##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935828.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	127971
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.911831586843896	33.0	31.0	34.0	30.0	34.0
2	32.02104383024279	33.0	31.0	34.0	30.0	34.0
3	31.987317439107297	33.0	31.0	34.0	30.0	34.0
4	35.65262442272077	37.0	35.0	37.0	33.0	37.0
5	32.20086582116261	37.0	35.0	37.0	10.0	37.0
6	33.885106782005295	37.0	35.0	37.0	19.0	37.0
7	35.45565792249807	37.0	35.0	37.0	32.0	37.0
8	35.61710856365895	37.0	35.0	37.0	33.0	37.0
9	37.573137664002	39.0	37.0	39.0	35.0	39.0
10	37.285619398144895	39.0	37.0	39.0	34.0	39.0
11	37.24003875878129	39.0	37.0	39.0	34.0	39.0
12	37.030819482539016	39.0	35.0	39.0	34.0	39.0
13	36.78595150463777	39.0	35.0	39.0	33.0	39.0
14	37.930382664822496	40.0	36.0	41.0	33.0	41.0
15	38.06024802494315	40.0	36.0	41.0	33.0	41.0
16	38.102195028561155	40.0	36.0	41.0	34.0	41.0
17	38.08891858311649	40.0	36.0	41.0	34.0	41.0
18	38.073813598393386	40.0	36.0	41.0	34.0	41.0
19	37.94774597369717	40.0	36.0	41.0	34.0	41.0
20	37.95643544240492	40.0	36.0	41.0	34.0	41.0
21	37.96721913558541	40.0	36.0	41.0	34.0	41.0
22	38.0552312633331	40.0	36.0	41.0	34.0	41.0
23	38.04610419548179	40.0	36.0	41.0	34.0	41.0
24	38.01808222175337	40.0	36.0	41.0	34.0	41.0
25	37.79861062271921	40.0	35.0	41.0	34.0	41.0
26	37.77530065405443	40.0	35.0	41.0	34.0	41.0
27	37.75488977971572	40.0	35.0	41.0	33.0	41.0
28	37.705597361902306	40.0	36.0	41.0	33.0	41.0
29	37.71101265130381	40.0	36.0	41.0	33.0	41.0
30	37.608465980573726	40.0	36.0	41.0	33.0	41.0
31	37.58216314633784	40.0	35.0	41.0	33.0	41.0
32	37.42698736432473	40.0	35.0	41.0	33.0	41.0
33	37.324917364090304	39.0	35.0	41.0	33.0	41.0
34	37.27167092544405	40.0	35.0	41.0	33.0	41.0
35	37.27597658844582	40.0	35.0	41.0	33.0	41.0
36	37.119527080354146	39.0	35.0	41.0	32.0	41.0
37	37.02738120355393	39.0	35.0	41.0	32.0	41.0
38	36.907807237577266	39.0	35.0	41.0	32.0	41.0
39	36.8814731462597	39.0	35.0	41.0	32.0	41.0
40	36.81402036398872	39.0	35.0	41.0	31.0	41.0
41	36.716435754975734	39.0	35.0	41.0	31.0	41.0
42	36.72155410210126	39.0	35.0	41.0	31.0	41.0
43	36.6069421978417	39.0	35.0	41.0	31.0	41.0
44	36.601956693313326	39.0	35.0	41.0	31.0	41.0
45	36.6383633792031	39.0	35.0	41.0	31.0	41.0
46	36.51927389799251	39.0	35.0	41.0	31.0	41.0
47	36.340788147314626	39.0	35.0	40.0	31.0	41.0
48	36.35121238405576	39.0	35.0	40.0	31.0	41.0
49	36.36839596471076	39.0	35.0	40.0	31.0	41.0
50	36.23113830477217	38.0	35.0	40.0	31.0	41.0
51	35.05783341538317	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	2.0
10	2.0
11	3.0
12	0.0
13	3.0
14	1.0
15	3.0
16	7.0
17	10.0
18	23.0
19	54.0
20	101.0
21	198.0
22	325.0
23	438.0
24	578.0
25	721.0
26	919.0
27	1049.0
28	1239.0
29	1450.0
30	1866.0
31	2548.0
32	3331.0
33	5181.0
34	8774.0
35	12757.0
36	9370.0
37	14337.0
38	24027.0
39	38628.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.57852169632182	19.677895773261127	22.16986661040392	11.573715920013129
2	30.56161161513155	28.245461862453215	24.320353830164645	16.872572692250586
