FastQCFastQC Report
Sat 14 Jan 2017
SRR2935815.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935815.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences529282
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20470.3867503523641461No Hit
GAATCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTC15880.3000290960206468No Hit
GCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGC14760.278868353732037TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGC10670.2015938573388099No Hit
CCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGC10530.19894876455273372TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCG10380.19611473656765202No Hit
CTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGCT8550.16153959514965557Illumina Single End Adapter 1 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG8330.15738302077153576No Hit
CGCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTG6860.12960954651773535No Hit
GAATGCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTT5340.10089139626890768No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGC207.0298323E-445.00000412
TCGATGT207.0298323E-445.00000413
ACGATAG351.2102646E-745.0000041
GTTAAGC207.0298323E-445.00000417
GCGTATT207.0298323E-445.0000041
TCGTAAG502.1827873E-1145.01
CGTAAGG1100.045.02
TCACGAC302.1632732E-644.99999625
GTACGAG302.1632732E-644.9999961
CTACGAA700.041.78571711
CGAATAT700.041.78571714
TACGGGA2900.040.3448264
ATAGCGG451.9248546E-840.0000042
GCGTAAG451.9248546E-840.0000041
CGTTTTT8700.039.8275831
CACGACC2000.039.37527
CGAAGTA356.241902E-638.571433
ACGGGAT2050.038.4146355
CTACGGG1600.037.9687543
CGTTATT1250.037.8000031
Produced by FastQC (version 0.11.5)