##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935824.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	276885
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.998757606948733	33.0	31.0	34.0	30.0	34.0
2	32.103255864348014	33.0	31.0	34.0	30.0	34.0
3	32.04833053433736	33.0	31.0	34.0	30.0	34.0
4	35.70131281940156	37.0	35.0	37.0	33.0	37.0
5	32.02778048648356	37.0	35.0	37.0	0.0	37.0
6	33.83117178612059	37.0	35.0	37.0	17.0	37.0
7	35.48942340682955	37.0	35.0	37.0	32.0	37.0
8	35.725571988370625	37.0	35.0	37.0	35.0	37.0
9	37.70381566354263	39.0	37.0	39.0	35.0	39.0
10	37.16798670928364	39.0	37.0	39.0	34.0	39.0
11	37.19029561009083	39.0	37.0	39.0	34.0	39.0
12	37.09012767033245	39.0	37.0	39.0	34.0	39.0
13	36.99865287032522	39.0	37.0	39.0	33.0	39.0
14	38.16526716868014	40.0	37.0	41.0	33.0	41.0
15	38.318778554273436	40.0	37.0	41.0	34.0	41.0
16	38.28981346046192	40.0	37.0	41.0	34.0	41.0
17	38.300048756704044	40.0	37.0	41.0	34.0	41.0
18	38.25813243765462	40.0	37.0	41.0	34.0	41.0
19	38.148538201780525	40.0	37.0	41.0	34.0	41.0
20	38.20489734005093	40.0	37.0	41.0	34.0	41.0
21	38.189143507232245	40.0	37.0	41.0	34.0	41.0
22	38.2472940029254	40.0	37.0	41.0	34.0	41.0
23	38.26976542607942	40.0	37.0	41.0	34.0	41.0
24	38.22159741408888	40.0	37.0	41.0	34.0	41.0
25	37.999346299005005	40.0	36.0	41.0	34.0	41.0
26	38.03296675515106	40.0	36.0	41.0	34.0	41.0
27	38.03814218899543	40.0	36.0	41.0	34.0	41.0
28	37.99465482059339	40.0	36.0	41.0	34.0	41.0
29	37.96676598587862	40.0	36.0	41.0	34.0	41.0
30	37.851721833974395	40.0	36.0	41.0	34.0	41.0
31	37.86458999223505	40.0	36.0	41.0	34.0	41.0
32	37.81460172996009	40.0	36.0	41.0	34.0	41.0
33	37.76773750835184	40.0	36.0	41.0	33.0	41.0
34	37.70513751196345	40.0	36.0	41.0	33.0	41.0
35	37.744374018094156	40.0	36.0	41.0	33.0	41.0
36	37.66916228759232	40.0	36.0	41.0	33.0	41.0
37	37.637268179930295	40.0	36.0	41.0	33.0	41.0
38	37.51983675533164	40.0	36.0	41.0	33.0	41.0
39	37.4610433934666	40.0	36.0	41.0	33.0	41.0
40	37.38967080195749	40.0	35.0	41.0	33.0	41.0
41	37.32871769868357	40.0	35.0	41.0	33.0	41.0
42	37.35337775610813	40.0	35.0	41.0	33.0	41.0
43	37.31212597287683	40.0	35.0	41.0	33.0	41.0
44	37.282283258392475	40.0	35.0	41.0	33.0	41.0
45	37.27054914495188	40.0	35.0	41.0	33.0	41.0
46	37.159221337378334	39.0	35.0	41.0	32.0	41.0
47	37.07659497625368	39.0	35.0	41.0	32.0	41.0
48	37.07500948047023	39.0	35.0	41.0	32.0	41.0
49	37.05297867345649	39.0	35.0	41.0	32.0	41.0
50	36.92496523827582	39.0	35.0	41.0	32.0	41.0
51	35.91227404879282	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	6.0
10	10.0
11	12.0
12	4.0
13	4.0
14	2.0
15	5.0
16	13.0
17	28.0
18	43.0
19	108.0
20	204.0
21	327.0
22	544.0
23	773.0
24	1054.0
25	1302.0
26	1630.0
27	1848.0
28	2156.0
29	2585.0
30	3399.0
31	4713.0
32	6348.0
33	9797.0
34	17091.0
35	23260.0
36	19681.0
37	30811.0
38	53081.0
39	95979.0
40	64.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.23105260306626	19.931379453563753	24.01213500189609	10.825432941473897
2	32.57092294634957	25.438358885457863	24.14395868320783	17.84675948498474
