
13	5.972948516170265E-4	0.0	0.0	12.441651759182662	0.0
14	5.972948516170265E-4	0.0	0.0	12.861699363582336	0.0
15	5.972948516170265E-4	0.0	0.0	13.147803597506892	0.0
16	5.972948516170265E-4	0.0	0.0	13.481094124709193	0.0
17	5.972948516170265E-4	0.0	0.0	13.82797310978578	0.0
18	7.466185645212831E-4	0.0	0.0	14.242495736807996	0.0
19	7.466185645212831E-4	0.0	0.0	14.539351278061659	0.0
20	7.466185645212831E-4	0.0	0.0	14.84322503382182	0.0
21	7.466185645212831E-4	0.0	0.0	15.152474443246536	0.0
22	7.466185645212831E-4	0.0	0.0	15.49950275203603	0.0
23	7.466185645212831E-4	0.0	0.0	15.831748013248	0.0
24	7.466185645212831E-4	0.0	0.0	16.12681166994681	0.0
25	7.466185645212831E-4	0.0	0.0	16.404404452235823	0.0
26	7.466185645212831E-4	0.0	0.0	16.693793807844273	0.0
27	7.466185645212831E-4	0.0	0.0	17.016183704004565	0.0
28	7.466185645212831E-4	0.0	0.0	17.301839966790407	0.0
29	7.466185645212831E-4	0.0	0.0	17.61810759072162	1.4932371290425663E-4
30	7.466185645212831E-4	0.0	0.0	18.071753030524754	1.4932371290425663E-4
31	8.959422774255397E-4	0.0	0.0	18.40355032059801	1.4932371290425663E-4
32	8.959422774255397E-4	0.0	0.0	18.787760233900663	1.4932371290425663E-4
33	8.959422774255397E-4	0.0	0.0	19.162712077003253	1.4932371290425663E-4
34	8.959422774255397E-4	0.0	0.0	19.53452812213485	1.4932371290425663E-4
35	8.959422774255397E-4	0.0	0.0	19.902013779592227	1.4932371290425663E-4
36	8.959422774255397E-4	0.0	0.0	20.22305976233638	1.4932371290425663E-4
37	8.959422774255397E-4	0.0	0.0	20.58621503211953	1.4932371290425663E-4
38	0.0010452659903297963	0.0	0.0	21.02955713573227	1.4932371290425663E-4
39	0.0010452659903297963	0.0	0.0	21.728989406975806	1.4932371290425663E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAGCG	30	2.164139E-6	45.000004	1
ACCGCGT	20	7.0311746E-4	45.0	23
CGCATCG	20	7.0311746E-4	45.0	21
TTAACGG	25	3.8890557E-5	45.0	2
ACGTCCC	20	7.0311746E-4	45.0	43
TACGTAG	20	7.0311746E-4	45.0	1
TCACGAC	65	0.0	44.999996	25
CGTTATT	795	0.0	44.71698	1
CGTTTTT	7195	0.0	43.092422	1
TACGGGA	180	0.0	42.5	4
TTACGGG	155	0.0	42.096775	3
CTCACGA	70	0.0	41.785713	24
CTTACGG	55	6.002665E-11	40.90909	2
CACAACG	150	0.0	40.500004	12
CGTTTCT	345	0.0	40.434784	1
GTTATTT	910	0.0	40.054943	2
CGTTAGG	90	0.0	40.0	2
CGGTCTA	75	0.0	39.000004	31
TAACGGG	145	0.0	38.793102	3
CATAGCG	35	6.244385E-6	38.571426	1
>>END_MODULE