##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935817.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	443278
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08026565721737	33.0	31.0	34.0	30.0	34.0
2	32.17542264673636	33.0	31.0	34.0	30.0	34.0
3	32.143844269284735	33.0	31.0	34.0	30.0	34.0
4	35.76617156727832	37.0	35.0	37.0	35.0	37.0
5	31.93810430474781	37.0	35.0	37.0	0.0	37.0
6	33.792365062105496	37.0	35.0	37.0	17.0	37.0
7	35.50325078167651	37.0	35.0	37.0	32.0	37.0
8	35.75435279892077	37.0	35.0	37.0	35.0	37.0
9	37.705701613885644	39.0	37.0	39.0	35.0	39.0
10	37.26865984777047	39.0	37.0	39.0	34.0	39.0
11	37.29086036302275	39.0	37.0	39.0	34.0	39.0
12	37.24455082363664	39.0	37.0	39.0	34.0	39.0
13	37.18651500864018	39.0	37.0	39.0	34.0	39.0
14	38.39910846015367	40.0	38.0	41.0	34.0	41.0
15	38.53525327221292	40.0	38.0	41.0	34.0	41.0
16	38.48995438528418	40.0	38.0	41.0	34.0	41.0
17	38.47947563380091	40.0	38.0	41.0	34.0	41.0
18	38.4293287733657	40.0	38.0	41.0	34.0	41.0
19	38.30182864929006	40.0	37.0	41.0	34.0	41.0
20	38.38336438984114	40.0	37.0	41.0	34.0	41.0
21	38.32697314100858	40.0	37.0	41.0	34.0	41.0
22	38.404145930995895	40.0	37.0	41.0	34.0	41.0
23	38.394565035936814	40.0	37.0	41.0	34.0	41.0
24	38.3639950550219	40.0	37.0	41.0	34.0	41.0
25	38.11286596672968	40.0	37.0	41.0	34.0	41.0
26	38.153515401170374	40.0	37.0	41.0	34.0	41.0
27	38.15012700833337	40.0	37.0	41.0	34.0	41.0
28	38.08754100135806	40.0	37.0	41.0	34.0	41.0
29	38.05517530759478	40.0	37.0	41.0	34.0	41.0
30	37.931341054597794	40.0	36.0	41.0	34.0	41.0
31	37.934122604776235	40.0	37.0	41.0	34.0	41.0
32	37.88134308492639	40.0	37.0	41.0	34.0	41.0
33	37.844639255726655	40.0	37.0	41.0	34.0	41.0
34	37.75496189749999	40.0	36.0	41.0	33.0	41.0
35	37.76670396455498	40.0	37.0	41.0	33.0	41.0
36	37.68965525020416	40.0	36.0	41.0	33.0	41.0
37	37.64995781428359	40.0	36.0	41.0	33.0	41.0
38	37.533119171264985	40.0	36.0	41.0	33.0	41.0
39	37.4694232513231	40.0	36.0	41.0	33.0	41.0
40	37.39024043602435	40.0	36.0	41.0	33.0	41.0
41	37.307707578539876	40.0	35.0	41.0	33.0	41.0
42	37.31004020050623	40.0	35.0	41.0	33.0	41.0
43	37.258361569940305	40.0	35.0	41.0	33.0	41.0
44	37.21865511033708	40.0	35.0	41.0	33.0	41.0
45	37.206364854560796	39.0	35.0	41.0	33.0	41.0
46	37.07124648640357	39.0	35.0	41.0	32.0	41.0
47	36.9921020217561	39.0	35.0	41.0	32.0	41.0
48	36.97314326449767	39.0	35.0	41.0	32.0	41.0
49	36.93010255415338	39.0	35.0	41.0	32.0	41.0
50	36.83518243630408	39.0	35.0	41.0	32.0	41.0
51	35.81133058712591	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	5.0
9	15.0
10	18.0
11	17.0
12	18.0
13	7.0
14	12.0
15	15.0
16	18.0
17	45.0
18	74.0
19	178.0
20	296.0
21	482.0
22	771.0
23	1109.0
24	1485.0
25	2088.0
26	2646.0
27	3006.0
28	3567.0
29	4240.0
30	5438.0
31	7398.0
32	9979.0
33	14671.0
34	25237.0
35	33730.0
36	32952.0
37	51938.0
38	88425.0
39	153268.0
40	127.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.27655331417304	20.14153646244569	23.741083473576403	10.840826749804863
2	32.243422863304744	25.262701961297424	24.81332256507203	17.6805526103258
