##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935826.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	240725
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.958782843493612	33.0	31.0	34.0	30.0	34.0
2	32.07702980579499	33.0	31.0	34.0	30.0	34.0
3	32.022631633606814	33.0	31.0	34.0	30.0	34.0
4	35.680041541177694	37.0	35.0	37.0	33.0	37.0
5	32.282691868314465	37.0	35.0	37.0	10.0	37.0
6	33.93256205213418	37.0	35.0	37.0	19.0	37.0
7	35.49466818984318	37.0	35.0	37.0	32.0	37.0
8	35.69138228268771	37.0	35.0	37.0	33.0	37.0
9	37.61253297330979	39.0	37.0	39.0	35.0	39.0
10	37.18491224426212	39.0	37.0	39.0	34.0	39.0
11	37.184442828954204	39.0	37.0	39.0	34.0	39.0
12	37.07891992938	39.0	37.0	39.0	34.0	39.0
13	36.98712638903313	39.0	37.0	39.0	33.0	39.0
14	38.12824176965417	40.0	37.0	41.0	33.0	41.0
15	38.2671305431509	40.0	37.0	41.0	34.0	41.0
16	38.26087028767265	40.0	37.0	41.0	34.0	41.0
17	38.23904455291307	40.0	37.0	41.0	34.0	41.0
18	38.19649392460276	40.0	37.0	41.0	34.0	41.0
19	38.07656869872261	40.0	37.0	41.0	34.0	41.0
20	38.08572022016824	40.0	37.0	41.0	34.0	41.0
21	38.07722089521238	40.0	37.0	41.0	34.0	41.0
22	38.141730190050886	40.0	37.0	41.0	34.0	41.0
23	38.14038425589366	40.0	37.0	41.0	34.0	41.0
24	38.09794578876311	40.0	36.0	41.0	34.0	41.0
25	37.87521030221207	40.0	36.0	41.0	34.0	41.0
26	37.91464534219545	40.0	36.0	41.0	34.0	41.0
27	37.87725412815453	40.0	36.0	41.0	34.0	41.0
28	37.8451178730917	40.0	36.0	41.0	33.0	41.0
29	37.82610447606189	40.0	36.0	41.0	33.0	41.0
30	37.66235746183404	40.0	36.0	41.0	33.0	41.0
31	37.649143213210095	40.0	36.0	41.0	33.0	41.0
32	37.55078201267006	40.0	36.0	41.0	33.0	41.0
33	37.458523211133034	40.0	36.0	41.0	33.0	41.0
34	37.40826669436078	40.0	36.0	41.0	33.0	41.0
35	37.38612524665074	40.0	36.0	41.0	33.0	41.0
36	37.3318973932911	40.0	35.0	41.0	33.0	41.0
37	37.27114342091598	40.0	35.0	41.0	33.0	41.0
38	37.10248623948489	40.0	35.0	41.0	32.0	41.0
39	37.03668916813792	40.0	35.0	41.0	32.0	41.0
40	36.98730917021498	39.0	35.0	41.0	32.0	41.0
41	36.89235019212795	39.0	35.0	41.0	31.0	41.0
42	36.90024717000727	39.0	35.0	41.0	31.0	41.0
43	36.82293488420397	39.0	35.0	41.0	31.0	41.0
44	36.768694568491014	39.0	35.0	41.0	31.0	41.0
45	36.72451137189739	39.0	35.0	41.0	31.0	41.0
46	36.59029598089106	39.0	35.0	41.0	31.0	41.0
47	36.45394121923356	39.0	35.0	41.0	31.0	41.0
48	36.415727489874335	39.0	35.0	41.0	31.0	41.0
49	36.32008308235538	39.0	35.0	40.0	31.0	41.0
50	36.18325059715443	38.0	35.0	40.0	31.0	41.0
51	35.071579603281755	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	6.0
9	7.0
10	15.0
11	7.0
12	4.0
13	6.0
14	8.0
15	5.0
16	19.0
17	21.0
18	49.0
19	95.0
20	226.0
21	402.0
22	623.0
23	817.0
24	1009.0
25	1421.0
26	1777.0
27	2001.0
28	2324.0
29	2654.0
30	3400.0
31	4438.0
32	5979.0
33	8868.0
34	14936.0
35	20543.0
36	18254.0
37	28157.0
38	47437.0
39	75166.0
40	47.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.01921279468273	21.02357461834043	24.839547201163153	11.117665385813687
2	34.73424031571295	26.302212067712123	22.54522795721259	16.418319659362343
3	30.5871845466819	26.32381348011216	25.79748675874961	17.29151521445633
