##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935794.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	722605
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05254599677556	33.0	31.0	34.0	30.0	34.0
2	32.07187052400689	33.0	31.0	34.0	30.0	34.0
3	32.1103687353395	33.0	31.0	34.0	30.0	34.0
4	35.76899966094893	37.0	35.0	37.0	33.0	37.0
5	34.8407165740619	37.0	35.0	37.0	33.0	37.0
6	35.33031739332	37.0	35.0	37.0	33.0	37.0
7	35.90335660561441	37.0	35.0	37.0	35.0	37.0
8	35.89744881366722	37.0	35.0	37.0	35.0	37.0
9	37.741901868932544	39.0	38.0	39.0	35.0	39.0
10	37.39055638972883	39.0	37.0	39.0	34.0	39.0
11	37.32289701842639	39.0	37.0	39.0	34.0	39.0
12	37.1937407020433	39.0	37.0	39.0	34.0	39.0
13	37.06908615356938	39.0	37.0	39.0	33.0	39.0
14	38.08109409705164	40.0	37.0	41.0	33.0	41.0
15	38.231999501802505	40.0	37.0	41.0	33.0	41.0
16	38.28793462541776	40.0	37.0	41.0	34.0	41.0
17	38.28918565467994	40.0	37.0	41.0	34.0	41.0
18	38.27499256163464	40.0	37.0	41.0	34.0	41.0
19	38.22712962130071	40.0	37.0	41.0	34.0	41.0
20	38.22088969769099	40.0	37.0	41.0	34.0	41.0
21	38.12225351333024	40.0	37.0	41.0	34.0	41.0
22	38.16901488365013	40.0	37.0	41.0	34.0	41.0
23	38.1522048698805	40.0	37.0	41.0	34.0	41.0
24	38.11586413047239	40.0	36.0	41.0	34.0	41.0
25	37.96664014226306	40.0	36.0	41.0	34.0	41.0
26	37.98433860822995	40.0	36.0	41.0	34.0	41.0
27	38.00612229364591	40.0	36.0	41.0	34.0	41.0
28	37.90886445568464	40.0	36.0	41.0	34.0	41.0
29	37.822969672227565	40.0	36.0	41.0	34.0	41.0
30	37.730523591727156	40.0	36.0	41.0	33.0	41.0
31	37.67896291888376	40.0	36.0	41.0	33.0	41.0
32	37.59710630289024	40.0	36.0	41.0	33.0	41.0
33	37.50771860144892	40.0	36.0	41.0	33.0	41.0
34	37.42201756146166	40.0	35.0	41.0	33.0	41.0
35	37.36040160253527	40.0	35.0	41.0	33.0	41.0
36	37.305011728399336	40.0	35.0	41.0	33.0	41.0
37	37.269856975802824	40.0	35.0	41.0	33.0	41.0
38	37.18552321115963	39.0	35.0	41.0	33.0	41.0
39	37.118305298191956	39.0	35.0	41.0	32.0	41.0
40	37.02236768358924	39.0	35.0	41.0	32.0	41.0
41	36.94345873610064	39.0	35.0	41.0	32.0	41.0
42	36.97959050933774	39.0	35.0	41.0	32.0	41.0
43	36.884888701296006	39.0	35.0	41.0	32.0	41.0
44	36.702382352737665	39.0	35.0	41.0	31.0	41.0
45	36.67928951501858	39.0	35.0	41.0	31.0	41.0
46	36.579007895046395	39.0	35.0	41.0	31.0	41.0
47	36.500256710097496	39.0	35.0	41.0	31.0	41.0
48	36.48075781374333	39.0	35.0	41.0	31.0	41.0
49	36.48176527978633	39.0	35.0	40.0	31.0	41.0
50	36.36690861535694	38.0	35.0	40.0	31.0	41.0
51	35.188843143902965	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	18.0
10	22.0
11	17.0
12	23.0
13	18.0
14	27.0
15	34.0
16	31.0
17	64.0
18	173.0
19	259.0
20	506.0
21	781.0
22	1249.0
23	1763.0
24	2509.0
25	3477.0
26	4621.0
27	5753.0
28	6573.0
29	7795.0
30	9892.0
31	12653.0
32	16979.0
33	24763.0
34	46230.0
35	61709.0
36	53124.0
37	81518.0
38	141581.0
39	238289.0
40	146.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.87293888085468	20.526982237875465	25.07773956726012	11.522339314009729
2	32.43985303173933	24.114696134125836	24.93962815092616	18.50582268320867
