##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935829.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	624922
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00281955188007	33.0	31.0	34.0	30.0	34.0
2	32.117534668326606	33.0	31.0	34.0	30.0	34.0
3	32.05104637058705	33.0	31.0	34.0	30.0	34.0
4	35.72009146741513	37.0	35.0	37.0	33.0	37.0
5	32.03463632261306	37.0	35.0	37.0	0.0	37.0
6	33.818775783217745	37.0	35.0	37.0	17.0	37.0
7	35.476885115262384	37.0	35.0	37.0	32.0	37.0
8	35.71487321617738	37.0	35.0	37.0	35.0	37.0
9	37.64641827300047	39.0	37.0	39.0	35.0	39.0
10	37.33379205724874	39.0	37.0	39.0	34.0	39.0
11	37.275864827930526	39.0	37.0	39.0	34.0	39.0
12	37.13142120136593	39.0	37.0	39.0	34.0	39.0
13	37.03934891074406	39.0	37.0	39.0	33.0	39.0
14	38.035170789314506	40.0	37.0	41.0	33.0	41.0
15	38.20473595104669	40.0	37.0	41.0	33.0	41.0
16	38.22002265882782	40.0	37.0	41.0	33.0	41.0
17	38.211656814770485	40.0	37.0	41.0	33.0	41.0
18	38.10746941218264	40.0	37.0	41.0	33.0	41.0
19	37.97902778266727	40.0	37.0	41.0	34.0	41.0
20	37.99415607067762	40.0	36.0	41.0	33.0	41.0
21	37.945607611829956	40.0	36.0	41.0	33.0	41.0
22	38.04162439472446	40.0	36.0	41.0	34.0	41.0
23	38.05991947795085	40.0	36.0	41.0	34.0	41.0
24	38.02258361843558	40.0	36.0	41.0	34.0	41.0
25	37.780070152755066	40.0	35.0	41.0	33.0	41.0
26	37.81705396833525	40.0	35.0	41.0	34.0	41.0
27	37.79880209050089	40.0	35.0	41.0	34.0	41.0
28	37.73349474014357	40.0	35.0	41.0	33.0	41.0
29	37.7022380393073	40.0	35.0	41.0	33.0	41.0
30	37.52201714774004	40.0	35.0	41.0	33.0	41.0
31	37.47782283228947	40.0	35.0	41.0	33.0	41.0
32	37.36873401800545	40.0	35.0	41.0	33.0	41.0
33	37.26896316660319	40.0	35.0	41.0	33.0	41.0
34	37.09969244161671	40.0	35.0	41.0	32.0	41.0
35	37.030890255103834	40.0	35.0	41.0	32.0	41.0
36	36.93660008769094	40.0	35.0	41.0	31.0	41.0
37	36.905916258349045	40.0	35.0	41.0	31.0	41.0
38	36.79640179094351	39.0	35.0	41.0	31.0	41.0
39	36.68983809179385	39.0	35.0	41.0	31.0	41.0
40	36.59398292906955	39.0	35.0	41.0	31.0	41.0
41	36.46087671741434	39.0	35.0	41.0	30.0	41.0
42	36.483652359814506	39.0	35.0	41.0	31.0	41.0
43	36.417527307407966	39.0	35.0	41.0	31.0	41.0
44	36.36983015480332	39.0	35.0	41.0	31.0	41.0
45	36.35107421406192	39.0	35.0	41.0	31.0	41.0
46	36.25055126879835	39.0	35.0	41.0	30.0	41.0
47	36.161076102297564	39.0	35.0	41.0	30.0	41.0
48	36.12672621543169	38.0	35.0	41.0	30.0	41.0
49	36.11908366164097	38.0	35.0	41.0	30.0	41.0
50	36.00101292641322	38.0	35.0	40.0	30.0	41.0
51	34.97615542419694	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	4.0
9	22.0
10	28.0
11	17.0
12	16.0
13	11.0
14	17.0
15	26.0
16	46.0
17	81.0
18	168.0
19	315.0
20	583.0
21	925.0
22	1502.0
23	2203.0
24	3238.0
25	4646.0
26	5833.0
27	6845.0
28	7223.0
29	7980.0
30	9531.0
31	12095.0
32	16151.0
33	24978.0
34	42960.0
35	46835.0
36	47609.0
37	72375.0
38	118774.0
39	191765.0
40	117.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.53131750842505	19.457148252101863	22.83004919013893	13.181485049334157
2	34.76625882910187	24.61427186112827	23.41060164308506	17.208867666684803
3	31.639628625652485	24.78485314967308	27.341492218228836	16.234026006445603
4	27.75690406162689	24.55714473166251	28.922489526692928	18.763461680017667
