##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935808.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	982575
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.002240032567485	33.0	31.0	34.0	30.0	34.0
2	32.162321451288705	33.0	31.0	34.0	30.0	34.0
3	31.98044220542961	33.0	31.0	34.0	30.0	34.0
4	35.70950716230313	37.0	35.0	37.0	33.0	37.0
5	32.065217413429004	37.0	35.0	37.0	0.0	37.0
6	33.85390428211587	37.0	35.0	37.0	17.0	37.0
7	35.50368368826807	37.0	35.0	37.0	32.0	37.0
8	35.76628450754395	37.0	35.0	37.0	35.0	37.0
9	37.722057858178765	39.0	37.0	39.0	35.0	39.0
10	37.384701422283285	39.0	37.0	39.0	34.0	39.0
11	37.386973004605245	39.0	37.0	39.0	35.0	39.0
12	37.322092461135284	39.0	37.0	39.0	34.0	39.0
13	37.286207159758796	39.0	37.0	39.0	34.0	39.0
14	38.442348930107116	40.0	38.0	41.0	34.0	41.0
15	38.578977686181716	40.0	38.0	41.0	34.0	41.0
16	38.5326219372567	40.0	38.0	41.0	34.0	41.0
17	38.52135867491031	40.0	38.0	41.0	34.0	41.0
18	38.3002946339974	40.0	38.0	41.0	34.0	41.0
19	38.031137572195505	40.0	37.0	41.0	34.0	41.0
20	37.96895300613185	40.0	35.0	41.0	34.0	41.0
21	37.8408426837646	40.0	35.0	41.0	34.0	41.0
22	37.912509477648015	40.0	35.0	41.0	34.0	41.0
23	37.919159351703435	40.0	35.0	41.0	34.0	41.0
24	37.87085464213928	40.0	35.0	41.0	34.0	41.0
25	37.61132025545124	39.0	35.0	41.0	33.0	41.0
26	37.64086710938096	39.0	35.0	41.0	33.0	41.0
27	37.62533038190469	40.0	35.0	41.0	33.0	41.0
28	37.527794824822536	39.0	35.0	41.0	33.0	41.0
29	37.443508129150445	39.0	35.0	41.0	33.0	41.0
30	37.180407602473096	39.0	35.0	41.0	33.0	41.0
31	36.99396789049182	39.0	35.0	41.0	32.0	41.0
32	36.7141073200519	39.0	35.0	41.0	31.0	41.0
33	36.37631834719996	39.0	35.0	41.0	30.0	41.0
34	36.02458743607358	39.0	35.0	41.0	29.0	41.0
35	35.82403480650332	39.0	35.0	41.0	26.0	41.0
36	35.63939648372898	39.0	35.0	41.0	25.0	41.0
37	35.53069638449991	39.0	35.0	41.0	24.0	41.0
38	35.356537668880236	39.0	35.0	41.0	23.0	41.0
39	35.25598147724092	39.0	35.0	41.0	23.0	41.0
40	35.15563799201079	39.0	35.0	41.0	23.0	41.0
41	35.01686588810014	38.0	35.0	41.0	23.0	41.0
42	34.98086761824797	38.0	35.0	41.0	23.0	41.0
43	34.90576800753123	38.0	35.0	41.0	22.0	41.0
44	34.83096455741292	38.0	35.0	41.0	22.0	41.0
45	34.79098592982724	38.0	34.0	40.0	23.0	41.0
46	34.67661806986744	38.0	34.0	40.0	22.0	41.0
47	34.59855176449635	38.0	34.0	40.0	22.0	41.0
48	34.533960257486704	38.0	34.0	40.0	22.0	41.0
49	34.485760374526116	38.0	34.0	40.0	21.0	41.0
50	34.37520087525125	37.0	34.0	40.0	20.0	41.0
51	33.39231101951505	36.0	33.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	16.0
9	33.0
10	41.0
11	28.0
12	18.0
13	29.0
14	41.0
15	47.0
16	100.0
17	183.0
18	378.0
19	745.0
20	1250.0
21	1898.0
22	3107.0
23	4782.0
24	8212.0
25	13389.0
26	18807.0
27	20472.0
28	19510.0
29	18018.0
30	17929.0
31	20562.0
32	25571.0
33	35794.0
34	58007.0
35	70276.0
36	77070.0
37	113802.0
38	181003.0
39	271273.0
40	181.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.03757982851182	17.884130982367758	20.255654784622042	20.822634404498384
2	42.05012340024935	20.688904154899117	21.184744167111923	16.076228277739613
3	30.091036307660996	21.587665063735592	33.2182276162125	15.103071012390911
4	25.230440424395084	23.29867949011526	33.32152761875683	18.149352466732818
