##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935805.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	441186
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.998732960701382	33.0	31.0	34.0	30.0	34.0
2	32.111485858572124	33.0	31.0	34.0	30.0	34.0
3	32.076731809259584	33.0	31.0	34.0	30.0	34.0
4	35.70988879973526	37.0	35.0	37.0	33.0	37.0
5	31.94775219521925	37.0	35.0	37.0	0.0	37.0
6	33.76849446718617	37.0	35.0	37.0	17.0	37.0
7	35.44621996164883	37.0	35.0	37.0	32.0	37.0
8	35.70144338215628	37.0	35.0	37.0	33.0	37.0
9	37.63701250719651	39.0	37.0	39.0	35.0	39.0
10	37.258836409133565	39.0	37.0	39.0	34.0	39.0
11	37.23483066099106	39.0	37.0	39.0	34.0	39.0
12	37.137787690452555	39.0	37.0	39.0	34.0	39.0
13	37.044656902077584	39.0	37.0	39.0	33.0	39.0
14	38.1646221774943	40.0	38.0	41.0	33.0	41.0
15	38.31989909017965	40.0	38.0	41.0	33.0	41.0
16	38.302187739411494	40.0	37.0	41.0	34.0	41.0
17	38.302006409994874	40.0	37.0	41.0	34.0	41.0
18	38.23904883654513	40.0	37.0	41.0	34.0	41.0
19	38.11792758609747	40.0	37.0	41.0	34.0	41.0
20	38.18139288191375	40.0	37.0	41.0	34.0	41.0
21	38.11986554423758	40.0	37.0	41.0	34.0	41.0
22	38.20117592126677	40.0	37.0	41.0	34.0	41.0
23	38.2052059675511	40.0	37.0	41.0	34.0	41.0
24	38.164377382781865	40.0	37.0	41.0	34.0	41.0
25	37.898065668448226	40.0	36.0	41.0	33.0	41.0
26	37.935088602086196	40.0	36.0	41.0	34.0	41.0
27	37.91467997624585	40.0	36.0	41.0	34.0	41.0
28	37.85471207155259	40.0	36.0	41.0	33.0	41.0
29	37.83205269432847	40.0	36.0	41.0	33.0	41.0
30	37.669030748935825	40.0	36.0	41.0	33.0	41.0
31	37.66032693693816	40.0	36.0	41.0	33.0	41.0
32	37.57021981658529	40.0	36.0	41.0	33.0	41.0
33	37.52884951018391	40.0	36.0	41.0	33.0	41.0
34	37.444472399396176	40.0	36.0	41.0	33.0	41.0
35	37.3883146790696	40.0	35.0	41.0	33.0	41.0
36	37.31275017792949	40.0	35.0	41.0	33.0	41.0
37	37.23544264777214	40.0	35.0	41.0	33.0	41.0
38	37.13904566328034	40.0	35.0	41.0	32.0	41.0
39	37.043199466891515	39.0	35.0	41.0	32.0	41.0
40	36.95669400207622	39.0	35.0	41.0	32.0	41.0
41	36.822904625260094	39.0	35.0	41.0	31.0	41.0
42	36.81062862375506	39.0	35.0	41.0	31.0	41.0
43	36.75472476461175	39.0	35.0	41.0	31.0	41.0
44	36.69770572955624	39.0	35.0	41.0	31.0	41.0
45	36.66789970669967	39.0	35.0	41.0	31.0	41.0
46	36.523577357395745	39.0	35.0	41.0	31.0	41.0
47	36.437688412596955	39.0	35.0	41.0	31.0	41.0
48	36.395082799544866	38.0	35.0	41.0	31.0	41.0
49	36.36260670102859	38.0	35.0	41.0	31.0	41.0
50	36.25086924789091	38.0	35.0	40.0	31.0	41.0
51	35.19960288857761	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	8.0
9	22.0
10	22.0
11	10.0
12	11.0
13	13.0
14	10.0
15	19.0
16	38.0
17	67.0
18	93.0
19	195.0
20	394.0
21	579.0
22	876.0
23	1334.0
24	1839.0
25	2397.0
26	2988.0
27	3670.0
28	4178.0
29	5063.0
30	6282.0
31	8206.0
32	11023.0
33	16653.0
34	28176.0
35	35026.0
36	36168.0
37	55379.0
38	85994.0
39	134370.0
40	79.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.67166682532991	19.469792785809158	23.000503189131113	11.85803719972982
