##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935815.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	529282
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.049070249885695	33.0	31.0	34.0	30.0	34.0
2	32.159347946841194	33.0	31.0	34.0	30.0	34.0
3	32.105465517436826	33.0	31.0	34.0	30.0	34.0
4	35.74659633238992	37.0	35.0	37.0	33.0	37.0
5	32.02711597976126	37.0	35.0	37.0	0.0	37.0
6	33.827810127682405	37.0	35.0	37.0	17.0	37.0
7	35.475555563952675	37.0	35.0	37.0	32.0	37.0
8	35.72890066165107	37.0	35.0	37.0	35.0	37.0
9	37.66705083490464	39.0	37.0	39.0	35.0	39.0
10	37.29750870046591	39.0	37.0	39.0	34.0	39.0
11	37.28205946924324	39.0	37.0	39.0	34.0	39.0
12	37.16885894475913	39.0	37.0	39.0	34.0	39.0
13	37.09392346612959	39.0	37.0	39.0	33.0	39.0
14	38.16390695319319	40.0	38.0	41.0	33.0	41.0
15	38.35310855082923	40.0	38.0	41.0	34.0	41.0
16	38.33001311210281	40.0	37.0	41.0	34.0	41.0
17	38.32716774800579	40.0	37.0	41.0	34.0	41.0
18	38.256167789571535	40.0	37.0	41.0	34.0	41.0
19	38.13019524563465	40.0	37.0	41.0	34.0	41.0
20	38.20898122361992	40.0	37.0	41.0	34.0	41.0
21	38.112633718887096	40.0	37.0	41.0	34.0	41.0
22	38.203107228282846	40.0	37.0	41.0	34.0	41.0
23	38.219342429933384	40.0	37.0	41.0	34.0	41.0
24	38.19793418253408	40.0	37.0	41.0	34.0	41.0
25	37.9188258810993	40.0	36.0	41.0	34.0	41.0
26	37.98159771161687	40.0	36.0	41.0	34.0	41.0
27	37.961746290257366	40.0	36.0	41.0	34.0	41.0
28	37.86184113572727	40.0	36.0	41.0	34.0	41.0
29	37.809600553202266	40.0	36.0	41.0	33.0	41.0
30	37.65125207356381	40.0	36.0	41.0	33.0	41.0
31	37.636165597923224	40.0	36.0	41.0	33.0	41.0
32	37.58842356248654	40.0	36.0	41.0	33.0	41.0
33	37.5175331864677	40.0	36.0	41.0	33.0	41.0
34	37.39859847869378	40.0	35.0	41.0	33.0	41.0
35	37.370105161331765	40.0	35.0	41.0	33.0	41.0
36	37.280455409403686	40.0	35.0	41.0	33.0	41.0
37	37.213540229971926	40.0	35.0	41.0	32.0	41.0
38	37.08953639080868	40.0	35.0	41.0	32.0	41.0
39	36.99475326952362	39.0	35.0	41.0	32.0	41.0
40	36.90373751610672	39.0	35.0	41.0	31.0	41.0
41	36.770131990130025	39.0	35.0	41.0	31.0	41.0
42	36.77497062057655	39.0	35.0	41.0	31.0	41.0
43	36.716378036660984	39.0	35.0	41.0	31.0	41.0
44	36.68205795776165	39.0	35.0	41.0	31.0	41.0
45	36.63754104617198	39.0	35.0	41.0	31.0	41.0
46	36.52170676501373	39.0	35.0	41.0	31.0	41.0
47	36.43958419141403	39.0	35.0	41.0	31.0	41.0
48	36.41324662467267	39.0	35.0	41.0	31.0	41.0
49	36.36015583375214	39.0	35.0	41.0	31.0	41.0
50	36.24492425587872	38.0	35.0	41.0	31.0	41.0
51	35.23071443956152	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	17.0
9	28.0
10	20.0
11	26.0
12	24.0
13	18.0
14	18.0
15	26.0
16	43.0
17	77.0
18	127.0
19	232.0
20	415.0
21	697.0
22	1039.0
23	1533.0
24	2237.0
25	3133.0
26	3918.0
27	4526.0
28	5137.0
29	5990.0
30	7325.0
31	9653.0
32	13093.0
33	20041.0
34	34327.0
35	40946.0
36	42080.0
37	65037.0
38	103040.0
39	164357.0
40	102.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.500886106083335	19.995389981144267	22.593437902668143	11.910286010104254
2	33.753840107919785	23.687939510506688	23.923730638865482	18.634489742708045
