FastQCFastQC Report
Sat 14 Jan 2017
SRR2935802.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935802.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences719928
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99251.3786100832305452No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTC25860.35920258692535917No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTT19650.27294396106277297No Hit
CGCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTG15660.2175217521752175No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTC14420.20029780755853366No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGC13770.19126912691269127No Hit
GCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC13460.1869631407585203No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCG12600.17501750175017503No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCT11770.16348857107933015No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11430.15876587658765876No Hit
CCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC10500.14584791812514583No Hit
CGTCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCT10180.14140302919180808No Hit
CTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGCT9240.12834616795012835Illumina Single End Adapter 1 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCT7900.10973319554177641No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCG253.889347E-545.00000421
TCGACGG351.2110831E-745.0000042
GTACGAG253.889347E-545.0000041
CGTTATT6100.043.524591
ACACGAC1300.043.2692326
CGTTTTT47400.043.148731
CGAATAT700.041.78571714
CATACGG650.041.538463
TCACGAC1200.041.25000425
CGACGGT1200.041.25000428
CACGACC1400.040.17857427
TATGCGG900.040.02
CGTTTCT2100.039.6428571
GACGGTC1250.039.629
CGGTCTA1250.039.631
TAACGTT403.4558252E-739.37521
TAACGGG1950.039.230773
TAAGGGC3500.039.2142874
GCGAGAC1450.038.79310221
ACGGTCT1350.038.33333230
Produced by FastQC (version 0.11.5)