3	31.70233129277498	26.286364375101577	25.407299059176193	16.604005272947255
4	27.113061379272985	25.968542896870545	27.70572620401972	19.212669519836755
5	23.553460823085395	36.893295050291634	21.757408310309334	17.795835816313634
6	23.849973815844123	36.927966484280475	24.467919894541055	14.754139805334345
7	79.15091102804413	5.960597359914766	10.16739801722737	4.721093594813731
8	79.79197139606697	6.624410856492768	8.194015566029218	5.389602181411055
9	75.50029795763584	5.886920562688481	10.009209599653285	8.603571880022393
10	39.92993481048089	23.075645123426693	20.445311230294166	16.549108835798258
11	31.887606768152843	25.530454881990718	22.77263123679506	19.80930711306138
12	28.590208931505863	21.92065297867346	28.227964678476624	21.26117341134406
13	24.938512378785415	25.113314191812485	29.813460461924624	20.134712967477473
14	20.212001372410928	29.43785326037886	28.14164725427524	22.208498112934976
15	19.940047312060962	24.948624880365493	34.68299113350308	20.42833667407046
16	20.818390306444915	24.467558733770336	31.923361684453834	22.790689275330912
17	21.168716254040486	24.781407443523484	28.879137548079527	25.170738754356503
18	21.47678639146216	25.25019412391426	30.331365007132927	22.941654477490655
19	22.375715549776984	27.515755638622537	27.7180056702241	22.390523141376384
20	23.760767105477004	26.988822074146306	29.03335319717572	20.21705762320097
21	22.355490546616828	28.328367372735975	28.663524567961428	20.652617512685772
22	20.441699622586995	26.507394766780436	27.144482366325366	25.906423244307202
23	19.89598569803348	28.381458006031384	28.019574913772864	23.70298138216227
24	21.88706502699677	25.829134839373747	29.103418386694834	23.180381746934646
25	19.76705130288748	28.359427199017645	27.531646712534087	24.341874785560794
26	19.061704317677012	29.25510591039601	28.535312494356862	23.14787727757011
27	20.95490907777597	28.431298192390344	27.322895787059608	23.290896942774076
28	18.212615345721147	27.771818625060945	31.058020477815703	22.957545551402205
29	20.66020188887083	26.824493923470033	28.916698268234104	23.59860591942503
30	20.50562507900392	28.079166440941183	27.82201997219062	23.593188507864276
31	21.316069848493058	27.495891796233092	27.965039637394582	23.222998717879264
32	24.047889918197086	28.53711829821045	25.384545930621016	22.03044585297145
33	21.675785976127273	26.89166982682341	26.684363544431804	24.748180652617513
34	20.282066561930044	26.097838452787258	30.593928887444243	23.026166097838452
35	21.57935605034581	26.065333983422722	26.17043176770139	26.184878198530075
36	20.526211242934792	27.48614045542373	27.928923560322875	24.0587247413186
37	20.68151037434314	25.95156834064684	29.97309352258158	23.393827762428447
38	21.208805099590084	28.348592375896132	26.611047907976236	23.831554616537552
39	22.214637846037164	26.271917944272893	27.959261065063114	23.55418314462683
40	22.555934774364808	25.624717843147877	29.456994781226864	22.36235260126045
41	19.73454683352294	25.288838326380986	28.159705292811093	26.816909547284972
42	21.51109666468028	24.942485147263305	28.63210358090904	24.91431460714737