2	32.78209190681481	24.93302144673675	24.411019388647873	17.873867257800566
3	32.772118788900826	24.583735657976455	25.897693943144162	16.746451609978557
4	28.493877865571438	25.334212781003927	26.405189647903605	19.766719705521027
5	23.516838702950682	36.59748949422693	21.827981848925397	18.057689953896997
6	25.45230356357636	34.90840597843087	24.311061547737236	15.32822891025554
7	78.85585671349499	6.209625872081163	9.969491325654033	4.965026088769816
8	79.82778238656711	6.008123557864484	8.786770205763556	5.377323849804845
9	74.11386580716524	6.65932282529364	11.056334516507777	8.170476851033351
10	42.204875041365774	22.776561359607967	18.201393516566707	16.81717008245955
11	30.716296527994995	23.775459783402013	24.048587217182778	21.459656471420217
12	26.54594660755328	20.236136232790706	30.72717629299207	22.49074086666395
13	24.747158794703367	22.421155703036813	32.675334212781	20.156351289478813
14	21.91660660129741	24.98651362463904	31.70454184856274	21.392337925500808
15	19.815451986237097	22.8239336696994	35.161587176383655	22.199027167679844
16	23.547211380234188	22.963783982265983	30.331198179452656	23.157806458047173
17	23.81648556391182	23.111340795038828	28.34609439102782	24.72607925002153
18	24.383139990842864	22.652577370995452	29.55352164393249	23.41076099422919
19	24.109332571749782	24.564922731002344	26.92515175005553	24.40059294719234
20	25.515542197621865	25.363678811204345	27.646162842882593	21.474616148291197
21	23.92369658148717	25.778923175259415	29.12671752956803	21.170662713685385
22	23.249151151668457	22.47578118979297	28.69651348864198	25.578554169896595
23	22.854986332295223	25.002379948593113	27.925636806244984	24.21699691286668
24	23.291310241032125	23.745767091430825	28.37533375945746	24.587588908079585
25	22.966957247056797	26.000371725304067	26.598078814830934	24.434592212808205
26	22.08138970864896	24.08077318863246	27.088121563240904	26.74971553947768
27	22.592738663511536	24.030907599062527	28.470985026723422	24.905368710702515
28	20.217323305816596	24.909901946117962	28.548050028786044	26.324724719279395
29	21.80599565716047	24.384953285009043	27.56297797300912	26.24607308482137
30	22.747548652949096	23.54947799794191	28.756125534355125	24.946847814753866
31	23.573957469185334	24.24510297244246	26.13931539078756	26.041624167584647
32	23.86884443296025	26.261486085233894	24.99467344838685	24.874996033419013
33	23.141033487010013	23.19316569428767	26.019411314048952	27.646389504653367
34	21.3012198936503	23.821018799327266	28.245456564804865	26.632304742217567
35	21.12170377119854	25.808162543689058	27.526258766143986	25.543874918968417
36	22.408235982102788	24.446378624888368	26.110756007670233	27.03462938533861
37	20.283961866423684	25.628193097695757	28.08452670755645	26.00331832832411
38	20.908415044901695	26.765128539890203	25.40175798869411	26.924698426513988
39	21.574573989201834	24.192970765164805	26.84151355664051	27.390941688992854
40	22.811467272306917	23.30853653561083	28.62624833970253	25.25374785237972
