##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935806.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	564445
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05897651675539	33.0	31.0	34.0	30.0	34.0
2	32.19307284146374	33.0	31.0	34.0	30.0	34.0
3	32.086474324336294	33.0	31.0	34.0	30.0	34.0
4	35.75724472712133	37.0	35.0	37.0	33.0	37.0
5	32.15307248713338	37.0	35.0	37.0	0.0	37.0
6	33.906587887216645	37.0	35.0	37.0	17.0	37.0
7	35.53488648141094	37.0	35.0	37.0	32.0	37.0
8	35.777896872148744	37.0	35.0	37.0	35.0	37.0
9	37.731222705489465	39.0	38.0	39.0	35.0	39.0
10	37.36417720061299	39.0	37.0	39.0	34.0	39.0
11	37.328944361275234	39.0	37.0	39.0	34.0	39.0
12	37.286359166969326	39.0	37.0	39.0	34.0	39.0
13	37.231983629937375	39.0	37.0	39.0	34.0	39.0
14	38.382574032899576	40.0	38.0	41.0	34.0	41.0
15	38.53578648052512	40.0	38.0	41.0	34.0	41.0
16	38.49533789828947	40.0	38.0	41.0	34.0	41.0
17	38.50697765061255	40.0	38.0	41.0	34.0	41.0
18	38.37102286316647	40.0	38.0	41.0	34.0	41.0
19	38.18753820124193	40.0	37.0	41.0	34.0	41.0
20	38.19120906377061	40.0	37.0	41.0	34.0	41.0
21	38.104922534525066	40.0	36.0	41.0	34.0	41.0
22	38.18475316461303	40.0	36.0	41.0	34.0	41.0
23	38.19564705152849	40.0	36.0	41.0	34.0	41.0
24	38.16204058854273	40.0	36.0	41.0	34.0	41.0
25	37.88534223883638	40.0	36.0	41.0	34.0	41.0
26	37.93007821842695	40.0	36.0	41.0	34.0	41.0
27	37.91920736298488	40.0	36.0	41.0	34.0	41.0
28	37.84000566928576	40.0	36.0	41.0	34.0	41.0
29	37.78310730009124	40.0	36.0	41.0	33.0	41.0
30	37.593802762005154	40.0	35.0	41.0	33.0	41.0
31	37.53462073364101	40.0	35.0	41.0	33.0	41.0
32	37.3732976640771	40.0	35.0	41.0	33.0	41.0
33	37.20906908556192	40.0	35.0	41.0	33.0	41.0
34	36.99943307142414	40.0	35.0	41.0	32.0	41.0
35	36.90444418853919	40.0	35.0	41.0	31.0	41.0
36	36.76617562384289	40.0	35.0	41.0	31.0	41.0
37	36.686720583936435	40.0	35.0	41.0	31.0	41.0
38	36.56701007184048	40.0	35.0	41.0	31.0	41.0
39	36.45225841313148	39.0	35.0	41.0	30.0	41.0
40	36.39378327383536	39.0	35.0	41.0	30.0	41.0
41	36.22960607322237	39.0	35.0	41.0	30.0	41.0
42	36.21636297602069	39.0	35.0	41.0	30.0	41.0
43	36.16281125707553	39.0	35.0	41.0	30.0	41.0
44	36.11503512299693	39.0	35.0	41.0	30.0	41.0
45	36.07913791423434	39.0	35.0	41.0	30.0	41.0
46	35.988115759728586	39.0	35.0	41.0	29.0	41.0
47	35.90741170530344	39.0	35.0	41.0	29.0	41.0
48	35.854386166942746	39.0	35.0	41.0	29.0	41.0
49	35.82963973460656	39.0	35.0	41.0	29.0	41.0
50	35.71644181452577	38.0	35.0	41.0	28.0	41.0
51	34.72064771589792	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	9.0
9	29.0
10	19.0
11	15.0
12	12.0
13	8.0
14	23.0
15	23.0
16	39.0
17	91.0
18	194.0
19	304.0
20	531.0
21	886.0
22	1348.0
23	2151.0
24	3155.0
25	4876.0
26	6659.0
27	7428.0
28	7700.0
29	7654.0
30	8404.0
31	10444.0
32	13458.0
33	19875.0
34	32656.0
35	39873.0
36	43577.0
37	65949.0
38	106885.0
39	180012.0
40	155.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.50901327853024	19.592520086102276	22.04306885524719	15.855397780120295
2	36.95169591368512	23.00773326010506	23.4478115671146	16.592759259095217
3	31.06857178290179	23.597516144177025	29.254577505337103	16.079334567584088
