##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935796.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1047685
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.970301187857036	33.0	31.0	34.0	30.0	34.0
2	32.06443921598572	33.0	31.0	34.0	30.0	34.0
3	32.01162944969146	33.0	31.0	34.0	30.0	34.0
4	35.737992812725196	37.0	35.0	37.0	35.0	37.0
5	34.80016989839503	37.0	35.0	37.0	33.0	37.0
6	35.23912912755265	37.0	35.0	37.0	33.0	37.0
7	35.749867565155554	37.0	35.0	37.0	33.0	37.0
8	35.67649627512086	37.0	35.0	37.0	33.0	37.0
9	37.57341376463345	39.0	37.0	39.0	35.0	39.0
10	37.3344058567222	39.0	37.0	39.0	34.0	39.0
11	37.22946687219918	39.0	37.0	39.0	34.0	39.0
12	37.115065119764054	39.0	37.0	39.0	34.0	39.0
13	37.00450230746837	39.0	37.0	39.0	33.0	39.0
14	38.083164309883216	40.0	37.0	41.0	33.0	41.0
15	38.11095319681011	40.0	37.0	41.0	33.0	41.0
16	38.22137188181562	40.0	37.0	41.0	33.0	41.0
17	38.12908364632499	40.0	37.0	41.0	33.0	41.0
18	37.998114891403425	40.0	37.0	41.0	33.0	41.0
19	37.850188749481	40.0	37.0	41.0	33.0	41.0
20	37.61176021418652	40.0	35.0	41.0	33.0	41.0
21	37.63354443367997	39.0	35.0	41.0	33.0	41.0
22	37.6793740484974	39.0	35.0	41.0	33.0	41.0
23	37.6854512568186	39.0	35.0	41.0	33.0	41.0
24	37.60915065119764	39.0	35.0	41.0	33.0	41.0
25	37.51877902232064	39.0	35.0	41.0	33.0	41.0
26	37.459939771973445	39.0	35.0	41.0	33.0	41.0
27	37.453126655435554	39.0	35.0	41.0	33.0	41.0
28	37.28634370063521	39.0	35.0	41.0	33.0	41.0
29	37.16992321165236	39.0	35.0	41.0	32.0	41.0
30	37.015640197196674	39.0	35.0	41.0	32.0	41.0
31	36.91945861590077	39.0	35.0	41.0	31.0	41.0
32	36.60834029312245	39.0	35.0	41.0	31.0	41.0
33	36.321402902589995	39.0	35.0	41.0	30.0	41.0
34	36.04053794795191	39.0	35.0	41.0	29.0	41.0
35	35.83893250356739	39.0	35.0	41.0	27.0	41.0
36	35.7879610760868	39.0	35.0	41.0	27.0	41.0
37	35.65610369529009	39.0	35.0	41.0	26.0	41.0
38	35.6132730734906	39.0	35.0	41.0	26.0	41.0
39	35.45044455155891	39.0	35.0	41.0	25.0	41.0
40	35.23928661763794	38.0	35.0	41.0	24.0	41.0
41	35.02786715472685	38.0	34.0	40.0	23.0	41.0
42	35.107137164319425	38.0	35.0	40.0	23.0	41.0
43	35.05669070378978	38.0	35.0	40.0	23.0	41.0
44	35.00420069009292	38.0	35.0	40.0	23.0	41.0
45	34.95367691624868	38.0	34.0	40.0	23.0	41.0
46	34.834985706581655	38.0	34.0	40.0	23.0	41.0
47	34.60356786629569	37.0	34.0	40.0	23.0	41.0
48	34.617405040637216	37.0	34.0	40.0	23.0	41.0
49	34.63122503424216	37.0	34.0	40.0	23.0	41.0
50	34.5192238125009	37.0	34.0	40.0	23.0	41.0
51	33.369193030347866	36.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	9.0
9	25.0
10	31.0
11	31.0
12	25.0
13	24.0
14	39.0
15	59.0
16	113.0
17	232.0
18	362.0
19	889.0
20	1667.0
21	2852.0
22	4306.0
23	6045.0
24	8393.0
25	12325.0
26	16624.0
27	19009.0
28	18825.0
29	18336.0
30	19874.0
31	23916.0
32	30543.0
33	41277.0
34	68615.0
35	78422.0
36	79529.0
37	111354.0
38	183725.0
39	299998.0
40	207.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.848198647494236	18.255868891890216	19.40573741153114	18.49019504908441
2	35.733450416871484	28.92481996019796	20.11692445725576	15.224805165674798
3	26.761383431088543	28.528994879186016	29.685640244920947	15.023981444804496
4	24.21185757169378	21.233385989109323	37.8295957277235	16.725160711473393
5	26.83010637739397	25.980423505156608	28.60344473768356	18.586025379765864
