##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935795.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1276954
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10516510383303	33.0	31.0	34.0	30.0	34.0
2	32.17483715153404	33.0	31.0	34.0	30.0	34.0
3	32.13999407966144	33.0	31.0	34.0	30.0	34.0
4	35.821919192077395	37.0	35.0	37.0	35.0	37.0
5	34.92179749622931	37.0	35.0	37.0	33.0	37.0
6	35.387415678246825	37.0	35.0	37.0	33.0	37.0
7	35.962388621673135	37.0	35.0	37.0	35.0	37.0
8	35.96589853667399	37.0	35.0	37.0	35.0	37.0
9	37.83753369346116	39.0	38.0	39.0	35.0	39.0
10	37.45690134491924	39.0	37.0	39.0	35.0	39.0
11	37.38232309072997	39.0	37.0	39.0	35.0	39.0
12	37.274836838288614	39.0	37.0	39.0	34.0	39.0
13	37.21268033147631	39.0	37.0	39.0	34.0	39.0
14	38.24335567295298	40.0	38.0	41.0	33.0	41.0
15	38.39217857495258	40.0	38.0	41.0	34.0	41.0
16	38.41049716747823	40.0	38.0	41.0	34.0	41.0
17	38.398336196918606	40.0	38.0	41.0	34.0	41.0
18	38.32520826905276	40.0	38.0	41.0	34.0	41.0
19	38.1680044856745	40.0	37.0	41.0	34.0	41.0
20	38.11103532312049	40.0	36.0	41.0	34.0	41.0
21	38.0291169454812	40.0	36.0	41.0	34.0	41.0
22	38.08494432845662	40.0	36.0	41.0	34.0	41.0
23	38.095337811698776	40.0	36.0	41.0	34.0	41.0
24	38.055329322747724	40.0	36.0	41.0	34.0	41.0
25	37.89826023490274	40.0	35.0	41.0	34.0	41.0
26	37.91701580479798	40.0	36.0	41.0	34.0	41.0
27	37.92469736576259	40.0	36.0	41.0	34.0	41.0
28	37.81203786510712	40.0	35.0	41.0	34.0	41.0
29	37.75667095290825	40.0	35.0	41.0	34.0	41.0
30	37.60112580406185	40.0	35.0	41.0	33.0	41.0
31	37.49107407157971	40.0	35.0	41.0	33.0	41.0
32	37.3266946186002	40.0	35.0	41.0	33.0	41.0
33	37.07323521442432	40.0	35.0	41.0	32.0	41.0
34	36.85347475320176	40.0	35.0	41.0	31.0	41.0
35	36.722657981415146	40.0	35.0	41.0	31.0	41.0
36	36.610651597473364	40.0	35.0	41.0	31.0	41.0
37	36.56527094946255	40.0	35.0	41.0	30.0	41.0
38	36.46840919876519	39.0	35.0	41.0	30.0	41.0
39	36.39149256746915	39.0	35.0	41.0	30.0	41.0
40	36.262707975385176	39.0	35.0	41.0	30.0	41.0
41	36.140866468173485	39.0	35.0	41.0	29.0	41.0
42	36.15440885106276	39.0	35.0	41.0	30.0	41.0
43	36.064960836490584	39.0	35.0	41.0	29.0	41.0
44	35.947404526709654	39.0	35.0	41.0	29.0	41.0
45	35.91032801494807	39.0	35.0	41.0	29.0	41.0
46	35.797427315314415	39.0	35.0	41.0	29.0	41.0
47	35.6848461260155	39.0	35.0	41.0	28.0	41.0
48	35.6299733584765	38.0	35.0	41.0	28.0	41.0
49	35.59505432458804	38.0	35.0	40.0	28.0	41.0
50	35.48147309926591	38.0	35.0	40.0	27.0	41.0
51	34.3660037871372	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	25.0
9	22.0
10	34.0
11	32.0
12	26.0
13	28.0
14	36.0
15	66.0
16	104.0
17	218.0
18	351.0
19	660.0
20	1240.0
21	1950.0
22	3019.0
23	4494.0
24	6859.0
25	11198.0
26	15802.0
27	17782.0
28	17701.0
29	18152.0
30	19630.0
31	23573.0
32	30754.0
33	43263.0
34	79213.0
35	90251.0
36	87644.0
37	132495.0
38	239025.0
39	431019.0
40	286.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.20786966484305	20.549526451226903	23.505388604444637	15.737215279485401
2	35.60652928766424	23.39324674185601	24.383572156867046	16.616651813612705
3	30.69617229751424	22.924240027440298	30.337506284486366	16.042081390559098