3	32.67455940659143	23.456660277745105	26.806069706132675	17.062710609530797
4	29.348814359158876	24.521834197106305	26.408618816642566	19.720732627092257
5	24.361442281744523	30.866240892327067	24.971042270673465	19.801274555254945
6	25.79652784024467	33.82996242760568	25.286013797302815	15.087495934846839
7	78.52450508922578	6.0875582095335625	9.784598778032258	5.603337923208391
8	78.45918586226223	5.299299063803876	9.87814919631057	6.3633658776233215
9	72.05554902055756	7.516693075746778	12.773368576193079	7.654389327502578
10	40.62689851301887	22.40989198801558	19.939109195203468	17.024100303762083
11	30.13264508272154	24.427453449671674	24.482947114952154	20.95695435265463
12	26.923422893558723	21.51050712353222	31.084479072245557	20.481590910663503
13	24.12272264930356	24.4304979899115	32.47251264522111	18.97426671556383
14	19.790341888030113	28.00243563219186	31.029400571543235	21.17782190823479
15	17.667190235329123	25.935192809349505	35.67038700258094	20.727229952740434
16	21.524069166418723	24.251977221303477	31.499920426789185	22.724033185488615
17	21.765694950906788	24.025158973436387	28.14497547069284	26.064170604963984
18	22.28603455553172	24.63752672621972	31.172078798236935	21.904359920011622
19	23.561420139633686	25.908483888154667	28.070522623009804	22.459573349201847
20	24.289342033337714	26.603331003798758	28.21610700175061	20.891219961112917
21	24.089094318472746	25.6476221448786	29.699628427702546	20.563655108946104
22	22.910026916503483	23.878329100961107	28.598750354619746	24.612893627915668
23	21.055901910449002	24.884549650223843	29.93793289556535	24.121615543761806
24	22.85314936929581	24.9035088326264	28.65078431508224	23.592557482995552
25	21.3312944139606	26.010060821610697	27.542156503207142	25.116488261221555
26	19.861058254509725	27.331391285695506	27.786273275164163	25.02127718463061
27	21.295036707468117	25.98265995945226	29.88077857197224	22.841524761107383
28	19.918489354488276	25.663260010655893	30.230623923166878	24.18762671168896
29	20.109465060441043	25.79680461663011	30.479722670061793	23.61400765286706
30	23.52876052615191	24.68291805343168	30.069678455034214	21.71864296538219
31	24.66229821271649	25.901287702133253	26.137239570719828	23.29917451443043
32	23.725271759813452	25.784903232056237	28.359892333986064	22.129932674144243
33	24.581479508168364	23.795296185329466	28.80411843261533	22.81910587388684
34	21.885815902187225	24.369330408729528	29.603863798340726	24.140989890742524
35	20.70508784190533	24.58618470672082	30.599566844956787	24.10916060641706
36	24.258896630939446	24.38732087378305	31.060953079483262	20.292829415794245
37	21.956393880474117	24.732184250039786	31.349215684917763	21.962206184568334
38	22.65802201756146	25.084105424125212	29.39531279191259	22.862559766400732
39	22.73205970066634	23.42469260522692	29.962012441098523	23.881235253008214
40	23.65483216971928	22.54163754748445	30.269372617128305	23.534157665667966
41	19.668006725666164	24.015748576331468	30.05113443720982	26.26511026079255
42	21.567246282547174	23.25710450384373	31.496875886549358	23.678773327059734
