SRR2935798.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935798.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1287134
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	3947	0.30665027883654694	TruSeq Adapter, Index 22 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	3375	0.2622104613816433	TruSeq Adapter, Index 22 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2686	0.20868068126550926	No Hit
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT	2491	0.19353074349679208	Illumina Paired End PCR Primer 2 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1466	0.11389645522533007	No Hit
TCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	1405	0.1091572439233211	TruSeq Adapter, Index 22 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACG	35	1.2120836E-7	45.000004	1
CGTACAG	20	7.0336025E-4	45.0	30
TCGTTTA	55	1.8189894E-12	44.999996	38
TACGTAG	90	0.0	42.5	1
ATAACGC	70	0.0	41.785717	11
CGTTTTT	1605	0.0	41.635513	1
CGCATCG	65	0.0	41.53846	21
CGTACAT	60	3.6379788E-12	41.249996	35
CGTTAGG	130	0.0	39.80769	2
AACACGT	125	0.0	39.6	41
TATAGCG	40	3.4586446E-7	39.375	1
CGAATAT	120	0.0	39.374996	14
CTATACG	35	6.2488725E-6	38.57143	1
TCGTGAC	35	6.2488725E-6	38.57143	17
TAATGCG	70	0.0	38.57143	1
TAGCCGT	135	0.0	38.333336	44
CGGTCTA	95	0.0	37.894737	31
GGTACGA	30	1.139998E-4	37.499996	8
ATCGGAT	30	1.139998E-4	37.499996	27
AATGCGG	175	0.0	37.285713	2