4	27.002068986040218	24.57825418926602	30.446124136029955	17.973552688663805
5	24.00384038892552	29.7572974437607	28.395854510029334	17.843007657284442
6	24.8257963873405	32.752393586613145	28.062326442456033	14.359483583590324
7	77.59292817125754	5.202536661461572	12.549943067643785	4.654592099637105
8	79.23347277975557	4.613322014731932	11.77152818347411	4.381677022038382
9	73.88981905377953	6.13851399502253	13.735107137766905	6.2365598134310245
10	38.46364082026447	25.717762738516814	21.152367273997342	14.666229167221372
11	28.658040931779844	24.754454741517705	27.96529867168277	18.62220565501968
12	25.608831641546992	21.569375247659664	32.91582938774616	19.905963723047186
13	24.252400634635233	22.12945806975036	34.33287338463249	19.285267910981915
14	19.98075106855846	25.34468743588258	34.47477356271252	20.199787932846444
15	19.499762716589633	24.6320540912202	36.19433432997587	19.6738488622143
16	23.95301631852048	24.514273810959516	32.38519163572063	19.147518234799374
17	23.99428640342565	24.54794769427873	30.227165583098532	21.23060031919709
18	24.71428101560432	24.066332851457453	31.262833273555664	19.956552859382562
19	23.303658549955596	26.688901871171556	29.638029247725445	20.369410331147403
20	23.4918407397604	26.896348654689206	30.122150053956524	19.48966055159387
21	23.49176242840384	26.19718486335451	31.80913329689245	18.501919411349196
22	22.167439077680168	23.67156530305712	32.276730406890145	21.88426521237257
23	20.821971660686287	25.644698242849785	32.18001588154311	21.353314214920818
24	21.65786708056829	24.77426751472645	31.84640950261325	21.72145590209201
25	22.640283048567138	25.87955400116214	29.729340289470095	21.750822660800623
26	21.144066270202373	25.267863995100843	30.208997348377466	23.37907238631932
27	21.417999395436325	24.58522389999953	31.532067717396238	22.4647089871679
28	20.026249966717675	25.21469058399911	31.419221052598605	23.33983839668461
29	21.09895892882594	25.594500663297193	30.47556920609513	22.83097120178174
30	22.993388955279517	23.929287977483916	31.10354797432014	21.973775092916426
31	23.661306515348244	24.249581425799207	29.29557368550472	22.793538373347825
32	23.677203720729175	24.731822759472934	29.00629153438573	22.58468198541216
33	23.559345129111932	24.37644582342042	27.958877140445153	24.105331907022492
34	20.767153711096874	25.742117570405824	30.13475818236209	23.35597053613521
35	21.989202430158016	26.753665363043616	28.400944748205497	22.85618745859287
36	23.15267425451504	27.91353486499905	26.8571146650545	22.07667621543141
37	21.917390916195885	28.51590581963015	27.13465011269004	22.43205315148392
38	22.103458699373665	27.027050308781682	27.296597998048483	23.572892993796174
39	21.721377590735454	26.26570730034128	27.23034659040185	24.78256851852142
40	24.037905829027515	24.984220261653906	28.750918200655622	22.22695570866296
41	21.5089188803982	26.383800826028185	28.325452600485217	23.7818276930884
42	21.643301168248815	26.120048177146554	29.090006374544426	23.146644280060205
43	22.16172234865156	25.16073405933182	29.2058288708912	23.471714721125426
44	22.394463700336896	24.744509199235054	28.51018909060154	24.350838009826507
45	21.635704966662857	23.987238381335587	28.277682672985872	26.099373979015688
