##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935820.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	386219
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.117241772155175	33.0	31.0	34.0	30.0	34.0
2	32.20608773778608	33.0	31.0	34.0	30.0	34.0
3	32.141189325227394	33.0	31.0	34.0	30.0	34.0
4	35.76457916363514	37.0	35.0	37.0	35.0	37.0
5	32.3814105468659	37.0	35.0	37.0	16.0	37.0
6	34.034229284421535	37.0	35.0	37.0	19.0	37.0
7	35.55028105815612	37.0	35.0	37.0	32.0	37.0
8	35.78633366043618	37.0	35.0	37.0	35.0	37.0
9	37.70571618693021	39.0	37.0	39.0	35.0	39.0
10	37.32088012241759	39.0	37.0	39.0	34.0	39.0
11	37.32911379295167	39.0	37.0	39.0	34.0	39.0
12	37.26973297533265	39.0	37.0	39.0	34.0	39.0
13	37.21650151856848	39.0	37.0	39.0	34.0	39.0
14	38.42944288085258	40.0	38.0	41.0	34.0	41.0
15	38.581071878908084	40.0	38.0	41.0	35.0	41.0
16	38.534986626758396	40.0	38.0	41.0	34.0	41.0
17	38.51811278057268	40.0	38.0	41.0	34.0	41.0
18	38.43608936898495	40.0	38.0	41.0	34.0	41.0
19	38.335788762334325	40.0	37.0	41.0	34.0	41.0
20	38.41390247502065	40.0	37.0	41.0	34.0	41.0
21	38.34008943112586	40.0	37.0	41.0	34.0	41.0
22	38.40687019540727	40.0	37.0	41.0	34.0	41.0
23	38.429132176304115	40.0	37.0	41.0	34.0	41.0
24	38.40384082605983	40.0	37.0	41.0	34.0	41.0
25	38.124377619951375	40.0	37.0	41.0	34.0	41.0
26	38.185785784749065	40.0	37.0	41.0	34.0	41.0
27	38.16641594535743	40.0	37.0	41.0	34.0	41.0
28	38.030767517910824	40.0	37.0	41.0	34.0	41.0
29	37.900564187675904	40.0	36.0	41.0	33.0	41.0
30	37.783480356999526	40.0	36.0	41.0	33.0	41.0
31	37.78294180244887	40.0	36.0	41.0	33.0	41.0
32	37.716344871691966	40.0	36.0	41.0	33.0	41.0
33	37.675080718452485	40.0	36.0	41.0	33.0	41.0
34	37.60063849784708	40.0	36.0	41.0	33.0	41.0
35	37.59030239320178	40.0	36.0	41.0	33.0	41.0
36	37.515668571458164	40.0	36.0	41.0	33.0	41.0
37	37.44668439408729	40.0	36.0	41.0	33.0	41.0
38	37.283051843643115	40.0	35.0	41.0	33.0	41.0
39	37.19786960247942	40.0	35.0	41.0	32.0	41.0
40	37.159575266882264	40.0	35.0	41.0	32.0	41.0
41	37.062770086401756	39.0	35.0	41.0	32.0	41.0
42	37.081238882602875	39.0	35.0	41.0	32.0	41.0
43	36.98749673112923	39.0	35.0	41.0	32.0	41.0
44	36.926567569177074	39.0	35.0	41.0	32.0	41.0
45	36.892830233623926	39.0	35.0	41.0	32.0	41.0
46	36.76158604315168	39.0	35.0	41.0	31.0	41.0
47	36.718959968308134	39.0	35.0	41.0	31.0	41.0
48	36.66496728540025	39.0	35.0	41.0	31.0	41.0
49	36.558530263917625	39.0	35.0	41.0	31.0	41.0
50	36.437399506497606	39.0	35.0	41.0	31.0	41.0
51	35.37484691327977	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	7.0
9	16.0
10	16.0
11	9.0
12	8.0
13	10.0
14	14.0
15	19.0
16	30.0
17	39.0
18	74.0
19	148.0
20	241.0
21	452.0
22	695.0
23	1031.0
24	1345.0
25	1892.0
26	2450.0
27	3011.0
28	3426.0
29	3955.0
30	5064.0
31	6573.0
32	8710.0
33	12963.0
34	22509.0
35	30079.0
36	29657.0
37	46097.0
38	77811.0
39	127764.0
40	101.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.880026617022985	21.224745545920836	23.05375965449654	11.841468182559636
