SRR2935793.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935793.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	419044
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	3088	0.7369154551789311	RNA PCR Primer, Index 10 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	2501	0.5968346999360449	RNA PCR Primer, Index 35 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT	1675	0.399719361212665	TruSeq Adapter, Index 10 (95% over 24bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGC	993	0.23696795563234407	No Hit
GGAGAAGGGAATGATACCTGTCTCTTATACACATCTGACGCGGTTTGTCTC	963	0.22980880289420683	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCG	935	0.22312692700527867	No Hit
TCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	820	0.19568350817575245	RNA PCR Primer, Index 35 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	817	0.19496759290193869	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTC	799	0.19067210125905631	No Hit
ACTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	594	0.14175122421511824	RNA PCR Primer, Index 35 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG	590	0.14079667051669992	TruSeq Adapter, Index 10 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCC	489	0.11669418963163773	No Hit
GGAGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTC	473	0.11287597483796452	No Hit
GGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG	451	0.10762592949666383	RNA PCR Primer, Index 10 (95% over 22bp)
GGAGAAGGGAATGATACGGCTGTCTCTTATACACATCTGACGCGGTTTGTC	446	0.10643273737364095	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACAT	20	7.028149E-4	45.000004	10
ACGCACG	20	7.028149E-4	45.000004	1
CGATTAG	30	2.1621872E-6	45.000004	10
TAGTTCG	30	2.1621872E-6	45.000004	1
CGTTAGC	20	7.028149E-4	45.000004	40
TGGCACG	20	7.028149E-4	45.000004	10
ACTAGCG	20	7.028149E-4	45.000004	1
TCCGTTA	20	7.028149E-4	45.000004	38
ACGTATG	40	6.7975634E-9	45.000004	1
CGCGTAG	20	7.028149E-4	45.000004	10
ATACGAC	20	7.028149E-4	45.000004	13
TAAGCGC	20	7.028149E-4	45.000004	41
TAGTACG	20	7.028149E-4	45.000004	1
TGGTAAG	20	7.028149E-4	45.000004	1
TCGTTAG	25	3.8865473E-5	45.0	23
ACTGCGC	25	3.8865473E-5	45.0	27
GTCTACG	25	3.8865473E-5	45.0	1
GCGTTAG	25	3.8865473E-5	45.0	1
ACGGGTA	45	3.8380676E-10	45.0	5
AGGTTCG	25	3.8865473E-5	45.0	1