##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935798.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1287134
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06772566026537	33.0	31.0	34.0	30.0	34.0
2	32.10501004557412	33.0	31.0	34.0	30.0	34.0
3	32.12788800544465	33.0	31.0	34.0	30.0	34.0
4	35.77547015306875	37.0	35.0	37.0	35.0	37.0
5	34.84541702728698	37.0	35.0	37.0	33.0	37.0
6	35.33326833103624	37.0	35.0	37.0	33.0	37.0
7	35.92189857466278	37.0	35.0	37.0	35.0	37.0
8	35.934194885691774	37.0	35.0	37.0	35.0	37.0
9	37.797979075993645	39.0	38.0	39.0	35.0	39.0
10	37.34556308822547	39.0	37.0	39.0	34.0	39.0
11	37.27670467876693	39.0	37.0	39.0	34.0	39.0
12	37.186684525465104	39.0	37.0	39.0	34.0	39.0
13	37.14039408484276	39.0	37.0	39.0	34.0	39.0
14	38.11405727764164	40.0	37.0	41.0	33.0	41.0
15	38.2791302226497	40.0	38.0	41.0	33.0	41.0
16	38.31255720072657	40.0	37.0	41.0	34.0	41.0
17	38.32178234744789	40.0	37.0	41.0	34.0	41.0
18	38.3206457136553	40.0	37.0	41.0	34.0	41.0
19	38.240055037004694	40.0	37.0	41.0	34.0	41.0
20	38.24885287778895	40.0	37.0	41.0	34.0	41.0
21	38.15665812572739	40.0	37.0	41.0	34.0	41.0
22	38.22732986619886	40.0	37.0	41.0	34.0	41.0
23	38.23742205551248	40.0	37.0	41.0	34.0	41.0
24	38.195097013986114	40.0	37.0	41.0	34.0	41.0
25	38.031033287909416	40.0	36.0	41.0	34.0	41.0
26	38.07174622067322	40.0	37.0	41.0	34.0	41.0
27	38.08556529467794	40.0	37.0	41.0	34.0	41.0
28	37.98343529111965	40.0	36.0	41.0	34.0	41.0
29	37.93548457270183	40.0	36.0	41.0	34.0	41.0
30	37.79185073193622	40.0	36.0	41.0	33.0	41.0
31	37.71315962440585	40.0	36.0	41.0	33.0	41.0
32	37.65350460791184	40.0	36.0	41.0	33.0	41.0
33	37.54740454373826	40.0	36.0	41.0	33.0	41.0
34	37.412957003699695	40.0	35.0	41.0	33.0	41.0
35	37.36695324651512	40.0	35.0	41.0	33.0	41.0
36	37.325936538076064	40.0	35.0	41.0	33.0	41.0
37	37.24310289371581	40.0	35.0	41.0	32.0	41.0
38	37.152771195539856	39.0	35.0	41.0	32.0	41.0
39	37.07022501153726	39.0	35.0	41.0	32.0	41.0
40	36.954353625962796	39.0	35.0	41.0	31.0	41.0
41	36.86176031399994	39.0	35.0	41.0	31.0	41.0
42	36.92286739376009	39.0	35.0	41.0	32.0	41.0
43	36.841832318934934	39.0	35.0	41.0	31.0	41.0
44	36.711764276291355	39.0	35.0	41.0	31.0	41.0
45	36.6477763775955	39.0	35.0	41.0	31.0	41.0
46	36.52439062288775	39.0	35.0	41.0	31.0	41.0
47	36.40760247184831	39.0	35.0	41.0	31.0	41.0
48	36.35228422215558	39.0	35.0	41.0	31.0	41.0
49	36.32290810436209	39.0	35.0	40.0	31.0	41.0
50	36.19455705466564	38.0	35.0	40.0	30.0	41.0
51	34.98576760461615	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	14.0
9	31.0
10	29.0
11	33.0
12	27.0
13	42.0
14	36.0
15	52.0
16	98.0
17	172.0
18	258.0
19	491.0
20	821.0
21	1299.0
22	2078.0
23	3163.0
24	4476.0
25	6458.0
26	8726.0
27	10448.0
28	11974.0
29	14658.0
30	18178.0
31	23251.0
32	31053.0
33	45905.0
34	81415.0
35	95830.0
36	95819.0
37	147397.0
38	260470.0
39	422134.0
40	295.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.382145137957664	21.058957342436763	25.123025263880837	11.435872255724734