3	32.56298154858847	24.224704410881156	26.116701493721685	17.095612546808695
4	28.97699147146512	25.328085973072955	25.974244353671576	19.72067820179035
5	23.29344281498332	36.395720995612926	22.21556750465725	18.095268684746504
6	25.58749400130743	33.722287929685876	24.90430432170374	15.78591374730295
7	79.11661458353014	5.835641491681183	10.420531965946319	4.627211958842356
8	79.5972657298	5.945412842303347	9.367973972286984	5.089347455609675
9	74.04540490702499	6.799777812205969	11.68148548410866	7.473331796660382
10	40.550217086543654	24.710834677922165	19.129311028903306	15.60963720663087
11	31.158059408783977	24.766003756031758	24.223570799687124	19.852366035497145
12	27.14110814272921	22.307578946572903	29.897861631417655	20.653451279280233
13	26.180183720587515	23.210500262619927	31.479816052690246	19.129499964102312
14	21.461338190227515	26.425799479294593	31.82896830045231	20.283894030025582
15	20.50967159283709	24.45482748326979	34.23789208777174	20.797608836121388
16	24.351290994214807	25.16201193314717	29.180663615992984	21.30603345664504
17	23.798844472322884	24.452938131279733	28.05649918191059	23.691718214486794
18	25.765282023571558	23.15438650851531	29.65035652072052	21.429974947192612
19	24.446325399314542	25.70274447270075	27.299246904296766	22.55168322368794
20	25.37588657842133	26.59659689919551	27.475523444968847	20.551993077414306
21	24.66964680453898	26.782887005414885	29.153079076938194	19.394387113107946
22	23.372795598565606	23.29495429657536	29.899184177810696	23.43306592704834
23	21.519719166720197	26.273328773697198	29.510922343854507	22.6960297157281
24	22.634814711250336	24.934533953544612	28.978314017858153	23.452337317346895
25	22.909148620206242	27.161702079420802	26.88434520728081	23.04480409309215
26	21.498936294829598	25.445414731655337	28.190076367607436	24.865572605907627
27	22.643316795205582	25.157288553172037	28.63709704845432	23.562297603168066
28	20.388375195075593	26.181695202179554	28.735532287136156	24.694397315608693
29	21.73246020080033	25.69499812954153	29.200690747087567	23.371850922570577
30	22.56453081722031	25.5757800189691	28.890081279922615	22.969607883887985
31	22.793898148812918	26.590173102429326	26.357593872453627	24.258334876304126
32	22.77349314732033	27.252768845341425	26.62342569745429	23.350312309883954
33	22.344043439980954	25.880154624566863	25.17108082269943	26.60472111275275
34	20.304865837115184	25.318828148321685	28.8307556274349	25.545550387128223
35	19.89317603848232	29.074293098952918	27.077248045465367	23.95528281709939
36	21.88209687841264	27.511232197580878	25.76887179235266	24.837799131653824
37	20.13557989880631	28.10392191686096	26.13087163364709	25.629626550685646
38	20.75509841634516	28.07577057220914	25.281230043719603	25.88790096772609
39	21.800665807641295	24.60238587369304	25.900937496457466	27.6960108222082
40	22.88402023873852	24.240197097199605	27.600220676312436	25.27556198774944
41	19.762999686367568	25.379665282401444	26.719593713748058	28.137741317482927
42	21.247274609754346	25.30371333240125	28.342736008403836	25.106276049440563