46	22.626108693030446	24.547164580713165	28.350042366443894	24.47668435981249
47	21.213215198041592	25.56286287524844	30.092313427108575	23.131608499601395
48	20.981883450774262	25.790670611470734	29.324940444213336	23.90250549354166
49	22.25319001310932	24.277930136872587	29.71978630397023	23.74909354604786
50	20.401987855474825	24.486943147521366	30.442835059054595	24.668233937949214
51	20.384994291102107	23.904306654742456	29.318362290262613	26.392336763892825
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	687.0
1	1170.0
2	1653.0
3	11085.0
4	20517.0
5	13594.0
6	6671.0
7	6548.5
8	6426.0
9	6389.0
10	6352.0
11	6307.0
12	6262.0
13	6277.0
14	6292.0
15	5854.0
16	5416.0
17	5325.0
18	5234.0
19	5067.5
20	4901.0
21	5030.5
22	5160.0
23	5405.0
24	5650.0
25	6790.0
26	9309.5
27	10689.0
28	11687.0
29	12685.0
30	15981.5
31	19278.0
32	22221.5
33	25165.0
34	29009.5
35	32854.0
36	35621.5
37	38389.0
38	42373.0
39	46357.0
40	50078.5
41	53800.0
42	58079.5
43	62359.0
44	66215.5
45	70072.0
46	76107.5
47	82143.0
48	98557.0
49	114971.0
50	119239.0
51	123507.0
52	120166.5
53	116826.0
54	102056.0
55	87286.0
56	79404.0
57	71522.0
58	65747.5
59	59973.0
60	52839.5
61	45706.0
62	40694.5
63	35683.0
64	30726.0
65	25769.0
66	22133.5
67	18498.0
68	15506.5
69	12515.0
70	10473.5
71	8432.0
72	7011.5
73	5591.0
74	4684.0
75	2822.0
76	1867.0
77	1571.5
78	1276.0
79	855.5
80	435.0
81	287.5
82	140.0
83	134.5
84	129.0
85	84.5
86	40.0
87	43.5
88	47.0
89	29.0
90	11.0
91	7.0
92	3.0
93	4.5
94	6.0
95	4.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1276954.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.587918669792607
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.99435474227757	22.63333304880569
2	9.669398803253797	5.915335683594342
3	3.574198648768883	3.2798189273467573
4	1.9109503764156468	2.338079787832455
5	1.2410127874803112	1.8979999105810184
6	0.9469922239548342	1.7379912676353897
7	0.7420817295017894	1.588911491183784
8	0.6301886400057874	1.542092709369939
9	0.5281104524102418	1.453841961029267
>10	6.255686474775344	42.90276380823863
>50	0.45887583657408776	8.572292624722417
>100	0.04242342372237173	2.4294606792250053
>500	0.0020821312215695	0.4505930277913168
>1k	0.003383463235050437	2.2699220850554447
>5k	0.0	0.0
>10k+	2.602664026961875E-4	0.9875629875885663
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12405	0.9714523780809645	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTT	3664	0.28693281042230184	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTC	3195	0.2502047841973947	No Hit
CGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG	3014	0.2360304286607035	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTC	2798	0.21911517564454164	No Hit
CCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	2789	0.21841037343553488	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	2273	0.17800171345248145	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCT	2168	0.16977902101406941	No Hit
CTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT	1744	0.13657500583419607	Illumina Single End Adapter 2 (95% over 22bp)
CGTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCT	1657	0.1297619178137975	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCG	1477	0.11566587363366261	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGC	1430	0.11198523987551627	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCC	1301	0.1018830748797529	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3493406966891523E-4	0.0	0.0	0.14440614148982658	0.0
2	2.3493406966891523E-4	0.0	0.0	0.6493577685648817	0.0
3	2.3493406966891523E-4	0.0	0.0	1.099178200624298	0.0
4	2.3493406966891523E-4	0.0	0.0	1.5793051276710046	0.0
5	2.3493406966891523E-4	0.0	0.0	2.793366088363402	0.0
6	2.3493406966891523E-4	0.0	0.0	3.909616164716975	0.0
7	2.3493406966891523E-4	0.0	0.0	4.788191273922162	0.0
8	2.3493406966891523E-4	0.0	0.0	5.979228695787006	0.0
9	2.3493406966891523E-4	0.0	0.0	6.599298016999829	0.0
10	2.3493406966891523E-4	0.0	0.0	7.646164231444516	0.0
11	2.3493406966891523E-4	0.0	0.0	8.712060105532384	0.0
12	2.3493406966891523E-4	0.0	0.0	9.645061607544203	0.0
13	2.3493406966891523E-4	0.0	0.0	10.056352852177918	0.0
14	2.3493406966891523E-4	0.0	0.0	10.299039746145906	0.0
15	2.3493406966891523E-4	0.0	0.0	10.56623809471602	0.0
16	2.3493406966891523E-4	0.0	0.0	11.017546442550005	0.0
17	2.3493406966891523E-4	0.0	0.0	11.557816491431954	0.0
18	2.3493406966891523E-4	0.0	0.0	12.180391776054579	0.0
19	2.3493406966891523E-4	0.0	0.0	12.573436474610675	0.0
20	2.3493406966891523E-4	0.0	0.0	12.977679697154322	0.0
21	2.3493406966891523E-4	0.0	0.0	13.441909418820098	0.0
22	2.3493406966891523E-4	0.0	0.0	13.961740203640852	0.0
23	2.3493406966891523E-4	0.0	0.0	14.47021584176094	0.0
24	2.3493406966891523E-4	0.0	0.0	14.882133577247105	0.0
25	2.3493406966891523E-4	0.0	0.0	15.256461861586244	0.0
26	2.3493406966891523E-4	0.0	0.0	15.621784339921406	0.0
27	2.3493406966891523E-4	0.0	0.0	16.044587353968897	0.0
28	2.3493406966891523E-4	0.0	0.0	16.427294953459562	0.0
29	2.3493406966891523E-4	0.0	0.0	16.846887201888244	0.0
30	2.3493406966891523E-4	0.0	0.0	17.384181419221054	0.0
31	3.915567827815254E-4	0.0	0.0	17.81215298280126	0.0
32	3.915567827815254E-4	0.0	0.0	18.23855831925034	0.0
33	3.915567827815254E-4	0.0	0.0	18.659168615314254	0.0
34	3.915567827815254E-4	0.0	0.0	19.100453109509036	0.0
35	3.915567827815254E-4	0.0	0.0	19.566562303732162	0.0
36	3.915567827815254E-4	0.0	0.0	19.977383680226538	0.0
37	4.6986813933783047E-4	0.0	0.0	20.415144163376283	0.0
38	4.6986813933783047E-4	0.0	0.0	20.936149618545382	0.0
39	4.6986813933783047E-4	0.0	0.0	21.673451040522995	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCG	25	3.891051E-5	44.999996	1
CGTTTTT	7180	0.0	43.401814	1
CGGTCTA	60	3.6379788E-12	41.250004	31
CTCGTAG	50	1.0822987E-9	40.499996	1
TCGATAG	40	3.4586265E-7	39.375	1
TTGCGAG	115	0.0	39.130432	1
CGTTCTG	435	0.0	36.206894	1
GCGCGAC	280	0.0	36.160713	9
ACAACGA	305	0.0	36.14754	13
TTAACGG	75	1.8189894E-12	36.0	2
CGTAAGG	225	0.0	36.0	2
ACTGCGC	25	0.0021071965	35.999996	27
GTCGAGT	25	0.0021071965	35.999996	14
TAGACGG	100	0.0	35.999996	2
CGTCCCG	25	0.0021071965	35.999996	26
GCGTAGT	25	0.0021071965	35.999996	39
GTTTTTT	8955	0.0	35.72864	2
TACGGGA	515	0.0	35.38835	4
TTGTACG	70	2.1827873E-11	35.357143	1
GTATGCG	70	2.1827873E-11	35.357143	1
>>END_MODULE