##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935800.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	445082
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.927474038491784	33.0	31.0	34.0	30.0	34.0
2	31.97385200929267	33.0	31.0	34.0	30.0	34.0
3	32.00226475121438	33.0	31.0	34.0	30.0	34.0
4	35.67775825578208	37.0	35.0	37.0	33.0	37.0
5	34.72290948634184	37.0	35.0	37.0	33.0	37.0
6	35.22840510287992	37.0	35.0	37.0	33.0	37.0
7	35.805505502356866	37.0	35.0	37.0	35.0	37.0
8	35.80222520793921	37.0	35.0	37.0	35.0	37.0
9	37.67208963741513	39.0	37.0	39.0	35.0	39.0
10	37.21395158644924	39.0	37.0	39.0	34.0	39.0
11	37.146622420138314	39.0	37.0	39.0	34.0	39.0
12	36.92294228928602	39.0	35.0	39.0	33.0	39.0
13	36.72476532414252	39.0	35.0	39.0	33.0	39.0
14	37.7242373315479	40.0	36.0	41.0	33.0	41.0
15	37.864575516421695	40.0	36.0	41.0	33.0	41.0
16	37.98303458688511	40.0	36.0	41.0	33.0	41.0
17	37.95777856664615	40.0	36.0	41.0	33.0	41.0
18	37.96271248893462	40.0	36.0	41.0	34.0	41.0
19	37.88482122395424	40.0	36.0	41.0	34.0	41.0
20	37.831878170764035	40.0	36.0	41.0	33.0	41.0
21	37.78503736390148	39.0	35.0	41.0	33.0	41.0
22	37.83501916500779	40.0	35.0	41.0	33.0	41.0
23	37.865777542115836	40.0	36.0	41.0	34.0	41.0
24	37.82649938662988	40.0	35.0	41.0	34.0	41.0
25	37.685417518569615	39.0	35.0	41.0	33.0	41.0
26	37.72396996508508	40.0	35.0	41.0	33.0	41.0
27	37.74180712767535	40.0	35.0	41.0	34.0	41.0
28	37.5984537680697	40.0	35.0	41.0	33.0	41.0
29	37.57026570384783	40.0	35.0	41.0	33.0	41.0
30	37.44390247190405	39.0	35.0	41.0	33.0	41.0
31	37.42821996845525	39.0	35.0	41.0	33.0	41.0
32	37.40803941745566	40.0	35.0	41.0	33.0	41.0
33	37.27389784354344	39.0	35.0	41.0	33.0	41.0
34	37.18077118373693	39.0	35.0	41.0	33.0	41.0
35	37.171453799524585	39.0	35.0	41.0	33.0	41.0
36	37.0799740272579	39.0	35.0	41.0	32.0	41.0
37	37.05740964586301	39.0	35.0	41.0	32.0	41.0
38	36.96524460661182	39.0	35.0	41.0	32.0	41.0
39	36.910162621719145	39.0	35.0	41.0	32.0	41.0
40	36.782911912861	39.0	35.0	41.0	31.0	41.0
41	36.71760933940263	39.0	35.0	41.0	31.0	41.0
42	36.7836106605075	39.0	35.0	41.0	31.0	41.0
43	36.65563424267888	39.0	35.0	41.0	31.0	41.0
44	36.52377988775102	39.0	35.0	41.0	31.0	41.0
45	36.51552073550492	39.0	35.0	41.0	31.0	41.0
46	36.367011022687954	39.0	35.0	40.0	31.0	41.0
47	36.23748882228443	38.0	35.0	40.0	31.0	41.0
48	36.23498591270822	38.0	35.0	40.0	31.0	41.0
49	36.23000930165677	38.0	35.0	40.0	31.0	41.0
50	36.08645148534428	38.0	35.0	40.0	30.0	41.0
51	34.85885072862978	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	7.0
10	13.0
11	10.0
12	10.0
13	9.0
14	9.0
15	16.0
16	35.0
17	54.0
18	111.0
19	218.0
20	405.0
21	637.0
22	961.0
23	1461.0
24	2005.0
25	2579.0
26	3188.0
27	3785.0
28	4430.0
29	5275.0
30	6719.0
31	8830.0
32	11833.0
33	17039.0
34	31944.0
35	43653.0
36	30869.0
37	46667.0
38	83713.0
39	138508.0
40	83.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.298762924584686	20.91681982196539	23.653618883711317	12.13079836973861
2	30.834318170584297	26.76585438188918	24.628046067915573	17.77178137961095
3	29.737441639967464	26.8516812632279	26.789670218072175	16.62120687873246
4	27.559640695422416	25.14458009984677	29.2471948989175	18.04858430581331