5	21.118896776327507	34.23697936544284	28.639136961555096	16.004986896674552
6	23.065618400630996	30.656591099916035	31.796453196956975	14.481337302495993
7	71.60125181283871	5.0294379563900975	18.26873266671755	5.100577564053634
8	72.56596188586113	4.728697554894028	17.984784876472535	4.720555682772307
9	67.02205938477978	5.716815510266391	20.21860926646821	7.042515838485612
10	34.97147800422359	23.362287866066204	26.654962725491693	15.011271404218506
11	28.08935704653589	23.10765081545938	31.35557082156578	17.44742131643895
12	23.614685901839554	21.10302012569015	34.89616568709767	20.386128285372617
13	23.151922245121238	20.655827799404626	37.34157697885658	18.85067297661756
14	19.53174057959952	23.020532783756963	37.818894231992466	19.628832404651046
15	19.311197618502405	22.57201740325166	38.93209169783477	19.184693280411164
16	23.436582449176907	23.423046586774547	35.10429229320917	18.036078670839377
17	22.469328041116455	23.609393684960438	34.26323690303539	19.65804137088772
18	23.81416176882172	22.610589522428313	34.57608833931252	18.999160369437448
19	21.536065949164186	25.65056102587589	32.766964353866115	20.04640867109381
20	22.910515736712213	25.478462203903007	32.93173549092945	18.679286568455332
21	21.993232068798818	26.48367809073099	34.01826832557311	17.50482151489708
22	22.11220517517747	22.22334172963896	34.68498587894054	20.979467216243037
23	20.306541485382795	25.614940335343356	34.52744065338524	19.55107752588861
24	20.52810218049513	24.009668473144544	34.88151031727858	20.58071902908175
25	22.094598376714245	25.122865938986845	32.611556369742765	20.17097931455614
26	21.30799175635448	23.729689845558863	33.30178357886167	21.660534819224996
27	21.920667633513983	22.52357326412742	33.54288476706613	22.01287433529247
28	20.339414294074242	24.170266900745492	33.12591914103249	22.364399664147776
29	22.50474518484594	23.85446403582424	32.33432562399817	21.306465155331654
30	23.7776251176755	21.868457878533444	33.90937078594509	20.444546217845964
31	23.253899193445793	22.334783604305013	32.70070986947562	21.71060733277358
32	24.721878736992085	22.965778693738393	31.5936188077246	20.718723761544922
33	23.326870722336718	22.610080655420706	31.965397043482685	22.09765157875989
34	21.827443197720275	24.684833218838257	33.13792840241203	20.349795181029435
35	22.004427142966186	26.932295244637817	31.681551026639188	19.381726585756812
36	23.181334758160954	27.735796249650154	29.263007912881967	19.81986107930692
37	23.024705493219347	27.171768058417932	29.250591557896342	20.552934890466375
38	22.542808437014987	27.021245197567616	29.81136300027988	20.62458336513752
39	23.567463043533575	25.0767625880976	29.02333155229881	22.33244281607002
40	24.572882477164594	24.437625626542502	31.227031015444112	19.76246088084879
41	21.88779482482253	25.69727501717426	30.17164084166603	22.243289316337176
42	23.05442332646363	25.209475103681655	30.625244892247412	21.110856677607305
43	22.786250413454443	24.30094394829911	30.93198992443325	21.980815713813197
44	23.732335953998422	23.7776251176755	29.91049029336183	22.579548634964254
45	22.331933949062414	23.338879983716257	30.12787827901178	24.20130778820955
