##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935802.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	719928
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00096398528742	33.0	31.0	34.0	30.0	34.0
2	32.13388977786668	33.0	31.0	34.0	30.0	34.0
3	32.01208176373193	33.0	31.0	34.0	30.0	34.0
4	35.69902962518474	37.0	35.0	37.0	33.0	37.0
5	32.04421414363659	37.0	35.0	37.0	0.0	37.0
6	33.828981509262036	37.0	35.0	37.0	17.0	37.0
7	35.489268371281575	37.0	35.0	37.0	32.0	37.0
8	35.747740051782955	37.0	35.0	37.0	35.0	37.0
9	37.6922178328944	39.0	37.0	39.0	35.0	39.0
10	37.29903129201809	39.0	37.0	39.0	34.0	39.0
11	37.3095823471236	39.0	37.0	39.0	34.0	39.0
12	37.27720827638319	39.0	37.0	39.0	34.0	39.0
13	37.25121401028992	39.0	37.0	39.0	34.0	39.0
14	38.40876309853208	40.0	38.0	41.0	34.0	41.0
15	38.556279239035014	40.0	38.0	41.0	34.0	41.0
16	38.49944577791113	40.0	38.0	41.0	34.0	41.0
17	38.51159004789368	40.0	38.0	41.0	34.0	41.0
18	38.35327838339389	40.0	38.0	41.0	34.0	41.0
19	38.139659799313264	40.0	37.0	41.0	34.0	41.0
20	38.10544804480448	40.0	36.0	41.0	34.0	41.0
21	38.013720816526096	40.0	36.0	41.0	34.0	41.0
22	38.09782783833939	40.0	36.0	41.0	34.0	41.0
23	38.1095456767899	40.0	36.0	41.0	34.0	41.0
24	38.05992404796035	40.0	36.0	41.0	34.0	41.0
25	37.79760198242047	40.0	35.0	41.0	33.0	41.0
26	37.83492515918258	40.0	35.0	41.0	34.0	41.0
27	37.827314675912035	40.0	35.0	41.0	34.0	41.0
28	37.739075296418534	40.0	35.0	41.0	33.0	41.0
29	37.6807152937516	40.0	35.0	41.0	33.0	41.0
30	37.46339633963397	40.0	35.0	41.0	33.0	41.0
31	37.368453512017865	40.0	35.0	41.0	33.0	41.0
32	37.20674845262304	40.0	35.0	41.0	33.0	41.0
33	36.98946561322799	40.0	35.0	41.0	32.0	41.0
34	36.74905823915725	40.0	35.0	41.0	31.0	41.0
35	36.63298690980209	40.0	35.0	41.0	31.0	41.0
36	36.46869270260359	40.0	35.0	41.0	30.0	41.0
37	36.4040751297352	40.0	35.0	41.0	30.0	41.0
38	36.26005795023947	39.0	35.0	41.0	30.0	41.0
39	36.16403306997366	39.0	35.0	41.0	29.0	41.0
40	36.09385521885522	39.0	35.0	41.0	29.0	41.0
41	35.922093598248715	39.0	35.0	41.0	28.0	41.0
42	35.896261848407065	39.0	35.0	41.0	28.0	41.0
43	35.82769388049916	39.0	35.0	41.0	28.0	41.0
44	35.77166049938327	39.0	35.0	41.0	27.0	41.0
45	35.74612044537787	39.0	35.0	41.0	27.0	41.0
46	35.643305997266395	39.0	35.0	41.0	27.0	41.0
47	35.55728350612839	39.0	35.0	41.0	27.0	41.0
48	35.49978192263671	38.0	35.0	41.0	26.0	41.0
49	35.47014701470147	38.0	35.0	40.0	27.0	41.0
50	35.37703770377038	38.0	35.0	40.0	26.0	41.0
51	34.40037198164261	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	13.0
9	32.0
10	32.0
11	29.0
12	24.0
13	23.0
14	20.0
15	29.0
16	55.0
17	106.0
18	217.0
19	391.0
20	739.0
21	1200.0
22	1919.0
23	3017.0
24	4672.0
25	7153.0
26	9941.0
27	11390.0
28	11399.0
29	10911.0
30	11523.0
31	13846.0
32	17580.0
33	25061.0
34	40482.0
35	51049.0
36	56188.0
37	83512.0
38	135911.0
39	221311.0
40	153.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.75324199086575	18.50962874065184	22.880482492693712	17.85664677578869
2	38.31772066095498	22.636847018035137	23.06230623062306	15.983126090386818
3	30.203714815926038	23.29413496905246	30.736129168472402	15.7660210465491
