##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935797.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1826980
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05395680303014	33.0	31.0	34.0	30.0	34.0
2	32.11320977788481	33.0	31.0	34.0	30.0	34.0
3	32.06868055479534	33.0	31.0	34.0	30.0	34.0
4	35.774782975183086	37.0	35.0	37.0	35.0	37.0
5	34.84535572365324	37.0	35.0	37.0	33.0	37.0
6	35.349871372428815	37.0	35.0	37.0	33.0	37.0
7	35.934924848657346	37.0	35.0	37.0	35.0	37.0
8	35.93940710899955	37.0	35.0	37.0	35.0	37.0
9	37.81395964925724	39.0	38.0	39.0	35.0	39.0
10	37.46433732170029	39.0	37.0	39.0	35.0	39.0
11	37.38073158983678	39.0	37.0	39.0	35.0	39.0
12	37.366345006513484	39.0	37.0	39.0	35.0	39.0
13	37.341635376413535	39.0	37.0	39.0	34.0	39.0
14	38.48764956376096	40.0	38.0	41.0	34.0	41.0
15	38.57835115874284	40.0	38.0	41.0	34.0	41.0
16	38.56744189865242	40.0	38.0	41.0	34.0	41.0
17	38.53647877918751	40.0	38.0	41.0	34.0	41.0
18	38.409053738957184	40.0	38.0	41.0	34.0	41.0
19	38.22641791371553	40.0	37.0	41.0	34.0	41.0
20	38.1390097319073	40.0	36.0	41.0	34.0	41.0
21	38.03197845625021	40.0	36.0	41.0	34.0	41.0
22	38.08389144927695	40.0	36.0	41.0	34.0	41.0
23	38.081402642612396	40.0	36.0	41.0	34.0	41.0
24	38.03305235963174	40.0	36.0	41.0	34.0	41.0
25	37.88576776976212	40.0	36.0	41.0	34.0	41.0
26	37.909968363091004	40.0	36.0	41.0	34.0	41.0
27	37.92114089918883	40.0	36.0	41.0	34.0	41.0
28	37.79941488138896	40.0	35.0	41.0	34.0	41.0
29	37.71947804573668	40.0	35.0	41.0	33.0	41.0
30	37.545638704309845	40.0	35.0	41.0	33.0	41.0
31	37.410474663105234	40.0	35.0	41.0	33.0	41.0
32	37.17556240352932	40.0	35.0	41.0	32.0	41.0
33	36.85824365893442	40.0	35.0	41.0	31.0	41.0
34	36.5924142574084	40.0	35.0	41.0	30.0	41.0
35	36.44068736384635	40.0	35.0	41.0	30.0	41.0
36	36.341189832401014	40.0	35.0	41.0	30.0	41.0
37	36.303099650789825	40.0	35.0	41.0	30.0	41.0
38	36.189653417114584	39.0	35.0	41.0	29.0	41.0
39	36.106264983743664	39.0	35.0	41.0	29.0	41.0
40	35.96914087729477	39.0	35.0	41.0	28.0	41.0
41	35.86027050104544	39.0	35.0	41.0	27.0	41.0
42	35.86421854645371	39.0	35.0	41.0	27.0	41.0
43	35.756888964301744	39.0	35.0	41.0	27.0	41.0
44	35.59573011198809	39.0	35.0	41.0	26.0	41.0
45	35.53661671173193	39.0	35.0	41.0	26.0	41.0
46	35.41470678387284	39.0	35.0	41.0	25.0	41.0
47	35.31718738026689	38.0	35.0	41.0	24.0	41.0
48	35.26674238360573	38.0	35.0	41.0	24.0	41.0
49	35.240377015621405	38.0	35.0	40.0	24.0	41.0
50	35.122593569716145	38.0	35.0	40.0	24.0	41.0
51	34.04857798114922	37.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	11.0
9	31.0
10	42.0
11	46.0
12	47.0
13	31.0
14	49.0
15	84.0
16	126.0
17	256.0
18	517.0
19	997.0
20	1844.0
21	3004.0
22	4558.0
23	7054.0
24	11894.0
25	19590.0
26	27921.0
27	30740.0
28	29188.0
29	27939.0
30	29287.0
31	34340.0
32	42537.0
33	57863.0
34	98699.0
35	124297.0
36	127972.0
37	193354.0
38	343977.0
39	608268.0
40	415.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.83125157363518	19.635628195163605	22.461931712443487	18.07118851875773
2	37.671622021040186	22.624002452134125	22.961170894043722	16.743204632781968
3	29.699011483431676	22.768120066995806	31.718628556415506	15.814239893157014
4	25.47729039179411	23.797687987826905	32.38771086711403	18.33731075326495
