##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935821.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	519587
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.011070330858935	33.0	31.0	34.0	30.0	34.0
2	32.17784509620141	33.0	31.0	34.0	30.0	34.0
3	31.97510137859492	33.0	31.0	34.0	30.0	34.0
4	35.71007165306291	37.0	35.0	37.0	33.0	37.0
5	32.047651307673206	37.0	35.0	37.0	0.0	37.0
6	33.846060428763614	37.0	35.0	37.0	17.0	37.0
7	35.48831860689355	37.0	35.0	37.0	32.0	37.0
8	35.74227415235562	37.0	35.0	37.0	35.0	37.0
9	37.70501186519293	39.0	37.0	39.0	35.0	39.0
10	37.403728345782326	39.0	37.0	39.0	34.0	39.0
11	37.38586993131083	39.0	37.0	39.0	34.0	39.0
12	37.40348777009432	39.0	37.0	39.0	35.0	39.0
13	37.405497058240485	39.0	37.0	39.0	35.0	39.0
14	38.68575618712554	40.0	38.0	41.0	35.0	41.0
15	38.77738857977586	40.0	38.0	41.0	35.0	41.0
16	38.698524020038995	40.0	38.0	41.0	35.0	41.0
17	38.66969727880028	40.0	38.0	41.0	34.0	41.0
18	38.42789754170139	40.0	38.0	41.0	34.0	41.0
19	38.15670330474011	40.0	37.0	41.0	34.0	41.0
20	38.03620952795201	40.0	36.0	41.0	34.0	41.0
21	37.956971594747365	40.0	35.0	41.0	34.0	41.0
22	38.008672272400965	40.0	35.0	41.0	34.0	41.0
23	38.01455001761014	40.0	35.0	41.0	34.0	41.0
24	37.98051529387764	40.0	35.0	41.0	34.0	41.0
25	37.72134021828876	40.0	35.0	41.0	33.0	41.0
26	37.75958982807499	40.0	35.0	41.0	34.0	41.0
27	37.72446577762723	40.0	35.0	41.0	34.0	41.0
28	37.599683979776245	40.0	35.0	41.0	33.0	41.0
29	37.51330383554631	40.0	35.0	41.0	33.0	41.0
30	37.250031274839436	40.0	35.0	41.0	33.0	41.0
31	37.06957063975812	40.0	35.0	41.0	32.0	41.0
32	36.70637256128425	39.0	35.0	41.0	31.0	41.0
33	36.30326393847421	39.0	35.0	41.0	30.0	41.0
34	35.900184184746735	39.0	35.0	41.0	25.0	41.0
35	35.626260857180796	39.0	35.0	41.0	24.0	41.0
36	35.429512285719234	39.0	35.0	41.0	23.0	41.0
37	35.3753327161765	39.0	35.0	41.0	23.0	41.0
38	35.232838773872324	39.0	35.0	41.0	22.0	41.0
39	35.14100044843308	39.0	35.0	41.0	21.0	41.0
40	35.05956269113739	39.0	35.0	41.0	21.0	41.0
41	34.92018083593315	39.0	35.0	41.0	20.0	41.0
42	34.87168077723269	39.0	35.0	41.0	20.0	41.0
43	34.830657810915206	39.0	35.0	41.0	19.0	41.0
44	34.79297403514715	39.0	35.0	41.0	18.0	41.0
45	34.80171174413525	39.0	35.0	41.0	20.0	41.0
46	34.667432018122085	39.0	34.0	41.0	20.0	41.0
47	34.55765252017468	38.0	34.0	41.0	20.0	41.0
48	34.50635216046591	38.0	34.0	40.0	20.0	41.0
49	34.47071039113758	38.0	34.0	40.0	20.0	41.0
50	34.36737062320651	38.0	34.0	40.0	19.0	41.0
51	33.39245015752896	37.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	5.0
9	16.0
10	14.0
11	14.0
12	4.0
13	11.0
14	16.0
15	19.0
16	49.0
17	88.0
18	179.0
19	366.0
20	749.0
21	1222.0
22	1857.0
23	2952.0
24	4622.0
25	7782.0
26	10920.0
27	12081.0
28	11024.0
29	9732.0
30	9330.0
31	10314.0
32	12351.0
33	16615.0
34	26259.0
35	34326.0
36	38796.0
37	56978.0
38	96634.0
39	154156.0
40	103.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.674202780285114	16.941532409394384	20.44816363765837	22.93610117266213
2	41.681951242044164	22.41645768658569	20.807102564151915	15.094488507218232
3	28.246665139812965	22.78752162775435	34.77954606254583	14.186267169886852
4	24.602232157463526	22.02268340046999	36.69414361791192	16.68094082415457