6	22.904880760915734	33.878408109307664	29.49187971575426	13.724831414022345
7	68.97550313309821	4.931348640096975	20.975197697781297	5.117950529023513
8	68.59094097939743	9.325512916573206	16.70740728367782	5.376138820351537
9	62.899917437015894	5.8073753084180835	17.62991738929163	13.66278986527439
10	39.60207505118428	19.318688346210934	25.024506411755443	16.054730190849348
11	29.99613433427032	21.861437359511683	29.121634842533776	19.020793463684218
12	25.095901917083857	18.785035578441995	33.73599889279698	22.383063611677173
13	24.513188601535767	19.07901707097076	38.13379021366155	18.274004113831925
14	19.30303478621914	26.038265318297004	33.9074244644144	20.751275431069452
15	17.6622744431771	22.352520079985876	39.41280060323475	20.572404873602277
16	21.127724459164728	23.80333783532264	31.92343118399137	23.14550652152126
17	21.529467349441862	24.343385655039445	32.43207643518806	21.695070560330635
18	21.22345934130965	23.87606962016255	32.08149396049385	22.81897707803395
19	21.103766876494365	25.213971756778044	30.168705288326166	23.51355607840143
20	22.73326429222524	25.84536382595914	32.66019843750746	18.761173444308167
21	22.21841488615376	26.999909323890293	32.98290993953335	17.798765850422598
22	21.288268897617126	21.158936130611778	34.25103919594153	23.301755775829566
23	21.449099681679133	23.863088619193746	33.70345094183843	20.98436075728869
24	22.959763669423538	23.39959052577826	31.06802139956189	22.572624405236308
25	22.09375909743864	28.134887871831705	28.615566701823543	21.15578632890611
26	20.947231276576453	22.906407937500298	31.343294978929737	24.803065806993516
27	23.59478278299298	23.31836382118671	29.929415807232136	23.157437588588174
28	20.625951502598586	23.651001971012278	31.870170900604666	23.852875625784467
29	24.794666335778405	23.098068598863208	29.82480421118943	22.282460854168953
30	22.3601559629087	26.826574781542163	29.99307998110119	20.82018927444795
31	23.152092470542197	23.964741310603856	27.438209003660454	25.444957215193497
32	24.07078463469459	28.199601979602644	26.601888926538038	21.127724459164728
33	22.050902704534284	25.27649054820867	26.324801824976017	26.347804922281032
34	22.575392412795832	27.90180254561247	27.42704152488582	22.09576351670588
35	20.324525024220065	31.469000701546747	26.26104220257043	21.94543207166276
36	22.683249259080736	30.154865250528545	25.227334551892984	21.934550938497736
37	20.053069386313634	30.743496375341827	28.23148179080544	20.9719524475391
38	20.870395204665524	26.77751423376301	25.799166734276046	26.552923827295416
39	22.80189178999413	27.867250175386687	26.601507132391895	22.729350902227292
40	21.860196528536726	25.83085564840577	29.720765306365944	22.588182516691564
41	19.92526379589285	29.6641643242005	27.825348267847684	22.585223612058968
42	20.713955053284145	26.352004657888585	31.335181853324233	21.598858435503036
43	22.835966917537238	25.170733569727545	28.365491536101022	23.627807976634198
44	22.664064103237138	22.87557806019939	29.3330533509595	25.127304485603975
45	21.154163703785013	22.323121930733	28.69307091349022	27.82964345199177
46	24.89479185060395	24.38605115087073	27.743262526427316	22.97589447209801
47	20.11043395677136	23.60805012957139	33.822188921288365	22.45932699236889
48	20.79346368421806	25.110505543173762	29.17670864811465	24.919322124493526
