##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935793.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	419044
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.993859833334923	33.0	31.0	34.0	30.0	34.0
2	32.05229761075209	33.0	31.0	34.0	30.0	34.0
3	32.04041103082254	33.0	31.0	34.0	30.0	34.0
4	35.724360687660486	37.0	35.0	37.0	33.0	37.0
5	34.92271455980756	37.0	35.0	37.0	33.0	37.0
6	35.35044052653182	37.0	35.0	37.0	33.0	37.0
7	35.899459245329844	37.0	35.0	37.0	35.0	37.0
8	35.878912476971394	37.0	35.0	37.0	35.0	37.0
9	37.66229083342083	39.0	38.0	39.0	35.0	39.0
10	37.30878857590134	39.0	37.0	39.0	34.0	39.0
11	37.22541785588148	39.0	37.0	39.0	34.0	39.0
12	37.05415183131127	39.0	37.0	39.0	34.0	39.0
13	36.925148671738526	39.0	37.0	39.0	33.0	39.0
14	37.93592081022518	40.0	37.0	41.0	33.0	41.0
15	38.09902301428967	40.0	37.0	41.0	33.0	41.0
16	38.12187025706131	40.0	37.0	41.0	34.0	41.0
17	38.138536764635695	40.0	37.0	41.0	34.0	41.0
18	38.15624134935711	40.0	37.0	41.0	34.0	41.0
19	38.0850936894455	40.0	36.0	41.0	34.0	41.0
20	38.0481572340852	40.0	36.0	41.0	34.0	41.0
21	37.94736113630072	40.0	36.0	41.0	34.0	41.0
22	37.98783421311366	40.0	36.0	41.0	34.0	41.0
23	37.97970141560314	40.0	36.0	41.0	34.0	41.0
24	37.993299033037104	40.0	36.0	41.0	34.0	41.0
25	37.83615801681923	40.0	36.0	41.0	34.0	41.0
26	37.88875869837058	40.0	36.0	41.0	34.0	41.0
27	37.86238676606752	40.0	36.0	41.0	34.0	41.0
28	37.659033418924984	40.0	36.0	41.0	33.0	41.0
29	37.65019902444612	40.0	36.0	41.0	33.0	41.0
30	37.55022384284228	40.0	36.0	41.0	33.0	41.0
31	37.39506113916438	39.0	35.0	41.0	33.0	41.0
32	37.367980450740255	39.0	35.0	41.0	33.0	41.0
33	37.281359475377286	39.0	35.0	41.0	33.0	41.0
34	37.19189870276153	39.0	35.0	41.0	32.0	41.0
35	37.15990444917479	39.0	35.0	41.0	32.0	41.0
36	37.04228195607144	39.0	35.0	41.0	32.0	41.0
37	36.92541594677409	39.0	35.0	41.0	31.0	41.0
38	36.8235674535371	39.0	35.0	41.0	31.0	41.0
39	36.84860778343086	39.0	35.0	41.0	31.0	41.0
40	36.76192714846174	39.0	35.0	41.0	31.0	41.0
41	36.664832332642874	39.0	35.0	41.0	31.0	41.0
42	36.67152614045303	39.0	35.0	41.0	31.0	41.0
43	36.51561649850613	39.0	35.0	41.0	31.0	41.0
44	36.299133265241835	39.0	35.0	41.0	30.0	41.0
45	36.29833382651941	39.0	35.0	40.0	30.0	41.0
46	36.11073061540077	38.0	35.0	40.0	30.0	41.0
47	35.993425511402144	38.0	35.0	40.0	30.0	41.0
48	35.963528889567684	38.0	35.0	40.0	29.0	41.0
49	35.9448673647636	38.0	35.0	40.0	29.0	41.0
50	35.78949227288781	38.0	35.0	40.0	29.0	41.0
51	34.412085604375676	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	6.0
10	10.0
11	13.0
12	6.0
13	11.0
14	17.0
15	16.0
16	27.0
17	43.0
18	91.0
19	162.0
20	376.0
21	640.0
22	834.0
23	1224.0
24	1757.0
25	2312.0
26	3061.0
27	3622.0
28	4264.0
29	5272.0
30	6481.0
31	8108.0
32	10658.0
33	15548.0
34	28625.0
35	39571.0
36	31222.0
37	46681.0
38	82333.0
39	125982.0
40	65.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.51258579051365	21.10351180305648	24.672110804593313	12.71179160183656
2	32.872681627704964	23.8318649115606	25.292332070140606	18.00312139059383
