FastQCFastQC Report
Sat 14 Jan 2017
SRR2935811.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935811.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386477
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21390.5534611374027433No Hit
GAATCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTTC8780.22718040142104184No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGC6390.1653397226743118No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCG6070.15705979916010526No Hit
GCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTTCTGC5930.1534373326226399No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGG406.7957444E-945.0000042
TTAAGCG207.027468E-445.0000041
TCGTAAG207.027468E-445.0000041
TTACGCG207.027468E-445.0000041
TAGTGCG207.027468E-445.0000041
GGTCATA253.8859842E-545.08
ACCGGTA302.1617489E-644.99999641
CGAAAGG1100.042.9545442
CGGGCAA603.6379788E-1241.2499966
TAGGGTC603.6379788E-1241.2499965
GACGGGA1600.040.7812544
CGTTATT950.040.2631571
CGATAGG451.9226718E-840.02
TATGCGG403.4502773E-739.3750042
ATGGGCG1100.038.8636365
CGTTTTT9350.038.7433171
TACGCGG356.237533E-638.5714262
GTACGAG356.237533E-638.5714261
GACGGGT301.1385203E-437.4999964
GCGATAT301.1385203E-437.4999969