FastQCFastQC Report
Sat 14 Jan 2017
SRR2935818.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935818.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences612729
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132852.168168962134973No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTC44930.7332768646497881No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTT38970.6360071091787723No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTC31290.5106662162228326No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCT22390.3654144001671212No Hit
CGCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTG22290.36378235729009073TruSeq Adapter, Index 20 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCT15870.2590052045847348No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCG15560.2539458716659404No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15550.25378266737823735No Hit
GCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC15040.245459248705382TruSeq Adapter, Index 23 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCT14120.23044445423670168No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGC13710.2237530784408768No Hit
CCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC11320.18474725367984868TruSeq Adapter, Index 23 (95% over 22bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTC9760.15928738479817342No Hit
CTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGCT8720.14231413887705657TruSeq Adapter, Index 20 (95% over 23bp)
GAATGCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTT8240.13448033306731033No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCC8100.1321954730394677No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGT6920.11293736709050821No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAG253.8886654E-545.01
ACTACGG253.8886654E-545.02
CCGACGG253.8886654E-545.02
TCGACTG207.0307055E-445.01
TCGACGG207.0307055E-445.02
TAGTACG207.0307055E-445.01
AGTCGAC207.0307055E-445.015
CGTTTTT58300.043.5720441
CGTTATT7800.043.5576931
CTAGCGG750.042.02
CGTTTCT4050.041.111111
CGTAAGG950.040.263162
CGTTTTC2800.040.178571
CGGCGAA451.925764E-840.00000431
ACACGCG2850.039.47368636
TCACGAC403.4546974E-739.37525
GTTATTT8850.039.1525462
ACACGAC750.039.026
CGGGTCA356.243514E-638.5714266
TATGGGC1400.038.5714264
Produced by FastQC (version 0.11.5)