3	32.639111347732126	25.315490504829924	25.219613876619185	16.825784270818765
4	29.09370643253218	25.214876443225243	26.624826858089058	19.06659026615352
5	23.63528079444502	37.33932205072212	20.982318093837275	18.04307906099558
6	25.5683792112399	36.030662473662126	23.62625711178989	14.774701203308084
7	80.14677019838567	5.706351319036812	9.496523626257112	4.650354856320413
8	80.78429337797049	6.440427903031506	7.72517472105541	5.050103997942601
9	74.99041233717892	6.936279264930811	10.09659852282315	7.9767098750671135
10	39.118566678247056	25.477014424356724	19.010643433691722	16.393775463704493
11	30.873853428322633	26.10731865781744	22.829917117474814	20.188910796385112
12	27.889270390138922	22.45746461588439	28.239163685091523	21.41410130888517
13	25.083356268526746	23.584522579509922	30.632921101430703	20.699200050532625
14	21.004877300475098	28.118923113711936	28.323309525850593	22.552890059962373
15	20.167028365946425	26.3696822310153	33.197000527885436	20.26628887515284
16	22.18743091242967	24.92092998073444	30.472525142235796	22.419113964600093
17	21.851975509725275	25.47949593708688	28.30751808120412	24.36101047198372
18	22.68057516953244	25.473179359228293	29.266509955377888	22.57973551586138
19	23.681527168052554	27.45184737343157	26.750707231128096	22.11591822738778
20	25.464606860705924	25.97173782592414	28.223597832511427	20.340057480858516
21	24.366650273643174	27.130604270909004	27.88250262814757	20.62024282730025
22	22.078695536435376	25.017257793077935	27.881825851948438	25.022220818538255
23	21.76534815623604	27.13692084876759	28.18299126056335	22.91473973443302
24	22.882480068940936	25.072753441406974	28.60101335956217	23.44375313008992
25	21.384323156123244	28.35895307233835	26.415703012556452	23.841020758981948
26	20.227035855603027	28.204873691002035	27.673153190548593	23.89493726284634
27	22.684861418793623	27.19625156222506	28.070646411507	22.048240607474316
28	19.764120935394942	27.44553079557298	29.733485532780783	23.05686273625129
29	21.089925509499682	26.587604167136654	28.174418762040975	24.148051561322692
30	22.358204106677977	26.13799917884488	29.331931654627567	22.171865059849573
31	21.604275420842	27.16624781739676	27.85272447538565	23.376752286375595
32	22.280600435843873	27.358452257950994	26.9142614792523	23.446685826952837
33	23.29125289321825	25.234954137132902	27.26031970907647	24.21347326057237
34	20.793497534278714	25.55055743799602	29.424424401842636	24.23152062588263
35	20.647539467332013	24.83001637798402	28.0054503043237	26.516993850360272
36	21.891002937208704	26.738976443676428	28.11080179932232	23.259218819792547
37	20.48037574614576	25.973542562455165	30.45560573725743	23.090475954141645
38	21.525994973808764	25.56273940958044	27.061121914464515	25.850143702146283
39	22.99911116725847	23.997581653048424	27.93957742094126	25.063729758751847
40	22.804650806040453	23.864256741818902	29.819210518004503	23.511881934136138
41	19.77607731491299	25.543112899805543	28.546420079498645	26.13438970578283
