##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935827.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	406110
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09451380167935	33.0	31.0	34.0	30.0	34.0
2	32.198436384230874	33.0	31.0	34.0	30.0	34.0
3	32.12648543498067	33.0	31.0	34.0	30.0	34.0
4	35.767001551303835	37.0	35.0	37.0	35.0	37.0
5	32.14507645711753	37.0	35.0	37.0	0.0	37.0
6	33.90743148408067	37.0	35.0	37.0	17.0	37.0
7	35.53575632217872	37.0	35.0	37.0	32.0	37.0
8	35.769047794932405	37.0	35.0	37.0	35.0	37.0
9	37.72180689960848	39.0	37.0	39.0	35.0	39.0
10	37.32400827362045	39.0	37.0	39.0	34.0	39.0
11	37.319691709142845	39.0	37.0	39.0	34.0	39.0
12	37.32091797788777	39.0	37.0	39.0	34.0	39.0
13	37.306316022752455	39.0	37.0	39.0	34.0	39.0
14	38.55411341754697	40.0	38.0	41.0	34.0	41.0
15	38.67552879761641	40.0	38.0	41.0	35.0	41.0
16	38.58792937873975	40.0	38.0	41.0	34.0	41.0
17	38.568279530176554	40.0	38.0	41.0	34.0	41.0
18	38.48023195685898	40.0	38.0	41.0	34.0	41.0
19	38.31753465809756	40.0	37.0	41.0	34.0	41.0
20	38.394041023368054	40.0	37.0	41.0	34.0	41.0
21	38.325549727906235	40.0	37.0	41.0	34.0	41.0
22	38.38036738814607	40.0	37.0	41.0	34.0	41.0
23	38.37324370244515	40.0	37.0	41.0	34.0	41.0
24	38.35442121592672	40.0	37.0	41.0	34.0	41.0
25	38.10317894166605	40.0	37.0	41.0	34.0	41.0
26	38.16652138583142	40.0	37.0	41.0	34.0	41.0
27	38.139799561695106	40.0	37.0	41.0	34.0	41.0
28	38.08166752850213	40.0	37.0	41.0	34.0	41.0
29	38.07018295535692	40.0	37.0	41.0	34.0	41.0
30	37.90098249242816	40.0	37.0	41.0	33.0	41.0
31	37.88404619438083	40.0	37.0	41.0	33.0	41.0
32	37.781554258698385	40.0	37.0	41.0	33.0	41.0
33	37.667875698702325	40.0	37.0	41.0	33.0	41.0
34	37.565548742951414	40.0	37.0	41.0	33.0	41.0
35	37.521508950776884	40.0	37.0	41.0	33.0	41.0
36	37.41873384058506	40.0	36.0	41.0	33.0	41.0
37	37.3737780404324	40.0	36.0	41.0	33.0	41.0
38	37.24286769594445	40.0	36.0	41.0	32.0	41.0
39	37.16134052350348	40.0	36.0	41.0	32.0	41.0
40	37.10798059639014	40.0	36.0	41.0	32.0	41.0
41	37.006559799069215	40.0	35.0	41.0	31.0	41.0
42	36.9946467705794	40.0	35.0	41.0	32.0	41.0
43	36.9256383738396	40.0	35.0	41.0	31.0	41.0
44	36.88345275910468	40.0	35.0	41.0	31.0	41.0
45	36.842397380020195	39.0	35.0	41.0	31.0	41.0
46	36.70029548644456	39.0	35.0	41.0	31.0	41.0
47	36.62088842924331	39.0	35.0	41.0	31.0	41.0
48	36.59594444854842	39.0	35.0	41.0	31.0	41.0
49	36.5568762158036	39.0	35.0	41.0	31.0	41.0
50	36.44811750510945	39.0	35.0	41.0	31.0	41.0
51	35.430528674497054	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	11.0
9	14.0
10	11.0
11	15.0
12	10.0
13	3.0
14	13.0
15	12.0
16	35.0
17	52.0
18	97.0
19	169.0
20	342.0
21	517.0
22	811.0
23	1326.0
24	1848.0
25	2577.0
26	3358.0
27	3761.0
28	4130.0
29	4565.0
30	5465.0
31	6621.0
32	8886.0
33	12800.0
34	21038.0
35	28168.0
36	29655.0
37	46770.0
38	80778.0
39	142146.0
40	106.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.12619733569723	19.521804437221444	22.692127748639532	12.659870478441801
2	34.9087685602423	24.096919553815468	23.281623205535446	17.712688680406785