41	20.30957464652097	23.857964667963174	28.068887045373152	27.76357364014271
42	21.06775826975471	24.43617884520361	28.61400860408082	25.882054280960865
43	22.201067123616795	22.762281668049305	28.190377754507168	26.84627345382673
44	21.944259337331648	22.827106934490217	27.69081521172476	27.537818516453378
45	21.804182362994293	22.524286808738264	27.45282035241372	28.21871047585372
46	23.4098543471461	23.16483297294112	27.680388770269225	25.744923909643553
47	20.264242292366486	24.350954019393182	29.83004900427484	25.55475468396549
48	20.662033700072076	24.71474616148291	28.056193986209898	26.567026152235112
49	22.208093638510743	22.70946947545933	29.4408707438586	25.641566142171328
50	21.771769729773837	22.459688204068126	29.20514250225528	26.563399563902752
51	20.71461923089128	23.088674617961587	27.317276613491813	28.87942953765532
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	170.0
1	389.0
2	608.0
3	1586.5
4	2565.0
5	1686.5
6	808.0
7	775.5
8	743.0
9	781.5
10	820.0
11	791.0
12	762.0
13	777.5
14	793.0
15	780.5
16	768.0
17	765.5
18	763.0
19	726.0
20	689.0
21	989.0
22	1289.0
23	1380.5
24	1472.0
25	1575.5
26	2199.5
27	2720.0
28	2950.5
29	3181.0
30	3949.0
31	4717.0
32	5754.5
33	6792.0
34	8001.0
35	9210.0
36	9848.0
37	10486.0
38	11913.0
39	13340.0
40	14663.5
41	15987.0
42	18302.0
43	20617.0
44	23262.0
45	25907.0
46	32672.5
47	39438.0
48	41414.5
49	43391.0
50	43177.0
51	42963.0
52	38124.0
53	33285.0
54	30351.0
55	27417.0
56	25739.0
57	24061.0
58	23275.0
59	22489.0
60	21464.0
61	20439.0
62	18471.0
63	16503.0
64	14602.5
65	12702.0
66	11439.5
67	10177.0
68	8734.0
69	7291.0
70	6148.0
71	5005.0
72	4251.0
73	3497.0
74	3038.5
75	2021.5
76	1463.0
77	1106.5
78	750.0
79	659.5
80	569.0
81	365.0
82	161.0
83	113.0
84	65.0
85	47.0
86	29.0
87	21.5
88	14.0
89	10.5
90	7.0
91	4.5
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	441186.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.32760818921243
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.8779388690887	26.830315395535052
2	10.378603997442992	7.748169271350918
3	4.053417500179102	4.539131408219474
4	2.1952441482937024	3.277728537886747
5	1.448198934261789	2.7028901199079534
6	1.1038891612535204	2.4723325257353848
7	0.8842578455294449	2.3105061277312173
8	0.800564309164823	2.390652069021763
9	0.7169854374353543	2.408701633736219
>10	6.4650475002185095	40.32263536513956
>50	0.04404326930247512	1.143514130371243
>100	0.02752704331404695	1.8538720496174368
>500	0.0012234241472909755	0.3564348157966275
>1k	0.003058560368227439	1.6431165499503728
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTC	1673	0.3792051425022553	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1480	0.3354594207431786	No Hit
GCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC	1480	0.3354594207431786	TruSeq Adapter, Index 23 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGC	1345	0.3048600816889022	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCG	1218	0.2760740368008051	No Hit
CCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC	908	0.2058088878613555	TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGCT	653	0.14801013631438895	TruSeq Adapter, Index 20 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.799853123172539E-4	0.0	0.0	0.15413000412524422	0.0
2	6.799853123172539E-4	0.0	0.0	0.8733278027861265	0.0
3	6.799853123172539E-4	0.0	0.0	1.2078805764462155	0.0
4	6.799853123172539E-4	0.0	0.0	1.6856835892344726	0.0
5	6.799853123172539E-4	0.0	0.0	3.168051570086086	0.0
6	6.799853123172539E-4	0.0	0.0	4.060192299846324	0.0
7	6.799853123172539E-4	0.0	0.0	4.9208270434691945	0.0
8	6.799853123172539E-4	0.0	0.0	6.25155829967406	0.0
9	6.799853123172539E-4	0.0	0.0	6.791013314112416	0.0
10	6.799853123172539E-4	0.0	0.0	8.171383498116441	0.0
11	6.799853123172539E-4	0.0	0.0	9.545180490768066	0.0
12	6.799853123172539E-4	0.0	0.0	10.968389749448079	0.0
13	6.799853123172539E-4	0.0	0.0	11.46160576264886	0.0
14	6.799853123172539E-4	0.0	0.0	11.699827283730672	0.0
15	6.799853123172539E-4	0.0	0.0	12.089232205917686	0.0
16	6.799853123172539E-4	0.0	0.0	12.776017371358112	0.0
17	6.799853123172539E-4	0.0	0.0	13.557547156981409	0.0
18	6.799853123172539E-4	0.0	0.0	14.402315576650212	0.0
19	6.799853123172539E-4	0.0	0.0	14.979396445036787	0.0
20	6.799853123172539E-4	0.0	0.0	15.519984768329005	0.0
21	6.799853123172539E-4	0.0	0.0	16.126531666915994	0.0
22	6.799853123172539E-4	0.0	0.0	16.791103978820725	0.0
23	6.799853123172539E-4	0.0	0.0	17.417370451464915	0.0
24	6.799853123172539E-4	0.0	0.0	17.96543861319262	0.0
25	6.799853123172539E-4	0.0	0.0	18.46137456764267	0.0
26	6.799853123172539E-4	0.0	0.0	18.895431858671852	0.0
27	9.06647083089672E-4	0.0	0.0	19.39590104853735	0.0
28	9.06647083089672E-4	0.0	0.0	19.853757825497635	0.0
29	9.06647083089672E-4	0.0	0.0	20.328840897036624	0.0
30	9.06647083089672E-4	0.0	0.0	20.90297516240316	0.0
31	9.06647083089672E-4	0.0	0.0	21.39143127841772	0.0
32	0.00113330885386209	0.0	0.0	21.8846472916185	0.0
33	0.00113330885386209	0.0	0.0	22.3456773333696	0.0
34	0.00113330885386209	0.0	0.0	22.827106934490214	0.0
35	0.00113330885386209	0.0	0.0	23.360215419346943	0.0
36	0.00113330885386209	0.0	0.0	23.808325740164012	0.0
37	0.00113330885386209	0.0	0.0	24.303581709301746	0.0
38	0.00113330885386209	0.0	0.0	24.819010576038224	0.0
39	0.00113330885386209	0.0	0.0	25.478822990756733	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	90	0.0	45.000004	4
TTGCTAG	20	7.0285547E-4	45.000004	1
TACGGGT	30	2.1624492E-6	45.000004	4
GCCGATA	20	7.0285547E-4	45.000004	9
CTAACGG	20	7.0285547E-4	45.000004	2
GTATACC	20	7.0285547E-4	45.000004	13
CGGCTAC	20	7.0285547E-4	45.000004	25
CCGGTAT	40	6.7993824E-9	45.000004	42
GCGTACG	40	6.7993824E-9	45.000004	1
TTTCGCG	25	3.886885E-5	45.0	1
TAGCCGT	50	2.1827873E-11	45.0	44
ATAACGC	25	3.886885E-5	45.0	11
TGCGTAG	25	3.886885E-5	45.0	1
TTACGGG	125	0.0	44.999996	3
TATAGCG	60	3.6379788E-12	41.250004	1
TAACGGG	105	0.0	38.57143	3
GTTGACG	35	6.2395393E-6	38.571426	1
ATTACGG	35	6.2395393E-6	38.571426	2
CGTTAGG	35	6.2395393E-6	38.571426	2
AACGAGC	70	0.0	38.571426	15
>>END_MODULE