##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935809.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	755110
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.9864099270305	33.0	31.0	34.0	30.0	34.0
2	32.15710029002397	33.0	31.0	34.0	30.0	34.0
3	31.95478274688456	33.0	31.0	34.0	30.0	34.0
4	35.69418098025453	37.0	35.0	37.0	33.0	37.0
5	32.06654792017057	37.0	35.0	37.0	0.0	37.0
6	33.85557865741415	37.0	35.0	37.0	17.0	37.0
7	35.49868363549682	37.0	35.0	37.0	32.0	37.0
8	35.75503171723325	37.0	35.0	37.0	35.0	37.0
9	37.72734700904504	39.0	38.0	39.0	35.0	39.0
10	37.40384182436996	39.0	37.0	39.0	34.0	39.0
11	37.3541921044616	39.0	37.0	39.0	34.0	39.0
12	37.411483095178184	39.0	37.0	39.0	35.0	39.0
13	37.44203890823854	39.0	37.0	39.0	35.0	39.0
14	38.75079392406404	40.0	38.0	41.0	35.0	41.0
15	38.83693501609037	40.0	38.0	41.0	35.0	41.0
16	38.760571307491624	40.0	38.0	41.0	35.0	41.0
17	38.722455006555336	40.0	38.0	41.0	35.0	41.0
18	38.46626186913165	40.0	38.0	41.0	34.0	41.0
19	38.18073790573559	40.0	37.0	41.0	34.0	41.0
20	38.04794268384739	40.0	36.0	41.0	34.0	41.0
21	37.97815285190237	40.0	36.0	41.0	34.0	41.0
22	38.02615380540583	40.0	35.0	41.0	34.0	41.0
23	38.01583080610772	40.0	35.0	41.0	34.0	41.0
24	37.97372568235092	40.0	35.0	41.0	34.0	41.0
25	37.72247884414191	40.0	35.0	41.0	33.0	41.0
26	37.75437221067129	40.0	35.0	41.0	34.0	41.0
27	37.71635523301241	40.0	35.0	41.0	34.0	41.0
28	37.6154374859292	40.0	35.0	41.0	33.0	41.0
29	37.53183774549404	40.0	35.0	41.0	33.0	41.0
30	37.23774416972362	40.0	35.0	41.0	33.0	41.0
31	37.03829110990452	39.0	35.0	41.0	32.0	41.0
32	36.672222590086214	39.0	35.0	41.0	31.0	41.0
33	36.24253022738409	40.0	35.0	41.0	29.0	41.0
34	35.85733072002755	40.0	35.0	41.0	25.0	41.0
35	35.62211465879143	40.0	35.0	41.0	23.0	41.0
36	35.43078094582246	39.0	35.0	41.0	22.0	41.0
37	35.35072903285614	39.0	35.0	41.0	21.0	41.0
38	35.2118154970799	39.0	35.0	41.0	21.0	41.0
39	35.114019149527884	39.0	35.0	41.0	20.0	41.0
40	35.043168544980205	39.0	35.0	41.0	20.0	41.0
41	34.904725139383665	39.0	35.0	41.0	18.0	41.0
42	34.865904305333	39.0	35.0	41.0	18.0	41.0
43	34.81103680258505	39.0	35.0	41.0	18.0	41.0
44	34.77076452437393	39.0	35.0	41.0	18.0	41.0
45	34.764358835136605	39.0	35.0	41.0	18.0	41.0
46	34.62610613023268	39.0	35.0	41.0	18.0	41.0
47	34.51652077180808	39.0	34.0	41.0	18.0	41.0
48	34.44844989471733	39.0	34.0	41.0	18.0	41.0
49	34.40524162042616	39.0	34.0	41.0	18.0	41.0
50	34.319778575306906	38.0	34.0	41.0	18.0	41.0
51	33.30730886890652	37.0	32.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	9.0
9	23.0
10	30.0
11	17.0
12	16.0
13	18.0
14	15.0
15	36.0
16	74.0
17	117.0
18	259.0
19	632.0
20	1054.0
21	1798.0
22	2796.0
23	4493.0
24	7230.0
25	12305.0
26	16842.0
27	18158.0
28	16117.0
29	14133.0
30	14000.0
31	15072.0
32	17946.0
33	23270.0
34	36025.0
35	46824.0
36	52967.0
37	79762.0
38	137232.0
39	235659.0
40	179.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.624703685555744	17.39163830435301	21.007667757015533	22.97599025307571
2	41.77974070003046	22.312245897948642	21.150560845439735	14.75745255658116
3	27.56499053118089	22.80396233661321	35.18864801154798	14.442399120657917
4	23.74488485121373	22.11161287759399	37.39640582166837	16.74709644952391
5	21.27531088185827	33.204433791103284	30.301545470196395	15.21870985684205