2	34.3874848207882	24.183688529046993	24.18291176767585	17.24591488248895
3	32.61776349687612	25.17147007267897	25.39828439305161	16.81248203739329
4	29.32740232873059	25.44255979120654	25.983185705519407	19.24685217454346
5	24.15650188105712	36.95701143651659	21.271869069103282	17.61461761332301
6	25.895411670580682	35.08449869115709	23.783397502453273	15.236692135808957
7	79.82103418008954	6.374362732025094	9.11503576986114	4.68956731802423
8	80.52400322097049	6.163601479989333	8.226679681734975	5.085715617305207
9	74.29101105849271	8.185252408607552	10.56343680657865	6.960299726321077
10	39.086373275266105	27.66150810809411	18.147993754838577	15.104124861801205
11	31.081329504762838	27.717176006359086	23.013368063197305	18.188126425680768
12	29.13088170183238	22.92688863054381	27.94839197450151	19.993837693122295
13	26.18307229835922	24.24764188193745	29.270439828180383	20.298845991522942
14	20.70250298405827	29.06718726939897	28.217669249829758	22.012640496713004
15	19.519754336270353	26.772893099510895	33.131979524570255	20.57537303964849
16	22.10274481576515	25.587555247152522	30.81438251354801	21.495317423534317
17	22.289944306209687	24.324541257680227	28.476848627333197	24.908665808776888
18	23.690962899287708	24.980127854921687	28.758036243685574	22.570873002105024
19	24.742438875353105	26.958280146756113	26.62297815488104	21.676302823009745
20	27.138229864403357	25.741612919095125	26.850310316167768	20.26984690033375
21	25.944865477876544	26.406520652790256	27.115185943726228	20.533427925606972
22	22.38522703440276	25.669891952493273	27.06624997734446	24.878631035759504
23	22.476108114826044	26.557730199705347	27.445050606003328	23.521111079465275
24	22.474813512540813	24.853515751426006	28.857979540105482	23.8136911959277
25	22.116726520445653	27.065214295516277	26.4463944031754	24.37166478086267
26	21.432399752472044	27.861653621390975	27.17240736473348	23.533539261403504
27	23.24121806539813	27.3254293548479	27.068321341000832	22.36503123875314
28	22.19077777116092	27.05692884089079	28.28369396637659	22.4685994215717
29	22.384709193488668	26.744411849235796	28.61226402636846	22.258614930907076
30	24.77635745522618	26.321076901964947	27.077901397911546	21.824664244897324
31	24.198964836012728	28.23061527268208	25.379383199687222	22.191036691617967
32	24.019274038822534	28.472705900020458	26.807588440755115	20.700431620401897
33	25.173023595421252	26.09400366113526	26.479536221677343	22.253436521766147
34	23.591537443781895	27.32309907073448	27.37980265082764	21.705560834655984
35	23.30491249783154	25.998462012485145	27.600920721145254	23.09570476853806
36	24.15857324471349	27.226003899342082	25.9837035464335	22.631719309510924
37	23.360839316553562	27.999917145453747	27.11207889824167	21.527164639751025
38	23.21325465603712	26.203527014465887	26.751143781119	23.832074548377992
39	24.005810175056123	24.637058249335222	27.6229289599942	23.734202615614457
40	24.959673138815024	24.522615407320718	29.5133072168899	21.00440423697436
41	20.886077588104158	26.17659928693306	28.53614141199682	24.40118171296596