4	27.147552020125964	24.872928274676894	29.731506169777393	18.248013535419748
5	22.22147419146241	35.74555536854787	24.973558096891637	17.05941234309809
6	24.471649142077617	33.124750861465685	27.609067313910124	14.79453268254657
7	77.17102640647006	5.14115635712957	13.016680101692813	4.6711371347075445
8	78.14756087838497	5.251175933881955	12.088157393545874	4.513105794187211
9	72.30412174791167	6.283163107122926	14.328588259263524	7.084126885701885
10	37.32285696569196	26.920426259422975	20.98060927105387	14.776107503831195
11	28.27786586824226	23.997732285696568	29.10930205777357	18.61509978828761
12	24.86034954690005	21.79964389798829	32.7776842739328	20.562322281178858
13	24.0274960359291	22.33539140217382	35.05159935866205	18.585513203235035
14	20.42696808369283	25.029187963397675	34.08799794488392	20.45584600802558
15	19.336870731426444	24.20625570250423	35.98614568292748	20.470727883141848
16	23.241768462826315	24.25373597073231	32.41839328898298	20.086102277458387
17	23.244957436065516	23.745449069439893	31.620618483643227	21.388975010851365
18	23.819858445021218	23.315292012507864	32.28870837725553	20.57614116521539
19	22.740391003552162	25.428518279017442	30.354418942501045	21.476671774929358
20	23.516374491757393	26.485131412272235	29.96022641709998	20.038267678870394
21	23.197122837477522	26.0020019665335	31.762173462427697	19.038701733561286
22	22.330962272674928	22.621336002622044	32.12642507241627	22.92127665228676
23	20.973699829035603	25.733951049260778	31.795657681439288	21.496691440264332
24	21.235727130189833	24.855034591501386	31.888846566095904	22.02039171221288
25	22.591217922029607	26.531371524240626	29.510935520732755	21.366475032997016
26	21.160431928708732	25.119719370354947	30.56595416736795	23.153894533568373
27	22.87397354923863	24.059208603141137	30.690855619236597	22.375962228383635
28	20.698030809024797	25.722789642923583	30.58136753802408	22.99781201002755
29	21.887694992426187	25.298479036930082	30.081053069829654	22.732772900814073
30	23.24194562800627	24.319109922135905	30.75693823136001	21.682006218497815
31	23.742968756920515	24.483164878774726	29.263258599154923	22.510607765149835
32	24.150448670818236	24.64367653181444	29.377884470586153	21.827990326781173
33	23.54100045177121	23.288540070334577	29.098672146976234	24.07178733091798
34	21.169113022526552	24.627023004898618	31.235284217239943	22.968579755334886
35	22.589269105050093	25.081097361124645	30.264419031083627	22.065214502741632
36	23.675823153717367	25.387061626907848	28.570719910708746	22.366395308666036
37	22.40980077775514	24.868853475537918	30.576584078165276	22.144761668541666
38	22.482615666716864	24.79869606427553	29.74709670561348	22.97159156339413
39	22.068580641160786	23.762634091895578	30.084950703788678	24.08383456315496
40	24.14672820203917	23.10730009124007	30.66410367706331	22.08186802965745
41	21.94403351965205	23.95290949516782	30.536544747495327	23.566512237684805
42	22.249820620255296	24.93883372162035	30.415363764405747	22.39598189371861
43	22.709564262239898	23.54649257234983	30.88538298682777	22.8585601785825
44	22.943599464961157	23.79470098946753	29.704399897244194	23.557299648327117