5	20.951255516400526	33.54029257852872	29.590424702696566	15.918027202374194
6	22.099090238978267	30.670898232634762	33.18019888873278	14.049812639654185
7	69.99232082403908	4.751081147141865	20.707600459595792	4.548997569223248
8	70.91382193934798	5.276690910280665	19.235277249045875	4.574209901325476
9	64.95216393019841	6.201078933845535	20.854832780650785	7.9919243553052715
10	31.69690542681014	26.832465015483454	27.394449822647605	14.076179735058805
11	24.38840848597059	22.70880526264129	35.379638058688926	17.5231481926992
12	21.2035712979732	20.618106303660408	38.475173551301324	19.703148847065073
13	21.229553472277022	20.86407088706992	40.501205765348246	17.40516987530481
14	18.103609212701627	25.231963078368015	38.12585765232772	18.538570056602648
15	17.80625766233566	24.276203985088156	39.75330406649897	18.16423428607721
16	20.370217114746904	24.854355478485797	36.74360597936438	18.03182142740292
17	20.234147505615034	25.020448933479862	35.76090240902871	18.984501151876394
18	20.561330441292796	23.848556642102285	35.90447797962612	19.685634936978794
19	19.4362060636621	25.859576933218115	34.515682647949234	20.18853435517055
20	21.056723898019	25.470229239761583	34.972968915696505	18.50007794652291
21	20.338268663380724	25.934636547873602	36.424506386803365	17.302588401942312
22	20.037260362557184	22.208985213255914	37.04442181963752	20.70933260454938
23	19.198902205020527	24.588952379486013	36.55210773171769	19.66003768377577
24	19.606726111315336	23.953447642069566	36.56192321978802	19.877903026827077
25	20.20412365975284	26.56109563942131	33.77143769955753	19.463343001268317
26	19.876555802974284	24.823176869321212	34.61518475250536	20.68508257519915
27	21.579061831801027	24.022156058561897	33.86208661879531	20.536695490841765
28	19.39809887468316	25.172300307744422	34.60979585709419	20.819804960478226
29	21.38448421534796	24.162652260352935	33.75719561882803	20.695667905471076
30	21.561740382265146	25.41172123244038	33.53952273632712	19.487015648967354
31	21.63583769416864	24.894002351867925	31.79775475521905	21.67240519874439
32	21.97841747387829	26.18752971109747	32.049493155140524	19.784559659883715
33	22.39490210494104	26.094378804704505	30.836414305977634	20.674304784376822
34	23.180141150567664	25.84244794423263	31.198432601277553	19.778978303922155
35	22.380660024211537	26.481801892657053	31.651484736916053	19.48605334621536
36	22.207253068302325	28.43469909755248	30.276354104317466	19.081693729827727
37	21.206650666779577	28.329808097585197	30.85700758487029	19.606533650764934
38	20.898713786141684	27.29821954744826	30.830255568364873	20.972811098045177
39	22.37257668109479	26.497776118340145	30.20572108232115	20.923926118243912
40	22.911273761660702	25.202324153606614	32.37629116971748	19.510110915015193
41	20.92161659163913	26.93735601545073	31.460178949819763	20.680848443090376
42	21.39776399332547	26.818222934753948	31.881860015743275	19.902153056177312
43	21.74496282624469	26.344385059672394	30.93610887108415	20.974543242998767
44	21.534988365759727	26.086872843238957	30.37181453731521	22.006324253686103
45	21.69819491249781	24.51235308042734	30.157028563070288	23.632423444004566
46	22.77866844243601	25.28335004532446	29.751899104481062	22.18608240775847