5	23.62534844348575	35.454824762130315	23.552219316970756	17.36760747741318
6	25.02024252626728	33.98824173256823	26.065172933582108	14.926342807582385
7	77.51879434553433	5.329785157187617	12.576449540902704	4.574970956375355
8	77.79082829537126	6.185251919439546	11.013694509074732	5.0102252761144594
9	71.54572890696758	6.7296398590544095	13.146120635855354	8.578510598122646
10	40.24406245899488	25.733451534751538	19.426424417767336	14.596061588486242
11	31.464406757963392	22.335107421406192	26.61772189169208	19.58276392893833
12	27.384057530379792	20.15995596250412	31.1934929479199	21.262493559196187
13	26.596439235616604	21.170322056192614	33.53346497642906	18.699773731761724
14	21.078150553189037	24.81637708386007	33.13181485049334	20.973657512457557
15	19.51779582091845	23.61446708549227	36.28308812939855	20.58464896419073
16	25.286355737196004	23.779447675069846	30.159123858657562	20.775072729076587
17	24.57698720800356	24.018677530955863	29.548487651258874	21.8558476097817
18	25.18042251673009	22.67482981876138	31.60906481128845	20.535682853220084
19	23.752724339997634	25.02024252626728	28.72502488310541	22.50200825062968
20	23.55285939685273	27.66025199944953	29.057066321876967	19.729822281820773
21	23.74040280226972	26.938881972470163	30.428917528907608	18.891797696352505
22	22.175247470884365	22.514969868239557	31.983831582181455	23.325951078694622
23	20.780833448014313	25.725130496285935	31.649549863823008	21.844486191876747
24	22.445041141134414	25.041045122431278	29.910932884423975	22.602980852010333
25	23.284985966248588	26.533391367242633	27.71673904903332	22.46488361747546
26	21.00006080758879	24.315674596189606	29.348942748054956	25.335321848166654
27	23.236979975100894	23.005911137709987	30.560133904711307	23.196974982477812
28	20.16475656161889	24.50865868060334	30.242974323195536	25.083610434582237
29	22.057792812543006	24.863742995125794	30.273378117589072	22.80508607474213
30	25.110493789624943	22.9057386361818	29.732830657266028	22.250936916927234
31	24.173096802480952	22.490166772813247	28.961854439434042	24.374881985271763
32	24.58274792694128	23.149769091182577	29.753473233459538	22.5140097484166
33	24.255187047343508	21.65422244696138	28.47683390887183	25.613756596823283
34	21.033665001392173	22.165486252684335	31.62730708792457	25.173541657998914
35	20.966136573844416	23.796249771971542	31.604424232144172	23.633189422039873
36	22.371111914766963	24.55618461183956	29.55888894934088	23.513814524052602
37	21.35866556146207	24.667078451390733	29.80035908481378	24.17389690233341
38	21.253212400907636	24.25390688757957	29.206524974316793	25.286355737196004
39	21.400430773760565	21.968661688978784	30.01414576539152	26.61676177186913
40	23.284985966248588	20.98565901024448	30.961784030647028	24.76757099285991
41	20.05930340106445	22.900137937214566	29.96261933489299	27.077939326827988
42	20.922451121900014	22.73051676849271	32.31299266148415	24.034039448123128
43	21.816802736981575	22.36455109597678	31.129804999663957	24.68884116737769
44	22.01890795971337	21.905773840575304	29.96117915515856	26.114139044552758
45	21.175602715218858	21.22808926554034	29.253410825671043	28.342897193569755
