SRR2935820.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935820.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	386219
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	1065	0.2757502867544062	RNA PCR Primer, Index 33 (96% over 27bp)
CCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	984	0.2547777297336485	Illumina PCR Primer Index 5 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	925	0.23950142276791148	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTC	675	0.17477130850631376	Illumina PCR Primer Index 5 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGC	619	0.1602717629117159	No Hit
CTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGCT	617	0.15975392199762312	Illumina PCR Primer Index 5 (96% over 26bp)
GAATGATACCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCG	509	0.1317905126366129	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	20	7.027463E-4	45.0	1
ACCGGGT	20	7.027463E-4	45.0	4
CGCATGG	40	6.7939254E-9	45.0	2
TAGCGCG	25	3.8859795E-5	45.0	1
TAAACGG	35	1.2091186E-7	45.0	2
CGATTCG	20	7.027463E-4	45.0	10
TGTTGCG	20	7.027463E-4	45.0	1
TCGGACG	25	3.8859795E-5	45.0	1
TACGAGG	20	7.027463E-4	45.0	2
TACTGCG	25	3.8859795E-5	45.0	1
CGGGCTA	20	7.027463E-4	45.0	6
CATACGA	20	7.027463E-4	45.0	18
GCGATAT	20	7.027463E-4	45.0	9
CTTTCGA	20	7.027463E-4	45.0	23
CCGCGAA	25	3.8859795E-5	45.0	26
GCGTAAG	20	7.027463E-4	45.0	1
CGGCGCA	30	2.1617452E-6	44.999996	22
CGTAAGG	60	0.0	44.999996	2
ATAACGG	30	2.1617452E-6	44.999996	2
ACGGGCG	85	0.0	42.352943	5