##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2935830.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 499177 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.03846932050155 33.0 31.0 34.0 30.0 34.0 2 32.142436450397355 33.0 31.0 34.0 30.0 34.0 3 32.11362102019925 33.0 31.0 34.0 30.0 34.0 4 35.72841897763719 37.0 35.0 37.0 33.0 37.0 5 31.999947914266883 37.0 35.0 37.0 0.0 37.0 6 33.809290091490595 37.0 35.0 37.0 17.0 37.0 7 35.49122455561855 37.0 35.0 37.0 32.0 37.0 8 35.742880781766786 37.0 35.0 37.0 35.0 37.0 9 37.68814067955855 39.0 37.0 39.0 35.0 39.0 10 37.21486166229614 39.0 37.0 39.0 34.0 39.0 11 37.212455702085634 39.0 37.0 39.0 34.0 39.0 12 37.146461074929334 39.0 37.0 39.0 34.0 39.0 13 37.11011524970101 39.0 37.0 39.0 33.0 39.0 14 38.31019057368428 40.0 38.0 41.0 34.0 41.0 15 38.4604799499977 40.0 38.0 41.0 34.0 41.0 16 38.386832726668096 40.0 38.0 41.0 34.0 41.0 17 38.38818695572913 40.0 38.0 41.0 34.0 41.0 18 38.33952485791613 40.0 37.0 41.0 34.0 41.0 19 38.21961949368661 40.0 37.0 41.0 34.0 41.0 20 38.29924054994521 40.0 37.0 41.0 34.0 41.0 21 38.24017532859086 40.0 37.0 41.0 34.0 41.0 22 38.295039635239604 40.0 37.0 41.0 34.0 41.0 23 38.32869102542785 40.0 37.0 41.0 34.0 41.0 24 38.30129593310589 40.0 37.0 41.0 34.0 41.0 25 38.04205321959946 40.0 37.0 41.0 34.0 41.0 26 38.09876456647642 40.0 37.0 41.0 34.0 41.0 27 38.09295901053134 40.0 37.0 41.0 34.0 41.0 28 38.029911233890985 40.0 37.0 41.0 34.0 41.0 29 38.01013267838863 40.0 37.0 41.0 34.0 41.0 30 37.85247116754177 40.0 36.0 41.0 33.0 41.0 31 37.87469975579804 40.0 37.0 41.0 33.0 41.0 32 37.824609306919186 40.0 36.0 41.0 33.0 41.0 33 37.758234053251655 40.0 36.0 41.0 33.0 41.0 34 37.71239460151409 40.0 36.0 41.0 33.0 41.0 35 37.71464230122782 40.0 36.0 41.0 33.0 41.0 36 37.64381972727109 40.0 36.0 41.0 33.0 41.0 37 37.625878195509806 40.0 36.0 41.0 33.0 41.0 38 37.5101016272785 40.0 36.0 41.0 33.0 41.0 39 37.39803716918047 40.0 36.0 41.0 33.0 41.0 40 37.341942837911205 40.0 35.0 41.0 33.0 41.0 41 37.256720562045125 40.0 35.0 41.0 32.0 41.0 42 37.2717112366956 40.0 35.0 41.0 33.0 41.0 43 37.19113661086148 40.0 35.0 41.0 32.0 41.0 44 37.1313642255152 39.0 35.0 41.0 32.0 41.0 45 37.106092628466456 39.0 35.0 41.0 32.0 41.0 46 36.966252451535226 39.0 35.0 41.0 32.0 41.0 47 36.89463256520232 39.0 35.0 41.0 32.0 41.0 48 36.86990586505388 39.0 35.0 41.0 32.0 41.0 49 36.81441452631031 39.0 35.0 41.0 32.0 41.0 50 36.69597557579776 39.0 35.0 41.0 31.0 41.0 51 35.669063278155846 38.0 34.0 40.0 29.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 14.0 9 24.0 10 28.0 11 28.0 12 15.0 13 15.0 14 21.0 15 25.0 16 40.0 17 55.0 18 101.0 19 170.0 20 292.0 21 556.0 22 860.0 23 1274.0 24 1806.0 25 2373.0 26 2990.0 27 3601.0 28 4082.0 29 5095.0 30 6503.0 31 8446.0 32 11611.0 33 17116.0 34 29254.0 35 38827.0 36 37643.0 37 59383.0 38 99392.0 39 167432.0 40 104.