46	23.05439718876916	22.32966674240945	29.220446711749627	25.39548935707176
47	20.0292196466119	23.591424209741376	32.37556046994665	24.00379567370008
48	20.50031843974128	23.85833752052256	30.643664329308294	24.99767971042786
49	22.383753492435858	21.114475086490796	32.15345275090331	24.348318670170038
50	20.73986833556828	22.091557026316885	31.3704750352844	25.798099602830433
51	20.113230131120364	21.283136135389697	29.723549498977476	28.880084234512466
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	202.0
1	426.0
2	650.0
3	4178.0
4	7706.0
5	4957.5
6	2209.0
7	2130.5
8	2052.0
9	2016.5
10	1981.0
11	1972.0
12	1963.0
13	1944.5
14	1926.0
15	1886.5
16	1847.0
17	1830.5
18	1814.0
19	1702.0
20	1590.0
21	1708.0
22	1826.0
23	2041.5
24	2257.0
25	2661.5
26	3600.5
27	4135.0
28	4658.5
29	5182.0
30	6308.5
31	7435.0
32	8858.5
33	10282.0
34	11640.5
35	12999.0
36	14538.5
37	16078.0
38	17177.0
39	18276.0
40	19957.5
41	21639.0
42	24647.0
43	27655.0
44	32248.0
45	36841.0
46	48514.5
47	60188.0
48	61323.0
49	62458.0
50	63304.5
51	64151.0
52	58148.0
53	52145.0
54	47321.5
55	42498.0
56	39745.0
57	36992.0
58	33954.5
59	30917.0
60	27498.0
61	24079.0
62	21566.0
63	19053.0
64	16532.0
65	14011.0
66	11858.0
67	9705.0
68	8126.5
69	6548.0
70	5331.5
71	4115.0
72	3442.0
73	2769.0
74	2310.0
75	1417.5
76	984.0
77	728.5
78	473.0
79	345.0
80	217.0
81	144.5
82	72.0
83	66.5
84	61.0
85	37.0
86	13.0
87	9.5
88	6.0
89	4.5
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	624922.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.27580488359931
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.68774068822071	26.722182344401784
2	10.298981100667893	7.678056200167465
3	4.1444082958834505	4.634584649859654
4	2.2793854600396504	3.3986371065180503
5	1.5706190247731493	2.927304415695647
6	1.253583175138273	2.80369931050903
7	1.0456341102302706	2.7283797150805675
8	0.8967445424290584	2.6741499675214495
9	0.7448054169107565	2.498689925731188
>10	5.989021961451852	36.49811222353286
>50	0.05021453819249719	1.3279924180306735
>100	0.031438667376379306	2.1579266420510796
>500	0.0048031297380579496	1.3791003498339718
>1k	0.002619888948031609	2.571184731066594
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4538	0.7261706260941365	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTC	3503	0.560549956634588	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCG	2461	0.3938091473815932	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGC	2453	0.39252898761765465	No Hit
GCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	1747	0.2795548884500786	TruSeq Adapter, Index 19 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	1095	0.1752218676890876	TruSeq Adapter, Index 19 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	994	0.15905985066936354	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTT	981	0.1569795910529634	No Hit
CGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTG	977	0.15633951117099415	TruSeq Adapter, Index 16 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTC	880	0.14081757403323936	No Hit
CTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGCT	867	0.13873731441683923	TruSeq Adapter, Index 16 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCT	817	0.13073631589222334	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	700	0.11201397934462222	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6001997049231744E-4	0.0	0.0	0.14337789356111644	0.0
2	1.6001997049231744E-4	0.0	0.0	0.7119288487203203	0.0
3	3.200399409846349E-4	0.0	0.0	1.0676532431247419	0.0
4	3.200399409846349E-4	0.0	0.0	1.5981194453067742	0.0
5	3.200399409846349E-4	0.0	0.0	3.239284262675982	0.0
6	3.200399409846349E-4	0.0	0.0	4.447595059863471	0.0
7	3.200399409846349E-4	0.0	0.0	5.507567344404582	0.0
8	3.200399409846349E-4	0.0	0.0	6.964709195707624	0.0
9	3.200399409846349E-4	0.0	0.0	7.76400894831675	0.0
10	3.200399409846349E-4	0.0	0.0	9.382770969817033	0.0
11	3.200399409846349E-4	0.0	0.0	10.777665052598564	0.0
12	3.200399409846349E-4	0.0	0.0	12.319297448321551	0.0
13	3.200399409846349E-4	0.0	0.0	12.846883291034722	0.0
14	3.200399409846349E-4	0.0	0.0	13.170923731281665	0.0
15	3.200399409846349E-4	0.0	0.0	13.579614735919042	0.0
16	3.200399409846349E-4	0.0	0.0	14.1491258109012	0.0
17	3.200399409846349E-4	0.0	0.0	14.82024956714598	0.0
18	3.200399409846349E-4	0.0	0.0	15.520816997961346	0.0
19	3.200399409846349E-4	0.0	0.0	16.084087294094303	0.0
20	4.800599114769523E-4	0.0	0.0	16.60383215825335	0.0
21	4.800599114769523E-4	0.0	0.0	17.15638111636332	0.0
22	4.800599114769523E-4	0.0	0.0	17.788620019778467	0.0
23	4.800599114769523E-4	0.0	0.0	18.376853431308227	1.6001997049231744E-4
24	4.800599114769523E-4	0.0	0.0	18.85003248405401	1.6001997049231744E-4
25	6.400798819692697E-4	0.0	0.0	19.283846624058683	1.6001997049231744E-4
26	6.400798819692697E-4	0.0	0.0	19.710299845420707	1.6001997049231744E-4
27	8.000998524615872E-4	0.0	0.0	20.219643411497756	1.6001997049231744E-4
28	8.000998524615872E-4	0.0	0.0	20.66529902931886	1.6001997049231744E-4
29	8.000998524615872E-4	0.0	0.0	21.14023830174006	1.6001997049231744E-4
30	8.000998524615872E-4	0.0	0.0	21.81504251730616	1.6001997049231744E-4
31	8.000998524615872E-4	0.0	0.0	22.31910542435696	1.6001997049231744E-4
32	8.000998524615872E-4	0.0	0.0	22.863493363971823	1.6001997049231744E-4
33	8.000998524615872E-4	0.0	0.0	23.36659615119967	1.6001997049231744E-4
34	8.000998524615872E-4	0.0	0.0	23.889701434739056	1.6001997049231744E-4
35	8.000998524615872E-4	0.0	0.0	24.424328156153887	1.6001997049231744E-4
36	8.000998524615872E-4	0.0	0.0	24.908228546922658	1.6001997049231744E-4
37	8.000998524615872E-4	0.0	0.0	25.407170814917702	1.6001997049231744E-4
38	8.000998524615872E-4	0.0	0.0	26.000524865503216	1.6001997049231744E-4
39	8.000998524615872E-4	0.0	0.0	26.97296622618503	1.6001997049231744E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTACGG	40	6.8066583E-9	45.0	2
TAGACGG	35	1.2107375E-7	45.0	2
TTACGGG	105	0.0	45.0	3
GCTAACG	20	7.0308126E-4	45.0	1
TAGTACG	20	7.0308126E-4	45.0	1
TAGCCGT	30	2.1639062E-6	44.999996	44
AGTACGG	90	0.0	42.5	2
CGTTAGG	75	0.0	42.0	2
CGGGTAT	65	0.0	41.53846	6
CGTTTTT	2140	0.0	41.214954	1
CGTTATT	295	0.0	41.18644	1
CACGTGA	155	0.0	40.64516	43
TGTACGG	75	0.0	39.0	2
CACGACC	180	0.0	38.75	27
TAATACG	105	0.0	38.57143	4
AATACGG	105	0.0	38.57143	5
CGGTCTA	35	6.243714E-6	38.571426	31
CCGAAAC	35	6.243714E-6	38.571426	31
TACGGGA	200	0.0	38.25	4
TACGCGG	65	9.094947E-12	38.07692	2
>>END_MODULE