6	22.220338758591463	30.437949437830248	33.57497583133583	13.766735972242456
7	70.06701010448809	4.452596310471322	21.119836844962986	4.360556740077605
8	71.28762696825628	4.925772404020607	19.366317490166995	4.420283137556118
9	65.38292434214883	6.111162612069765	21.108845068930354	7.397067976851054
10	31.513951609699248	27.749202103004862	27.431367615314322	13.305478671981566
11	22.243116896875954	22.203917310060785	38.14027095390075	17.41269483916251
12	20.26115400405239	20.065023638940023	40.19083312365086	19.48298923335673
13	20.368158281574868	20.68400630371734	41.83152123531671	17.116314179391082
14	17.494802081815894	24.52424150123823	39.334401610361404	18.64655480658447
15	16.71173736276834	24.57946524347446	41.21147912224709	17.497318271510114
16	19.769437565387822	24.922196766034087	37.47983737468713	17.828528293890955
17	19.639787580617394	24.878891817086252	36.959780694203495	18.52153990809286
18	19.687462753770973	24.220312272384156	37.23431023294619	18.857914740898675
19	18.620333461350004	26.239090993365206	35.83981141820397	19.30076412708082
20	19.720570512905404	25.66010250162228	36.386089443922074	18.23323754155024
21	19.83035584219518	26.043622783435527	37.14518414535631	16.98083722901299
22	19.33983128285945	22.772443749917233	37.36263590735125	20.52508905987207
23	18.26687502483082	24.790427884679055	37.47189151249487	19.47080557799526
24	18.586298684959807	23.990676855027747	37.64497887724968	19.778045582762775
25	19.206340798029427	26.60645468872085	35.170107666432706	19.01709684681702
26	19.186608573585307	24.860219040934435	35.87702453947107	20.07614784600919
27	20.2734700904504	24.108143184436702	35.72419912330654	19.894187601806358
28	19.2729536094079	24.483055448875	36.54924448093656	19.69474646078055
29	21.17903351829535	22.936260942114394	35.47403689528678	20.410668644303477
30	21.301532227092743	23.26972229211638	36.133808319317716	19.294937161473165
31	22.293308259723748	23.062467719934844	34.83254095429805	19.811683066043358
32	22.693779714213825	23.89665081908596	33.83877845611898	19.57079101058124
33	21.142614983247473	25.23314483982466	32.95956880454503	20.66467137238283
34	22.2948974321622	25.593357259207266	32.780522043146036	19.3312232654845
35	21.111228827588032	25.57071155195932	33.57153262438585	19.746526996066798
36	21.303518692640807	27.896200553561734	31.902106977791316	18.898173776006143
37	20.66758485518666	27.169419025042707	32.77774099137874	19.38525512839189
38	20.534756525539326	26.929321555799817	32.515395107997506	20.02052681066335
39	21.531697368595303	26.426613341102623	31.023691912436597	21.017997377865477
40	21.483757333368647	25.351538186489385	33.45155010528267	19.713154374859293
41	20.12912026062428	26.903894796784574	32.53380302207625	20.433181920514894
42	20.767437856736105	26.38781104739707	33.19198527366874	19.65276582219809
43	21.050575412853757	25.89649190184212	32.39938551999047	20.65354716531366
44	20.946219756062032	25.868019228986505	31.384036762855743	21.801724252095724
45	20.653414734277124	24.40094820622161	31.600296645521848	23.34534041397942
46	21.870191097985725	25.096608441154267	30.95164942856008	22.081551032299927
47	20.52442690468938	24.880480989524706	33.41049648395598	21.18459562182993