4	28.56745248727802	25.050160972063555	27.67764046110707	18.704746079551356
5	24.359746598816077	35.82012670059196	22.171357357981098	17.648769342610862
6	24.802990964793853	35.75739952227646	24.926783674317164	14.512825838612525
7	77.66746287257243	5.579810987641499	12.130023886177172	4.62270225360889
8	77.38124415827188	7.381867275937274	9.389552393810364	5.8473361719804755
9	71.77401599335342	6.869664554990133	11.389760099698828	9.966559351957628
10	40.59279260567037	22.521964897704848	20.774327552186104	16.110914944438676
11	32.20895212379271	24.3236057742237	23.851282583861252	19.616159518122338
12	26.558105722297228	21.583134281856893	30.520303250597152	21.338456745248727
13	24.504309897185585	25.105826150171357	32.09263682625402	18.297227126389032
14	20.58531519368574	29.93332640980372	29.664139578357045	19.817218818153496
15	18.911621144459446	25.14362862187143	36.046526119015475	19.89822411465365
16	21.441063454148924	24.50264825007789	31.72624363900717	22.330044656766017
17	21.215079447502337	24.8873195555094	29.293592273340952	24.604008723647315
18	22.11693841520407	25.450617925018175	30.884203967182472	21.548239692595285
19	22.341676186519887	27.00508879426732	28.120469415307923	22.53276560390487
20	23.576279987537646	26.796136670474606	29.49091286737979	20.136670474607953
21	22.364939246027625	28.64804237200125	29.512929691556756	19.474088690414373
22	21.392875688025757	24.315712950462146	28.8212690829785	25.470142278533597
23	19.821372935922735	27.256828331083188	29.341780039464123	23.58001869352996
24	21.93249558624987	25.131581680340638	29.94661958666528	22.989303146744213
25	20.04486447190778	28.42538165957005	27.994184235123065	23.535569633399106
26	20.051926472115483	26.845986083705476	29.554886281026064	23.547201163152977
27	21.68491016720324	27.35818880465261	27.700903520614812	23.25599750752934
28	19.598296811714615	26.132308650950254	30.609201370858862	23.66019316647627
29	22.290580537958252	25.038529442309688	29.08007062000208	23.59081939972998
30	21.22712638903313	28.55208225153183	27.22359538892928	22.997195970505764
31	24.79634437636307	26.541489251220273	25.72354346245716	22.9386229099595
32	23.581264928860733	28.67130543150898	25.282791567140926	22.464638072489354
33	22.49828642642019	28.252154948592796	24.80672967078617	24.442828954200852
34	22.786997611382283	25.93249558624987	28.060234707653965	23.220272094713884
35	20.574099075708794	26.88171149652093	26.425589365458514	26.118600062311764
36	23.93685740990757	28.155364004569527	25.8564752310728	22.0513033544501
37	21.29899262644096	26.94236161595181	28.833731436286218	22.924914321321012
38	20.490185896770175	30.19337418215806	25.20054003531	24.11589988576176
39	21.227541800810055	29.217156506386953	27.860837054730503	21.69446463807249
40	21.58479592896459	26.09616782635788	28.61023990030117	23.708796344376363
41	20.039464118807768	26.313012773912142	27.10686467961367	26.540658427666425
42	21.784193581888047	25.60847440024925	27.47741198462976	25.129920033232946
43	23.05909232526742	26.176342299304185	26.348322774950674	24.416242600477723
