##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935792.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1169438
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14161332195465	33.0	31.0	34.0	30.0	34.0
2	32.15977332701691	33.0	31.0	34.0	30.0	34.0
3	32.18764055896935	34.0	31.0	34.0	30.0	34.0
4	35.83782209916216	37.0	35.0	37.0	35.0	37.0
5	34.89295627472342	37.0	35.0	37.0	33.0	37.0
6	35.376816898373406	37.0	35.0	37.0	33.0	37.0
7	35.953838510464	37.0	35.0	37.0	35.0	37.0
8	35.96265043550834	37.0	35.0	37.0	35.0	37.0
9	37.825760750035485	39.0	38.0	39.0	35.0	39.0
10	37.43474557864547	39.0	37.0	39.0	35.0	39.0
11	37.338745619690826	39.0	37.0	39.0	34.0	39.0
12	37.18889244235265	39.0	37.0	39.0	34.0	39.0
13	37.09432907088704	39.0	37.0	39.0	33.0	39.0
14	38.06643276514018	40.0	37.0	41.0	33.0	41.0
15	38.23138122756401	40.0	37.0	41.0	33.0	41.0
16	38.30022797275272	40.0	37.0	41.0	34.0	41.0
17	38.30889880438296	40.0	37.0	41.0	34.0	41.0
18	38.28943218879496	40.0	37.0	41.0	34.0	41.0
19	38.1943805486054	40.0	37.0	41.0	34.0	41.0
20	38.18356937263882	40.0	37.0	41.0	34.0	41.0
21	38.08450896926558	40.0	36.0	41.0	34.0	41.0
22	38.16090378455292	40.0	37.0	41.0	34.0	41.0
23	38.15613226182149	40.0	36.0	41.0	34.0	41.0
24	38.11061980199036	40.0	36.0	41.0	34.0	41.0
25	37.94970233565183	40.0	36.0	41.0	34.0	41.0
26	37.96483610075951	40.0	36.0	41.0	34.0	41.0
27	37.9730237943354	40.0	36.0	41.0	34.0	41.0
28	37.880218532320654	40.0	36.0	41.0	34.0	41.0
29	37.819138765800325	40.0	36.0	41.0	34.0	41.0
30	37.68380281810579	40.0	36.0	41.0	33.0	41.0
31	37.62153615668381	40.0	35.0	41.0	33.0	41.0
32	37.555621589173604	40.0	35.0	41.0	33.0	41.0
33	37.45012732611733	40.0	35.0	41.0	33.0	41.0
34	37.347139395162465	40.0	35.0	41.0	33.0	41.0
35	37.253482441993505	40.0	35.0	41.0	32.0	41.0
36	37.17648990369733	40.0	35.0	41.0	32.0	41.0
37	37.12829239344027	40.0	35.0	41.0	32.0	41.0
38	37.04165590651236	39.0	35.0	41.0	32.0	41.0
39	36.976606711941976	39.0	35.0	41.0	32.0	41.0
40	36.82685956844228	39.0	35.0	41.0	31.0	41.0
41	36.69413513157602	39.0	35.0	41.0	31.0	41.0
42	36.71740528356356	39.0	35.0	41.0	31.0	41.0
43	36.64782656284472	39.0	35.0	41.0	31.0	41.0
44	36.50395061559484	39.0	35.0	41.0	31.0	41.0
45	36.47592604310788	39.0	35.0	41.0	31.0	41.0
46	36.37560007456573	39.0	35.0	41.0	31.0	41.0
47	36.285127556997466	38.0	35.0	41.0	31.0	41.0
48	36.211162968879066	38.0	35.0	41.0	31.0	41.0
49	36.20005506918708	38.0	35.0	40.0	31.0	41.0
50	36.087177772571096	38.0	35.0	40.0	30.0	41.0
51	34.89812114879113	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	15.0
9	27.0
10	46.0
11	33.0
12	25.0
13	31.0
14	41.0
15	58.0
16	92.0
17	189.0
18	335.0
19	541.0
20	851.0
21	1454.0
22	2186.0
23	3130.0
24	4624.0
25	6648.0
26	8623.0
27	10224.0
28	11583.0
29	13642.0
30	16696.0
31	21416.0
32	29042.0
33	42286.0
34	78619.0
35	88872.0
36	85021.0
37	127561.0
38	222611.0
39	392608.0
40	307.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.714909212801366	21.790723407311887	24.38880898346043	12.105558396426318
2	33.19585989167446	23.943637884180262	25.2812889610223	17.57921326312297
3	32.93949743381009	23.75517128740472	26.420297613041477	16.885033665743716
4	29.117063067900993	25.092736853086695	26.326662892774138	19.463537186238174
5	24.64380326276382	30.85430779571042	25.153877332530666	19.34801160899509
6	26.480668492044895	34.12297188906124	24.52152230387588	14.874837315017983
7	80.35714591111287	5.877695097987239	8.963707353446699	4.801451637453204
8	81.79159562114452	4.975381337018294	8.456369640801821	4.776653401035369