3	30.566479892326342	24.96396559788471	27.469430417808155	17.000124091980794
4	31.147087179389278	25.151058122774693	25.87723484884642	17.824619848989606
5	25.13673981729842	30.602037017592426	24.829134887983123	19.43208827712603
6	25.563902597340615	34.204045398573896	24.63822414829946	15.593827855786028
7	78.60009927358463	6.095064002825479	9.98964309237216	5.315193631217724
8	78.7339754297878	5.662889815866592	9.130544763795688	6.472589990549918
9	72.67685493647446	7.2968471091341245	12.00876280295148	8.017535151439944
10	41.29542482412349	24.262845906396464	19.75448878876681	14.687240480713243
11	30.64021916552916	26.589809184715683	23.79917144738977	18.970800202365385
12	27.045131298861218	21.52637909145579	30.70966294708909	20.718826662593905
13	24.722940789034087	25.81972298851672	30.263170454653928	19.19416576779527
14	19.753295596643788	30.724458529414573	28.862362902225065	20.659882971716574
15	18.48350053932284	24.60576932255324	37.15361632668645	19.757113811437463
16	19.813671118068747	26.31155678162675	31.72864902015063	22.146123080153874
17	19.318973663863463	24.62557631179542	28.446654766563896	27.608795257777224
18	22.558967554719793	24.668769866648848	31.147325817813883	21.62493676081748
19	23.15293859356058	26.13472570899476	28.06220826452592	22.65012743291874
20	24.129447027042506	25.878905317818653	28.904363264955467	21.08728439018337
21	23.092085795286412	26.395557507087563	30.172487853304187	20.33986884432184
22	20.58137093002167	25.21692232796556	27.743625967678813	26.458080774333958
23	18.940015845591393	26.416796326877368	29.310287225207855	25.332900602323384
24	22.08884985824878	23.57485132826147	29.197649888794498	25.138648924695257
25	20.121753324233254	26.745640075982475	28.633747291453883	24.49885930833039
26	20.35036893500444	27.445327936923093	28.184868414772673	24.0194347132998
27	22.773980775288514	26.960891934975802	28.041446721585324	22.22368056815036
28	18.641717814835673	25.03484120999227	33.35974265232291	22.96369832284915
29	21.776949437290593	24.127299281221067	31.525567720812138	22.570183560676206
30	23.697750116932827	24.213925029352527	30.657639770525293	21.430685083189356
31	25.178978818453434	25.28326381000563	28.379358730825405	21.158398640715532
32	24.965874705281546	25.210479090501238	28.388904267809583	21.434741936407633
33	23.25793950038659	24.67282671986713	28.628974522961787	23.44025925678449
34	22.34657935682172	23.016437414686763	31.405293954811427	23.23168927368009
35	22.482603258846325	23.068221952825958	30.339773388951997	24.10940139937572
36	25.405685321827782	22.980164374146867	30.079180229283796	21.534970074741555
37	22.80046964041962	23.037914872901176	32.77078302039882	21.39083246628039
38	19.630635446397037	26.422285010643275	30.843300464867653	23.10377907809204
39	21.957360086291654	22.99114174167868	31.68975095694008	23.361747215089583
40	25.25224081480704	22.2153282232892	30.891744065062383	21.640686896841384
41	22.399079810234728	22.256851309170397	28.051469535418715	27.29259934517616
42	23.713977529805938	21.442139727570375	29.48330008304617	25.360582659577513