43	23.475065907376784	22.361317732371074	31.197403837504584	22.966212522747558
44	22.83073048207527	22.9700873921437	29.16697227392559	25.03220985185544
45	22.476041544135455	22.517004449180398	28.425349949142337	26.581604057541806
46	24.078161651247914	22.79156662353568	30.222043855218274	22.908227869998132
47	20.370465191909826	23.43202717944105	32.769493706796936	23.428013921852187
48	20.37378650853509	22.54177593567717	31.89349644688315	25.190941108904592
49	22.340005950692287	21.009265089502563	33.53173587229538	23.11899308750977
50	21.449616318735686	20.866725251001583	32.6222486697435	25.061409760519233
51	20.786183322838895	21.520194297022577	28.9166280332962	28.776994346842326
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	452.0
1	713.0
2	974.0
3	1623.0
4	2272.0
5	1691.5
6	1111.0
7	1123.5
8	1136.0
9	1227.0
10	1318.0
11	1212.5
12	1107.0
13	1145.5
14	1184.0
15	1304.0
16	1424.0
17	1463.0
18	1502.0
19	1633.5
20	1765.0
21	1881.5
22	1998.0
23	2213.0
24	2428.0
25	2799.5
26	3894.5
27	4618.0
28	5612.5
29	6607.0
30	7666.0
31	8725.0
32	10492.5
33	12260.0
34	14264.5
35	16269.0
36	17216.5
37	18164.0
38	21245.5
39	24327.0
40	28555.5
41	32784.0
42	40004.0
43	47224.0
44	58411.0
45	69598.0
46	76307.0
47	83016.0
48	83221.0
49	83426.0
50	71601.0
51	59776.0
52	52524.5
53	45273.0
54	40385.5
55	35498.0
56	33419.0
57	31340.0
58	30527.0
59	29714.0
60	26889.5
61	24065.0
62	22329.0
63	20593.0
64	18095.5
65	15598.0
66	13465.0
67	11332.0
68	9385.5
69	7439.0
70	6168.0
71	4897.0
72	4311.0
73	3725.0
74	2959.5
75	1698.5
76	1203.0
77	840.5
78	478.0
79	449.0
80	420.0
81	253.0
82	86.0
83	67.5
84	49.0
85	33.5
86	18.0
87	17.0
88	16.0
89	13.5
90	11.0
91	10.0
92	9.0
93	10.0
94	11.0
95	5.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	722605.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.12683931817282
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.96925914858514	19.680141092838475
2	12.512693896362052	7.038850613209144
3	4.950080886327471	4.176903891050738
4	2.4744041724197774	2.783886742634699
5	1.4643585870551166	2.059388934114292
6	0.9975059970703798	1.6834014539107403
7	0.6721618644900799	1.3234052130821148
8	0.5354033963823239	1.2047364240359693
9	0.40822109806629103	1.0333772308438773
>10	4.988788242186967	35.710704180190454
>50	0.9446169425383559	16.454770398042776
>100	0.07460102422709666	3.9422858461792782
>500	0.003952372144481943	0.7257863579342393
>1k	0.003952372144481943	2.1823616219332242
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	3801	0.5260135205264287	TruSeq Adapter, Index 19 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	3738	0.5172950643851068	TruSeq Adapter, Index 19 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT	2160	0.2989184962738979	TruSeq Adapter, Index 13 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	1446	0.20010932667224834	TruSeq Adapter, Index 19 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGC	1199	0.1659274430705572	No Hit
GAATCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTC	1180	0.16329806740888866	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCG	1139	0.1576241515073934	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1042	0.14420049681361186	No Hit
ACTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	991	0.1371426989849226	TruSeq Adapter, Index 13 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.383881927193972E-4	0.0	0.0	0.312895703738557	0.0
2	1.383881927193972E-4	0.0	0.0	1.7618200815106455	0.0
3	1.383881927193972E-4	0.0	0.0	2.4161194566879556	0.0
4	1.383881927193972E-4	0.0	0.0	3.2094989655482595	0.0
5	1.383881927193972E-4	0.0	0.0	5.215989371786799	0.0
6	1.383881927193972E-4	0.0	0.0	6.23729423405595	0.0
7	1.383881927193972E-4	0.0	0.0	7.223863659952533	0.0
8	1.383881927193972E-4	0.0	0.0	8.726205880114309	0.0
9	1.383881927193972E-4	0.0	0.0	9.253326506182493	0.0
10	2.767763854387944E-4	0.0	0.0	10.59707585748784	0.0
11	2.767763854387944E-4	0.0	0.0	12.475972350039095	0.0
12	2.767763854387944E-4	0.0	0.0	14.030902083434242	0.0
13	2.767763854387944E-4	0.0	0.0	14.60756568249597	0.0
14	2.767763854387944E-4	0.0	0.0	14.86925775492835	0.0
15	2.767763854387944E-4	0.0	0.0	15.310992866088664	0.0
16	2.767763854387944E-4	0.0	0.0	16.292857093432787	0.0
17	2.767763854387944E-4	0.0	0.0	17.407712373980253	0.0
18	2.767763854387944E-4	0.0	0.0	18.690570920489066	0.0
19	2.767763854387944E-4	0.0	0.0	19.447969499242326	0.0
20	2.767763854387944E-4	0.0	0.0	20.127178749109127	0.0
21	2.767763854387944E-4	0.0	0.0	20.98490876758395	0.0
22	2.767763854387944E-4	0.0	0.0	21.838071975699034	0.0
23	2.767763854387944E-4	0.0	0.0	22.671030507677084	0.0
24	2.767763854387944E-4	0.0	0.0	23.35190041585652	0.0
25	2.767763854387944E-4	0.0	0.0	23.948768691055278	0.0
26	2.767763854387944E-4	0.0	0.0	24.490558465551718	0.0
27	2.767763854387944E-4	0.0	0.0	25.05504390365414	0.0
28	2.767763854387944E-4	0.0	0.0	25.6240961521163	0.0
29	2.767763854387944E-4	0.0	0.0	26.221517980085938	0.0
30	2.767763854387944E-4	0.0	0.0	26.903771770192566	0.0
31	2.767763854387944E-4	0.0	0.0	27.4654894444406	0.0
32	4.1516457815819155E-4	0.0	0.0	28.045889524705753	0.0
33	4.1516457815819155E-4	0.0	0.0	28.592107721369214	0.0
34	4.1516457815819155E-4	0.0	0.0	29.157423488627952	0.0
35	4.1516457815819155E-4	0.0	0.0	29.751939164550482	0.0
36	4.1516457815819155E-4	0.0	0.0	30.304523218079034	0.0
37	4.1516457815819155E-4	0.0	0.0	30.859598259076535	0.0
38	4.1516457815819155E-4	0.0	0.0	31.398481881525868	0.0
39	4.1516457815819155E-4	0.0	0.0	32.10952041571813	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGG	20	7.031546E-4	45.0	2
CTTCGAA	20	7.031546E-4	45.0	34
GTACCTA	20	7.031546E-4	45.0	33
TCGTTAG	20	7.031546E-4	45.0	1
GTACCCG	25	3.8893628E-5	45.0	10
ACACGTA	20	7.031546E-4	45.0	42
TTCGTAC	25	3.8893628E-5	45.0	33
TAACGCG	20	7.031546E-4	45.0	1
AGGCGAC	25	3.8893628E-5	45.0	30
TTATCGC	20	7.031546E-4	45.0	35
ACCCTCG	25	3.8893628E-5	45.0	38
TAGTTCG	20	7.031546E-4	45.0	20
CCGTAAC	20	7.031546E-4	45.0	33
AATTCGT	25	3.8893628E-5	45.0	31
ACCGTAA	20	7.031546E-4	45.0	32
TCGACGT	25	3.8893628E-5	45.0	26
TAATGCG	25	3.8893628E-5	45.0	24
TCGGTAA	25	3.8893628E-5	45.0	34
GCGTAAG	20	7.031546E-4	45.0	1
TACGTTC	20	7.031546E-4	45.0	28
>>END_MODULE