3	32.17256408362266	24.648740489030065	26.763192238556055	16.415503188791213
4	28.059885252764026	25.023762034916647	27.49008889217207	19.426263820147252
5	23.435029918002513	36.27662455984832	22.849966757774986	17.438378764374185
6	25.102312181428676	34.82258501391249	25.208687301470047	14.86641550318879
7	78.47799857181552	6.034818152717244	10.892369062569255	4.594814212897983
8	79.19750806431755	6.462042303809312	9.791682056585655	4.548767575287484
9	73.22203343921598	7.826697200265937	11.719731107335452	7.231538253182636
10	35.56942700253626	29.837236216788554	19.493486986284502	15.099849794390682
11	28.35783408436138	26.065844229395978	26.525079411981977	19.05124227426067
12	24.46726256432986	24.763733963704414	29.724951367856	21.044052104109724
13	24.48942404767181	25.214104552953632	31.310236142916942	18.986235256457608
14	21.231193518997316	27.80773682007338	29.829356578266975	21.131713082662333
15	20.582108295781932	26.55191943069612	32.3404988796139	20.525473393908054
16	23.006082095983846	27.07665410849277	29.181502548570588	20.735761246952798
17	22.759104676072987	26.4226441112014	29.19898549654035	21.61926571618527
18	24.44091502302332	24.597522838639776	30.00541725148359	20.956144886853316
19	22.640417620841642	27.883085863436012	27.974194183841817	21.502302331880525
20	24.85361109059122	26.318485139494225	29.18347245820098	19.644431311713575
21	23.28359311516584	28.16995395336239	29.08867055723819	19.45778237423358
22	22.91251138854005	24.317795671123587	29.562187584644555	23.207505355691808
23	20.970426731673683	28.05052818201965	29.675703627096105	21.30334145921056
24	21.351850483859053	25.975228386397774	30.691192041565092	21.981729088178078
25	20.988894634458642	28.40880549604787	28.939449902735713	21.662849966757776
26	21.163970352860062	26.523848218462977	29.452365122749995	22.859816305926966
27	22.058555563763512	26.50045554160203	29.24897195341164	22.19201694122282
28	19.796114353254044	27.388146068799095	30.5956514244909	22.22008815345596
29	21.27083795030903	25.535199822708133	29.77986259880328	23.414099628179557
30	21.709635320479673	25.23429612666519	31.265913176233042	21.790155376622096
31	22.477407598926398	25.685897909433404	29.011844081652754	22.82485040998744
32	22.84848932555219	26.041466597719833	29.158848587821034	21.951195488906947
33	21.712836423629067	25.663982664795252	29.308315481027307	23.314865430548373
34	20.459235182586	26.2369801285366	29.830095294378374	23.47368939449903
35	22.081455763216862	25.769865307429026	29.337125409371843	22.81155351998227
36	20.83918150254857	28.364482529363965	28.625249316687594	22.171086651399868
37	21.41759621777351	27.101031740168917	29.542488488340595	21.938883553716973
38	21.509935731698306	27.421388293812022	29.524759301667036	21.543916672822636
39	22.368816330550835	26.268744921326736	28.18226588855236	23.180172859570067
40	23.456945162640665	24.494102583044004	29.597645957991674	22.451306296323654
41	21.001945285760016	26.57703577848366	28.051759375538648	24.369259560217674
42	22.249144320504296	26.324641107089214	28.87321169141366	22.553002880992835
