FastQCFastQC Report
Sat 14 Jan 2017
SRR2935796.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935796.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1047685
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTC247572.3630194190047584No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155661.485751919708691No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGC136701.3047814944377365No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCG136571.3035406634627775No Hit
GAACTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCT65800.6280513704023634No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTT53280.5085498026601507No Hit
GCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC45500.43429084123567674No Hit
CGTTCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTC37530.35821835761703186No Hit
GAATGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTT33930.32385688446431893No Hit
CGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG31000.29589046325947205No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCT28470.27174198351603773No Hit
GAATGACTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCT27630.2637243064470714No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTC24500.23384891451151824No Hit
ACCATACGGAAAGAGTTCCGAACCCGCATGGTTATGAACAATGGACTCAAC22890.21848170012933277No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACACAAAT19540.18650644039000272No Hit
CGTCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCT18770.17915690307678359No Hit
CCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC16260.15519932040641987No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGACACAAATCGTA15880.15157227601807796No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCC12580.12007425896142447No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTC12010.11463369237891159No Hit
CTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCT10930.10432525043309773No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCGT207.0330006E-445.042
CTTATCG207.0330006E-445.041
CGTTTTT76150.043.729481
TACGGCT16850.041.795257
CGATGAA9350.040.18716419
AACCCGC3550.039.92957721
CCATACG3450.039.7826082
AACGGGC1250.039.64
CCGATGA9400.039.49468218
GAATCTG29400.039.107141
ACGGCTG18400.038.7635848
TCCGAAC3600.038.7517
CCGCATG3750.038.424
CGTTAGG1350.038.3333362
CCCGCAT3700.038.3108123
ATACGGA3650.038.2191774
AATCTGT30100.038.1976742
GTTTTTT88850.038.1879582
ACCATAC3600.038.1251
CATACGA659.094947E-1238.07692718
Produced by FastQC (version 0.11.5)