##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935823.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	337826
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.829992362932398	33.0	31.0	34.0	30.0	34.0
2	31.933974886480023	33.0	31.0	34.0	30.0	34.0
3	31.883792248080372	33.0	31.0	34.0	30.0	34.0
4	35.58422383120305	37.0	35.0	37.0	33.0	37.0
5	31.76352027375039	37.0	35.0	37.0	0.0	37.0
6	33.62999591505687	37.0	35.0	37.0	17.0	37.0
7	35.3382007305536	37.0	35.0	37.0	32.0	37.0
8	35.56300876782723	37.0	35.0	37.0	33.0	37.0
9	37.50778507278895	39.0	37.0	39.0	35.0	39.0
10	37.01158288586432	39.0	37.0	39.0	33.0	39.0
11	37.03919473338346	39.0	37.0	39.0	33.0	39.0
12	37.040668865037034	39.0	37.0	39.0	33.0	39.0
13	36.95719690017938	39.0	37.0	39.0	33.0	39.0
14	38.14657249590026	40.0	37.0	41.0	33.0	41.0
15	38.22082077756004	40.0	37.0	41.0	33.0	41.0
16	38.272296981286225	40.0	37.0	41.0	34.0	41.0
17	38.16947777850136	40.0	37.0	41.0	33.0	41.0
18	38.116438640009946	40.0	37.0	41.0	34.0	41.0
19	37.9736698773925	40.0	37.0	41.0	34.0	41.0
20	37.87194887308851	40.0	36.0	41.0	33.0	41.0
21	37.9795131221398	40.0	37.0	41.0	33.0	41.0
22	38.0689704167234	40.0	37.0	41.0	34.0	41.0
23	37.99783024397175	40.0	37.0	41.0	34.0	41.0
24	37.9955124827574	40.0	36.0	41.0	33.0	41.0
25	37.852924286467	40.0	36.0	41.0	33.0	41.0
26	37.82614422809375	40.0	36.0	41.0	33.0	41.0
27	37.74775772143056	40.0	36.0	41.0	33.0	41.0
28	37.70016517378769	40.0	36.0	41.0	33.0	41.0
29	37.67998022650713	40.0	36.0	41.0	33.0	41.0
30	37.551482123933624	40.0	36.0	41.0	33.0	41.0
31	37.53549164362719	40.0	36.0	41.0	33.0	41.0
32	37.31959055845317	40.0	36.0	41.0	32.0	41.0
33	37.244625931692646	40.0	36.0	41.0	32.0	41.0
34	37.09240555789075	40.0	36.0	41.0	31.0	41.0
35	36.99517503093308	40.0	36.0	41.0	31.0	41.0
36	36.92352276023752	40.0	36.0	41.0	31.0	41.0
37	36.798473178500174	40.0	36.0	41.0	31.0	41.0
38	36.7285792094155	40.0	35.0	41.0	30.0	41.0
39	36.67568215590274	40.0	35.0	41.0	30.0	41.0
40	36.65105705303914	40.0	35.0	41.0	30.0	41.0
41	36.6355757105729	40.0	35.0	41.0	30.0	41.0
42	36.6003919177328	40.0	35.0	41.0	30.0	41.0
43	36.554599113152925	40.0	35.0	41.0	30.0	41.0
44	36.59301829936121	40.0	35.0	41.0	30.0	41.0
45	36.64363903311172	39.0	35.0	41.0	30.0	41.0
46	36.54398418120571	39.0	35.0	41.0	30.0	41.0
47	36.35769893377064	39.0	35.0	41.0	30.0	41.0
48	36.40549276846661	39.0	35.0	41.0	30.0	41.0
49	36.422430481964085	39.0	35.0	41.0	30.0	41.0
50	36.27970020069503	39.0	35.0	41.0	30.0	41.0
51	35.36462261637648	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	5.0
10	16.0
11	3.0
12	5.0
13	2.0
14	5.0
15	9.0
16	18.0
17	30.0
18	81.0
19	206.0
20	507.0
21	949.0
22	1484.0
23	2053.0
24	2457.0
25	2983.0
26	3233.0
27	3450.0
28	3459.0
29	3841.0
30	4708.0
31	6220.0
32	8286.0
33	12064.0
34	18714.0
35	25295.0
36	25117.0
37	38606.0
38	64886.0
39	109066.0
40	66.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.84097730784487	19.854895715545872	23.291576136827835	10.012550839781426
2	31.49875971654047	29.513714160544186	23.456157903772947	15.531368219142399
3	27.46709844712959	30.057189203909708	26.783314487339638	15.692397861621071
4	25.23133210587699	23.24983867434715	34.420974110932846	17.097855108843014