45	21.8800768896881	22.985410447430663	29.74337623683441	25.391136426046824
46	23.28889440069449	23.79948444932633	29.00566042749958	23.905960722479605
47	21.055195811815146	24.60044822790529	31.392961227400367	22.951394732879198
48	21.87493909946939	24.917573900025687	29.95721460904074	23.250272391464183
49	22.791591740559312	23.473323353028196	30.434320438660983	23.30076446775151
50	20.914172328570544	23.852456838133033	31.242370824438165	23.99100000885826
51	21.444959207717314	23.069032412369673	29.917175278370788	25.56883310154222
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	262.0
1	475.0
2	688.0
3	5327.0
4	9966.0
5	6420.5
6	2875.0
7	2868.5
8	2862.0
9	2766.5
10	2671.0
11	2709.0
12	2747.0
13	2665.0
14	2583.0
15	2426.0
16	2269.0
17	2196.5
18	2124.0
19	2127.0
20	2130.0
21	2160.0
22	2190.0
23	2250.5
24	2311.0
25	2896.5
26	4093.0
27	4704.0
28	5465.5
29	6227.0
30	7296.5
31	8366.0
32	9176.0
33	9986.0
34	12077.0
35	14168.0
36	15395.5
37	16623.0
38	18085.5
39	19548.0
40	21521.0
41	23494.0
42	25674.0
43	27854.0
44	31207.0
45	34560.0
46	41994.5
47	49429.0
48	50709.5
49	51990.0
50	51953.5
51	51917.0
52	46697.5
53	41478.0
54	37626.5
55	33775.0
56	31307.0
57	28839.0
58	26846.0
59	24853.0
60	22462.0
61	20071.0
62	17961.0
63	15851.0
64	14091.5
65	12332.0
66	10733.5
67	9135.0
68	7932.0
69	6729.0
70	5787.0
71	4845.0
72	4314.0
73	3783.0
74	2970.0
75	1742.0
76	1327.0
77	978.0
78	629.0
79	469.0
80	309.0
81	269.5
82	230.0
83	137.0
84	44.0
85	34.0
86	24.0
87	14.0
88	4.0
89	2.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	564445.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.806078343324124
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.69277627575067	27.821154928071895
2	9.649561578284997	7.48923285171316
3	4.036903392968659	4.699691679959183
4	2.4459181422416396	3.7966596419674743
5	1.8031327979619298	3.4986256310563943
6	1.4180658870428375	3.301774554514786
7	1.2310722027728207	3.344115904296347
8	1.0977140718371576	3.4078382616225653
9	0.9305203284663751	3.2498860289869667
>10	5.630303175762244	33.55040336426407
>50	0.03731369712243928	0.9937558386074477
>100	0.020269168804873457	1.4283340798277013
>500	0.0027639775643009262	0.7770923961215291
>1k	0.0032246404916844136	1.5675921834804898
>5k	4.6066292738348763E-4	1.073842655510013
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6007	1.0642312359928778	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTC	1698	0.30082647556449255	No Hit
CGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTG	1324	0.23456669826112378	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTT	1300	0.230314733942191	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGC	1207	0.21383837220632657	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCG	1195	0.21171239004686018	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTC	1041	0.18442895233370835	No Hit
GCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC	1004	0.17787384067535367	No Hit
CCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC	888	0.157322679800512	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCT	775	0.13730301446553694	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	683	0.12100381790962804	No Hit
CGTCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCT	678	0.12011799200985038	No Hit
CTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGCT	662	0.11728334913056189	Illumina Single End Adapter 1 (95% over 21bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	661	0.11710618395060636	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.5433035991106306E-4	0.0	0.0	0.12118098308958357	0.0
2	3.5433035991106306E-4	0.0	0.0	0.5382278167049048	0.0
3	3.5433035991106306E-4	0.0	0.0	0.9244479090079636	0.0
4	3.5433035991106306E-4	0.0	0.0	1.352833314140439	0.0
5	3.5433035991106306E-4	0.0	0.0	2.5593281896376086	0.0
6	3.5433035991106306E-4	0.0	0.0	3.7725553419730886	0.0
7	3.5433035991106306E-4	0.0	0.0	4.731904791432292	0.0
8	3.5433035991106306E-4	0.0	0.0	6.059049154479179	0.0
9	3.5433035991106306E-4	0.0	0.0	6.777099628838948	0.0
10	3.5433035991106306E-4	0.0	0.0	8.050562942359308	0.0
11	3.5433035991106306E-4	0.0	0.0	9.16227444658027	0.0
12	3.5433035991106306E-4	0.0	0.0	10.238552914810123	0.0
13	3.5433035991106306E-4	0.0	0.0	10.694753253195618	0.0
14	3.5433035991106306E-4	0.0	0.0	10.979634862564112	0.0
15	3.5433035991106306E-4	0.0	0.0	11.288079440866692	0.0
16	3.5433035991106306E-4	0.0	0.0	11.76908290444596	0.0
17	3.5433035991106306E-4	0.0	0.0	12.286936725455979	0.0
18	3.5433035991106306E-4	0.0	0.0	12.866798359450433	0.0
19	0.0012401562596887207	0.0	0.0	13.279770393926778	0.0
20	0.0012401562596887207	0.0	0.0	13.648273968234284	0.0
21	0.0012401562596887207	0.0	0.0	14.063726315230005	0.0
22	0.0012401562596887207	0.0	0.0	14.538528997510829	0.0
23	0.0012401562596887207	0.0	0.0	14.994552170716368	0.0
24	0.0012401562596887207	0.0	0.0	15.388036035397603	0.0
25	0.0012401562596887207	0.0	0.0	15.720752243354092	0.0
26	0.0014173214396442522	0.0	0.0	16.057543250449555	0.0
27	0.0014173214396442522	0.0	0.0	16.448546802611414	0.0
28	0.0014173214396442522	0.0	0.0	16.815278725119366	0.0
29	0.0014173214396442522	0.0	0.0	17.210179911240246	0.0
30	0.0015944866195997838	0.0	0.0	17.71297469195404	0.0
31	0.0015944866195997838	0.0	0.0	18.098663288717237	0.0
32	0.0015944866195997838	0.0	0.0	18.472658983603363	0.0
33	0.0015944866195997838	0.0	0.0	18.873760951022685	0.0
34	0.0015944866195997838	0.0	0.0	19.279646378300807	0.0
35	0.0015944866195997838	0.0	0.0	19.71299240847204	0.0
36	0.0015944866195997838	0.0	0.0	20.106299107973317	0.0
37	0.0015944866195997838	0.0	0.0	20.500668798554333	0.0
38	0.0015944866195997838	0.0	0.0	20.952617172620894	0.0
39	0.0015944866195997838	0.0	0.0	21.534959119134726	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTGCG	30	2.1635296E-6	45.000004	1
CACGAGG	45	3.8380676E-10	45.000004	2
CCGATTC	20	7.0302334E-4	45.0	11
ACTACGG	40	6.8048394E-9	45.0	2
ATTATCG	20	7.0302334E-4	45.0	30
TATCGCG	20	7.0302334E-4	45.0	32
ATCGCCT	20	7.0302334E-4	45.0	41
TACGTCG	20	7.0302334E-4	45.0	1
CGTTTTT	2910	0.0	42.835052	1
TACGAAT	90	0.0	42.500004	12
CGAATAT	90	0.0	42.500004	14
GCTACGA	90	0.0	42.500004	10
CGTTATT	375	0.0	41.4	1
TCACGAC	50	1.0786607E-9	40.5	25
CTACGAA	95	0.0	40.263157	11
TAATACG	80	0.0	39.375	4
CGTTCTG	195	0.0	38.076923	1
AGTACGG	95	0.0	37.894737	2
TTACGGG	155	0.0	37.74194	3
GTATACG	30	1.1391856E-4	37.500004	1
>>END_MODULE