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.571765125396404 20.53700390843328 23.599044026467567 11.29218693970275 2 32.414554356470745 24.452849390096098 25.430458534748198 17.702137718684956 3 33.16038198875349 24.05299122355397 25.26037858314786 17.526248204544682 4 29.46529988360842 25.64861762461011 25.91185090659225 18.974231585189223 5 23.414740663131514 37.184004872019344 21.553877682665668 17.847376782183474 6 25.42785424809236 35.397664555858945 23.78134409237605 15.393137103672643 7 81.02697039326732 6.06077603735749 8.427471618283695 4.484781951091497 8 82.46794223291538 5.60662851052833 7.4168080660767615 4.50862119047953 9 76.57985043381406 7.407793227652716 9.344981840108819 6.667374498424407 10 40.8071685995148 25.65542883586383 18.528097248070324 15.009305316551044 11 30.656460534039027 26.246401576995737 23.27390885397364 19.823229034991595 12 26.76946253533316 22.929542026175085 28.913591772056808 21.387403666434952 13 25.335101577196067 23.833029166007247 30.335932945628503 20.495936311168183 14 22.107188432159333 27.25706512920267 29.24814244246029 21.38760399617771 15 21.823321186673265 25.27740661128217 32.19018504458339 20.70908715746118 16 23.35544305927557 25.488353830404847 29.181031978636835 21.97517113168275 17 22.902096851417433 25.146991948747637 28.214841629321864 23.736069570513067 18 23.828020922438334 24.68142562658135 29.76198823263091 21.728565218349402 19 23.819206413757044 26.783285287583364 27.20197444994461 22.195533848714984 20 24.62873890423637 27.07736934995002 27.956616590908634 20.337275154904972 21 24.323236046532593 27.47943114366247 27.845834243164248 20.351498566640693 22 23.195179265070305 24.696450357288096 28.36849454201616 23.73987583562544 23 22.14505075354033 26.2682375189562 27.94820274171286 23.63850898579061 24 22.33696664710113 25.68668027573386 28.407358512110935 23.56899456505408 25 21.35775486450698 27.231422922129827 27.729843322108188 23.680978891255005 26 20.221284233848916 27.412921669067288 27.60143195700123 24.76436214008258 27 21.684492674942955 26.70455569868003 27.383673526624825 24.22727809975219 28 20.192637080634725 26.256418064133562 29.1235373424657 24.427407512766013 29 20.97071780150127 24.812441278344156 28.749121053253656 25.467719866900918 30 21.85096669117367 26.095953940185545 28.1671631505458 23.885916218094984 31 23.350434815706652 26.51284013486198 25.66624664197269 24.470478407458675 32 23.560981375343815 26.90849137680622 25.871784958040934 23.658742289809027 33 22.547913866223805 26.644456775853055 26.13421692105205 24.67341243687109 34 21.443896653892306 26.388435364610146 27.107218481620748 25.060449499876796 35 20.877364141376706 26.357584584225634 27.491050268742352 25.27400100565531 36 21.434080496497234 27.796152466960617 26.5482984993299 24.22146853721225 37 21.523627891509424 27.11783595798685 27.157100587567136 24.201435562936595 38 21.578117581539214 27.730043651850945 26.363594476508332 24.32824429010151 39 22.114600632641327 27.373657039487 26.705757677136567 23.80598465073511 40 22.136436574601795 26.615809622638864 27.970439343158837 23.2773144596005 41 19.793980892549136 26.72699262986876 27.214394893995518 26.264631583586585 