5	26.13001959588664	34.67110287544475	22.919195088595902	16.2796824400727
6	21.88759894146691	38.59383232788477	25.56937595093332	13.949192779715
7	75.1626576995258	4.4043383280150135	16.197687566972345	4.235316405486849
8	75.19581086121258	9.801199434028169	10.024391254669563	4.978598450089692
9	70.58574532451617	5.406037427551461	11.248986164475202	12.759231083457165
10	37.57259654378289	25.382297395700746	21.325771255024776	15.719334805491583
11	29.027665129386133	24.704137632982658	26.872413609372874	19.395783628258336
12	23.23000597940952	20.760391444116202	32.419351974093175	23.590250602381108
13	23.907277711010995	24.18049528455477	35.687306483219174	16.224920521215065
14	20.613866309875498	30.50475688668131	29.48411312332384	19.39726368011935
15	17.473788281541385	25.685708027209277	37.34289249495302	19.49761119629632
16	19.763428510535007	25.592760770337392	30.01781982440665	24.62599089472095
17	20.336800601493078	26.874189671606093	30.648025906827776	22.140983820073053
18	20.24740546908764	25.516686104681106	31.807498534748657	22.4284098914826
19	19.75070006453026	28.305399821209736	29.135412904868186	22.808487209391817
20	21.913055833476406	27.451113887030605	32.1893519148911	18.446478364601894
21	19.89574514690995	31.338914115550608	30.33691900564196	18.428421731897483
22	18.03235985388928	24.34537306187209	31.718103402343218	25.904163681895415
23	18.98936138722301	28.411371534458567	30.2199949086216	22.379272169696826
24	19.80398193152688	26.83955645805829	30.076133867730725	23.280327742684104
25	17.66234688863498	32.33469300764299	28.24649375714125	21.756466346580783
26	17.117095783036238	27.252490927282093	31.800098275443574	23.8303150142381
27	21.723313184894	28.415219669297215	28.186995672328358	21.67447147348043
28	17.342063665910853	27.27824382966379	32.29413958665111	23.085552917774237
29	19.630519853415667	24.686673021022656	29.948257386938838	25.734549738622842
30	19.71725089247127	30.931307833026466	28.489518272720275	20.86192300178198
31	22.413609372872425	26.832452209125407	25.069118421909504	25.68481999609266
32	22.67913067673891	31.812530711076114	24.57330104846874	20.935037563716232
33	23.583442363820428	26.278616802732767	26.00687928105001	24.131061552396798
34	20.265166091419843	25.747278184627586	26.39702095161414	27.590534772338422
35	19.009490092532843	30.002131274679865	26.14689218710224	24.841486445685057
36	21.74225784871502	27.80780638553575	27.311989012094983	23.137946753654248
37	18.434637949713757	31.508232048450978	28.844138698619997	21.212991303215265
38	22.479027665129387	28.242645622302604	25.857394043087268	23.420932669480738
39	20.760095433744	27.887433175658476	27.818166748562867	23.534304642034655
40	21.263017056117647	26.42839805106771	27.80810239590795	24.50048249690669
41	17.42021040417256	26.98963371676544	28.129865670493093	27.46029020856891
42	19.21995346716949	27.9495953538212	30.030548270411394	22.799902908597915
43	21.261240993884424	25.64604263733401	28.82667408666	24.266042282121568
44	20.73375051061789	25.698732483586223	28.21541266805989	25.35210433773599
45	20.311047699111377	24.491010164996183	27.153031442221735	28.044910693670705
46	24.2690023858436	28.254486037190745	25.498037451232292	21.978474125733367