2	32.950026959120024	23.764891611906762	25.213458738561794	18.07162269041141
3	32.359101694151505	23.87296116799028	26.76691004976949	17.00102708808873
4	28.865292968719654	25.183314247001476	26.63265829354209	19.318734490736784
5	24.7157638598623	30.578945160332953	25.752252679208226	18.95303830059652
6	26.148792588805826	33.53077457358752	25.532617427556104	14.787815410050548
7	80.46131948965687	5.982438502906457	8.790226969375373	4.7660150380613056
8	81.74176115307343	4.949601207022734	8.239390770502528	5.069246869401321
9	76.3167626680672	6.7938536314012365	10.478473880730368	6.410909819801201
10	41.43344826568174	23.323756500877142	19.609535603907595	15.633259629533521
11	30.26576875445758	25.9847848009609	23.60088382406183	20.148562620519698
12	26.52552104132126	22.652653103717252	29.71749639120713	21.104329463754357
13	25.402094886779462	23.469895131353844	31.343589711716106	19.784420270150584
14	21.34610693214537	26.087182841879713	31.51761976608496	21.049090459889957
15	20.482249711374262	25.729022774629524	33.369486005342104	20.41924150865411
16	24.74303374784599	25.169329689061122	28.983773251269874	21.103863311823012
17	24.59720588532352	25.234280191495213	26.993304504426114	23.17520941875516
18	25.403571034562056	24.399557466433176	29.26315364212273	20.933717856882033
19	24.54818224054372	26.997499871808216	27.114892466518636	21.339425421129423
20	24.421699683172072	27.783820487998916	27.390543641920733	20.40393618690828
21	23.820985227645295	26.31349960454778	29.72153637461212	20.1439787931948
22	22.454616224884123	23.679818884436273	29.89548873699242	23.970076153687184
23	21.687019377935787	25.40574641024167	29.396084634544657	23.51114957727789
24	22.74083351073004	25.38430342139979	29.206982334395644	22.667880733474526
25	22.38508189512514	26.824402121302054	27.15249538898048	23.638020594592327
26	20.60562458920361	26.5336787001198	27.7712343858526	25.08946232482399
27	22.38104191172015	25.801820167907923	28.772140274439177	23.044997645932746
28	20.897901850157016	26.13294342314009	28.292858397027814	24.676296329675075
29	22.70820287553588	26.290269699969077	27.232051985263382	23.76947543923166
30	25.05302478219051	24.827407247419462	27.9199368519517	22.19963111843833
31	24.76657442037892	25.478932263462855	27.07495878440007	22.679534531758154
32	24.50397549905449	26.31971496363238	26.649983606990414	22.526325930322717
33	25.838646170484193	25.712085921123983	25.70625902198217	22.74300888640965
34	22.308866054350208	24.98652043998527	29.44580750722147	23.25880599844305
35	22.350353576239925	25.831110047594112	28.131492136793838	23.687044239372124
36	25.6864475649	26.30526425376068	26.15516333186754	21.853124849471772
37	23.439051411896507	26.8212167497712	28.432548592454243	21.30718324587805
38	23.57384701204381	27.599146631197684	26.543467890678045	22.283538466080454
39	23.002966280123125	25.990844776068382	27.017544404856057	23.988644538952432
40	24.072940346537347	23.21156926940008	30.229486595801212	22.486003788261364
41	21.40103516805554	24.67893785728603	28.814327024225918	25.105699950432513