49	20.46311629926934	22.876914339710886	33.4219732075958	23.23799615342398
50	20.66985782940483	22.64669246958771	32.0548638188005	24.62858588220696
51	20.264583343275888	22.703866142972362	29.60508168008514	27.426468833666608
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	425.0
1	891.5
2	1358.0
3	13497.5
4	25637.0
5	16817.0
6	7997.0
7	7672.0
8	7347.0
9	7238.5
10	7130.0
11	6924.0
12	6718.0
13	6434.0
14	6150.0
15	5809.5
16	5469.0
17	5157.0
18	4845.0
19	4645.0
20	4445.0
21	4411.0
22	4377.0
23	4484.0
24	4591.0
25	5037.5
26	6196.0
27	6908.0
28	8422.0
29	9936.0
30	11072.5
31	12209.0
32	13852.0
33	15495.0
34	18476.5
35	21458.0
36	22861.5
37	24265.0
38	27114.5
39	29964.0
40	33409.5
41	36855.0
42	42724.0
43	48593.0
44	93203.0
45	137813.0
46	112272.5
47	86732.0
48	87283.5
49	87835.0
50	78056.0
51	68277.0
52	63078.0
53	57879.0
54	54997.0
55	52115.0
56	51107.0
57	50099.0
58	47783.0
59	45467.0
60	42823.5
61	40180.0
62	37434.5
63	34689.0
64	31382.5
65	28076.0
66	24087.0
67	20098.0
68	17705.0
69	15312.0
70	12929.5
71	10547.0
72	8410.5
73	6274.0
74	5272.5
75	3192.5
76	2114.0
77	1643.5
78	1173.0
79	858.0
80	543.0
81	426.0
82	309.0
83	211.5
84	114.0
85	85.0
86	56.0
87	39.0
88	22.0
89	14.5
90	7.0
91	6.0
92	5.0
93	6.0
94	7.0
95	11.0
96	15.0
97	7.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1047685.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.457938734090206
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.24961404902135	24.84239037882
2	7.962978991191301	5.32849726456252
3	3.355655928008667	3.368199913560018
4	1.882190592475961	2.518968701157666
5	1.440394128543016	2.409630925286774
6	1.1848483937806005	2.3785550980977908
7	1.0157095203457387	2.3788482832351914
8	0.9240157208720453	2.4732529102618472
9	0.8526212217697335	2.5674253741221196
>10	7.046443162110877	37.30509685349696
>50	0.05103215949539639	1.1348187903009657
>100	0.02622846429375003	1.8054644620454732
>500	0.002280736025245704	0.4908558812898828
>1k	0.004276380047335696	3.4092831148587237
>5k	5.70184006311426E-4	1.135855389506398
>10k+	0.001140368012622852	6.4528566593977015
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTC	24757	2.3630194190047584	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15566	1.485751919708691	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGC	13670	1.3047814944377365	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCG	13657	1.3035406634627775	No Hit
GAACTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCT	6580	0.6280513704023634	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTT	5328	0.5085498026601507	No Hit
GCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC	4550	0.43429084123567674	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTC	3753	0.35821835761703186	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTT	3393	0.32385688446431893	No Hit
CGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG	3100	0.29589046325947205	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCT	2847	0.27174198351603773	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCT	2763	0.2637243064470714	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTC	2450	0.23384891451151824	No Hit
ACCATACGGAAAGAGTTCCGAACCCGCATGGTTATGAACAATGGACTCAAC	2289	0.21848170012933277	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACACAAAT	1954	0.18650644039000272	No Hit
CGTCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCT	1877	0.17915690307678359	No Hit
CCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC	1626	0.15519932040641987	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGACACAAATCGTA	1588	0.15157227601807796	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCC	1258	0.12007425896142447	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTC	1201	0.11463369237891159	No Hit
CTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCT	1093	0.10432525043309773	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.817941461412543E-4	0.0	0.0	0.11091119945403437	0.0
2	3.817941461412543E-4	0.0	0.0	0.7815326171511475	0.0
3	3.817941461412543E-4	0.0	0.0	1.283019228107685	0.0
4	3.817941461412543E-4	0.0	0.0	2.3382027995055767	0.0
5	3.817941461412543E-4	0.0	0.0	5.7758772913614305	0.0
6	3.817941461412543E-4	0.0	0.0	7.472475028276629	0.0
7	5.726912192118815E-4	0.0	0.0	8.629788533767305	0.0
8	5.726912192118815E-4	0.0	0.0	9.84828455117712	0.0
9	5.726912192118815E-4	0.0	0.0	10.463450369147216	0.0
10	6.68139755747195E-4	0.0	0.0	12.643781289223384	0.0
11	6.68139755747195E-4	0.0	0.0	13.506731508039152	0.0
12	6.68139755747195E-4	0.0	0.0	15.513727885767192	0.0
13	6.68139755747195E-4	0.0	0.0	15.903253363367806	0.0
14	6.68139755747195E-4	0.0	0.0	16.151324109823086	0.0
15	6.68139755747195E-4	0.0	0.0	16.50620176866138	0.0
16	6.68139755747195E-4	0.0	0.0	16.874633119687694	0.0
17	6.68139755747195E-4	0.0	0.0	17.233901411206613	0.0
18	6.68139755747195E-4	0.0	0.0	17.61970439588235	0.0
19	6.68139755747195E-4	0.0	0.0	18.078239165397996	0.0
20	6.68139755747195E-4	0.0	0.0	18.370311687196057	0.0
21	6.68139755747195E-4	0.0	0.0	18.670974577282294	0.0
22	6.68139755747195E-4	0.0	0.0	19.0761536148747	0.0
23	6.68139755747195E-4	0.0	0.0	19.396669800560282	0.0
24	6.68139755747195E-4	0.0	0.0	19.694373786013927	0.0
25	6.68139755747195E-4	0.0	0.0	19.960770651483987	0.0
26	6.68139755747195E-4	0.0	0.0	20.216954523544768	0.0
27	6.68139755747195E-4	0.0	0.0	20.525825987773043	0.0
28	6.68139755747195E-4	0.0	0.0	20.782009859833824	0.0
29	6.68139755747195E-4	0.0	0.0	21.066351050172525	0.0
30	6.68139755747195E-4	0.0	0.0	21.42838734925097	0.0
31	7.635882922825086E-4	0.0	0.0	21.7030882373996	0.0
32	7.635882922825086E-4	0.0	0.0	21.98151161847311	0.0
33	7.635882922825086E-4	0.0	0.0	22.26260755856961	0.0
34	7.635882922825086E-4	0.0	0.0	22.56202961768089	0.0
35	7.635882922825086E-4	0.0	0.0	22.86116533118256	0.0
36	7.635882922825086E-4	0.0	0.0	23.153715095663294	0.0
37	8.590368288178222E-4	0.0	0.0	23.466595398426055	0.0
38	8.590368288178222E-4	0.0	0.0	23.84896223578652	0.0
39	8.590368288178222E-4	0.0	0.0	24.364766127223355	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATCGT	20	7.0330006E-4	45.0	42
CTTATCG	20	7.0330006E-4	45.0	41
CGTTTTT	7615	0.0	43.72948	1
TACGGCT	1685	0.0	41.79525	7
CGATGAA	935	0.0	40.187164	19
AACCCGC	355	0.0	39.929577	21
CCATACG	345	0.0	39.782608	2
AACGGGC	125	0.0	39.6	4
CCGATGA	940	0.0	39.494682	18
GAATCTG	2940	0.0	39.10714	1
ACGGCTG	1840	0.0	38.763584	8
TCCGAAC	360	0.0	38.75	17
CCGCATG	375	0.0	38.4	24
CGTTAGG	135	0.0	38.333336	2
CCCGCAT	370	0.0	38.31081	23
ATACGGA	365	0.0	38.219177	4
AATCTGT	3010	0.0	38.197674	2
GTTTTTT	8885	0.0	38.187958	2
ACCATAC	360	0.0	38.125	1
CATACGA	65	9.094947E-12	38.076927	18
>>END_MODULE