4	26.191785845251193	24.19297485304086	31.779705748352615	17.835533553355333
5	21.996644108855328	34.580124679134585	26.634330099676635	16.788901112333456
6	23.31066439977331	32.64368103477015	29.445166738896113	14.600487826560435
7	74.81109222033314	5.110511051105111	15.175406429531844	4.902990299029903
8	76.4968163483015	4.554761031658722	14.138080474714137	4.810342145325644
9	70.73193430454157	5.795718460734962	16.08160816081608	7.39073907390739
10	35.7969130246358	22.95507328510629	24.056711226678225	17.191302463579692
11	27.693185985265195	24.154776588769987	29.321682168216824	18.830355257747996
12	24.457862452911957	20.908340834083408	33.58974786367526	21.04404884932938
13	23.355252191885857	20.995988487737662	35.80927537198164	19.83948394839484
14	20.166738896111834	24.488004355991155	34.90154571012657	20.44371103777044
15	19.47291951417364	23.762376237623762	36.479897989798985	20.284806258403616
16	22.40154571012657	23.313025747019147	33.90186240846307	20.383566134391216
17	22.310842195330643	23.69625851474036	32.05070507050705	21.942194219421943
18	22.88978897889789	23.32386016379416	32.81314242535365	20.97320843195431
19	22.182218221822183	25.75007500750075	30.7683546132391	21.299352157437966
20	22.455856696780792	26.084969608071916	30.819331933193318	20.639841761953974
21	22.118045137847115	25.993154871042663	32.86495316198286	19.023846829127358
22	21.19434165638786	22.807141825293638	33.23207320732073	22.766443310997765
23	20.172295007278507	25.072785056283408	32.63020746519096	22.124712471247125
24	20.74040737407074	24.84387327621651	32.811475592003646	21.604243757709103
25	22.41182451578491	25.592975964263093	30.17523974619684	21.819959773755155
26	20.88958895889589	24.911518929670745	31.165199853318665	23.033692258114698
27	21.871353802046873	23.516935026836016	31.449672745052283	23.16203842606483
28	20.345367870120345	24.277844451111775	32.179745752353014	23.19704192641486
29	22.191246902468027	23.27191052438577	31.53593137091487	23.000911202231332
30	22.924514673689593	22.852701936860353	31.854296540765187	22.368486848684867
31	22.591842517585093	22.79102910291029	31.663721927748327	22.953406451756287
32	23.244407774110744	23.119673078418952	31.67775110844418	21.958168039026123
33	22.333761153893168	22.116517207276285	31.066856685668565	24.482864953161982
34	21.2669878098921	22.655737796001823	33.1049771643831	22.972297229722972
35	22.01525708126368	22.873120645397872	32.02973075085286	23.08189152248558
36	22.95618450733962	23.815437099265484	31.13339111688947	22.094987276505428
37	21.49562178440066	23.778766765565447	32.68826882688269	22.037342623151204
38	21.307547421408806	24.24478558967008	31.813598026469315	22.6340689624518
39	21.669250258359167	22.774360769410272	31.47370292584814	24.082686046382413
40	23.067028925114734	22.511278905668345	32.69313042415353	21.728561745063395
41	21.877187718771875	23.41414697025258	31.759287039815092	22.94937827116045
42	22.15582669378049	24.24339656187841	31.715810469935885	21.88496627440522
43	21.845656787901014	22.88714982609372	32.21586047493638	23.051332911068883
44	22.424464668689094	23.175928703981512	30.986015268193484	23.413591359135914
