FastQCFastQC Report
Sat 14 Jan 2017
SRR2935795.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935795.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1276954
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124050.9714523780809645No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTT36640.28693281042230184No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTC31950.2502047841973947No Hit
CGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG30140.2360304286607035No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTC27980.21911517564454164No Hit
CCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC27890.21841037343553488Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC22730.17800171345248145Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCT21680.16977902101406941No Hit
CTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT17440.13657500583419607Illumina Single End Adapter 2 (95% over 22bp)
CGTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCT16570.1297619178137975No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCG14770.11566587363366261No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGC14300.11198523987551627No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCC13010.1018830748797529No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG253.891051E-544.9999961
CGTTTTT71800.043.4018141
CGGTCTA603.6379788E-1241.25000431
CTCGTAG501.0822987E-940.4999961
TCGATAG403.4586265E-739.3751
TTGCGAG1150.039.1304321
CGTTCTG4350.036.2068941
GCGCGAC2800.036.1607139
ACAACGA3050.036.1475413
TTAACGG751.8189894E-1236.02
CGTAAGG2250.036.02
ACTGCGC250.002107196535.99999627
GTCGAGT250.002107196535.99999614
TAGACGG1000.035.9999962
CGTCCCG250.002107196535.99999626
GCGTAGT250.002107196535.99999639
GTTTTTT89550.035.728642
TACGGGA5150.035.388354
TTGTACG702.1827873E-1135.3571431
GTATGCG702.1827873E-1135.3571431
Produced by FastQC (version 0.11.5)