SRR2935830.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935830.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	499177
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGC	1236	0.24760756204712958	RNA PCR Primer, Index 40 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTC	1124	0.22517063085839292	RNA PCR Primer, Index 46 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGC	961	0.1925168827890708	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCG	885	0.17729182233957094	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	814	0.16306841060385394	No Hit
CCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGC	746	0.1494459880964067	RNA PCR Primer, Index 46 (96% over 26bp)
CTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGCT	588	0.11779388874086746	RNA PCR Primer, Index 46 (96% over 27bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	35	1.2100827E-7	45.0	25
CTCACGA	45	3.8380676E-10	45.0	24
TTTACGG	20	7.029448E-4	45.0	2
ATTACGG	25	3.8876242E-5	45.0	2
CGTTCGA	20	7.029448E-4	45.0	18
TTGCGCA	20	7.029448E-4	45.0	1
TCGTAAG	20	7.029448E-4	45.0	1
CGGTCTA	35	1.2100827E-7	45.0	31
TAGGCGG	35	1.2100827E-7	45.0	2
TTTCTCG	20	7.029448E-4	45.0	12
GCGTATT	20	7.029448E-4	45.0	12
ATAGCGG	50	1.0786607E-9	40.5	2
CACGGGT	90	0.0	40.0	4
CGTAAGG	90	0.0	40.0	2
TGCGAAG	80	0.0	39.375	1
TTACGGG	155	0.0	39.193546	3
TTTAGCG	35	6.241189E-6	38.571426	1
GCGCGAC	35	6.241189E-6	38.571426	9
TAGCGCG	35	6.241189E-6	38.571426	1
ACGGGCG	70	0.0	38.571426	5