47	18.186877268179476	26.30200162213684	32.43711259642538	23.074008513258303
48	20.01829344100217	26.36978799737143	27.943675146377128	25.668243415249272
49	19.83003084428078	23.344561993452253	33.26978977343366	23.555617388833305
50	21.042489328826083	23.283583856778343	30.21969889824939	25.454227916146184
51	19.672849336640756	23.38422738332751	26.992001799743065	29.950921480288667
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	81.0
1	172.0
2	263.0
3	1366.5
4	2470.0
5	1716.5
6	963.0
7	970.0
8	977.0
9	970.5
10	964.0
11	958.5
12	953.0
13	943.5
14	934.0
15	864.5
16	795.0
17	806.5
18	818.0
19	819.0
20	820.0
21	904.0
22	988.0
23	1130.5
24	1273.0
25	1536.5
26	2203.5
27	2607.0
28	2950.0
29	3293.0
30	3958.0
31	4623.0
32	5573.0
33	6523.0
34	7732.0
35	8941.0
36	9849.0
37	10757.0
38	11792.0
39	12827.0
40	14474.0
41	16121.0
42	18325.5
43	20530.0
44	23961.5
45	27393.0
46	39127.0
47	50861.0
48	45304.0
49	39747.0
50	39257.0
51	38767.0
52	32728.5
53	26690.0
54	22714.0
55	18738.0
56	15628.0
57	12518.0
58	10366.5
59	8215.0
60	6980.0
61	5745.0
62	4785.0
63	3825.0
64	3097.5
65	2370.0
66	1865.0
67	1360.0
68	989.5
69	619.0
70	449.0
71	279.0
72	225.0
73	171.0
74	142.5
75	74.0
76	34.0
77	28.5
78	23.0
79	23.5
80	24.0
81	15.0
82	6.0
83	4.5
84	3.0
85	2.5
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	337826.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.10791065231376
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.09623056201349	34.32541969169172
2	10.340887010566304	9.329116146765935
3	3.8088608405259974	5.1542926344463025
4	2.1056749211700674	3.799303848278289
5	1.4391325564139397	3.2458131385777946
6	0.9922559503418551	2.685515565134825
7	0.7913018794017415	2.49857821325432
8	0.6025302144529824	2.174310326309166
9	0.5000824475759443	2.0301906927641467
>10	3.2408318117507466	24.219614524349986
>50	0.05171387783370738	1.5916225522894536
>100	0.020552951446729858	1.6544261479265794
>500	0.005966985903889313	1.8117342017605242
>1k	0.003977990602592876	5.480062316450948
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGC	4856	1.4374263674199144	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTC	4778	1.414337558388046	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCG	4412	1.3059977621615861	No Hit
GCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC	1991	0.589356651057053	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1236	0.36586882004345433	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCT	1051	0.3111069011858176	No Hit
GAACTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCT	911	0.26966544907733564	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAGAGCATT	847	0.25072078525631536	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTC	729	0.21579156133630922	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAGAGCATTCGTA	720	0.21312746798647825	No Hit
CCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC	635	0.18796658634918567	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGC	605	0.17908627518308243	No Hit
CTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGCT	567	0.1678378810393516	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTT	524	0.15510943503460362	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCG	520	0.15392539354578985	No Hit
GAATATGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTC	469	0.1388288645634143	No Hit
TCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC	374	0.11070787920408731	No Hit
CGCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTG	359	0.10626772362103568	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.96010372203442E-4	0.0	0.0	0.17523814034443766	0.0
2	2.96010372203442E-4	0.0	0.0	1.1908497273744472	0.0
3	2.96010372203442E-4	0.0	0.0	1.6002320721318075	0.0
4	2.96010372203442E-4	0.0	0.0	2.366010905022112	0.0
5	2.96010372203442E-4	0.0	0.0	5.108250993114798	0.0
6	2.96010372203442E-4	0.0	0.0	6.204969422128551	0.0
7	2.96010372203442E-4	0.0	0.0	7.3602979048385855	0.0
8	2.96010372203442E-4	0.0	0.0	8.927968836028015	0.0
9	2.96010372203442E-4	0.0	0.0	9.408689680486404	0.0
10	2.96010372203442E-4	0.0	0.0	12.12251277284756	0.0
11	2.96010372203442E-4	0.0	0.0	13.908935369095333	0.0
12	2.96010372203442E-4	0.0	0.0	16.871111163735176	0.0
13	2.96010372203442E-4	0.0	0.0	17.52411004481597	0.0
14	2.96010372203442E-4	0.0	0.0	17.789335338310256	0.0
15	2.96010372203442E-4	0.0	0.0	18.423389555570026	0.0
16	2.96010372203442E-4	0.0	0.0	19.417096375056982	0.0
17	2.96010372203442E-4	0.0	0.0	20.658267865706016	0.0
18	2.96010372203442E-4	0.0	0.0	21.865102153179446	0.0
19	2.96010372203442E-4	0.0	0.0	22.945244001349806	0.0
20	2.96010372203442E-4	0.0	0.0	23.686157962975024	0.0
21	5.92020744406884E-4	0.0	0.0	24.566196799535856	0.0
22	5.92020744406884E-4	0.0	0.0	25.625617921651976	0.0
23	5.92020744406884E-4	0.0	0.0	26.661950234736224	0.0
24	5.92020744406884E-4	0.0	0.0	27.457034094474672	0.0
25	5.92020744406884E-4	0.0	0.0	28.13400981570394	0.0
26	5.92020744406884E-4	0.0	0.0	28.77280019891897	0.0
27	8.880311166103261E-4	0.0	0.0	29.450367940892647	0.0
28	8.880311166103261E-4	0.0	0.0	30.12201547542226	0.0
29	8.880311166103261E-4	0.0	0.0	30.8395446176434	0.0
30	8.880311166103261E-4	0.0	0.0	31.623972103982524	0.0
31	8.880311166103261E-4	0.0	0.0	32.360149899652484	0.0
32	8.880311166103261E-4	0.0	0.0	33.04245380758142	0.0
33	8.880311166103261E-4	0.0	0.0	33.73334201630425	0.0
34	8.880311166103261E-4	0.0	0.0	34.38663690775725	0.0
35	8.880311166103261E-4	0.0	0.0	35.04822008963194	0.0
36	0.001184041488813768	0.0	0.0	35.69914689810731	0.0
37	0.001184041488813768	0.0	0.0	36.35451386216573	0.0
38	0.001184041488813768	0.0	0.0	37.007808753618725	0.0
39	0.001184041488813768	0.0	0.0	37.65725551023308	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGGCG	20	7.0262083E-4	45.0	1
CGGCGCC	40	6.7902874E-9	45.0	31
CCCTCGA	40	6.7902874E-9	45.0	41
CACGGGT	20	7.0262083E-4	45.0	4
GCGCCCA	20	7.0262083E-4	45.0	33
CTGCGCG	20	7.0262083E-4	45.0	1
TTAAGCG	20	7.0262083E-4	45.0	1
AGTTACG	20	7.0262083E-4	45.0	1
GACGATA	20	7.0262083E-4	45.0	23
TTCGCGG	20	7.0262083E-4	45.0	2
CGATCTA	20	7.0262083E-4	45.0	10
CGCCCAC	20	7.0262083E-4	45.0	34
CTTACGG	20	7.0262083E-4	45.0	2
TCACGGG	30	2.1609358E-6	44.999996	3
CGGGTGC	25	3.8849394E-5	44.999996	6
CGGCGCT	25	3.8849394E-5	44.999996	9
ACGCCGG	30	2.1609358E-6	44.999996	27
TACGGGA	120	0.0	44.999996	4
TCGATGG	25	3.8849394E-5	44.999996	2
CGTTAGG	25	3.8849394E-5	44.999996	2
>>END_MODULE