48	20.420733403080344	24.505303863013335	32.900901855358825	22.173060878547496
49	20.345909867436532	23.91201281932434	33.9480340612626	21.794043251976532
50	19.729178530280358	23.69323674696402	34.67680205532969	21.90078266742594
51	19.593304286792655	23.184436704586087	33.32110553429302	23.901153474328243
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	233.0
1	463.0
2	693.0
3	13691.0
4	26689.0
5	17890.5
6	9092.0
7	8997.5
8	8903.0
9	8710.5
10	8518.0
11	8359.5
12	8201.0
13	7850.5
14	7500.0
15	7236.5
16	6973.0
17	6489.0
18	6005.0
19	5728.0
20	5451.0
21	5220.0
22	4989.0
23	5186.5
24	5384.0
25	5911.0
26	7053.5
27	7669.0
28	8921.5
29	10174.0
30	11623.0
31	13072.0
32	15172.0
33	17272.0
34	18797.5
35	20323.0
36	22940.5
37	25558.0
38	27597.5
39	29637.0
40	31264.0
41	32891.0
42	35591.0
43	38291.0
44	42617.5
45	46944.0
46	61857.5
47	76771.0
48	73273.0
49	69775.0
50	68197.5
51	66620.0
52	58001.0
53	49382.0
54	43521.5
55	37661.0
56	33410.5
57	29160.0
58	26297.0
59	23434.0
60	20730.5
61	18027.0
62	15558.0
63	13089.0
64	10990.5
65	8892.0
66	7328.5
67	5765.0
68	4690.0
69	3615.0
70	2964.5
71	2314.0
72	2001.5
73	1689.0
74	1340.0
75	755.5
76	520.0
77	404.5
78	289.0
79	200.5
80	112.0
81	83.5
82	55.0
83	42.0
84	29.0
85	20.5
86	12.0
87	9.5
88	7.0
89	3.5
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	755110.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.54719787875118
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.94401320324108	31.46110561707289
2	9.516511635195169	8.098018073161736
3	4.116538775097535	5.254415695188804
4	2.4663143951794066	4.19739066411643
5	1.8142152159420495	3.859488689366384
6	1.3531702830279015	3.4544162277380317
7	1.1168730326131922	3.3263872546824085
8	0.9177023283687221	3.123653004711577
9	0.7346237270445539	2.8130562972891298
>10	3.96109792808134	25.5661277599307
>50	0.03573990984989506	1.0218918567107194
>100	0.016302397982101403	1.1459425081270112
>500	0.0025080612280037624	0.8063348226757425
>1k	0.003762091842005643	3.0747434239038114
>5k	3.135076535004703E-4	0.8342130655110161
>10k+	3.135076535004703E-4	1.962815039813656
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14715	1.9487227026525937	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTC	6254	0.8282237025069195	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTT	3034	0.40179576485545154	No Hit
CGCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTG	2314	0.306445418548291	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTC	2180	0.2886996596522361	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCG	2112	0.27969434916767094	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGC	2107	0.2790321939849823	No Hit
GAACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCT	2076	0.27492683185231287	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCT	2018	0.267245831733125	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1667	0.22076253790838418	No Hit
GCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC	1609	0.21308153778919628	TruSeq Adapter, Index 15 (95% over 23bp)
CGTCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCT	1502	0.19891141687965994	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTT	1222	0.16183072664909748	No Hit
CCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC	1210	0.16024155421064482	TruSeq Adapter, Index 15 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGCT	945	0.12514732952814822	TruSeq Adapter, Index 14 (95% over 22bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTC	911	0.12064467428586564	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCC	898	0.11892307081087523	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGT	811	0.10740157063209334	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.6486207307544596E-4	0.0	0.0	0.13084186409927032	0.0
2	2.6486207307544596E-4	0.0	0.0	0.573161526135265	0.0
3	2.6486207307544596E-4	0.0	0.0	1.0525618784018222	0.0
4	2.6486207307544596E-4	0.0	0.0	1.7682191998516772	0.0
5	2.6486207307544596E-4	0.0	0.0	3.528095244401478	0.0
6	2.6486207307544596E-4	0.0	0.0	5.28439565096476	0.0
7	2.6486207307544596E-4	0.0	0.0	6.50289361814835	0.0
8	2.6486207307544596E-4	0.0	0.0	7.95685396829601	0.0
9	2.6486207307544596E-4	0.0	0.0	8.807326084941266	0.0
10	2.6486207307544596E-4	0.0	0.0	10.129782415806968	0.0
11	2.6486207307544596E-4	0.0	0.0	11.108712637893817	0.0
12	2.6486207307544596E-4	0.0	0.0	12.228019758710651	0.0
13	2.6486207307544596E-4	0.0	0.0	12.783965250096012	0.0
14	2.6486207307544596E-4	0.0	0.0	13.121664393267206	0.0
15	2.6486207307544596E-4	0.0	0.0	13.43963131199428	0.0
16	2.6486207307544596E-4	0.0	0.0	13.842354094105495	0.0
17	2.6486207307544596E-4	0.0	0.0	14.298446583941413	0.0
18	2.6486207307544596E-4	0.0	0.0	14.776390194806055	0.0
19	2.6486207307544596E-4	0.0	0.0	15.186264252890307	0.0
20	2.6486207307544596E-4	0.0	0.0	15.539325396299876	0.0
21	2.6486207307544596E-4	0.0	0.0	15.92880507475732	0.0
22	3.972931096131689E-4	0.0	0.0	16.374567943743294	0.0
23	3.972931096131689E-4	0.0	0.0	16.799009415846697	0.0
24	3.972931096131689E-4	0.0	0.0	17.156705645535087	0.0
25	3.972931096131689E-4	0.0	0.0	17.500099323277404	0.0
26	3.972931096131689E-4	0.0	0.0	17.833428242242853	0.0
27	3.972931096131689E-4	0.0	0.0	18.192051489187005	0.0
28	3.972931096131689E-4	0.0	0.0	18.550012580948472	0.0
29	3.972931096131689E-4	0.0	0.0	18.91896544874257	0.0
30	3.972931096131689E-4	0.0	0.0	19.379825455893844	0.0
31	3.972931096131689E-4	0.0	0.0	19.782415806968523	0.0
32	3.972931096131689E-4	0.0	0.0	20.181298089020142	0.0
33	3.972931096131689E-4	0.0	0.0	20.578193905523698	0.0
34	3.972931096131689E-4	0.0	0.0	20.955887221729284	0.0
35	3.972931096131689E-4	0.0	0.0	21.332918382752183	0.0
36	3.972931096131689E-4	0.0	0.0	21.71511435420005	0.0
37	5.297241461508919E-4	0.0	0.0	22.106845360278633	0.0
38	6.621551826886149E-4	0.0	0.0	22.510362728609078	0.0
39	6.621551826886149E-4	0.0	0.0	22.930698838579808	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGT	25	3.8895305E-5	45.0	28
TTCCGCG	25	3.8895305E-5	45.0	1
CCGACGG	25	3.8895305E-5	45.0	2
CGTTTTT	8820	0.0	44.285713	1
CGTTATT	980	0.0	42.933674	1
GCTACGA	60	3.6379788E-12	41.250004	10
CGTTACT	45	1.9268555E-8	40.0	1
GCTAGCG	45	1.9268555E-8	40.0	1
TGTAACG	40	3.4561162E-7	39.375	1
GTTATTT	1090	0.0	38.600918	2
GTATGCG	35	6.2454437E-6	38.571426	1
CGAATAT	65	9.094947E-12	38.076927	14
TACGGGA	320	0.0	37.96875	4
GACGTAG	30	1.1395509E-4	37.500004	1
AAGCGCG	30	1.1395509E-4	37.500004	1
TACGAAG	60	1.546141E-10	37.500004	1
GTTTTTT	10490	0.0	37.385605	2
CGAAAGG	85	0.0	37.058823	2
GTTACGG	85	0.0	37.058823	2
GGCCGAT	80	0.0	36.5625	8
>>END_MODULE