FastQCFastQC Report
Sat 14 Jan 2017
SRR2935821.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935821.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519587
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109692.1110997773231435No Hit
GAATCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTC42950.8266180639623394No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTT25530.4913517851678352No Hit
CGTTCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTC20490.3943516677668995No Hit
CGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTG17080.32872262008094894TruSeq Adapter, Index 21 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCT14180.27290906046533114No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCT13980.26905984945735745No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGC12730.2450022806575222No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12490.24038322744795387No Hit
GCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC11150.21459351369453047TruSeq Adapter, Index 15 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCG10000.19246055039868204No Hit
CGTCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCT9320.17937323297157165No Hit
GAATGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTT9150.17610140361479404No Hit
CCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC8630.1660934549940626TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTC6170.11874815959598681No Hit
CTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGCT5990.11528386968881053TruSeq Adapter, Index 21 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCC5490.10566084216887643No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATTC302.1631968E-645.0000049
GCAAACG207.029715E-445.01
CATACGA207.029715E-445.018
ACATACG207.029715E-445.017
GCGTTAG502.1827873E-1144.9999961
TGCGAAG502.1827873E-1144.9999961
CGTTTTT56950.044.4073751
CGTTATT7750.043.838711
TTACGGG1150.043.0434763
AACACGT1050.042.85714341
TCGCAAG650.041.538461
ACGACCA1200.041.25000428
ACACGAC1150.041.08695626
GTTATTT8550.039.736842
CACGACC1250.039.627
CGTTTCT3150.039.2857131
CAAACGG356.241682E-638.5714262
TTGGACG356.241682E-638.5714261
TAAACGG356.241682E-638.5714262
ACGGGCG1400.038.5714265
Produced by FastQC (version 0.11.5)