5	23.480224195119813	28.80743084215481	29.967323123405837	17.745021839319534
6	24.34613405729674	31.639700489332125	29.137538451433514	14.876627001937623
7	75.26223603980338	5.228738136159126	14.89835685119706	4.6106689728404255
8	76.39990585556492	4.71866139749751	14.246242432867353	4.635190314070215
9	70.34061675552003	6.678288760687036	16.137834021171553	6.843260462621376
10	35.73793911263397	27.617817381690003	22.66987049666663	13.974373009009403
11	25.479808208081096	24.131736526946106	31.668272230675758	18.720183034297037
12	22.673701956233785	21.66690385225892	34.94367754436283	20.71571664714447
13	21.933682908406222	22.627779176564605	36.49240823654337	18.946129678485807
14	19.12336205103504	25.776746324535573	34.924246570843685	20.1756450535857
15	18.33621605053148	24.963820074658727	37.060285279532344	19.639678595277452
16	21.624374651063505	25.830167817929038	33.50480027148628	19.040657259521176
17	21.836309100263822	26.03022474247118	31.99088112623017	20.142585031034823
18	22.99948548971527	24.769565074603992	32.52947487109875	19.70147456458199
19	21.59454400157637	27.48360682656625	30.57225585392287	20.349593317934513
20	23.443770594095174	26.705273183067142	30.92557116115119	18.925385061686498
21	22.293128550941994	27.032698770648828	32.5233445357913	18.150828142617872
22	22.494225443080932	23.16150149426923	32.80172744091342	21.542545621736416
23	21.14664637817601	25.747134615595137	32.61349330589278	20.492725700336074
24	20.9693592704901	24.224731524154617	33.571139257134725	21.23476994822056
25	21.323057723675138	26.84435516535485	31.204118271683324	20.628468839286693
26	20.89382478188048	26.001379325444173	31.000886709214114	22.10390918346123
27	21.293555485008046	24.97498604254015	31.22404186143253	22.507416611019277
28	19.508314267260726	26.315668480224197	31.412330731589837	22.76368652092524
29	20.770451783818103	26.336467832160178	30.33777052841301	22.55530985560871
30	22.071889128507156	25.61839757413874	30.66289724025441	21.646816057099695
31	22.89537925976201	25.321459457684263	28.987071560717688	22.796089721836037
32	21.997832488587722	26.238218261830998	28.774918170970672	22.9890310786106
33	21.630942867464338	25.69453414925177	28.794075468806447	23.880447514477442
34	19.56272099311432	26.36509430864049	30.576744135130106	23.495440563115086
35	19.694687407634458	26.680095020197264	30.41412604407273	23.211091528095544
36	20.610187303637698	28.09505303834744	29.391235809915816	21.90352384809905
37	20.371432637467297	26.235590975270668	30.073126142595978	23.31985024466606
38	19.861739044762395	27.40331038106602	29.753472944421944	22.98147762974964
39	20.45063438023405	25.665798202498113	29.590471707407858	24.293095709859987
40	22.096684145420312	24.836560881892524	31.180746368323682	21.886008604363486
41	19.855991855411663	25.615387141621692	30.90931482555912	23.619306177407527
42	21.609213018204905	25.389385762296246	30.8670045649104	22.134396654588446
43	21.459786095085878	24.84438800643685	30.522994230916595	23.172831667560672
44	22.047805668370753	24.626213751655737	28.96052501943097	24.365455560542536
45	21.554149470711227	24.04525501100176	28.93414268355428	25.466452834732728