45	21.609383160538275	22.860758298052026	30.859474836372524	24.67038370503717
46	23.103421453256438	22.888677756664556	30.20663177428854	23.801269015790467
47	21.171144892267005	23.961979531286463	32.24127968352391	22.625595892922625
48	20.914174750808414	23.911418919669742	31.473286217510637	23.7011201120112
49	21.351162894067187	22.671989421164337	32.38712760164905	23.589720083119424
50	20.701931304241537	22.341539709526508	33.1338689424498	23.822660043782157
51	20.752630818637417	21.977892233667813	32.00208909779867	25.2673878498961
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	217.0
1	479.0
2	741.0
3	8764.5
4	16788.0
5	10824.5
6	4861.0
7	4748.0
8	4635.0
9	4563.5
10	4492.0
11	4466.5
12	4441.0
13	4245.0
14	4049.0
15	3845.0
16	3641.0
17	3482.5
18	3324.0
19	3127.0
20	2930.0
21	3001.5
22	3073.0
23	3077.0
24	3081.0
25	3535.5
26	4480.5
27	4971.0
28	5915.0
29	6859.0
30	8170.0
31	9481.0
32	10820.5
33	12160.0
34	14200.0
35	16240.0
36	17994.5
37	19749.0
38	21547.0
39	23345.0
40	25625.0
41	27905.0
42	30216.0
43	32527.0
44	37835.5
45	43144.0
46	54970.0
47	66796.0
48	68436.5
49	70077.0
50	69253.5
51	68430.0
52	61703.0
53	54976.0
54	48895.5
55	42815.0
56	39725.5
57	36636.0
58	33923.0
59	31210.0
60	28428.5
61	25647.0
62	22484.0
63	19321.0
64	17343.0
65	15365.0
66	12954.5
67	10544.0
68	8908.5
69	7273.0
70	6379.5
71	5486.0
72	4561.0
73	3636.0
74	2958.5
75	1826.5
76	1372.0
77	1031.5
78	691.0
79	529.5
80	368.0
81	307.0
82	246.0
83	159.5
84	73.0
85	50.5
86	28.0
87	19.0
88	10.0
89	6.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	719928.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.911072314619865
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.87086736169096	27.965725172252093
2	9.640818672827274	7.5026918510103915
3	4.249071177621984	4.960077475872479
4	2.592394924419906	4.034914654886259
5	1.8319336820651166	3.5641251989211793
6	1.4697467731805005	3.43136537852434
7	1.2003347202534074	3.269441777107054
8	1.0281020251687085	3.2003641798517397
9	0.8613504748534968	3.0164463553730916
>10	5.183082049261591	31.867549189342103
>50	0.04012546923315154	1.04777566495434
>100	0.02602732807327983	2.010311220959182
>500	0.001807453338422142	0.4924502927916287
>1k	0.003976397344528712	2.2407121291547116
>5k	3.614906676844284E-4	1.3960494589994046
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9925	1.3786100832305452	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTC	2586	0.35920258692535917	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTT	1965	0.27294396106277297	No Hit
CGCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTG	1566	0.2175217521752175	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTC	1442	0.20029780755853366	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGC	1377	0.19126912691269127	No Hit
GCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	1346	0.1869631407585203	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCG	1260	0.17501750175017503	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCT	1177	0.16348857107933015	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1143	0.15876587658765876	No Hit
CCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	1050	0.14584791812514583	No Hit
CGTCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCT	1018	0.14140302919180808	No Hit
CTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGCT	924	0.12834616795012835	Illumina Single End Adapter 1 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCT	790	0.10973319554177641	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.1670833750041673E-4	0.0	0.0	0.13320776522096653	0.0
2	4.1670833750041673E-4	0.0	0.0	0.5524163527463858	0.0
3	4.1670833750041673E-4	0.0	0.0	0.957317954017624	0.0
4	5.556111166672222E-4	0.0	0.0	1.447366958918114	0.0
5	5.556111166672222E-4	0.0	0.0	2.796946361302797	0.0
6	5.556111166672222E-4	0.0	0.0	4.30654176528764	0.0
7	5.556111166672222E-4	0.0	0.0	5.405262748497072	0.0
8	5.556111166672222E-4	0.0	0.0	6.867353402006867	0.0
9	5.556111166672222E-4	0.0	0.0	7.708270827082708	0.0
10	6.945138958340278E-4	0.0	0.0	9.043126534875709	0.0
11	6.945138958340278E-4	0.0	0.0	10.262137324843595	0.0
12	6.945138958340278E-4	0.0	0.0	11.347106932915514	0.0
13	6.945138958340278E-4	0.0	0.0	11.85951928526186	0.0
14	6.945138958340278E-4	0.0	0.0	12.167327843895501	0.0
15	6.945138958340278E-4	0.0	0.0	12.48916558322499	0.0
16	6.945138958340278E-4	0.0	0.0	12.954212087875455	0.0
17	6.945138958340278E-4	0.0	0.0	13.4657910235468	0.0
18	6.945138958340278E-4	0.0	0.0	14.050849529397384	0.0
19	8.334166750008335E-4	0.0	0.0	14.432137658210266	0.0
20	8.334166750008335E-4	0.0	0.0	14.818981898189818	0.0
21	8.334166750008335E-4	0.0	0.0	15.242357569090242	0.0
22	8.334166750008335E-4	0.0	0.0	15.699208809769866	0.0
23	9.72319454167639E-4	0.0	0.0	16.14994832816615	0.0
24	9.72319454167639E-4	0.0	0.0	16.54832149881655	0.0
25	9.72319454167639E-4	0.0	0.0	16.89571734951273	0.0
26	9.72319454167639E-4	0.0	0.0	17.23741818626307	0.0
27	9.72319454167639E-4	0.0	0.0	17.633013301330134	0.0
28	9.72319454167639E-4	0.0	0.0	17.993882721605495	0.0
29	9.72319454167639E-4	0.0	0.0	18.38711648942672	0.0
30	9.72319454167639E-4	0.0	0.0	18.871053772043872	0.0
31	0.0011112222333344445	0.0	0.0	19.247758109144247	0.0
32	0.0011112222333344445	0.0	0.0	19.63932504361547	0.0
33	0.0011112222333344445	0.0	0.0	20.014918158482516	0.0
34	0.0011112222333344445	0.0	0.0	20.41426364858708	0.0
35	0.0011112222333344445	0.0	0.0	20.85208520852085	0.0
36	0.0011112222333344445	0.0	0.0	21.236290295696236	0.0
37	0.0011112222333344445	0.0	0.0	21.6343856607883	0.0
38	0.0011112222333344445	0.0	0.0	22.103043637697102	0.0
39	0.0011112222333344445	0.0	0.0	22.70754853263104	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATCG	25	3.889347E-5	45.000004	21
TCGACGG	35	1.2110831E-7	45.000004	2
GTACGAG	25	3.889347E-5	45.000004	1
CGTTATT	610	0.0	43.52459	1
ACACGAC	130	0.0	43.26923	26
CGTTTTT	4740	0.0	43.14873	1
CGAATAT	70	0.0	41.785717	14
CATACGG	65	0.0	41.53846	3
TCACGAC	120	0.0	41.250004	25
CGACGGT	120	0.0	41.250004	28
CACGACC	140	0.0	40.178574	27
TATGCGG	90	0.0	40.0	2
CGTTTCT	210	0.0	39.642857	1
GACGGTC	125	0.0	39.6	29
CGGTCTA	125	0.0	39.6	31
TAACGTT	40	3.4558252E-7	39.375	21
TAACGGG	195	0.0	39.23077	3
TAAGGGC	350	0.0	39.214287	4
GCGAGAC	145	0.0	38.793102	21
ACGGTCT	135	0.0	38.333332	30
>>END_MODULE