##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935810.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	545573
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.010953621238585	33.0	31.0	34.0	30.0	34.0
2	32.116510531129656	33.0	31.0	34.0	30.0	34.0
3	32.0805318445011	33.0	31.0	34.0	30.0	34.0
4	35.71330692684572	37.0	35.0	37.0	33.0	37.0
5	31.956706068665422	37.0	35.0	37.0	0.0	37.0
6	33.779617026502414	37.0	35.0	37.0	17.0	37.0
7	35.47517380808801	37.0	35.0	37.0	32.0	37.0
8	35.74428353309273	37.0	35.0	37.0	35.0	37.0
9	37.6939163045092	39.0	37.0	39.0	35.0	39.0
10	37.28555298740957	39.0	37.0	39.0	34.0	39.0
11	37.216498983637386	39.0	37.0	39.0	34.0	39.0
12	37.03935312048067	39.0	37.0	39.0	34.0	39.0
13	36.90198745172507	39.0	37.0	39.0	33.0	39.0
14	37.825196628132254	40.0	37.0	41.0	33.0	41.0
15	38.052900345141715	40.0	37.0	41.0	33.0	41.0
16	38.09652603776213	40.0	37.0	41.0	33.0	41.0
17	38.12776292081903	40.0	37.0	41.0	33.0	41.0
18	38.08993663542734	40.0	37.0	41.0	34.0	41.0
19	37.95300720526859	40.0	36.0	41.0	33.0	41.0
20	38.01950426432393	40.0	36.0	41.0	33.0	41.0
21	37.93873047236575	40.0	36.0	41.0	33.0	41.0
22	38.05510903215519	40.0	36.0	41.0	34.0	41.0
23	38.08416288929254	40.0	36.0	41.0	34.0	41.0
24	38.051600794027564	40.0	36.0	41.0	34.0	41.0
25	37.79029937331943	40.0	35.0	41.0	33.0	41.0
26	37.84316122682024	40.0	36.0	41.0	34.0	41.0
27	37.81623907341456	40.0	35.0	41.0	34.0	41.0
28	37.7234650541724	40.0	35.0	41.0	33.0	41.0
29	37.68654240587419	40.0	35.0	41.0	33.0	41.0
30	37.50882466690984	40.0	35.0	41.0	33.0	41.0
31	37.44811418453626	40.0	35.0	41.0	33.0	41.0
32	37.38999730558514	40.0	35.0	41.0	33.0	41.0
33	37.35763866613634	40.0	35.0	41.0	33.0	41.0
34	37.22154872033624	40.0	35.0	41.0	32.0	41.0
35	37.21208527548101	40.0	35.0	41.0	32.0	41.0
36	37.098216004091114	39.0	35.0	41.0	32.0	41.0
37	37.00598270075682	39.0	35.0	41.0	32.0	41.0
38	36.850428815208964	39.0	35.0	41.0	31.0	41.0
39	36.77428501777031	39.0	35.0	41.0	31.0	41.0
40	36.68736905968587	39.0	35.0	41.0	31.0	41.0
41	36.54011103921932	39.0	35.0	41.0	31.0	41.0
42	36.5416085473438	39.0	35.0	41.0	31.0	41.0
43	36.47484021386689	39.0	35.0	41.0	31.0	41.0
44	36.414364347209265	39.0	35.0	41.0	31.0	41.0
45	36.391725763555016	38.0	35.0	41.0	31.0	41.0
46	36.30720728481798	38.0	35.0	40.0	31.0	41.0
47	36.19741812736334	38.0	35.0	40.0	31.0	41.0
48	36.13049582732283	38.0	35.0	40.0	31.0	41.0
49	36.0718144043052	38.0	35.0	40.0	31.0	41.0
50	35.927247132830985	38.0	35.0	40.0	30.0	41.0
51	34.863127390834954	36.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	13.0
9	38.0
10	20.0
11	14.0
12	10.0
13	19.0
14	14.0
15	29.0
16	33.0
17	77.0
18	144.0
19	253.0
20	488.0
21	769.0
22	1136.0
23	1668.0
24	2467.0
25	3091.0
26	4079.0
27	4726.0
28	5612.0
29	6747.0
30	8253.0
31	11077.0
32	15181.0
33	23059.0
34	40008.0
35	44144.0
36	43359.0
37	67676.0
38	106727.0
39	154537.0
40	100.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.05561492229271	19.62743757480667	23.480634122289775	10.836313380610845