43	22.194725566964614	26.081111029031547	28.961611386077664	22.762552017926176
44	21.769225086799143	25.452709856935314	28.915318509763367	23.86274654650218
45	22.09573760803723	24.538671788431706	28.642486025953563	24.723104577577505
46	22.448843909285664	25.9158848587821	27.964344635689837	23.6709265962424
47	20.720740686021028	25.926719361749278	30.80298441309005	22.549555539139643
48	21.11398389598877	25.678018270911824	29.898549654034625	23.309448179064784
49	22.099677427298023	24.74378862869666	30.71089113786905	22.445642806136267
50	20.796828445495063	24.878481199674965	30.481396665927953	23.84329368890202
51	20.974612789638275	23.800940631848512	28.90423776809239	26.32020881042082
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	133.0
1	304.0
2	475.0
3	2078.5
4	3682.0
5	2459.0
6	1236.0
7	1185.5
8	1135.0
9	1151.0
10	1167.0
11	1166.0
12	1165.0
13	1179.0
14	1193.0
15	1181.0
16	1169.0
17	1162.0
18	1155.0
19	1210.5
20	1266.0
21	1299.5
22	1333.0
23	1538.0
24	1743.0
25	2059.0
26	2984.0
27	3593.0
28	4114.0
29	4635.0
30	5667.0
31	6699.0
32	7769.0
33	8839.0
34	9899.5
35	10960.0
36	12343.5
37	13727.0
38	14883.5
39	16040.0
40	17534.0
41	19028.0
42	21021.5
43	23015.0
44	26079.0
45	29143.0
46	33813.0
47	38483.0
48	39162.5
49	39842.0
50	39554.5
51	39267.0
52	35669.0
53	32071.0
54	28741.0
55	25411.0
56	22615.0
57	19819.0
58	17647.0
59	15475.0
60	14095.5
61	12716.0
62	11054.0
63	9392.0
64	8126.0
65	6860.0
66	5465.0
67	4070.0
68	3459.5
69	2849.0
70	2390.5
71	1932.0
72	1558.0
73	1184.0
74	1045.0
75	718.5
76	531.0
77	374.0
78	217.0
79	156.0
80	95.0
81	77.0
82	59.0
83	39.0
84	19.0
85	10.5
86	2.0
87	1.5
88	1.0
89	2.0
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	406110.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.40992280073165
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.72735244588887	28.9849677503924
2	9.578915627733023	7.741664820628269
3	4.003336408978804	4.853235456965751
4	2.476094966567838	4.002352257851462
5	1.8562820199041195	3.750610656035584
6	1.5089177947241605	3.6585150958472146
7	1.2885791362666121	3.644996839341709
8	1.1279417500581126	3.6464031234856384
9	0.9961284801398727	3.6228127483856163
>10	5.373231244145311	31.431952515225486
>50	0.036827257627171026	0.9995653583061412
>100	0.0214825669491831	1.6379974258694185
>500	0.0024551505084780684	0.6510816539835288
>1k	0.0024551505084780684	1.3738442976818155
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1970	0.48509024648494253	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTC	1439	0.35433749476742754	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGC	1070	0.2634754130654256	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCG	1060	0.261013026027431	No Hit
GCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGC	957	0.23565043953608628	No Hit
CCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGC	573	0.1410947772770924	No Hit
CGCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTG	557	0.137154958016301	No Hit
CTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGCT	538	0.1324764226441112	Illumina Single End Adapter 2 (95% over 21bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	414	0.10194282337297776	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTT	407	0.10021915244638152	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.462387037994632E-4	0.0	0.0	0.13937110635049618	0.0
2	2.462387037994632E-4	0.0	0.0	0.6384969589520081	0.0
3	2.462387037994632E-4	0.0	0.0	0.9462953387013371	0.0
4	2.462387037994632E-4	0.0	0.0	1.3740119672010047	0.0
5	2.462387037994632E-4	0.0	0.0	2.611853931200906	0.0
6	2.462387037994632E-4	0.0	0.0	3.4842776587624043	0.0
7	2.462387037994632E-4	0.0	0.0	4.183841816256679	0.0
8	2.462387037994632E-4	0.0	0.0	5.17421388293812	0.0
9	2.462387037994632E-4	0.0	0.0	5.546526803082909	0.0
10	2.462387037994632E-4	0.0	0.0	6.646475092955111	0.0
11	2.462387037994632E-4	0.0	0.0	7.758735318017286	0.0
12	2.462387037994632E-4	0.0	0.0	8.893649503829012	0.0
13	2.462387037994632E-4	0.0	0.0	9.317180074364089	0.0
14	2.462387037994632E-4	0.0	0.0	9.489054689616113	0.0
15	2.462387037994632E-4	0.0	0.0	9.805717662702223	0.0
16	2.462387037994632E-4	0.0	0.0	10.377237694220778	0.0
17	4.924774075989264E-4	0.0	0.0	11.066459826155475	0.0
18	4.924774075989264E-4	0.0	0.0	11.745093693826796	0.0
19	4.924774075989264E-4	0.0	0.0	12.241757159390312	0.0
20	4.924774075989264E-4	0.0	0.0	12.68375563271035	0.0
21	4.924774075989264E-4	0.0	0.0	13.208490310507006	0.0
22	4.924774075989264E-4	0.0	0.0	13.828519366674055	0.0
23	7.387161113983896E-4	0.0	0.0	14.414321243012976	0.0
24	7.387161113983896E-4	0.0	0.0	14.87084779985718	0.0
25	7.387161113983896E-4	0.0	0.0	15.30348920243284	0.0
26	7.387161113983896E-4	0.0	0.0	15.69747112851198	0.0
27	7.387161113983896E-4	0.0	0.0	16.126665189234444	0.0
28	9.849548151978527E-4	0.0	0.0	16.553396862918913	0.0
29	9.849548151978527E-4	0.0	0.0	16.997119007165548	0.0
30	9.849548151978527E-4	0.0	0.0	17.538597916820567	0.0
31	9.849548151978527E-4	0.0	0.0	17.993647041441974	0.0
32	9.849548151978527E-4	0.0	0.0	18.456083327177367	0.0
33	9.849548151978527E-4	0.0	0.0	18.911132451798775	0.0
34	9.849548151978527E-4	0.0	0.0	19.342050183447835	0.0
35	0.001231193518997316	0.0	0.0	19.785526088990668	0.0
36	0.001231193518997316	0.0	0.0	20.203393169338355	0.0
37	0.001231193518997316	0.0	0.0	20.61929034005565	0.0
38	0.001231193518997316	0.0	0.0	21.058826426337692	0.0
39	0.001231193518997316	0.0	0.0	21.5138755509591	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCACGC	20	7.027892E-4	45.000004	2
TGCGACT	20	7.027892E-4	45.000004	34
TCCGTCG	20	7.027892E-4	45.000004	1
CGTAAGG	45	3.8380676E-10	45.0	2
CGTTATT	145	0.0	41.896553	1
ACGGGCG	65	0.0	41.53846	5
TACGGGA	120	0.0	41.250004	4
CGTTTTT	995	0.0	41.15578	1
TAATACG	55	6.002665E-11	40.909092	4
CGTTAGG	55	6.002665E-11	40.909092	2
CGGGTCA	50	1.0786607E-9	40.5	6
CGCACGG	45	1.9230356E-8	40.0	2
ATGGGCG	125	0.0	39.600002	5
CGGGTCT	80	0.0	39.375004	6
TACGAGG	40	3.4508594E-7	39.375004	2
ACATACG	40	3.4508594E-7	39.375004	17
TAAACGG	35	6.2383133E-6	38.57143	2
ACGGGTA	35	6.2383133E-6	38.57143	5
TGCGAAG	35	6.2383133E-6	38.57143	1
TTAACGG	35	6.2383133E-6	38.57143	2
>>END_MODULE