46	21.688469496108333	24.69233379675749	28.936715234977946	24.682481472156237
47	20.065408488324994	25.16431488029426	31.212219071911022	23.558057559469724
48	20.387032151419284	24.893102278076388	31.097111079486368	23.622754491017965
49	21.463562819516362	23.61498210161031	31.025134374760533	23.896320704112796
50	19.88106054800819	23.95176739756319	31.988363309943185	24.178808744485437
51	20.015763719362006	23.264622491762363	30.291026721693726	26.42858706718191
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	897.0
1	1468.5
2	2040.0
3	19162.5
4	36285.0
5	24334.0
6	12383.0
7	12362.5
8	12342.0
9	12374.5
10	12407.0
11	12274.5
12	12142.0
13	11669.0
14	11196.0
15	10932.0
16	10668.0
17	10283.0
18	9898.0
19	9584.0
20	9270.0
21	9290.5
22	9311.0
23	9331.0
24	9351.0
25	10686.0
26	13477.5
27	14934.0
28	18197.5
29	21461.0
30	25202.0
31	28943.0
32	32144.5
33	35346.0
34	41302.0
35	47258.0
36	51671.0
37	56084.0
38	61796.0
39	67508.0
40	73365.5
41	79223.0
42	85538.0
43	91853.0
44	101061.0
45	110269.0
46	138550.5
47	166832.0
48	171493.0
49	176154.0
50	173427.0
51	170700.0
52	151068.0
53	131436.0
54	117719.0
55	104002.0
56	95346.0
57	86690.0
58	81194.0
59	75698.0
60	67968.5
61	60239.0
62	52306.0
63	44373.0
64	38491.5
65	32610.0
66	26973.0
67	21336.0
68	18344.0
69	15352.0
70	12747.5
71	10143.0
72	9277.5
73	8412.0
74	6692.0
75	3779.5
76	2587.0
77	1848.5
78	1110.0
79	909.0
80	708.0
81	513.0
82	318.0
83	206.0
84	94.0
85	74.5
86	55.0
87	43.0
88	31.0
89	26.5
90	22.0
91	17.0
92	12.0
93	6.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1826980.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.742682996492352
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.6354397089672	21.901155405711364
2	10.019895527522792	5.960371526661638
3	3.693942850701163	3.296033136466919
4	2.0807785967807297	2.4755175275974173
5	1.2578731545612745	1.8706261242956908
6	0.907997393907736	1.6203767189183396
7	0.7395810074628626	1.5397986418636056
8	0.5726962192174078	1.362681768117844
9	0.5053730424180104	1.3528035176050592
>10	5.931555142614907	39.61529885759782
>50	0.6007809279480205	11.150571027117461
>100	0.0472783618165682	2.6160080509142163
>500	0.003404033040686206	0.6783420067793957
>1k	0.002647581253867049	2.162144837529019
>5k	5.673388401143676E-4	1.196210568671427
>10k+	1.891129467047892E-4	1.202060284152775
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21371	1.169744605852281	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTT	8706	0.4765240998806774	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTC	6338	0.3469112962375067	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTC	6223	0.34061675552003856	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCT	4961	0.27154101303790956	No Hit
CGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG	4836	0.264699120953705	No Hit
CGTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCT	4177	0.2286286658857787	No Hit
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC	3324	0.18193959430316697	TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC	2854	0.15621408006655793	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGC	2850	0.15599513951986338	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCG	2721	0.1489343068889643	No Hit
CTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGCT	2294	0.1255624035293216	TruSeq Adapter, Index 16 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCC	2072	0.11341120318777435	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTC	2059	0.1126996464110171	No Hit
GAACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCT	2020	0.11056497608074528	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6420541002090883E-4	0.0	0.0	0.1341558199870825	0.0
2	1.6420541002090883E-4	0.0	0.0	0.5752115513032436	0.0
3	1.6420541002090883E-4	0.0	0.0	1.0568807540312428	0.0
4	1.6420541002090883E-4	0.0	0.0	1.7034121884202345	0.0
5	1.6420541002090883E-4	0.0	0.0	3.2215459392002104	0.0
6	1.6420541002090883E-4	0.0	0.0	4.750681452451587	0.0
7	1.6420541002090883E-4	0.0	0.0	5.851952402325149	0.0
8	1.6420541002090883E-4	0.0	0.0	7.253117166033563	0.0
9	1.6420541002090883E-4	0.0	0.0	8.024937328268509	0.0
10	1.6420541002090883E-4	0.0	0.0	9.252920119541539	0.0
11	2.189405466945451E-4	0.0	0.0	10.373731513207588	0.0
12	2.7367568336818135E-4	0.0	0.0	11.416654807387054	0.0
13	2.7367568336818135E-4	0.0	0.0	11.890661090980744	0.0
14	2.7367568336818135E-4	0.0	0.0	12.14933934690035	0.0
15	2.7367568336818135E-4	0.0	0.0	12.446441668764846	0.0
16	2.7367568336818135E-4	0.0	0.0	12.912894503497576	0.0
17	2.7367568336818135E-4	0.0	0.0	13.453075567329691	0.0
18	2.7367568336818135E-4	0.0	0.0	14.098129153028495	0.0
19	3.2841082004181766E-4	0.0	0.0	14.52391378121271	0.0
20	3.2841082004181766E-4	0.0	0.0	14.964422161162137	0.0
21	3.831459567154539E-4	0.0	0.0	15.458735180461746	0.0
22	3.831459567154539E-4	0.0	0.0	16.015500990705974	0.0
23	3.831459567154539E-4	0.0	0.0	16.57478461723719	0.0
24	3.831459567154539E-4	0.0	0.0	17.010914186252723	0.0
25	4.378810933890902E-4	0.0	0.0	17.42296029513186	0.0
26	4.378810933890902E-4	0.0	0.0	17.80670833835072	0.0
27	4.378810933890902E-4	0.0	0.0	18.224009020350525	0.0
28	4.378810933890902E-4	0.0	0.0	18.6583870649925	0.0
29	4.378810933890902E-4	0.0	0.0	19.10152273150226	0.0
30	4.378810933890902E-4	0.0	0.0	19.686860283090127	0.0
31	4.926162300627264E-4	0.0	0.0	20.153805734052916	0.0
32	5.473513667363627E-4	0.0	0.0	20.630001423113555	0.0
33	5.473513667363627E-4	0.0	0.0	21.095906906479545	0.0
34	6.020865034099991E-4	0.0	0.0	21.572321536086875	0.0
35	6.020865034099991E-4	0.0	0.0	22.052841300944728	0.0
36	6.020865034099991E-4	0.0	0.0	22.484318383343002	0.0
37	6.020865034099991E-4	0.0	0.0	22.94666608282521	0.0
38	6.020865034099991E-4	0.0	0.0	23.414104150018062	0.0
39	7.662919134309078E-4	0.0	0.0	24.0125781344076	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCGA	20	7.034381E-4	45.0	18
CGTTTTT	13800	0.0	43.858692	1
CGACGGT	90	0.0	42.500004	28
TAACGCG	85	0.0	42.35294	1
CGTTTCT	1085	0.0	40.02304	1
CCGGTAT	45	1.929402E-8	40.000004	42
CGTTATT	330	0.0	39.545452	1
TCCGATC	40	3.4597178E-7	39.375	14
GCTATCG	40	3.4597178E-7	39.375	1
CGAATAT	230	0.0	39.130436	14
TTGTACG	75	0.0	39.0	1
TTACGCG	75	0.0	39.0	1
TATAGCG	145	0.0	38.793102	1
CACGACC	425	0.0	38.64706	27
CGTAGCG	35	6.250313E-6	38.571426	2
CGTTCTG	765	0.0	38.52941	1
AACGAGC	995	0.0	38.44221	15
ACAACGA	1010	0.0	38.31683	13
CGGTCTA	100	0.0	38.250004	31
CGTTTTC	720	0.0	38.125004	1
>>END_MODULE