42 20.705481222091564 25.641806413356385 27.730243981593706 25.922468382958346 43 22.193129891801906 24.949466822389653 27.188752686922673 25.66865059888577 44 21.797077990372152 25.045424769170054 27.166516085476694 25.990981154981096 45 22.109792718815168 25.185254929614143 26.20914024484301 26.495812106727673 46 22.40207381349702 26.39805119226246 27.378464953313152 23.821410040927365 47 20.08806495491579 26.264230924101074 28.850487903088485 24.797216217894654 48 20.750355084469035 25.44688557365424 28.558807797634906 25.24395154424182 49 21.34994200453947 24.472481704886242 29.75958427571783 24.417992014856456 50 20.93866504266022 24.896579770301916 29.133553829603525 25.031201357434334 51 20.536202589462256 24.805029077862162 27.500065107166392 27.15870322550919 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 138.0 1 384.0 2 630.0 3 1131.5 4 1633.0 5 1117.5 6 602.0 7 604.0 8 606.0 9 635.5 10 665.0 11 656.5 12 648.0 13 655.0 14 662.0 15 728.0 16 794.0 17 843.0 18 892.0 19 916.5 20 941.0 21 910.5 22 880.0 23 1217.5 24 1555.0 25 1900.5 26 2982.0 27 3718.0 28 4246.0 29 4774.0 30 5983.5 31 7193.0 32 8419.0 33 9645.0 34 10945.0 35 12245.0 36 13338.0 37 14431.0 38 15982.0 39 17533.0 40 20004.5 41 22476.0 42 24952.5 43 27429.0 44 30888.5 45 34348.0 46 39735.5 47 45123.0 48 47114.0 49 49105.0 50 49210.5 51 49316.0 52 45057.0 53 40798.0 54 37384.5 55 33971.0 56 31394.0 57 28817.0 58 26167.5 59 23518.0 60 21363.0 61 19208.0 62 16829.0 63 14450.0 64 12186.5 65 9923.0 66 8472.5 67 7022.0 68 5793.0 69 4564.0 70 3677.0 71 2790.0 72 2348.0 73 1906.0 74 1409.5 75 787.5 76 662.0 77 432.5 78 203.0 79 158.0 80 113.0 81 69.5 82 26.0 83 29.0 84 32.0 85 21.5 86 11.0 87 12.5 88 14.0 89 8.5 90 3.0 91 2.5 92 2.0 93 2.5 94 3.0 95 1.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 499177.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.139615288305826 #Duplication Level Percentage of deduplicated Percentage of total 1 71.28789342030859 24.33741256083083 2 10.334208798380901 7.056118253714984 3 4.101968286176935 4.201188576447351 4 2.0900512975010224 2.8541418891803736 5 1.3866342537055345 2.3669579983547004 6 1.0575909916685768 2.1663449752765835 7 0.8562134446590159 2.0461558323733757 8 0.7416058524754443 2.02545107992542 9 0.6468707812659348 1.9875527652298355 >10 7.423968216919363 47.24862094072485 >50 0.043915484662317594 1.0816942075186031 >100 0.024925004808342414 1.3410252780606167 >500 0.0029672624771836208 0.8091939810506276 >1k 0.0011869049908734483 0.478141661311838 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGC 1236 0.24760756204712958 RNA PCR Primer, Index 40 (95% over 23bp) GAATCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTC 1124 0.22517063085839292 RNA PCR Primer, Index 46 (95% over 23bp) GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGC 961 0.1925168827890708 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCG 885 0.17729182233957094 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 814 0.16306841060385394 No Hit CCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGC 746 0.1494459880964067 RNA PCR Primer, Index 46 (96% over 26bp) CTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGCT 588 0.11779388874086746 RNA PCR Primer, Index 46 (96% over 27bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 6.00989228269732E-4 0.0 0.0 0.12580707845113057 0.0 2 6.00989228269732E-4 0.0 0.0 0.6638927674952972 0.0 3 6.00989228269732E-4 0.0 0.0 0.9159075838830716 0.0 4 6.00989228269732E-4 0.0 0.0 1.2895225541240882 0.0 5 6.00989228269732E-4 0.0 0.0 2.39914899925277 0.0 6 6.00989228269732E-4 0.0 0.0 3.0468150575847845 0.0 7 6.00989228269732E-4 0.0 0.0 3.665633632959852 0.0 8 6.00989228269732E-4 0.0 0.0 4.614595624397759 0.0 9 6.00989228269732E-4 0.0 0.0 4.939530467148927 0.0 10 6.00989228269732E-4 0.0 0.0 5.9011132323804985 0.0 11 6.00989228269732E-4 0.0 0.0 7.050404966574982 0.0 12 6.00989228269732E-4 0.0 0.0 8.223335610414743 0.0 13 6.00989228269732E-4 0.0 0.0 8.657650492711003 0.0 14 6.00989228269732E-4 0.0 0.0 8.844758472445646 0.0 15 6.00989228269732E-4 0.0 0.0 9.175903537222268 0.0 16 6.00989228269732E-4 0.0 0.0 9.839395645232052 0.0 17 6.00989228269732E-4 0.0 0.0 10.6068588897325 0.0 18 6.00989228269732E-4 0.0 0.0 11.461866231817572 0.0 19 6.00989228269732E-4 0.0 0.0 12.000352580347252 0.0 20 6.00989228269732E-4 0.0 0.0 12.492963417785676 0.0 21 6.00989228269732E-4 0.0 0.0 13.117591555700683 0.0 22 6.00989228269732E-4 0.0 0.0 13.777477728340848 0.0 23 8.013189710263094E-4 0.0 0.0 14.433958295354152 0.0 24 8.013189710263094E-4 0.0 0.0 14.980658163336852 0.0 25 8.013189710263094E-4 0.0 0.0 15.485489115083427 0.0 26 8.013189710263094E-4 0.0 0.0 15.935830376800213 0.0 27 8.013189710263094E-4 0.0 0.0 16.398191423082395 0.0 28 8.013189710263094E-4 0.0 0.0 16.824693445411146 0.0 29 8.013189710263094E-4 0.0 0.0 17.295869000374616 0.0 30 8.013189710263094E-4 0.0 0.0 17.848578760640013 0.0 31 8.013189710263094E-4 0.0 0.0 18.34499586319081 0.0 32 8.013189710263094E-4 0.0 0.0 18.818976034552875 0.0 33 8.013189710263094E-4 0.0 0.0 19.283340378262622 0.0 34 8.013189710263094E-4 0.0 0.0 19.756519230653655 0.0 35 8.013189710263094E-4 0.0 0.0 20.26435512854158 0.0 36 0.0010016487137828867 0.0 0.0 20.711891773859772 0.0 37 0.0010016487137828867 0.0 0.0 21.169645236058553 0.0 38 0.0010016487137828867 0.0 0.0 21.627198368514573 0.0 39 0.0010016487137828867 0.0 0.0 22.12722140643499 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACGAC 35 1.2100827E-7 45.0 25 CTCACGA 45 3.8380676E-10 45.0 24 TTTACGG 20 7.029448E-4 45.0 2 ATTACGG 25 3.8876242E-5 45.0 2 CGTTCGA 20 7.029448E-4 45.0 18 TTGCGCA 20 7.029448E-4 45.0 1 TCGTAAG 20 7.029448E-4 45.0 1 CGGTCTA 35 1.2100827E-7 45.0 31 TAGGCGG 35 1.2100827E-7 45.0 2 TTTCTCG 20 7.029448E-4 45.0 12 GCGTATT 20 7.029448E-4 45.0 12 ATAGCGG 50 1.0786607E-9 40.5 2 CACGGGT 90 0.0 40.0 4 CGTAAGG 90 0.0 40.0 2 TGCGAAG 80 0.0 39.375 1 TTACGGG 155 0.0 39.193546 3 TTTAGCG 35 6.241189E-6 38.571426 1 GCGCGAC 35 6.241189E-6 38.571426 9 TAGCGCG 35 6.241189E-6 38.571426 1 ACGGGCG 70 0.0 38.571426 5 >>END_MODULE