2	33.94889409849828	23.649447461659577	24.210875538195623	18.190782901646525
3	33.236432154817045	24.200061220038382	25.11377945756113	17.44972716758344
4	28.8643316293145	25.173166560661908	25.880129698500475	20.082372111523114
5	23.252800266875376	36.7069118156507	22.10446631339894	17.93582160407498
6	25.734411343669866	34.255544170990866	24.219673627543887	15.790370857795383
7	80.2257443091942	5.470945226395001	9.62565962758421	4.6776508368266025
8	81.03113607161644	5.258508027340063	8.682614425567248	5.027741475476242
9	75.80103854113015	6.072881172638675	10.892217906677933	7.233862379553241
10	42.423470369684715	23.62543600947994	18.254385755893345	15.696707864941997
11	33.43988797099563	24.5226578294747	22.869533499641662	19.167920699888008
12	29.314317240772546	21.89844438782711	28.356425262980387	20.430813108419954
13	27.369206320694023	22.649764559463172	30.708447815416086	19.272581304426723
14	21.83080907596234	25.817809898950276	31.305434836401364	21.045946188686024
15	21.098551431247515	23.514543424986208	34.198539883755245	21.188365260011036
16	26.146455194813527	23.825042661568663	29.428325815243788	20.60017632837402
17	25.61307102807507	23.237220316987827	27.76658668959058	23.383121965346525
18	27.643046851658713	22.098417626972008	29.297454236188376	20.961081285180903
19	25.59712449113134	25.690787483984728	26.326266145868654	22.385821879015275
20	25.772169810456163	27.32484928689653	26.938833116741478	19.96414778590583
21	24.531822505879138	26.925635982719086	29.382135846165408	19.160405665236365
22	22.938268572674968	22.98867429289939	29.97802310598215	24.09503402844349
23	22.219391355510627	26.528255613822534	28.987688173718272	22.264664856948567
24	23.17508381096572	25.886361678455494	28.322332666755866	22.616221843822917
25	23.922554818511912	26.156902925914586	26.49251337584521	23.428028879728284
26	23.118629404314365	25.056591876797423	27.05962355175201	24.765155167136204
27	23.925854102017514	24.042795372938176	28.012200017229596	24.019150507814718
28	20.97977722504596	26.628700467215204	27.62068504123188	24.770837266506955
29	24.140674116937603	26.39701744771094	26.31343559890244	23.148872836449016
30	26.52037399211471	24.57489648498001	27.358025415480604	21.54670410742467
31	26.230403630678207	25.748891532388885	25.779684845107802	22.24101999182511
32	26.94249898730326	26.897408779393405	24.409015842059635	21.7510763912437
33	26.682772058001405	24.58277810668783	24.434860229520154	24.299589605790608
34	22.83470772930479	26.330298603486607	28.81649201848332	22.018501648725287
35	23.685372993164986	28.113194751206528	26.106130618633987	22.0953016369945
36	27.015633105010693	27.894342278668482	23.83475721855737	21.255267397763454
37	23.399801676402607	28.792663859831773	25.942449498050674	21.865084965714946
38	23.74329374804105	29.372054702120522	23.87673143648971	23.007920113348717
39	23.82522595509675	24.920588078955518	25.10754747760611	26.146638488341615
40	25.473034772615215	23.409516233391315	28.295388518126813	22.822060475866657