42	23.26244954287593	26.068629456344716	27.676784415059853	22.9921365857195
43	23.657562160328727	25.32371530142225	27.60350992571572	23.415212612533303
44	23.322519088910695	25.095865299221426	27.5118520839213	24.06976352794658
45	23.253905167793402	24.61919273779902	26.758134633459257	25.368767460948323
46	23.021135676908695	26.227088776057105	27.191567478554912	23.560208068479284
47	22.02947032642102	25.992765762430125	29.29943891936958	22.678324991779274
48	22.369691806979976	24.113780005644468	28.942646529559653	24.573881657815903
49	23.007412892685238	23.922955628801276	29.47887079610273	23.590760682410757
50	21.132052022298232	24.63783501070636	29.591760115374953	24.638352851620454
51	21.32132287639914	24.460474497629583	26.86739906633283	27.350803559638443
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	157.0
1	412.0
2	667.0
3	1227.0
4	1787.0
5	1205.5
6	624.0
7	604.5
8	585.0
9	618.0
10	651.0
11	631.5
12	612.0
13	661.5
14	711.0
15	701.0
16	691.0
17	774.5
18	858.0
19	861.5
20	865.0
21	1260.5
22	1656.0
23	1588.5
24	1521.0
25	1812.0
26	2506.5
27	2910.0
28	3605.5
29	4301.0
30	5176.0
31	6051.0
32	6596.0
33	7141.0
34	8250.5
35	9360.0
36	10203.5
37	11047.0
38	12552.0
39	14057.0
40	15331.5
41	16606.0
42	18134.5
43	19663.0
44	22552.0
45	25441.0
46	30176.5
47	34912.0
48	36414.5
49	37917.0
50	37532.5
51	37148.0
52	32721.0
53	28294.0
54	25637.5
55	22981.0
56	21395.5
57	19810.0
58	19099.0
59	18388.0
60	17598.5
61	16809.0
62	15453.0
63	14097.0
64	11762.0
65	9427.0
66	7422.5
67	5418.0
68	4557.0
69	3696.0
70	3170.0
71	2644.0
72	2217.5
73	1791.0
74	1557.0
75	1062.0
76	801.0
77	593.0
78	385.0
79	304.5
80	224.0
81	131.0
82	38.0
83	27.0
84	16.0
85	18.0
86	20.0
87	13.5
88	7.0
89	5.0
90	3.0
91	2.5
92	2.0
93	1.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	386219.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.780169030638504
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.54398988704811	26.6818021020256
2	10.049266624723984	7.392274501826044
3	3.7349836913427343	4.121199944827918
4	2.037091320900649	2.9969825245429016
5	1.3645411336015953	2.509402677156288
6	1.0333462337602526	2.2803989486925467
7	0.9227438830483341	2.3757073193353824
8	0.7928306816696772	2.3328357187589663
9	0.7038509663595183	2.32989817635732
>10	6.7207771648448835	42.39275813001249
>50	0.06320204948765377	1.6188493963992552
>100	0.02840541550006911	1.5590371163451362
>500	0.004260812325010366	1.1306871631191313
>1k	7.101353875017277E-4	0.2781662806010337
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	1065	0.2757502867544062	RNA PCR Primer, Index 33 (96% over 27bp)
CCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	984	0.2547777297336485	Illumina PCR Primer Index 5 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	925	0.23950142276791148	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTC	675	0.17477130850631376	Illumina PCR Primer Index 5 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGC	619	0.1602717629117159	No Hit
CTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGCT	617	0.15975392199762312	Illumina PCR Primer Index 5 (96% over 26bp)
GAATGATACCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCG	509	0.1317905126366129	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.178409140927816E-4	0.0	0.0	0.16674477433787566	0.0
2	5.178409140927816E-4	0.0	0.0	0.8559910309953679	0.0
3	5.178409140927816E-4	0.0	0.0	1.1589279657396452	0.0
4	5.178409140927816E-4	0.0	0.0	1.5874413221514219	0.0
5	5.178409140927816E-4	0.0	0.0	2.847089345682113	0.0
6	5.178409140927816E-4	0.0	0.0	3.618154466766265	0.0
7	5.178409140927816E-4	0.0	0.0	4.3268197577022365	0.0
8	5.178409140927816E-4	0.0	0.0	5.374411926911933	0.0
9	5.178409140927816E-4	0.0	0.0	5.731722157635953	0.0
10	5.178409140927816E-4	0.0	0.0	6.76714506536447	0.0
11	5.178409140927816E-4	0.0	0.0	8.112754680634563	0.0
12	5.178409140927816E-4	0.0	0.0	9.344439294804243	0.0
13	7.767613711391723E-4	0.0	0.0	9.85192339061517	0.0
14	7.767613711391723E-4	0.0	0.0	10.084175040585782	0.0
15	7.767613711391723E-4	0.0	0.0	10.452101010048702	0.0
16	7.767613711391723E-4	0.0	0.0	11.172158801094715	0.0
17	7.767613711391723E-4	0.0	0.0	11.99190096810359	0.0
18	7.767613711391723E-4	0.0	0.0	12.965700807055065	0.0
19	7.767613711391723E-4	0.0	0.0	13.52988848295915	0.0
20	7.767613711391723E-4	0.0	0.0	14.05575593122037	0.0
21	7.767613711391723E-4	0.0	0.0	14.692959176011538	0.0
22	7.767613711391723E-4	0.0	0.0	15.408615319287762	0.0
23	7.767613711391723E-4	0.0	0.0	16.094754530460698	0.0
24	7.767613711391723E-4	0.0	0.0	16.652987035852714	0.0
25	7.767613711391723E-4	0.0	0.0	17.143641301955626	0.0
26	7.767613711391723E-4	0.0	0.0	17.605555397326388	0.0
27	7.767613711391723E-4	0.0	0.0	18.04287204927774	0.0
28	7.767613711391723E-4	0.0	0.0	18.497018530937112	0.0
29	7.767613711391723E-4	0.0	0.0	18.991038762981624	0.0
30	7.767613711391723E-4	0.0	0.0	19.55600320025685	0.0
31	0.0010356818281855632	0.0	0.0	20.04821098910204	0.0
32	0.0010356818281855632	0.0	0.0	20.549998834857945	0.0
33	0.0010356818281855632	0.0	0.0	21.046349351015873	0.0
34	0.0010356818281855632	0.0	0.0	21.555645890026124	0.0
35	0.0010356818281855632	0.0	0.0	22.04241634927334	0.0
36	0.0010356818281855632	0.0	0.0	22.53307061537625	0.0
37	0.0010356818281855632	0.0	0.0	23.065152154606583	0.0
38	0.0010356818281855632	0.0	0.0	23.536128465973967	0.0
39	0.0010356818281855632	0.0	0.0	24.048790970925822	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	20	7.027463E-4	45.0	1
ACCGGGT	20	7.027463E-4	45.0	4
CGCATGG	40	6.7939254E-9	45.0	2
TAGCGCG	25	3.8859795E-5	45.0	1
TAAACGG	35	1.2091186E-7	45.0	2
CGATTCG	20	7.027463E-4	45.0	10
TGTTGCG	20	7.027463E-4	45.0	1
TCGGACG	25	3.8859795E-5	45.0	1
TACGAGG	20	7.027463E-4	45.0	2
TACTGCG	25	3.8859795E-5	45.0	1
CGGGCTA	20	7.027463E-4	45.0	6
CATACGA	20	7.027463E-4	45.0	18
GCGATAT	20	7.027463E-4	45.0	9
CTTTCGA	20	7.027463E-4	45.0	23
CCGCGAA	25	3.8859795E-5	45.0	26
GCGTAAG	20	7.027463E-4	45.0	1
CGGCGCA	30	2.1617452E-6	44.999996	22
CGTAAGG	60	0.0	44.999996	2
ATAACGG	30	2.1617452E-6	44.999996	2
ACGGGCG	85	0.0	42.352943	5
>>END_MODULE