5	25.64695943668807	31.016307107454356	24.607600397230172	18.729133058627397
6	24.596815867637872	35.339780085467396	25.052012887512866	15.011391159381867
7	77.23475674145438	5.686367905239933	12.068113291483368	5.010762061822316
8	77.1837549035908	7.2692223006097745	9.186172435641073	6.360850360158353
9	72.41227459209763	6.420165272916003	11.082227544587289	10.085332590399073
10	41.1939822324875	23.663729380204096	20.45218633869714	14.690102048611267
11	32.483227809706975	25.17603497782431	23.119559991192634	19.22117722127608
12	27.915305494268473	20.92805370695737	29.94436980151972	21.21227099725444
13	23.573633622568426	26.364130654576012	31.606535424932936	18.455700297922633
14	18.89854004430644	31.26435128807725	29.327404837760234	20.509703829856072
15	16.403943542987584	26.023968617018888	38.176560723641934	19.395527116351595
16	19.202753649889235	25.61954875730764	31.85929783725246	23.31839975555066
17	19.299589738520094	25.52361137947614	28.148970302101635	27.02782857990213
18	21.566812407601297	24.94304420309067	31.538233404181703	21.951909985126335
19	22.246237771916185	27.144885661518554	27.315191357996955	23.29368520856831
20	23.738996409650355	26.355817579681943	29.810911247815007	20.094274762852688
21	21.343482773960755	28.92523175504739	29.395706858511467	20.335578612480397
22	19.66514035615909	25.44856902772972	28.77919125015166	26.10709936595953
23	19.171523449611534	27.726351548703388	28.768182042859518	24.333942958825563
24	21.73082712848419	25.597530342723363	29.19506967255472	23.476572856237727
25	18.65835958317793	28.357246529852926	27.818469405637614	25.16592448133153
26	19.32363025240293	27.464377350690434	28.411169177814426	24.800823219092212
27	20.240315267748414	27.824760381233123	26.95368493895507	24.981239412063395
28	17.2833320601597	26.157202493023757	32.99773974233962	23.561725704476927
29	20.794595153252658	23.28177729047681	32.194516965413115	23.729110590857417
30	20.086860398757985	27.530432594443273	29.230793426829216	23.151913579969534
31	21.87911441037831	28.025397567189863	26.938182177666135	23.157305844765684
32	22.595162239767056	28.073703272655376	26.29155975752783	23.039574730049743
33	20.554639369823988	29.224053095834023	25.139637190450298	25.081670343891687
34	20.21200587756863	27.724778804804508	28.651124961243095	23.412090356383768
35	18.980547404747888	26.00509569023236	27.14578437231791	27.86857253270184
36	22.487766299243734	28.07595004965377	27.188473135287428	22.247810515815065
37	17.743696667131	27.31923555659407	31.988712192360058	22.94835558391487
38	18.56781447014258	28.666627722532027	27.751515451085417	25.014042356239973
39	19.70311088743198	28.523058672334535	29.237084402424724	22.536746037808765
40	21.139250744806574	26.928071681173353	27.70365910101959	24.22901847300048
41	18.657685550078412	28.203117627762975	24.939449359893235	28.19974746226538
42	21.02466511788839	24.961467774477512	27.502572559663164	26.511294547970937
43	22.12109229310554	25.002583793548155	27.641198700464187	25.23512521288212
44	20.319851173491628	24.555250493167552	29.443113853177614	25.681784480163206
45	19.319586053805814	25.45463532562539	27.04939763908673	28.176380981482062
