FastQCFastQC Report
Sat 14 Jan 2017
SRR2935809.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935809.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences755110
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147151.9487227026525937No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTC62540.8282237025069195No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTT30340.40179576485545154No Hit
CGCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTG23140.306445418548291No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTC21800.2886996596522361No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCG21120.27969434916767094No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGC21070.2790321939849823No Hit
GAACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCT20760.27492683185231287No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCT20180.267245831733125No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16670.22076253790838418No Hit
GCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC16090.21308153778919628TruSeq Adapter, Index 15 (95% over 23bp)
CGTCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCT15020.19891141687965994No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTT12220.16183072664909748No Hit
CCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC12100.16024155421064482TruSeq Adapter, Index 15 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGCT9450.12514732952814822TruSeq Adapter, Index 14 (95% over 22bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTC9110.12064467428586564No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCC8980.11892307081087523No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGT8110.10740157063209334No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT253.8895305E-545.028
TTCCGCG253.8895305E-545.01
CCGACGG253.8895305E-545.02
CGTTTTT88200.044.2857131
CGTTATT9800.042.9336741
GCTACGA603.6379788E-1241.25000410
CGTTACT451.9268555E-840.01
GCTAGCG451.9268555E-840.01
TGTAACG403.4561162E-739.3751
GTTATTT10900.038.6009182
GTATGCG356.2454437E-638.5714261
CGAATAT659.094947E-1238.07692714
TACGGGA3200.037.968754
GACGTAG301.1395509E-437.5000041
AAGCGCG301.1395509E-437.5000041
TACGAAG601.546141E-1037.5000041
GTTTTTT104900.037.3856052
CGAAAGG850.037.0588232
GTTACGG850.037.0588232
GGCCGAT800.036.56258
Produced by FastQC (version 0.11.5)