42	22.17601275391684	25.05737553354973	29.092697419227527	23.673914293305902
43	22.956118011022937	25.174146592351686	28.496178331082856	23.373557065542517
44	23.65208284452124	24.30749245999251	27.422164281263644	24.618260414222608
45	23.305110423623336	23.463835156246358	27.419600445641244	25.81145397448906
46	23.82339367929058	23.98996530275791	28.460129248392164	23.726511769559348
47	21.49356632642755	25.181993483196003	30.297544777777606	23.026895412598844
48	21.63582035747638	25.666092263897934	29.106992745122106	23.59109463350358
49	23.771961582865497	23.995869893888283	29.39150080721976	22.84066771602646
50	21.94829753545474	23.7467116865843	29.57073622482197	24.73425455313899
51	21.558128368918855	23.71889795468071	27.546937614887028	27.176036061513408
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	548.0
1	977.5
2	1407.0
3	3180.0
4	4953.0
5	3448.5
6	1944.0
7	1962.0
8	1980.0
9	1999.5
10	2019.0
11	2085.5
12	2152.0
13	2213.5
14	2275.0
15	2112.0
16	1949.0
17	2011.5
18	2074.0
19	2196.0
20	2318.0
21	2646.0
22	2974.0
23	3454.5
24	3935.0
25	4957.0
26	7267.0
27	8555.0
28	10183.0
29	11811.0
30	14775.0
31	17739.0
32	19713.5
33	21688.0
34	26286.0
35	30884.0
36	34229.5
37	37575.0
38	41342.0
39	45109.0
40	49911.5
41	54714.0
42	61025.0
43	67336.0
44	76152.5
45	84969.0
46	99041.0
47	113113.0
48	125766.0
49	138419.0
50	137025.0
51	135631.0
52	119954.0
53	104277.0
54	94499.0
55	84721.0
56	78531.0
57	72341.0
58	65482.0
59	58623.0
60	53229.0
61	47835.0
62	42122.0
63	36409.0
64	31772.0
65	27135.0
66	22648.5
67	18162.0
68	14859.0
69	11556.0
70	10315.0
71	9074.0
72	7461.0
73	5848.0
74	4621.5
75	2510.5
76	1626.0
77	1388.5
78	1151.0
79	848.0
80	545.0
81	384.0
82	223.0
83	167.0
84	111.0
85	62.5
86	14.0
87	15.5
88	17.0
89	13.0
90	9.0
91	5.5
92	2.0
93	2.0
94	2.0
95	5.0
96	8.0
97	4.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1287134.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.254125915912027
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.33419670471999	20.868195725103153
2	11.834650366370065	6.924247039771686
3	4.535086740248663	3.9800999561645227
4	2.201053026912648	2.575595295876076
5	1.2995541737565524	1.90086607168116
6	0.8880084778133313	1.5586747094609141
7	0.6443383577367131	1.3194688814777242
8	0.5222654710028647	1.2222735880200724
9	0.4224364124202469	1.1122207200367242
>10	5.53646897585862	40.288088970842864
>50	0.7184060171149883	12.890976702946185
>100	0.05686140251949855	3.0079980048126096
>500	0.0034704142334503673	0.6360865002275953
>1k	0.0032034592924157232	1.7152078335787198
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	3947	0.30665027883654694	TruSeq Adapter, Index 22 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	3375	0.2622104613816433	TruSeq Adapter, Index 22 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2686	0.20868068126550926	No Hit
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT	2491	0.19353074349679208	Illumina Paired End PCR Primer 2 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1466	0.11389645522533007	No Hit
TCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	1405	0.1091572439233211	TruSeq Adapter, Index 22 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5538397711504785E-4	0.0	0.0	0.20401916195205783	0.0
2	1.5538397711504785E-4	0.0	0.0	1.00688817170551	0.0
3	1.5538397711504785E-4	0.0	0.0	1.4202095508315373	0.0
4	1.5538397711504785E-4	0.0	0.0	1.9361620468420537	0.0
5	1.5538397711504785E-4	0.0	0.0	3.2795342209902	0.0
6	1.5538397711504785E-4	0.0	0.0	4.114490022017909	0.0
7	1.5538397711504785E-4	0.0	0.0	4.896848346792176	0.0
8	1.5538397711504785E-4	0.0	0.0	6.071706597759052	0.0
9	1.5538397711504785E-4	0.0	0.0	6.487125660576132	0.0
10	1.5538397711504785E-4	0.0	0.0	7.594624957463636	0.0
11	2.330759656725718E-4	0.0	0.0	9.072248887839184	0.0
12	2.330759656725718E-4	0.0	0.0	10.260081700895167	0.0
13	3.107679542300957E-4	0.0	0.0	10.705334487318337	0.0
14	3.107679542300957E-4	0.0	0.0	10.891484491902164	0.0
15	3.107679542300957E-4	0.0	0.0	11.2511983989235	0.0
16	3.107679542300957E-4	0.0	0.0	11.964799313824358	0.0
17	3.107679542300957E-4	0.0	0.0	12.83417266578305	0.0
18	3.107679542300957E-4	0.0	0.0	13.849140804298543	0.0
19	3.107679542300957E-4	0.0	0.0	14.454672163115884	0.0
20	3.107679542300957E-4	0.0	0.0	15.057639686310827	0.0
21	3.107679542300957E-4	0.0	0.0	15.830131128538287	0.0
22	3.107679542300957E-4	0.0	0.0	16.648305460037573	0.0
23	3.107679542300957E-4	0.0	0.0	17.451096777802466	0.0
24	3.107679542300957E-4	0.0	0.0	18.06253272775018	0.0
25	3.107679542300957E-4	0.0	0.0	18.633102691716637	0.0
26	3.107679542300957E-4	0.0	0.0	19.154571318914737	0.0
27	3.107679542300957E-4	0.0	0.0	19.74083506456981	0.0
28	3.107679542300957E-4	0.0	0.0	20.304956593485993	0.0
29	3.107679542300957E-4	0.0	0.0	20.898756462031148	0.0
30	3.107679542300957E-4	0.0	0.0	21.607928933584226	0.0
31	3.107679542300957E-4	0.0	0.0	22.179975045333276	0.0
32	3.107679542300957E-4	0.0	0.0	22.77750412932919	0.0
33	3.107679542300957E-4	0.0	0.0	23.36594325066388	0.0
34	3.107679542300957E-4	0.0	0.0	23.94319472564628	0.0
35	3.8845994278761964E-4	0.0	0.0	24.56473063410647	0.0
36	3.8845994278761964E-4	0.0	0.0	25.116266060876335	0.0
37	3.8845994278761964E-4	0.0	0.0	25.721020499808102	0.0
38	3.8845994278761964E-4	0.0	0.0	26.331446453904565	0.0
39	3.8845994278761964E-4	0.0	0.0	27.151096933186444	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACG	35	1.2120836E-7	45.000004	1
CGTACAG	20	7.0336025E-4	45.0	30
TCGTTTA	55	1.8189894E-12	44.999996	38
TACGTAG	90	0.0	42.5	1
ATAACGC	70	0.0	41.785717	11
CGTTTTT	1605	0.0	41.635513	1
CGCATCG	65	0.0	41.53846	21
CGTACAT	60	3.6379788E-12	41.249996	35
CGTTAGG	130	0.0	39.80769	2
AACACGT	125	0.0	39.6	41
TATAGCG	40	3.4586446E-7	39.375	1
CGAATAT	120	0.0	39.374996	14
CTATACG	35	6.2488725E-6	38.57143	1
TCGTGAC	35	6.2488725E-6	38.57143	17
TAATGCG	70	0.0	38.57143	1
TAGCCGT	135	0.0	38.333336	44
CGGTCTA	95	0.0	37.894737	31
GGTACGA	30	1.139998E-4	37.499996	8
ATCGGAT	30	1.139998E-4	37.499996	27
AATGCGG	175	0.0	37.285713	2
>>END_MODULE