41	23.17490051743763	24.91417280547241	26.246900048206196	25.66402662888376
42	24.665810074912066	24.585160922552983	27.469651174086696	23.279377828448254
43	24.641248742148164	24.357876947722854	27.065488944650852	23.93538536547813
44	24.30857098866696	23.863900889523492	27.290023516559653	24.537504605249893
45	22.87576547959668	23.78801736889472	26.33469764816074	27.001519503347854
46	23.969294668174562	24.6914711688445	26.935167246179702	24.404066916801234
47	21.26608171592069	26.142972617779836	29.263178346435765	23.327767319863703
48	22.39590302306016	27.16776673332441	26.859283725550938	23.577046518064492
49	24.697886442327608	24.33496525671175	28.205757982891384	22.76139031806926
50	22.327717830611117	24.59377571837316	27.715814382309972	25.362692068705744
51	22.14130831254479	24.424412498419095	25.56358177549109	27.870697413545027
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	208.0
1	506.5
2	805.0
3	1637.0
4	2469.0
5	1651.5
6	834.0
7	832.0
8	830.0
9	836.5
10	843.0
11	879.5
12	916.0
13	870.5
14	825.0
15	846.5
16	868.0
17	857.0
18	846.0
19	934.5
20	1023.0
21	1153.0
22	1283.0
23	1383.5
24	1484.0
25	1795.5
26	2372.5
27	2638.0
28	3644.5
29	4651.0
30	5329.5
31	6008.0
32	6925.0
33	7842.0
34	9026.5
35	10211.0
36	11393.0
37	12575.0
38	14268.0
39	15961.0
40	17676.0
41	19391.0
42	22335.0
43	25279.0
44	28998.5
45	32718.0
46	41020.5
47	49323.0
48	52330.0
49	55337.0
50	56852.5
51	58368.0
52	53372.5
53	48377.0
54	43556.0
55	38735.0
56	35853.5
57	32972.0
58	30729.0
59	28486.0
60	25568.5
61	22651.0
62	19974.0
63	17297.0
64	14649.5
65	12002.0
66	10660.5
67	9319.0
68	8107.0
69	6895.0
70	5932.0
71	4969.0
72	4295.0
73	3621.0
74	2956.0
75	1822.5
76	1354.0
77	938.0
78	522.0
79	397.5
80	273.0
81	195.0
82	117.0
83	68.5
84	20.0
85	17.0
86	14.0
87	10.0
88	6.0
89	5.0
90	4.0
91	2.5
92	1.0
93	2.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	545573.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.94004158461883
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.36540740927161	24.93510302583215
2	10.926184660547369	7.6352269280149905
3	4.277074852592452	4.483235196303232
4	2.3579503697641466	3.2954753589610655
5	1.4591384265761531	2.549117865114304
6	0.9984170411452171	2.0930839761843587
7	0.7721087082674943	1.888425726229881
8	0.6543907194328308	1.8291551159657395
9	0.567986429273444	1.7860922512481965
>10	6.504173526496427	43.458840594620426
>50	0.0756265256448618	1.8280900122501604
>100	0.03674810048939965	2.2369167383201263
>500	0.0015977434995391154	0.41961960277114085
>1k	0.0031954869990782307	1.561617608184219
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	1759	0.32241331590822864	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTC	1595	0.29235317730166266	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGC	1416	0.2595436357737645	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCG	1299	0.2380982929873729	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1270	0.23278278067279723	No Hit
CCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	1053	0.193008085077524	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	888	0.16276465294286924	No Hit
CTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGCT	837	0.1534166830103396	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.498805842664501E-4	0.0	0.0	0.15946536943727055	0.0
2	5.498805842664501E-4	0.0	0.0	0.8486490350512214	0.0
3	5.498805842664501E-4	0.0	0.0	1.1596981522179433	0.0
4	5.498805842664501E-4	0.0	0.0	1.590071356170485	0.0
5	5.498805842664501E-4	0.0	0.0	2.9215155442076495	0.0
6	5.498805842664501E-4	0.0	0.0	3.7826285391689103	0.0
7	5.498805842664501E-4	0.0	0.0	4.61606421138876	0.0
8	5.498805842664501E-4	0.0	0.0	5.979401473313379	0.0
9	5.498805842664501E-4	0.0	0.0	6.547428116860622	0.0
10	5.498805842664501E-4	0.0	0.0	7.900317647684178	0.0
11	5.498805842664501E-4	0.0	0.0	9.349436280754363	0.0
12	5.498805842664501E-4	0.0	0.0	10.72377115436431	0.0
13	5.498805842664501E-4	0.0	0.0	11.247990644698326	0.0
14	5.498805842664501E-4	0.0	0.0	11.494887027033963	0.0
15	5.498805842664501E-4	0.0	0.0	11.89025116712154	0.0
16	5.498805842664501E-4	0.0	0.0	12.560555599342344	0.0
17	5.498805842664501E-4	0.0	0.0	13.342852377225412	0.0
18	5.498805842664501E-4	0.0	0.0	14.196450337535031	0.0
19	5.498805842664501E-4	0.0	0.0	14.760077936408143	0.0
20	7.331741123552669E-4	0.0	0.0	15.30281007307913	0.0
21	7.331741123552669E-4	0.0	0.0	15.943970834333811	0.0
22	7.331741123552669E-4	0.0	0.0	16.625089584711855	0.0
23	7.331741123552669E-4	0.0	0.0	17.28586275347204	0.0
24	7.331741123552669E-4	0.0	0.0	17.82016338785094	0.0
25	7.331741123552669E-4	0.0	0.0	18.294710332072885	0.0
26	7.331741123552669E-4	0.0	0.0	18.767424341013943	0.0
27	7.331741123552669E-4	0.0	0.0	19.30355791067373	0.0
28	7.331741123552669E-4	0.0	0.0	19.786536357187764	0.0
29	7.331741123552669E-4	0.0	0.0	20.321020285094754	0.0
30	7.331741123552669E-4	0.0	0.0	21.013136647158124	0.0
31	7.331741123552669E-4	0.0	0.0	21.515177620593395	0.0
32	7.331741123552669E-4	0.0	0.0	22.028949379826347	0.0
33	7.331741123552669E-4	0.0	0.0	22.55720132777832	0.0
34	9.164676404440835E-4	0.0	0.0	23.105798857348145	0.0
35	9.164676404440835E-4	0.0	0.0	23.706451748895198	0.0
36	0.0010997611685329002	0.0	0.0	24.207392961161933	0.0
37	0.001283054696621717	0.0	0.0	24.73472844147346	0.0
38	0.001283054696621717	0.0	0.0	25.366357939267523	0.0
39	0.001283054696621717	0.0	0.0	26.390052293643564	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	30	2.1633969E-6	45.000004	4
GAATCGC	30	2.1633969E-6	45.000004	17
CTATGCG	20	7.0300244E-4	45.0	1
TAGCCCG	20	7.0300244E-4	45.0	1
TCTCGTT	20	7.0300244E-4	45.0	20
TATTCGG	50	2.1827873E-11	45.0	1
TGTTACG	25	3.8881015E-5	45.0	1
TTAACGG	20	7.0300244E-4	45.0	2
CGAAAGG	90	0.0	42.5	2
GGCGATT	170	0.0	41.02941	8
AACACGT	205	0.0	40.60976	41
CACGACC	220	0.0	39.886364	27
AAACACG	210	0.0	39.642857	40
CGTTTTT	655	0.0	39.503815	1
TACGAAT	40	3.4537698E-7	39.375	12
GCGATTA	40	3.4537698E-7	39.375	9
TAACGGG	155	0.0	39.193546	3
AACGGGC	115	0.0	39.130432	4
ACGACCA	220	0.0	38.863636	28
TCCGACG	35	6.242255E-6	38.571426	26
>>END_MODULE