47	20.838473633866897	25.60167979568388	32.323172057807454	21.23667451264177
48	20.682580588043965	25.053359687598036	31.81218929649895	22.45187042785905
49	20.971463874192388	24.46077365292049	32.63495814945331	21.932804323433803
50	19.76646836814624	24.317005621772676	33.98487644994967	21.93164956013141
51	20.110395371708684	23.472873647724057	32.309122437628346	24.10760854293891
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	155.0
1	343.5
2	532.0
3	9867.0
4	19202.0
5	12628.0
6	6054.0
7	5794.0
8	5534.0
9	5558.0
10	5582.0
11	5442.5
12	5303.0
13	5094.5
14	4886.0
15	4629.5
16	4373.0
17	4101.5
18	3830.0
19	3651.5
20	3473.0
21	3376.5
22	3280.0
23	3254.5
24	3229.0
25	3537.0
26	4321.5
27	4798.0
28	5633.5
29	6469.0
30	7408.5
31	8348.0
32	9156.5
33	9965.0
34	11693.5
35	13422.0
36	14302.0
37	15182.0
38	16675.0
39	18168.0
40	19417.0
41	20666.0
42	22672.0
43	24678.0
44	27847.5
45	31017.0
46	41209.0
47	51401.0
48	49516.0
49	47631.0
50	46453.5
51	45276.0
52	40450.5
53	35625.0
54	31593.5
55	27562.0
56	25036.0
57	22510.0
58	20373.5
59	18237.0
60	16886.0
61	15535.0
62	13509.5
63	11484.0
64	9795.0
65	8106.0
66	6633.5
67	5161.0
68	4302.5
69	3444.0
70	2817.5
71	2191.0
72	1835.0
73	1479.0
74	1206.5
75	747.0
76	560.0
77	394.0
78	228.0
79	170.0
80	112.0
81	105.5
82	99.0
83	56.0
84	13.0
85	8.5
86	4.0
87	3.0
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	519587.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.332144829903754
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.4805388457446	34.04510967980408
2	9.696553153120451	8.985242100824733
3	4.579128158050275	6.364824870404274
4	2.9175200526856684	5.4069984650072325
5	2.1276598048407673	4.92895211133236
6	1.5868781480838454	4.4114080910658116
7	1.2437368382586262	4.0337496724339665
8	1.0232049428263077	3.79258236813615
9	0.7479275010353754	3.11877767702151
>10	2.543850807412296	16.11496211335093
>50	0.03196930829011666	1.0309977668712862
>100	0.013460761377101092	1.1364362903293104
>500	0.003365190344275273	1.1676195876115416
>1k	0.0037858391373096823	3.324529281501866
>5k	0.0	0.0
>10k+	4.206487930344091E-4	2.1378099243049573
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10969	2.1110997773231435	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTC	4295	0.8266180639623394	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTT	2553	0.4913517851678352	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTC	2049	0.3943516677668995	No Hit
CGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTG	1708	0.32872262008094894	TruSeq Adapter, Index 21 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCT	1418	0.27290906046533114	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCT	1398	0.26905984945735745	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGC	1273	0.2450022806575222	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1249	0.24038322744795387	No Hit
GCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC	1115	0.21459351369453047	TruSeq Adapter, Index 15 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCG	1000	0.19246055039868204	No Hit
CGTCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCT	932	0.17937323297157165	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTT	915	0.17610140361479404	No Hit
CCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC	863	0.1660934549940626	TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTC	617	0.11874815959598681	No Hit
CTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGCT	599	0.11528386968881053	TruSeq Adapter, Index 21 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCC	549	0.10566084216887643	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.773816511960461E-4	0.0	0.0	0.12182752840236573	0.0
2	5.773816511960461E-4	0.0	0.0	0.5648717154201317	0.0
3	5.773816511960461E-4	0.0	0.0	1.0523742895799932	0.0
4	5.773816511960461E-4	0.0	0.0	1.7342620196425238	0.0
5	5.773816511960461E-4	0.0	0.0	3.6076730172232945	0.0
6	5.773816511960461E-4	0.0	0.0	5.404292255194991	0.0
7	5.773816511960461E-4	0.0	0.0	6.5627123080446585	0.0
8	5.773816511960461E-4	0.0	0.0	7.906471870928256	0.0
9	5.773816511960461E-4	0.0	0.0	8.683820033988534	0.0
10	5.773816511960461E-4	0.0	0.0	9.849938508854148	0.0
11	5.773816511960461E-4	0.0	0.0	10.754888016828751	0.0
12	5.773816511960461E-4	0.0	0.0	11.797831739439209	0.0
13	5.773816511960461E-4	0.0	0.0	12.323056581477212	0.0
14	5.773816511960461E-4	0.0	0.0	12.659862544674905	0.0
15	5.773816511960461E-4	0.0	0.0	12.981079203290305	0.0
16	5.773816511960461E-4	0.0	0.0	13.358109421521323	0.0
17	5.773816511960461E-4	0.0	0.0	13.779982947995235	0.0
18	5.773816511960461E-4	0.0	0.0	14.250356533169613	0.0
19	5.773816511960461E-4	0.0	0.0	14.631043501858207	0.0
20	5.773816511960461E-4	0.0	0.0	14.957071674233575	0.0
21	5.773816511960461E-4	0.0	0.0	15.344494762186121	0.0
22	5.773816511960461E-4	0.0	0.0	15.75366589233372	0.0
23	5.773816511960461E-4	0.0	0.0	16.14378342799185	0.0
24	5.773816511960461E-4	0.0	0.0	16.49791084072542	0.0
25	5.773816511960461E-4	0.0	0.0	16.826248539705574	0.0
26	5.773816511960461E-4	0.0	0.0	17.13957431575463	0.0
27	5.773816511960461E-4	0.0	0.0	17.499283084449765	0.0
28	5.773816511960461E-4	0.0	0.0	17.824926335724335	0.0
29	5.773816511960461E-4	0.0	0.0	18.173857313597146	0.0
30	5.773816511960461E-4	0.0	0.0	18.59245901071428	0.0
31	5.773816511960461E-4	0.0	0.0	18.981614243620413	0.0
32	5.773816511960461E-4	0.0	0.0	19.393287360923196	0.0
33	5.773816511960461E-4	0.0	0.0	19.77532155346458	0.0
34	5.773816511960461E-4	0.0	0.0	20.16216725976593	0.0
35	5.773816511960461E-4	0.0	0.0	20.544008991756915	0.0
36	5.773816511960461E-4	0.0	0.0	20.877543125597832	0.0
37	5.773816511960461E-4	0.0	0.0	21.253611041076855	0.0
38	5.773816511960461E-4	0.0	0.0	21.664129395077243	0.0
39	5.773816511960461E-4	0.0	0.0	22.153171653640296	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATTC	30	2.1631968E-6	45.000004	9
GCAAACG	20	7.029715E-4	45.0	1
CATACGA	20	7.029715E-4	45.0	18
ACATACG	20	7.029715E-4	45.0	17
GCGTTAG	50	2.1827873E-11	44.999996	1
TGCGAAG	50	2.1827873E-11	44.999996	1
CGTTTTT	5695	0.0	44.407375	1
CGTTATT	775	0.0	43.83871	1
TTACGGG	115	0.0	43.043476	3
AACACGT	105	0.0	42.857143	41
TCGCAAG	65	0.0	41.53846	1
ACGACCA	120	0.0	41.250004	28
ACACGAC	115	0.0	41.086956	26
GTTATTT	855	0.0	39.73684	2
CACGACC	125	0.0	39.6	27
CGTTTCT	315	0.0	39.285713	1
CAAACGG	35	6.241682E-6	38.571426	2
TTGGACG	35	6.241682E-6	38.571426	1
TAAACGG	35	6.241682E-6	38.571426	2
ACGGGCG	140	0.0	38.571426	5
>>END_MODULE