46	23.587563639958482	26.232469522470016	27.345972202874975	22.833994634696527
47	17.890860560525926	25.42228173684849	32.06038437860889	24.626473324016697
48	19.14164131553287	25.088860030286554	29.05666820945354	26.712830444727036
49	20.55284194822527	21.99325068189682	33.68952238014568	23.76438498973223
50	20.811670658440466	22.6675084591154	30.94485959890537	25.57596128353876
51	19.00593598482976	23.43298538246885	26.658233763666022	30.902844869035366
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	458.0
1	527.5
2	597.0
3	1047.5
4	1498.0
5	1050.0
6	602.0
7	598.5
8	595.0
9	654.5
10	714.0
11	711.0
12	708.0
13	737.0
14	766.0
15	789.5
16	813.0
17	846.0
18	879.0
19	878.0
20	877.0
21	863.0
22	849.0
23	1157.0
24	1465.0
25	1653.5
26	2139.5
27	2437.0
28	3194.0
29	3951.0
30	4523.5
31	5096.0
32	5785.0
33	6474.0
34	7968.0
35	9462.0
36	10292.0
37	11122.0
38	12898.0
39	14674.0
40	18097.5
41	21521.0
42	26946.0
43	32371.0
44	45511.5
45	58652.0
46	58185.0
47	57718.0
48	56449.0
49	55180.0
50	46500.0
51	37820.0
52	32436.5
53	27053.0
54	24324.0
55	21595.0
56	19432.0
57	17269.0
58	16168.5
59	15068.0
60	12976.5
61	10885.0
62	9513.0
63	8141.0
64	6923.0
65	5705.0
66	4831.5
67	3958.0
68	3183.0
69	2408.0
70	1921.0
71	1434.0
72	1197.0
73	960.0
74	906.0
75	636.5
76	421.0
77	267.5
78	114.0
79	81.0
80	48.0
81	30.5
82	13.0
83	9.0
84	5.0
85	4.5
86	4.0
87	5.0
88	6.0
89	3.0
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	445082.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.27618539649119
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.74776541003529	22.439964127482213
2	12.296392684177725	7.69168514596801
3	4.646452784106645	4.359699561352863
4	2.274404074197436	2.8453873396453564
5	1.369297598449264	2.141320277603466
6	0.848322654882568	1.5919377978090483
7	0.6513000358860774	1.4259126469780026
8	0.5020537963182816	1.256186209013024
9	0.384016064941909	1.0809501878118732
>10	4.652265337906561	35.786748143899956
>50	0.5630151715623638	10.787572460115802
>100	0.053209607529520975	2.7837010488515967
>500	0.006471438753590388	1.3610404546493668
>1k	0.005033341252792525	4.4478945988194285
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGC	4195	0.9425229508270386	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCG	3651	0.820298282114307	No Hit
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	3623	0.8140073065187988	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTC	3457	0.7767108083454285	No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	2368	0.5320367932201258	No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCT	1469	0.3300515410643432	No Hit
TCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	1015	0.2280478653371738	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTT	895	0.2010865413564242	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	759	0.1705303741782413	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCT	735	0.1651381093820914	No Hit
ACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	725	0.16289133238369558	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTTAACAT	663	0.14896131499364162	No Hit
GAACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT	618	0.1388508185008605	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTC	596	0.13390790910438974	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTTAACATCGTA	532	0.11952853631465664	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCC	529	0.11885450321513788	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.4935539967916023E-4	0.0	0.0	0.35004785635006586	0.0
2	4.4935539967916023E-4	0.0	0.0	2.1717346466493814	0.0
3	4.4935539967916023E-4	0.0	0.0	2.859697763558176	0.0
4	4.4935539967916023E-4	0.0	0.0	3.7759334235039836	0.0
5	4.4935539967916023E-4	0.0	0.0	6.447351274596591	0.0
6	4.4935539967916023E-4	0.0	0.0	7.628032587253585	0.0
7	4.4935539967916023E-4	0.0	0.0	8.803770990514108	0.0
8	4.4935539967916023E-4	0.0	0.0	10.4879550285116	0.0
9	4.4935539967916023E-4	0.0	0.0	11.010555358338463	0.0
10	4.4935539967916023E-4	0.0	0.0	13.137354465019929	0.0
11	4.4935539967916023E-4	0.0	0.0	15.085984155728607	0.0
12	4.4935539967916023E-4	0.0	0.0	17.562831118760137	0.0
13	4.4935539967916023E-4	0.0	0.0	18.312355925424978	0.0
14	4.4935539967916023E-4	0.0	0.0	18.661280393275845	0.0
15	4.4935539967916023E-4	0.0	0.0	19.229040940770464	0.0
16	4.4935539967916023E-4	0.0	0.0	20.197401827079055	0.0
17	4.4935539967916023E-4	0.0	0.0	21.37246619724006	0.0
18	4.4935539967916023E-4	0.0	0.0	22.79669813652316	0.0
19	4.4935539967916023E-4	0.0	0.0	23.71630396196656	0.0
20	4.4935539967916023E-4	0.0	0.0	24.60917314112905	0.0
21	4.4935539967916023E-4	0.0	0.0	25.52990235507165	0.0
22	4.4935539967916023E-4	0.0	0.0	26.523202466062433	0.0
23	4.4935539967916023E-4	0.0	0.0	27.458311052794766	0.0
24	4.4935539967916023E-4	0.0	0.0	28.16290031949169	0.0
25	4.4935539967916023E-4	0.0	0.0	28.753353314670107	0.0
26	4.4935539967916023E-4	0.0	0.0	29.386045717418362	0.0
27	4.4935539967916023E-4	0.0	0.0	30.0191874755663	0.0
28	4.4935539967916023E-4	0.0	0.0	30.648285035117123	0.0
29	4.4935539967916023E-4	0.0	0.0	31.25087062608688	0.0
30	4.4935539967916023E-4	0.0	0.0	31.9446753631915	0.0
31	6.740330995187404E-4	0.0	0.0	32.57691841054008	0.0
32	6.740330995187404E-4	0.0	0.0	33.15681155382604	0.0
33	0.0011233884991979006	0.0	0.0	33.72973968841696	0.0
34	0.0013480661990374807	0.0	0.0	34.31502509649907	0.0
35	0.0013480661990374807	0.0	0.0	34.9418758790515	0.0
36	0.0013480661990374807	0.0	0.0	35.51884821223954	0.0
37	0.0013480661990374807	0.0	0.0	36.10211152102309	0.0
38	0.0013480661990374807	0.0	0.0	36.67122013471675	0.0
39	0.0013480661990374807	0.0	0.0	37.29829559496902	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTAAG	45	3.8380676E-10	45.000004	1
CTTACGG	35	1.2096825E-7	45.000004	2
GCGACGT	20	7.0286234E-4	45.0	17
TACTTCG	20	7.0286234E-4	45.0	14
TAGCCGT	20	7.0286234E-4	45.0	44
ATCAAGT	25	3.8869406E-5	45.0	11
CGCCTCT	20	7.0286234E-4	45.0	39
AACGGCC	25	3.8869406E-5	45.0	17
CCGTATA	20	7.0286234E-4	45.0	17
CGCTCCG	25	3.8869406E-5	45.0	10
TTAAGCG	20	7.0286234E-4	45.0	1
GCTTACG	25	3.8869406E-5	45.0	1
CCTCACG	25	3.8869406E-5	45.0	24
ACGTGAC	20	7.0286234E-4	45.0	20
CGAATAT	20	7.0286234E-4	45.0	14
ACGTCGC	20	7.0286234E-4	45.0	20
TCGAGAG	25	3.8869406E-5	45.0	1
GCTACGA	20	7.0286234E-4	45.0	10
ATAGTCC	20	7.0286234E-4	45.0	37
TTCGAAC	20	7.0286234E-4	45.0	17
>>END_MODULE