3	29.980229895835777	28.47832712098835	26.02230192778051	15.519141055395364
4	26.067624696220236	24.218768314696298	31.46181556758953	18.25179142149393
5	24.66027459346258	34.35622133139539	23.063819146525386	17.91968492861664
6	22.57386439115112	36.23946050277016	25.41200740792836	15.774667698150363
7	74.48328136843504	4.952684592603012	14.885403724281282	5.678630314680669
8	72.47892100554031	8.977815286275796	11.142368192793679	7.400895515390206
9	67.72237460049543	6.125606582741402	13.358495284087802	12.793523532675371
10	39.81917778246634	23.904634643786483	20.740636550468466	15.535551023278712
11	31.503231200818938	22.301146353470706	25.479210133545884	20.716412312164476
12	28.157942033742017	19.007431371170032	30.72961842917536	22.10500816591259
13	23.716310726649006	27.05925561259973	33.00747825679255	16.21695540395871
14	17.878269295387238	32.529244907049254	28.368927335099357	21.22355846246415
15	15.209695946737934	23.976525931656393	40.830344374897436	19.983433746708236
16	16.05754428737761	25.231497761211525	33.358338998679386	25.352618952731476
17	16.961655375045908	23.864000437599145	29.97163419837307	29.20270998898188
18	18.78472466418173	24.854068499894506	32.70584741855577	23.655359417367997
19	19.516921802595903	26.019176219612255	29.09721733830321	25.366684639488636
20	21.96825843355135	25.883989341335145	31.885349024388336	20.262403200725167
21	19.709934281985763	29.03079603972775	30.602245821318892	20.657023856967594
22	16.84287846465215	25.136945089121753	29.898961483461093	28.121214962765002
23	16.77098717678224	28.109493557134037	29.845043017558666	25.27447624852506
24	21.25637839823085	24.260965374967768	30.0450883403271	24.43756788647428
25	16.881168389713295	30.130263887912108	27.98055809519344	25.00800962718116
26	16.892108368302196	29.15113580420564	29.488712286377382	24.468043541114785
27	22.54026302834236	28.118870681638807	27.238202405232435	22.1026638847864
28	17.049956630799166	27.178813950035558	33.148916551406174	22.6223128677591
29	18.07362605590329	26.68104492424065	30.96482796883669	24.28050105101937
30	22.182369443076947	26.95923295121551	30.263887912105087	20.594509693602454
31	25.690195434903217	26.516163818365097	23.00911925358089	24.78452149315079
32	24.999413929718454	31.262551671863157	24.001531597002447	19.736502801415945
33	22.77390971391956	24.539153401942627	28.52364988942807	24.16328699470974
34	21.164951434309337	25.966820607793956	26.977205773182988	25.891022184713723
35	23.012244961749147	24.661837447546713	27.214759593970513	25.111157996733635
36	25.573762805635653	24.693094529229278	30.30764782646068	19.425494838674386
37	23.342007173500246	25.61752271999125	30.638191465253843	20.40227864125466
38	22.389447609223964	26.08325323706152	27.59140742824546	23.93589172546905
39	20.605449672191355	25.208836376991663	31.00936931023435	23.176344640582634
40	23.412335607286025	22.417578982738277	30.84605105844293	23.32403435153277
41	20.49292417813411	23.350602870962952	27.801611302560737	28.3548616483422
42	25.08771518547171	21.77680880824562	29.94897281415321	23.186503192129468