43	21.519341296322185	24.792832554290527	27.912152689870428	25.775673459516856
44	21.575455050426804	24.437445444961288	27.283376347580308	26.7037231570316
45	21.039256955649353	23.869695171949925	26.81349450765376	28.27755336474696
46	22.185337872816383	24.65925536859368	26.817462146832877	26.33794461175706
47	19.481675175048462	26.00541866150748	29.501853454302246	25.01105270914182
48	20.729592164479428	26.229306872328927	27.470044324197683	25.571056638993955
49	22.448146734632957	23.65997710105388	29.05653319024641	24.835342974066755
50	20.566918958135737	24.086970650806187	29.017612539251285	26.328497851806787
51	20.205296987239315	23.752177478168537	26.825019554793094	29.217505979799046
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	167.0
1	401.5
2	636.0
3	2112.0
4	3588.0
5	2317.0
6	1046.0
7	1031.5
8	1017.0
9	1045.0
10	1073.0
11	1062.0
12	1051.0
13	1043.5
14	1036.0
15	1057.0
16	1078.0
17	1127.5
18	1177.0
19	1163.0
20	1149.0
21	1379.0
22	1609.0
23	1617.5
24	1626.0
25	2055.0
26	2954.5
27	3425.0
28	4122.0
29	4819.0
30	6037.0
31	7255.0
32	8099.0
33	8943.0
34	10880.5
35	12818.0
36	13606.0
37	14394.0
38	16239.5
39	18085.0
40	19482.0
41	20879.0
42	23407.5
43	25936.0
44	29173.5
45	32411.0
46	40562.0
47	48713.0
48	50748.0
49	52783.0
50	53283.5
51	53784.0
52	47984.5
53	42185.0
54	38189.5
55	34194.0
56	31244.5
57	28295.0
58	26576.5
59	24858.0
60	22352.0
61	19846.0
62	17711.5
63	15577.0
64	14011.0
65	12445.0
66	10586.5
67	8728.0
68	8076.0
69	7424.0
70	5966.0
71	4508.0
72	3913.5
73	3319.0
74	2777.5
75	1841.5
76	1447.0
77	1050.0
78	653.0
79	492.0
80	331.0
81	216.5
82	102.0
83	86.0
84	70.0
85	51.5
86	33.0
87	30.0
88	27.0
89	18.5
90	10.0
91	8.0
92	6.0
93	3.0
94	0.0
95	1.0
96	2.0
97	3.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	529282.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.4914749291281
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.0357053620992	26.286891362231014
2	10.475762155846603	7.645520241471707
3	3.970065106420881	4.346205938938916
4	2.2311573285084263	3.2567288686482274
5	1.3697975222287893	2.499296597019683
6	1.059936714415663	2.3207192424336953
7	0.883632728150943	2.2571543082114047
8	0.7479334113772278	2.1834554663943493
9	0.6600592181496753	2.16778809697639
>10	6.468026456400327	41.26386849170832
>50	0.0605444063777707	1.5115828418021684
>100	0.03158838593622233	2.0141630037432243
>500	0.002632365494685194	0.6580034612572055
>1k	0.0031588385936222327	1.588622079163747
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2047	0.3867503523641461	No Hit
GAATCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTC	1588	0.3000290960206468	No Hit
GCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGC	1476	0.278868353732037	TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGC	1067	0.2015938573388099	No Hit
CCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGC	1053	0.19894876455273372	TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCG	1038	0.19611473656765202	No Hit
CTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGCT	855	0.16153959514965557	Illumina Single End Adapter 1 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	833	0.15738302077153576	No Hit
CGCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTG	686	0.12960954651773535	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTT	534	0.10089139626890768	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16890806791086793	0.0
2	0.0	0.0	0.0	0.7969286694049675	0.0
3	1.8893519900544512E-4	0.0	0.0	1.160439992291444	0.0
4	1.8893519900544512E-4	0.0	0.0	1.6170963682876047	0.0
5	1.8893519900544512E-4	0.0	0.0	3.0240967952811544	0.0
6	1.8893519900544512E-4	0.0	0.0	4.038678813940395	0.0
7	1.8893519900544512E-4	0.0	0.0	4.90456883098235	0.0
8	1.8893519900544512E-4	0.0	0.0	6.169112117925794	0.0
9	1.8893519900544512E-4	0.0	0.0	6.711356139071421	0.0
10	1.8893519900544512E-4	0.0	0.0	7.918085255119199	0.0
11	1.8893519900544512E-4	0.0	0.0	9.269538733605149	0.0
12	1.8893519900544512E-4	0.0	0.0	10.563744846792448	0.0
13	1.8893519900544512E-4	0.0	0.0	11.084828125649464	0.0
14	1.8893519900544512E-4	0.0	0.0	11.355383330625262	0.0
15	1.8893519900544512E-4	0.0	0.0	11.755925952516806	0.0
16	1.8893519900544512E-4	0.0	0.0	12.41984424182194	0.0
17	1.8893519900544512E-4	0.0	0.0	13.232076662346348	0.0
18	1.8893519900544512E-4	0.0	0.0	14.074916585109639	0.0
19	1.8893519900544512E-4	0.0	0.0	14.615460189464217	0.0
20	1.8893519900544512E-4	0.0	0.0	15.160160368196916	0.0
21	1.8893519900544512E-4	0.0	0.0	15.781946108123835	0.0
22	1.8893519900544512E-4	0.0	0.0	16.44246356384687	0.0
23	1.8893519900544512E-4	0.0	0.0	17.0784194436992	0.0
24	1.8893519900544512E-4	0.0	0.0	17.61480647367566	0.0
25	1.8893519900544512E-4	0.0	0.0	18.092434656761423	0.0
26	1.8893519900544512E-4	0.0	0.0	18.564016913479016	0.0
27	1.8893519900544512E-4	0.0	0.0	19.100403943455472	0.0
28	1.8893519900544512E-4	0.0	0.0	19.559516477038706	0.0
29	1.8893519900544512E-4	0.0	0.0	20.0694525791544	0.0
30	1.8893519900544512E-4	0.0	0.0	20.753775869952126	0.0
31	3.7787039801089024E-4	0.0	0.0	21.244818452167276	0.0
32	5.668055970163353E-4	0.0	0.0	21.731893395203315	0.0
33	5.668055970163353E-4	0.0	0.0	22.255621766846406	0.0
34	5.668055970163353E-4	0.0	0.0	22.80976870552938	0.0
35	5.668055970163353E-4	0.0	0.0	23.386587868093002	0.0
36	5.668055970163353E-4	0.0	0.0	23.84003234570607	0.0
37	5.668055970163353E-4	0.0	0.0	24.36432752294618	0.0
38	5.668055970163353E-4	0.0	0.0	24.908838766479874	0.0
39	5.668055970163353E-4	0.0	0.0	25.70123299110871	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGGC	20	7.0298323E-4	45.000004	12
TCGATGT	20	7.0298323E-4	45.000004	13
ACGATAG	35	1.2102646E-7	45.000004	1
GTTAAGC	20	7.0298323E-4	45.000004	17
GCGTATT	20	7.0298323E-4	45.000004	1
TCGTAAG	50	2.1827873E-11	45.0	1
CGTAAGG	110	0.0	45.0	2
TCACGAC	30	2.1632732E-6	44.999996	25
GTACGAG	30	2.1632732E-6	44.999996	1
CTACGAA	70	0.0	41.785717	11
CGAATAT	70	0.0	41.785717	14
TACGGGA	290	0.0	40.344826	4
ATAGCGG	45	1.9248546E-8	40.000004	2
GCGTAAG	45	1.9248546E-8	40.000004	1
CGTTTTT	870	0.0	39.827583	1
CACGACC	200	0.0	39.375	27
CGAAGTA	35	6.241902E-6	38.57143	3
ACGGGAT	205	0.0	38.414635	5
CTACGGG	160	0.0	37.968754	3
CGTTATT	125	0.0	37.800003	1
>>END_MODULE