44	22.331706303873712	25.43317063038737	27.03333679509814	25.201786270640774
45	21.679925225880154	25.613874753349258	26.580953370028038	26.12524665074255
46	24.14165541593104	25.93872676290373	27.23564233046007	22.683975490705162
47	20.00540035310001	25.34593415723336	30.905805379582514	23.74286011008412
48	21.181846505348425	24.787620729047667	28.756880257555302	25.273652508048606
49	21.389552393810366	23.60120469415308	31.358604216429537	23.65063869560702
50	21.745144874857203	24.09762176757711	29.9034167618652	24.25381659570049
51	20.49184754387787	24.03198670682314	26.778689375843808	28.69747637345519
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	124.0
1	210.5
2	297.0
3	680.5
4	1064.0
5	750.5
6	437.0
7	425.5
8	414.0
9	446.0
10	478.0
11	479.5
12	481.0
13	533.5
14	586.0
15	535.0
16	484.0
17	480.5
18	477.0
19	517.5
20	558.0
21	587.5
22	617.0
23	757.0
24	897.0
25	1110.0
26	1568.5
27	1814.0
28	2057.5
29	2301.0
30	2768.5
31	3236.0
32	3754.0
33	4272.0
34	4644.0
35	5016.0
36	5409.0
37	5802.0
38	6704.5
39	7607.0
40	9287.5
41	10968.0
42	12514.0
43	14060.0
44	16355.5
45	18651.0
46	23426.0
47	28201.0
48	27552.5
49	26904.0
50	26908.5
51	26913.0
52	23758.0
53	20603.0
54	17585.0
55	14567.0
56	12939.0
57	11311.0
58	9923.5
59	8536.0
60	7868.0
61	7200.0
62	6105.0
63	5010.0
64	4309.0
65	3608.0
66	2947.0
67	2286.0
68	1872.5
69	1459.0
70	1189.0
71	919.0
72	749.5
73	580.0
74	436.0
75	238.0
76	184.0
77	153.5
78	123.0
79	80.0
80	37.0
81	25.0
82	13.0
83	11.5
84	10.0
85	5.5
86	1.0
87	0.5
88	0.0
89	1.0
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	240725.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.310728009139055
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.20946433356005	29.511268044449064
2	10.518559739483502	8.480216014124
3	3.960304210721573	4.789282376155364
4	2.1908942888353016	3.5326617509606395
5	1.369566561553206	2.7604112576591544
6	1.0449514623137328	2.5273652508048605
7	0.8862507471299904	2.5007788970817324
8	0.7450689420639337	2.4027417177276975
9	0.663657536222923	2.407726659050784
>10	5.331931820523918	34.92325267421331
>50	0.048434633854778544	1.3347180392564129
>100	0.02473257898967415	1.8506594661958666
>500	0.0020610482491395126	0.5213417800394641
>1k	0.004122096498279025	2.457576072281649
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGC	1725	0.7165853151936857	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCG	1585	0.6584276664243431	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTC	1550	0.6438882542320075	No Hit
GCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGC	1056	0.43867483643161287	No Hit
CCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGC	718	0.29826565583134285	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	537	0.2230761242081213	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCT	344	0.14290165126181328	No Hit
TCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGC	323	0.13417800394641188	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCGAATCAT	293	0.1217156506386956	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTC	284	0.11797694464638073	No Hit
GAACTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCT	271	0.11257659154637034	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCGAATCATCGTA	262	0.10883788555405545	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCC	252	0.10468376778481671	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.154117769238758E-4	0.0	0.0	0.10676082666943608	0.0
2	4.154117769238758E-4	0.0	0.0	1.120365562363693	0.0
3	4.154117769238758E-4	0.0	0.0	1.5457472219337418	0.0
4	4.154117769238758E-4	0.0	0.0	2.1996053588119224	0.0
5	4.154117769238758E-4	0.0	0.0	4.344376363069893	0.0
6	4.154117769238758E-4	0.0	0.0	5.284868626025548	0.0
7	4.154117769238758E-4	0.0	0.0	6.250285595596635	0.0
8	4.154117769238758E-4	0.0	0.0	7.654377401599335	0.0
9	4.154117769238758E-4	0.0	0.0	8.131685533284868	0.0
10	4.154117769238758E-4	0.0	0.0	9.883892408349777	0.0
11	4.154117769238758E-4	0.0	0.0	11.668085990237824	0.0
12	8.308235538477516E-4	0.0	0.0	13.673278637449371	0.0
13	8.308235538477516E-4	0.0	0.0	14.260255478242808	0.0
14	8.308235538477516E-4	0.0	0.0	14.489978190881711	0.0
15	8.308235538477516E-4	0.0	0.0	15.031675147990445	0.0
16	8.308235538477516E-4	0.0	0.0	16.031986706823137	0.0
17	8.308235538477516E-4	0.0	0.0	17.108734032609824	0.0
18	8.308235538477516E-4	0.0	0.0	18.217883476996573	0.0
19	0.0012462353307716274	0.0	0.0	19.046629971959707	0.0
20	0.0012462353307716274	0.0	0.0	19.723751168345622	0.0
21	0.0012462353307716274	0.0	0.0	20.51884930937792	0.0
22	0.0012462353307716274	0.0	0.0	21.39993768823346	0.0
23	0.0012462353307716274	0.0	0.0	22.30968947969675	0.0
24	0.0016616471076955032	0.0	0.0	22.97019420500571	0.0
25	0.0016616471076955032	0.0	0.0	23.597465988160764	0.0
26	0.0016616471076955032	0.0	0.0	24.17655000519265	0.0
27	0.0016616471076955032	0.0	0.0	24.702876726555196	0.0
28	0.0016616471076955032	0.0	0.0	25.294007685117872	0.0
29	0.0016616471076955032	0.0	0.0	25.879738290580537	0.0
30	0.0016616471076955032	0.0	0.0	26.52196489770485	0.0
31	0.0016616471076955032	0.0	0.0	27.124727386021394	0.0
32	0.0016616471076955032	0.0	0.0	27.718766227022535	0.0
33	0.0016616471076955032	0.0	0.0	28.264617301900508	0.0
34	0.0016616471076955032	0.0	0.0	28.819192024093883	0.0
35	0.0016616471076955032	0.0	0.0	29.40284557067193	0.0
36	0.002077058884619379	0.0	0.0	29.96697476373455	0.0
37	0.002077058884619379	0.0	0.0	30.5227957212587	0.0
38	0.002077058884619379	0.0	0.0	31.11808079759061	0.0
39	0.002077058884619379	0.0	0.0	31.699241873507113	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATG	20	7.0221646E-4	45.0	6
TAATACG	40	6.7757355E-9	45.0	4
GACCGAA	20	7.0221646E-4	45.0	31
AGTGCGG	25	3.8815895E-5	45.0	2
TGACCGA	20	7.0221646E-4	45.0	30
CCTCCGC	25	3.8815895E-5	45.0	45
TTAAGCG	25	3.8815895E-5	45.0	1
CAAGCGA	35	1.2065902E-7	45.0	15
CCGACAA	20	7.0221646E-4	45.0	12
TTCGCAG	20	7.0221646E-4	45.0	1
TAACGGG	60	0.0	44.999996	3
GACGGGA	100	0.0	42.75	4
ACGGGAC	60	3.6379788E-12	41.249996	5
TAGTAGG	55	6.002665E-11	40.909092	2
AGTAAGG	105	0.0	40.714287	2
ACGGGAT	50	1.0732037E-9	40.5	5
TATTAGG	50	1.0732037E-9	40.5	2
CGTTTTT	380	0.0	40.263157	1
CTTGAGG	45	1.9173967E-8	40.0	2
GTACAGG	40	3.443056E-7	39.375	2
>>END_MODULE