FastQCFastQC Report
Sat 14 Jan 2017
SRR2935797.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935797.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1826980
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213711.169744605852281No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTT87060.4765240998806774No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTC63380.3469112962375067No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTC62230.34061675552003856No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCT49610.27154101303790956No Hit
CGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG48360.264699120953705No Hit
CGTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCT41770.2286286658857787No Hit
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC33240.18193959430316697TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC28540.15621408006655793TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGC28500.15599513951986338No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCG27210.1489343068889643No Hit
CTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGCT22940.1255624035293216TruSeq Adapter, Index 16 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCC20720.11341120318777435No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTC20590.1126996464110171No Hit
GAACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCT20200.11056497608074528No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCGA207.034381E-445.018
CGTTTTT138000.043.8586921
CGACGGT900.042.50000428
TAACGCG850.042.352941
CGTTTCT10850.040.023041
CCGGTAT451.929402E-840.00000442
CGTTATT3300.039.5454521
TCCGATC403.4597178E-739.37514
GCTATCG403.4597178E-739.3751
CGAATAT2300.039.13043614
TTGTACG750.039.01
TTACGCG750.039.01
TATAGCG1450.038.7931021
CACGACC4250.038.6470627
CGTAGCG356.250313E-638.5714262
CGTTCTG7650.038.529411
AACGAGC9950.038.4422115
ACAACGA10100.038.3168313
CGGTCTA1000.038.25000431
CGTTTTC7200.038.1250041
Produced by FastQC (version 0.11.5)