FastQCFastQC Report
Sat 14 Jan 2017
SRR2935829.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935829.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences624922
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45380.7261706260941365No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTC35030.560549956634588No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCG24610.3938091473815932No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGC24530.39252898761765465No Hit
GCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC17470.2795548884500786TruSeq Adapter, Index 19 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC10950.1752218676890876TruSeq Adapter, Index 19 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG9940.15905985066936354No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTT9810.1569795910529634No Hit
CGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTG9770.15633951117099415TruSeq Adapter, Index 16 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTC8800.14081757403323936No Hit
CTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGCT8670.13873731441683923TruSeq Adapter, Index 16 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCT8170.13073631589222334No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC7000.11201397934462222No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACGG406.8066583E-945.02
TAGACGG351.2107375E-745.02
TTACGGG1050.045.03
GCTAACG207.0308126E-445.01
TAGTACG207.0308126E-445.01
TAGCCGT302.1639062E-644.99999644
AGTACGG900.042.52
CGTTAGG750.042.02
CGGGTAT650.041.538466
CGTTTTT21400.041.2149541
CGTTATT2950.041.186441
CACGTGA1550.040.6451643
TGTACGG750.039.02
CACGACC1800.038.7527
TAATACG1050.038.571434
AATACGG1050.038.571435
CGGTCTA356.243714E-638.57142631
CCGAAAC356.243714E-638.57142631
TACGGGA2000.038.254
TACGCGG659.094947E-1238.076922
Produced by FastQC (version 0.11.5)