43	24.63178091083514	21.478651406534873	29.38832199005355	24.501245692576436
44	21.68292589799639	22.820515268086407	31.110575500424776	24.385983333492426
45	22.09290671146705	22.742719141665315	29.528164106871834	25.6362100399958
46	24.601951107759568	22.71002567749449	30.950926394364313	21.73709682038163
47	21.42567367627266	22.645593302851253	32.58822462557631	23.34050839529978
48	20.858668779412184	21.541174673781274	32.387768348908466	25.212388197898072
49	22.257805862868814	19.42469048596329	33.873531180496556	24.443972470671337
50	21.937075820200267	20.669428508700758	32.320710951594585	25.072784719504398
51	20.489972413398117	21.139307566747167	29.16089957140539	29.209820448449324
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	426.0
1	532.5
2	639.0
3	1087.5
4	1536.0
5	1087.0
6	638.0
7	665.0
8	692.0
9	728.0
10	764.0
11	837.0
12	910.0
13	828.5
14	747.0
15	795.0
16	843.0
17	845.0
18	847.0
19	827.5
20	808.0
21	949.0
22	1090.0
23	1146.0
24	1202.0
25	1366.0
26	1746.5
27	1963.0
28	2638.0
29	3313.0
30	4138.0
31	4963.0
32	5179.0
33	5395.0
34	6632.0
35	7869.0
36	9017.0
37	10165.0
38	12075.5
39	13986.0
40	15685.0
41	17384.0
42	21795.0
43	26206.0
44	29141.0
45	32076.0
46	39089.0
47	46102.0
48	49205.5
49	52309.0
50	51953.5
51	51598.0
52	42748.5
53	33899.0
54	29350.5
55	24802.0
56	22098.5
57	19395.0
58	17431.5
59	15468.0
60	13287.0
61	11106.0
62	10226.5
63	9347.0
64	8125.0
65	6903.0
66	5849.0
67	4795.0
68	3842.5
69	2890.0
70	2458.5
71	2027.0
72	1537.5
73	1048.0
74	837.5
75	455.5
76	284.0
77	281.5
78	279.0
79	191.5
80	104.0
81	74.5
82	45.0
83	29.5
84	14.0
85	9.0
86	4.0
87	2.5
88	1.0
89	1.0
90	1.0
91	1.5
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	419044.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.037527065492
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.91728456935365	23.04051950867358
2	11.990446503270476	7.682885087517237
3	4.69873432590484	4.516074844191979
4	2.259658331728697	2.895754598460905
5	1.2972237848735442	2.077992105894307
6	0.8151859753903503	1.5669925649986705
7	0.6168038988799373	1.3832610123126887
8	0.4622776053558261	1.184818503468649
9	0.3444369804044508	0.9931418173857539
>10	5.158849996887318	40.88567228327281
>50	0.37498524288507623	7.201711268837764
>100	0.05591231255741694	3.281169681666607
>500	0.005963980006124473	1.5550804918715446
>1k	0.002236492502296677	1.7349262314475349
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	3088	0.7369154551789311	RNA PCR Primer, Index 10 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	2501	0.5968346999360449	RNA PCR Primer, Index 35 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT	1675	0.399719361212665	TruSeq Adapter, Index 10 (95% over 24bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGC	993	0.23696795563234407	No Hit
GGAGAAGGGAATGATACCTGTCTCTTATACACATCTGACGCGGTTTGTCTC	963	0.22980880289420683	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCG	935	0.22312692700527867	No Hit
TCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	820	0.19568350817575245	RNA PCR Primer, Index 35 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	817	0.19496759290193869	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTC	799	0.19067210125905631	No Hit
ACTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	594	0.14175122421511824	RNA PCR Primer, Index 35 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG	590	0.14079667051669992	TruSeq Adapter, Index 10 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCC	489	0.11669418963163773	No Hit
GGAGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTC	473	0.11287597483796452	No Hit
GGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG	451	0.10762592949666383	RNA PCR Primer, Index 10 (95% over 22bp)
GGAGAAGGGAATGATACGGCTGTCTCTTATACACATCTGACGCGGTTTGTC	446	0.10643273737364095	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4369469554509789	0.0
2	0.0	0.0	0.0	2.2670650337434735	0.0
3	0.0	0.0	0.0	3.0230715628907703	0.0
4	0.0	0.0	0.0	3.817021601550195	0.0
5	0.0	0.0	0.0	5.953312778610361	0.0
6	0.0	0.0	0.0	6.912161968671548	0.0
7	0.0	0.0	0.0	7.944511793510944	0.0
8	0.0	0.0	0.0	9.594696499651588	0.0
9	0.0	0.0	0.0	10.15597407432155	0.0
10	0.0	0.0	0.0	11.709748856921946	0.0
11	0.0	0.0	0.0	13.709538855108294	0.0
12	0.0	0.0	0.0	15.478088219852808	0.0
13	0.0	0.0	0.0	16.224310573591318	0.0
14	0.0	0.0	0.0	16.494210631819094	0.0
15	0.0	0.0	0.0	17.016828781703115	0.0
16	0.0	0.0	0.0	18.16014547398364	0.0
17	0.0	0.0	0.0	19.439724706713378	0.0
18	0.0	0.0	0.0	21.177966991533108	0.0
19	0.0	0.0	0.0	21.980269375053695	0.0
20	0.0	0.0	0.0	22.88948177279713	0.0
21	0.0	0.0	0.0	23.82828533519153	0.0
22	0.0	0.0	0.0	24.828180334284706	0.0
23	0.0	0.0	0.0	25.77605215681408	0.0
24	0.0	0.0	0.0	26.48838785425874	0.0
25	0.0	0.0	0.0	27.115290995694963	0.0
26	0.0	0.0	0.0	27.704727904468267	0.0
27	0.0	0.0	0.0	28.361222210555454	0.0
28	0.0	0.0	0.0	29.04515993547217	0.0
29	0.0	0.0	0.0	29.67564265327746	0.0
30	0.0	0.0	0.0	30.402535294623	0.0
31	2.3863842460457614E-4	0.0	0.0	31.065234199749906	0.0
32	2.3863842460457614E-4	0.0	0.0	31.653716554824793	0.0
33	2.3863842460457614E-4	0.0	0.0	32.238380695106	0.0
34	2.3863842460457614E-4	0.0	0.0	32.819942535867355	0.0
35	2.3863842460457614E-4	0.0	0.0	33.44517520833134	0.0
36	2.3863842460457614E-4	0.0	0.0	33.97495251095351	0.0
37	2.3863842460457614E-4	0.0	0.0	34.523343610694816	0.0
38	2.3863842460457614E-4	0.0	0.0	35.119462395357054	0.0
39	2.3863842460457614E-4	0.0	0.0	35.72655854755109	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACAT	20	7.028149E-4	45.000004	10
ACGCACG	20	7.028149E-4	45.000004	1
CGATTAG	30	2.1621872E-6	45.000004	10
TAGTTCG	30	2.1621872E-6	45.000004	1
CGTTAGC	20	7.028149E-4	45.000004	40
TGGCACG	20	7.028149E-4	45.000004	10
ACTAGCG	20	7.028149E-4	45.000004	1
TCCGTTA	20	7.028149E-4	45.000004	38
ACGTATG	40	6.7975634E-9	45.000004	1
CGCGTAG	20	7.028149E-4	45.000004	10
ATACGAC	20	7.028149E-4	45.000004	13
TAAGCGC	20	7.028149E-4	45.000004	41
TAGTACG	20	7.028149E-4	45.000004	1
TGGTAAG	20	7.028149E-4	45.000004	1
TCGTTAG	25	3.8865473E-5	45.0	23
ACTGCGC	25	3.8865473E-5	45.0	27
GTCTACG	25	3.8865473E-5	45.0	1
GCGTTAG	25	3.8865473E-5	45.0	1
ACGGGTA	45	3.8380676E-10	45.0	5
AGGTTCG	25	3.8865473E-5	45.0	1
>>END_MODULE