43	24.82828140750639	23.041939189347588	29.464488048073388	22.665291355072632
44	21.770557391909104	24.034351532769143	29.577013542130636	24.618077533191112
45	21.03445311828461	23.473286916567034	27.64454446710583	27.847715498042525
46	26.235631510264046	24.203139773855014	27.79926702143454	21.7619616944464
47	19.40361488149659	23.219323127896164	34.08272186667291	23.29434012393433
48	21.547069257878736	21.57676348547718	30.15370669917403	26.72246055747005
49	21.468926553672315	19.36688781051957	35.3103437497558	23.85384188605231
50	22.042494002547453	20.45228997194677	32.377648060888795	25.127567964616983
51	20.37492869478241	20.870353439451126	27.35932359675239	31.39539426901407
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	102.0
1	149.5
2	197.0
3	390.5
4	584.0
5	382.0
6	180.0
7	186.5
8	193.0
9	208.5
10	224.0
11	261.5
12	299.0
13	266.5
14	234.0
15	260.5
16	287.0
17	271.5
18	256.0
19	234.5
20	213.0
21	245.0
22	277.0
23	330.5
24	384.0
25	475.0
26	692.5
27	819.0
28	855.5
29	892.0
30	1193.5
31	1495.0
32	1965.0
33	2435.0
34	2535.5
35	2636.0
36	2674.5
37	2713.0
38	3369.5
39	4026.0
40	4737.5
41	5449.0
42	6587.5
43	7726.0
44	9359.5
45	10993.0
46	15131.5
47	19270.0
48	17761.5
49	16253.0
50	16152.0
51	16051.0
52	12788.5
53	9526.0
54	8201.5
55	6877.0
56	5826.5
57	4776.0
58	4187.0
59	3598.0
60	3298.0
61	2998.0
62	2489.5
63	1981.0
64	1605.0
65	1229.0
66	1034.0
67	839.0
68	680.0
69	521.0
70	463.0
71	405.0
72	309.5
73	214.0
74	186.5
75	105.5
76	52.0
77	31.0
78	10.0
79	11.5
80	13.0
81	7.0
82	1.0
83	9.5
84	18.0
85	9.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	127971.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.463073665127254
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.8629999420637	29.887240077830135
2	11.168189104111546	9.037985168514743
3	4.167551804716015	5.0589586703237455
4	1.9949402290415406	3.2288565378093472
5	1.2919796836677546	2.613873455704808
6	0.9482242521388153	2.302084065921185
7	0.7242038585581584	2.0512459854185714
8	0.5832255074255036	1.88792773362715
9	0.5252891987408509	1.912933398973205
>10	4.623317433035283	31.829867704401778
>50	0.06179872926362952	1.7503965742238474
>100	0.036692995500280025	2.788913113127193
>500	0.003862420578976845	1.1080635456470607
>1k	0.00772484115795369	4.541653968477233
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGC	1521	1.1885505309796751	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTC	1519	1.1869876768955467	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCG	1440	1.1252549405724734	No Hit
GCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCTGC	1332	1.040860820029538	No Hit
CCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCTGC	823	0.6431144556188512	No Hit
CTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCTGCT	595	0.46494909002820956	Illumina Single End Adapter 2 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCT	329	0.25708949683912763	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	308	0.24067952895577902	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGGATCGTT	300	0.23442811261926527	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTC	264	0.20629673910495344	No Hit
TCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCTGC	254	0.19848246868431127	No Hit
GAACTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCT	254	0.19848246868431127	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGGATCGTTCGTA	243	0.1898867712216049	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCC	182	0.1422197216556876	No Hit
CGCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCTG	182	0.1422197216556876	No Hit
ACTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCTGC	169	0.1320611701088528	No Hit
GAATATGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTC	136	0.10627407772073359	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	129	0.10080408842628409	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4860476201639434	0.0
2	0.0	0.0	0.0	2.6052777582421016	0.0
3	0.0	0.0	0.0	3.3140320853943472	0.0
4	0.0	0.0	0.0	4.3228543966992525	0.0
5	0.0	0.0	0.0	7.704089207711122	0.0
6	0.0	0.0	0.0	8.972345296981347	0.0
7	0.0	0.0	0.0	10.575052160255058	0.0
8	0.0	0.0	0.0	12.798212094927758	0.0
9	0.0	0.0	0.0	13.546819201225277	0.0
10	0.0	0.0	0.0	16.399809331801737	0.0
11	0.0	0.0	0.0	18.83708027600003	0.0
12	0.0	0.0	0.0	22.104226738870526	0.0
13	0.0	0.0	0.0	23.009900680622955	0.0
14	0.0	0.0	0.0	23.369357119972495	0.0
15	0.0	0.0	0.0	24.12812277781685	0.0
16	0.0	0.0	0.0	25.412788834970424	0.0
17	0.0	0.0	0.0	26.817013229559823	0.0
18	0.0	0.0	0.0	28.39158871931922	0.0
19	0.0	0.0	0.0	29.520750795102014	0.0
20	0.0	0.0	0.0	30.378757687288527	0.0
21	0.0	0.0	0.0	31.34850864649022	0.0
22	0.0	0.0	0.0	32.31982245977604	0.0
23	0.0	0.0	0.0	33.33645904150159	0.0
24	0.0	0.0	0.0	33.984262059372824	0.0
25	0.0	0.0	0.0	34.70864492736636	0.0
26	0.0	0.0	0.0	35.35801079932172	0.0
27	0.0	0.0	0.0	35.9722124543842	0.0
28	0.0	0.0	0.0	36.635644013096716	0.0
29	0.0	0.0	0.0	37.23109141914965	0.0
30	0.0	0.0	0.0	37.957818568269374	0.0
31	0.0	0.0	0.0	38.60015159684616	0.0
32	0.0	0.0	0.0	39.158090504880015	0.0
33	0.0	0.0	0.0	39.70352658024083	0.0
34	0.0	0.0	0.0	40.23880410405482	0.0
35	0.0	0.0	0.0	40.93427417149198	0.0
36	0.0	0.0	0.0	41.4687702682639	0.0
37	0.0	0.0	0.0	42.015769197708856	0.0
38	0.0	0.0	0.0	42.56120527306968	0.0
39	0.0	0.0	0.0	43.09413851575748	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTTAG	30	2.1503547E-6	45.000004	1
CGGAAGG	20	7.009779E-4	45.000004	2
ATTAGCG	20	7.009779E-4	45.000004	1
ATAGGGC	20	7.009779E-4	45.000004	4
CGCGGGC	30	2.1503547E-6	45.000004	4
GGATGCG	20	7.009779E-4	45.000004	1
GACACCT	20	7.009779E-4	45.000004	13
ATACTTA	20	7.009779E-4	45.000004	45
TGCATCA	20	7.009779E-4	45.000004	18
AACGGGA	20	7.009779E-4	45.000004	4
TGCGACG	20	7.009779E-4	45.000004	1
ATTGGGC	20	7.009779E-4	45.000004	4
AGTTAGG	30	2.1503547E-6	45.000004	2
ACCAATC	20	7.009779E-4	45.000004	40
GTCTTGA	20	7.009779E-4	45.000004	15
TGATGCG	20	7.009779E-4	45.000004	1
GGGATTA	30	2.1503547E-6	45.000004	7
TCAACCA	20	7.009779E-4	45.000004	37
ATGGGCG	25	3.8713355E-5	45.0	5
TTAGGGT	25	3.8713355E-5	45.0	4
>>END_MODULE