42	20.469547326959606	25.32519096368419	28.35128294208149	25.85397876727471
43	21.69270751086226	24.5579523459319	28.41715582546393	25.332184317741913
44	21.485162809794307	24.921381164867192	27.945893998799846	25.64756202653865
45	21.553066021774146	23.70972617634983	27.62059023908247	27.11661756279355
46	22.51047875148327	25.52822382342458	27.799259155654017	24.162038269438142
47	19.905115976881323	25.18667743492797	30.643298336484104	24.264908251706604
48	20.388108590997074	24.14353971999513	29.104083667585577	26.36426802142222
49	21.092181430163464	23.06453286650815	30.810236465603978	25.03304923772441
50	20.674836107363774	23.4915786481621	29.784920523915016	26.048664720559106
51	20.42848957087877	23.076940430158952	27.87483249789072	28.619737501071562
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	168.0
1	408.0
2	648.0
3	1101.5
4	1555.0
5	1067.5
6	580.0
7	580.0
8	580.0
9	613.5
10	647.0
11	626.5
12	606.0
13	665.5
14	725.0
15	711.5
16	698.0
17	655.5
18	613.0
19	702.0
20	791.0
21	943.0
22	1095.0
23	1357.0
24	1619.0
25	1819.5
26	2711.5
27	3403.0
28	3848.0
29	4293.0
30	5664.5
31	7036.0
32	7709.5
33	8383.0
34	9367.5
35	10352.0
36	11239.5
37	12127.0
38	13787.5
39	15448.0
40	17684.0
41	19920.0
42	22430.0
43	24940.0
44	28029.5
45	31119.0
46	37849.5
47	44580.0
48	45909.0
49	47238.0
50	46008.5
51	44779.0
52	39486.5
53	34194.0
54	30050.5
55	25907.0
56	24033.0
57	22159.0
58	20874.5
59	19590.0
60	18536.5
61	17483.0
62	16082.5
63	14682.0
64	11688.0
65	8694.0
66	6921.5
67	5149.0
68	4518.5
69	3888.0
70	3030.0
71	2172.0
72	1805.0
73	1438.0
74	1208.0
75	780.5
76	583.0
77	389.0
78	195.0
79	147.5
80	100.0
81	76.5
82	53.0
83	37.5
84	22.0
85	22.0
86	22.0
87	11.5
88	1.0
89	0.5
90	0.0
91	1.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	443278.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.13175730598929
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.51650197987158	25.12500390930151
2	10.440952941951545	7.336180495997932
3	4.13869909856595	4.361993168800068
4	2.199105287466177	3.090337329983182
5	1.4140192418646187	2.4838490415593375
6	1.0530875627598049	2.219809000609994
7	0.8476271966988542	2.084504307226614
8	0.7490499296332536	2.105235227035506
9	0.6024025839550196	1.904711524200773
>10	6.937481406436528	43.95684466618812
>50	0.0667339448209236	1.6129117106254853
>100	0.02980350933749986	1.8234597394610166
>500	0.0019437071307065125	0.5230695894746493
>1k	0.0025916095076086837	1.3720902895357645
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGC	1620	0.3654591475326996	TruSeq Adapter, Index 22 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTC	1536	0.34650941395692997	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGC	1510	0.3406440202310965	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCG	1362	0.3072563944071215	No Hit
CCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGC	912	0.20573996453692717	TruSeq Adapter, Index 22 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	722	0.16287747192506735	No Hit
CTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGCT	664	0.14979313207513117	TruSeq Adapter, Index 20 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.767761991346288E-4	0.0	0.0	0.15543293373458641	0.0
2	6.767761991346288E-4	0.0	0.0	0.9204156308230952	0.0
3	6.767761991346288E-4	0.0	0.0	1.2152644615794153	0.0
4	6.767761991346288E-4	0.0	0.0	1.6517851100212508	0.0
5	6.767761991346288E-4	0.0	0.0	3.097604663439196	0.0
6	6.767761991346288E-4	0.0	0.0	3.886500119563795	0.0
7	6.767761991346288E-4	0.0	0.0	4.6600553151746755	0.0
8	6.767761991346288E-4	0.0	0.0	5.85027905738611	0.0
9	6.767761991346288E-4	0.0	0.0	6.246193133879868	0.0
10	6.767761991346288E-4	0.0	0.0	7.542219555222682	0.0
11	6.767761991346288E-4	0.0	0.0	8.972022071927775	0.0
12	6.767761991346288E-4	0.0	0.0	10.41039708715524	0.0
13	6.767761991346288E-4	0.0	0.0	10.88030536142105	0.0
14	6.767761991346288E-4	0.0	0.0	11.075216906771823	0.0
15	6.767761991346288E-4	0.0	0.0	11.47496604839401	0.0
16	6.767761991346288E-4	0.0	0.0	12.195958292538768	0.0
17	6.767761991346288E-4	0.0	0.0	13.064487748094875	0.0
18	6.767761991346288E-4	0.0	0.0	13.956253186487938	0.0
19	6.767761991346288E-4	0.0	0.0	14.56963801497029	0.0
20	6.767761991346288E-4	0.0	0.0	15.142641863570942	0.0
21	6.767761991346288E-4	0.0	0.0	15.821448391302974	0.0
22	6.767761991346288E-4	0.0	0.0	16.59071733765267	0.0
23	6.767761991346288E-4	0.0	0.0	17.346901944152428	0.0
24	6.767761991346288E-4	0.0	0.0	17.944495327988307	0.0
25	6.767761991346288E-4	0.0	0.0	18.489074576225303	0.0
26	6.767761991346288E-4	0.0	0.0	18.966201796615216	0.0
27	6.767761991346288E-4	0.0	0.0	19.449420002797343	0.0
28	6.767761991346288E-4	0.0	0.0	19.922937750125204	0.0
29	6.767761991346288E-4	0.0	0.0	20.454207066445885	0.0
30	6.767761991346288E-4	0.0	0.0	21.0400696628301	0.0
31	6.767761991346288E-4	0.0	0.0	21.57404608394732	0.0
32	6.767761991346288E-4	0.0	0.0	22.101029151006816	0.0
33	6.767761991346288E-4	0.0	0.0	22.60973926068968	0.0
34	6.767761991346288E-4	0.0	0.0	23.119351738638056	0.0
35	6.767761991346288E-4	0.0	0.0	23.656260856618193	0.0
36	9.023682655128385E-4	0.0	0.0	24.15324017884939	0.0
37	9.023682655128385E-4	0.0	0.0	24.65856640753658	0.0
38	0.001127960331891048	0.0	0.0	25.189384539724507	0.0
39	0.001127960331891048	0.0	0.0	25.690875703283268	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTG	20	7.028591E-4	45.000004	1
AGGTACG	20	7.028591E-4	45.000004	1
GTATTCG	20	7.028591E-4	45.000004	22
GATCGGA	20	7.028591E-4	45.000004	15
GATCGCA	20	7.028591E-4	45.000004	10
ATCCGAT	20	7.028591E-4	45.000004	29
CTAACGG	20	7.028591E-4	45.000004	2
TGCGACG	20	7.028591E-4	45.000004	1
TGCGAAT	20	7.028591E-4	45.000004	27
TATTCGG	20	7.028591E-4	45.000004	23
TTGCGTG	20	7.028591E-4	45.000004	1
CTACCTT	45	3.8380676E-10	45.000004	17
CGAGACA	20	7.028591E-4	45.000004	22
ATCGGAA	20	7.028591E-4	45.000004	16
TTACCGG	35	1.2096643E-7	45.000004	2
TGTCGTA	20	7.028591E-4	45.000004	37
TCAACGG	40	6.7993824E-9	45.000004	2
ATCGAAC	20	7.028591E-4	45.000004	39
TGCAACG	20	7.028591E-4	45.000004	1
CCCGATG	20	7.028591E-4	45.000004	31
>>END_MODULE