43	22.412192787619407	25.055889629268467	27.132564060891706	25.399353522220412
44	21.08637159831699	25.86958484569406	27.75376058652509	25.290282969463856
45	21.424418079708182	25.422106650775593	26.914061794607868	26.239413474908357
46	23.403940264008526	26.3354822399191	27.452191342976327	22.80838615309605
47	19.283095870126587	25.727648662802245	30.95220037199559	24.037055095075573
48	21.093594813731332	24.055113133611428	29.208877331744226	25.642414720913013
49	21.49195514383228	22.40713653682937	31.214764252306914	24.886144067031438
50	20.580385358542355	23.5260126045109	29.793235458764467	26.10036657818228
51	19.6258374415371	23.72790147534175	28.00043339292486	28.645827690196292
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	84.0
1	166.0
2	248.0
3	520.0
4	792.0
5	534.0
6	276.0
7	300.5
8	325.0
9	343.0
10	361.0
11	370.5
12	380.0
13	389.5
14	399.0
15	388.5
16	378.0
17	406.5
18	435.0
19	419.5
20	404.0
21	539.0
22	674.0
23	691.5
24	709.0
25	992.5
26	1465.5
27	1655.0
28	2146.5
29	2638.0
30	3008.5
31	3379.0
32	4031.5
33	4684.0
34	5627.5
35	6571.0
36	7414.5
37	8258.0
38	8920.0
39	9582.0
40	11114.5
41	12647.0
42	15063.0
43	17479.0
44	20038.5
45	22598.0
46	27439.0
47	32280.0
48	31975.5
49	31671.0
50	31444.5
51	31218.0
52	27247.5
53	23277.0
54	19827.0
55	16377.0
56	14527.0
57	12677.0
58	11463.5
59	10250.0
60	9058.5
61	7867.0
62	7011.0
63	6155.0
64	4970.0
65	3785.0
66	3008.0
67	2231.0
68	1718.5
69	1206.0
70	942.5
71	679.0
72	544.5
73	410.0
74	349.0
75	224.5
76	161.0
77	121.5
78	82.0
79	53.5
80	25.0
81	18.0
82	11.0
83	6.0
84	1.0
85	1.0
86	1.0
87	0.5
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	276885.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.69380960240331
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.1822293391491	26.119471704453446
2	11.477281450616788	8.422903606042553
3	4.347171764356599	4.785428790907341
4	2.1772325022903556	3.1956381959682583
5	1.406491541113578	2.5804766408506223
6	1.017631608754934	2.240446829822455
7	0.8636873139166196	2.218438449700731
8	0.6797539034238762	1.995420824698093
9	0.5937850273518049	1.9609411264564476
>10	6.1667673968265095	40.774076995100074
>50	0.05697937131125013	1.3997290195510947
>100	0.02499095232949567	1.693173258450695
>500	0.0019992761863596538	0.5410378154711385
>1k	0.0039985523727193075	2.072816742527053
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGC	1512	0.5460750853242321	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCG	1445	0.5218773136861874	No Hit
GCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC	1350	0.48756704046806437	Illumina Single End Adapter 1 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTC	1344	0.4854000758437618	No Hit
CCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC	918	0.3315455875182838	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGCT	557	0.20116654928941619	Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	378	0.13651877133105803	No Hit
TCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC	375	0.13543528901890678	Illumina Single End Adapter 1 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCT	306	0.11051519583942793	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.223215414341695E-4	0.0	0.0	0.20947324701590914	0.0
2	7.223215414341695E-4	0.0	0.0	1.3016234176643733	0.0
3	7.223215414341695E-4	0.0	0.0	1.7194864293840404	0.0
4	7.223215414341695E-4	0.0	0.0	2.3247918811058743	0.0
5	7.223215414341695E-4	0.0	0.0	4.175379670260217	0.0
6	7.223215414341695E-4	0.0	0.0	5.072864185492172	0.0
7	7.223215414341695E-4	0.0	0.0	6.0061036170251185	0.0
8	7.223215414341695E-4	0.0	0.0	7.419686873611789	0.0
9	0.001083482312151254	0.0	0.0	7.8418838145800605	0.0
10	0.001083482312151254	0.0	0.0	9.50575148527367	0.0
11	0.001083482312151254	0.0	0.0	11.232461130072052	0.0
12	0.001083482312151254	0.0	0.0	13.089549813099302	0.0
13	0.001083482312151254	0.0	0.0	13.652599454647236	0.0
14	0.001083482312151254	0.0	0.0	13.90252270798346	0.0
15	0.001083482312151254	0.0	0.0	14.385755819202918	0.0
16	0.001083482312151254	0.0	0.0	15.381114903299204	0.0
17	0.001083482312151254	0.0	0.0	16.419452119110822	0.0
18	0.001083482312151254	0.0	0.0	17.522437112880798	0.0
19	0.0018058038535854236	0.0	0.0	18.278346605991658	0.0
20	0.0018058038535854236	0.0	0.0	18.928797154053125	0.0
21	0.0018058038535854236	0.0	0.0	19.75332719360023	0.0
22	0.0018058038535854236	0.0	0.0	20.651895191144337	0.0
23	0.0018058038535854236	0.0	0.0	21.537822561713348	0.0
24	0.0018058038535854236	0.0	0.0	22.262672228542534	0.0
25	0.0018058038535854236	0.0	0.0	22.904454918106794	0.0
26	0.0018058038535854236	0.0	0.0	23.497842064394966	0.0
27	0.0018058038535854236	0.0	0.0	24.06450331365007	0.0
28	0.0018058038535854236	0.0	0.0	24.63080340213446	0.0
29	0.0018058038535854236	0.0	0.0	25.189157953663074	0.0
30	0.0018058038535854236	0.0	0.0	25.829134839373747	0.0
31	0.0018058038535854236	0.0	0.0	26.41963269949618	0.0
32	0.0018058038535854236	0.0	0.0	26.97365332177619	0.0
33	0.0018058038535854236	0.0	0.0	27.55439984108926	0.0
34	0.0018058038535854236	0.0	0.0	28.078082958629032	0.0
35	0.0018058038535854236	0.0	0.0	28.693139751160228	0.0
36	0.0018058038535854236	0.0	0.0	29.250049659605974	0.0
37	0.0018058038535854236	0.0	0.0	29.79431894107662	0.0
38	0.0018058038535854236	0.0	0.0	30.331003846362208	0.0
39	0.0018058038535854236	0.0	0.0	30.85468696390198	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGG	35	1.2074634E-7	45.000004	2
AGTCCGG	20	7.023999E-4	45.000004	2
CGAACTT	20	7.023999E-4	45.000004	10
GCAAGCG	20	7.023999E-4	45.000004	1
TTGTGCG	20	7.023999E-4	45.000004	1
GCGAACT	20	7.023999E-4	45.000004	9
TCGGGCC	20	7.023999E-4	45.000004	5
GCCGGAC	20	7.023999E-4	45.000004	38
TTCGTAC	20	7.023999E-4	45.000004	33
ACTTACG	20	7.023999E-4	45.000004	1
CGCTCGA	20	7.023999E-4	45.000004	41
CGCTCAA	20	7.023999E-4	45.000004	14
TTTGGCG	20	7.023999E-4	45.000004	1
GTTCCCG	20	7.023999E-4	45.000004	17
TTAACGG	20	7.023999E-4	45.000004	2
TCCCGCT	20	7.023999E-4	45.000004	18
CTCGAGG	20	7.023999E-4	45.000004	2
GGCGACT	35	1.2074634E-7	45.000004	10
TTCGCAG	20	7.023999E-4	45.000004	1
GCGTCAT	40	6.7830115E-9	45.000004	37
>>END_MODULE