46	22.38495789125512	24.107370938605197	29.73747551077526	23.770195659364425
47	21.71121797318271	24.384092817342186	31.54700658982775	22.357682619647353
48	21.34381599369005	24.55039055542834	31.279138997023125	22.826654453858485
49	22.694247258479	23.536015062463424	31.0552375136758	22.714500165381775
50	20.334936264407297	23.943821082360124	32.782026817291296	22.939215835941276
51	20.984148792713025	22.801923517288756	31.257257715696003	24.95666997430222
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	329.0
1	704.0
2	1079.0
3	16814.5
4	32550.0
5	21016.0
6	9482.0
7	9199.0
8	8916.0
9	8943.0
10	8970.0
11	8791.0
12	8612.0
13	8393.5
14	8175.0
15	7645.0
16	7115.0
17	6735.5
18	6356.0
19	6064.0
20	5772.0
21	5598.0
22	5424.0
23	5536.0
24	5648.0
25	5959.5
26	6855.5
27	7440.0
28	8412.0
29	9384.0
30	11066.0
31	12748.0
32	14441.5
33	16135.0
34	18410.5
35	20686.0
36	22184.5
37	23683.0
38	26908.0
39	30133.0
40	32059.5
41	33986.0
42	37340.5
43	40695.0
44	46286.5
45	51878.0
46	67460.0
47	83042.0
48	86638.5
49	90235.0
50	90055.0
51	89875.0
52	82080.0
53	74285.0
54	67772.5
55	61260.0
56	55425.5
57	49591.0
58	45498.0
59	41405.0
60	37587.0
61	33769.0
62	31046.5
63	28324.0
64	25261.0
65	22198.0
66	19112.0
67	16026.0
68	13400.0
69	10774.0
70	8910.0
71	7046.0
72	6162.5
73	5279.0
74	4431.5
75	3003.0
76	2422.0
77	1778.0
78	1134.0
79	799.0
80	464.0
81	342.5
82	221.0
83	149.5
84	78.0
85	69.5
86	61.0
87	39.5
88	18.0
89	12.0
90	6.0
91	7.0
92	8.0
93	5.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	982575.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.92288330875904
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.6736789384196	28.286691249383257
2	9.550391928210757	7.434575811493232
3	4.222733590502383	4.930829003613041
4	2.4820733290338293	3.8643780219906736
5	1.7058748019510999	3.319878292784755
6	1.340853385881201	3.1313927923685063
7	1.0816221696155557	2.9469897444479134
8	0.9136029851231442	2.8448049904385657
9	0.8093321181370761	2.835138563305218
>10	5.12836732867465	31.54360167315577
>50	0.054934964931452314	1.4364908205648375
>100	0.030489189246258885	2.2064940748090907
>500	0.002365540541587139	0.7122409002386395
>1k	0.0034168918934036454	2.571105760528223
>5k	0.0	0.0
>10k+	2.628378379541266E-4	1.9353883008782793
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18918	1.9253492099839706	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTT	3877	0.3945754776989034	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTC	3096	0.31509045111060224	No Hit
CGCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTG	2810	0.2859832582754497	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTC	2656	0.2703101544411368	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCT	2232	0.22715823219601558	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2161	0.2199323206879882	No Hit
CGTCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCT	1600	0.16283744243441978	No Hit
GCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGC	1286	0.13088059435666488	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCG	1130	0.11500394371930896	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTC	1094	0.1113401012645345	No Hit
CCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGC	1082	0.11011882044627637	No Hit
CTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGCT	1071	0.10899931302953973	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGC	1037	0.10553901737780831	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCC	990	0.10075566750629722	No Hit
GAACTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCT	983	0.10004325369564664	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.0709360608604943E-4	0.0	0.0	0.1153092639238735	0.0
2	4.0709360608604943E-4	0.0	0.0	0.40933262091952266	0.0
3	4.0709360608604943E-4	0.0	0.0	0.8508256367198432	0.0
4	4.0709360608604943E-4	0.0	0.0	1.3343510673485484	0.0
5	5.088670076075618E-4	0.0	0.0	2.5996997684655114	0.0
6	5.088670076075618E-4	0.0	0.0	4.296669465435208	0.0
7	5.088670076075618E-4	0.0	0.0	5.4977991501920975	0.0
8	5.088670076075618E-4	0.0	0.0	7.0830216522911735	0.0
9	5.088670076075618E-4	0.0	0.0	8.080808080808081	0.0
10	5.088670076075618E-4	0.0	0.0	9.488232450449075	0.0
11	6.106404091290741E-4	0.0	0.0	10.59420400478335	0.0
12	6.106404091290741E-4	0.0	0.0	11.531333485993436	0.0
13	6.106404091290741E-4	0.0	0.0	12.094445716611963	0.0
14	6.106404091290741E-4	0.0	0.0	12.48525557845457	0.0
15	6.106404091290741E-4	0.0	0.0	12.824873419331858	0.0
16	6.106404091290741E-4	0.0	0.0	13.205098847416227	0.0
17	6.106404091290741E-4	0.0	0.0	13.610258758873368	0.0
18	6.106404091290741E-4	0.0	0.0	14.049309213037173	0.0
19	6.106404091290741E-4	0.0	0.0	14.371014935246674	0.0
20	7.124138106505864E-4	0.0	0.0	14.709411495305702	0.0
21	7.124138106505864E-4	0.0	0.0	15.080680864056179	0.0
22	8.141872121720989E-4	0.0	0.0	15.486146095717885	0.0
23	8.141872121720989E-4	0.0	0.0	15.889982952955245	0.0
24	8.141872121720989E-4	0.0	0.0	16.227056458794493	0.0
25	8.141872121720989E-4	0.0	0.0	16.579090654657406	0.0
26	8.141872121720989E-4	0.0	0.0	16.89092435691932	0.0
27	8.141872121720989E-4	0.0	0.0	17.255171360964813	0.0
28	8.141872121720989E-4	0.0	0.0	17.587868610538635	0.0
29	8.141872121720989E-4	0.0	0.0	17.949876599750656	0.0
30	8.141872121720989E-4	0.0	0.0	18.46362873063125	0.0
31	9.159606136936112E-4	0.0	0.0	18.84782332137496	0.0
32	0.0010177340152151236	0.0	0.0	19.293896140243746	0.0
33	0.0010177340152151236	0.0	0.0	19.690914179579167	0.0
34	0.001119507416736636	0.0	0.0	20.107981579014325	0.0
35	0.001119507416736636	0.0	0.0	20.51415922448668	0.0
36	0.001119507416736636	0.0	0.0	20.890822583517796	0.0
37	0.001119507416736636	0.0	0.0	21.276645548685853	0.0
38	0.001119507416736636	0.0	0.0	21.72414319517594	0.0
39	0.001119507416736636	0.0	0.0	22.402055822710736	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	9505	0.0	43.982113	1
CGTTATT	1230	0.0	43.353657	1
CGGTCTA	65	0.0	41.53846	31
CCGACGG	40	3.457535E-7	39.375	2
TAGACGG	70	0.0	38.571426	2
TACGGTT	35	6.2473664E-6	38.571426	33
GTTATTT	1420	0.0	38.34507	2
CACGACC	470	0.0	38.29787	27
GTTTTTT	11140	0.0	37.930878	2
GACACGA	475	0.0	37.894737	25
AGACACG	475	0.0	37.894737	24
TCAAGCG	475	0.0	37.894737	17
GCGAGAC	480	0.0	37.500004	21
AAATGCG	30	1.13980124E-4	37.500004	1
GCGTAAG	90	0.0	37.5	1
AACACGT	430	0.0	37.151165	41
CGTTTTC	310	0.0	37.01613	1
CAACACG	55	2.748493E-9	36.81818	22
ACACGAC	490	0.0	36.734695	26
GTCAAGC	485	0.0	36.649487	16
>>END_MODULE