9	76.21601145165455	6.521166577450023	10.514281218841871	6.74854075205355
10	42.467749465982806	23.8305066194189	18.288870380473355	15.412873534124937
11	31.475717395877332	25.587931981002843	23.70266743512696	19.233683187992863
12	28.667530899457688	22.373310940810885	28.850695804309424	20.108462355422006
13	26.25782640892463	23.27964372630272	30.77974206413679	19.68278780063586
14	21.780889623904816	25.428111622847897	30.989244406287465	21.80175434695982
15	20.200728897128364	25.117535089504532	33.78716956349973	20.894566449867373
16	25.33242463473908	24.223686933381675	29.37359654808549	21.070291883793754
17	25.39005915662053	23.87445935569051	27.606593936574665	23.128887551114296
18	26.322045290130813	24.39727458830652	29.061138769220772	20.219541352341892
19	25.355341625635564	26.530692520680876	26.556003824059076	21.557962029624488
20	25.435722116093373	27.14560327268312	27.071636119229918	20.34703849199359
21	24.79353330403151	26.528469230519274	29.161015804172603	19.516981661276613
22	24.13176243631556	23.304099918080308	29.25422296863964	23.309914676964492
23	22.515858044633404	25.552359338417258	30.044773643408202	21.887008973541136
24	23.340014605306138	25.35465753635507	28.817260940725376	22.488066917613416
25	24.732734869227784	25.925615552085702	26.492469032133382	22.84918054655313
26	22.396655487507676	25.597081675129424	27.24607888575538	24.760183951607527
27	22.16868273478372	24.807642645441657	29.45739748494576	23.566277134828866
28	20.738423071595076	25.267179619612158	29.3662425883202	24.62815472047257
29	22.03425919116704	27.10481444933378	27.670556284300662	23.190370075198512
30	24.049928256136706	24.874426861449688	29.371031213283644	21.70461366912996
31	25.117706111824656	25.200822959404434	27.3179937713671	22.363477157403814
32	25.092736853086695	25.85823275795724	26.721211385297895	22.327819003658167
33	25.05254660785779	24.282347589183864	27.851668921310917	22.813436881647423
34	23.41158744627761	24.503907004903212	29.569160571146142	22.515344977673035
35	22.575715856676453	25.89226619966172	29.19718702487862	22.33483091878321
36	25.736464866029667	26.164106177497224	27.370070067844555	20.729358888628553
37	23.759275823087673	26.32546573653328	27.746917750235582	22.16834069014347
38	24.102175574934286	25.674811319625324	27.756751533642653	22.466261571797734
39	24.195040694761072	24.143905021044297	27.602745934371896	24.058308349822735
40	25.27154068877529	23.142398314404016	29.842368727542635	21.743692269278064
41	23.07664023231672	24.06942480063073	28.442037970375512	24.411896996677036
42	23.05902493334405	24.63619276951835	29.404637099187813	22.900145197949783
43	23.247320507799472	23.55498966170075	29.364703387439096	23.832986443060683
44	23.45254729194707	23.673935685346294	28.699939629120998	24.17357739358564
45	22.903993200152552	23.47178730296091	27.63635182027606	25.987867676610477
46	23.25963411484833	24.005034897104423	28.936463497851108	23.798867490196145
47	22.3708311171691	24.596515591249815	30.166541535335778	22.866111756245306
48	21.751559296003723	25.742792691874218	29.111590353657057	23.394057658465005
49	23.795960110754056	23.685821736594843	29.781228248098657	22.736989904552445
50	22.03203590100544	23.73866763351285	29.481597143243164	24.747699322238546
51	21.39737207102899	23.94321032837996	28.61613869226073	26.043278908330326
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	424.0
1	994.0
2	1564.0
3	4239.5
4	6915.0
5	4609.0
6	2303.0
7	2275.5
8	2248.0
9	2280.0
10	2312.0
11	2390.0
12	2468.0
13	2458.5
14	2449.0
15	2382.0
16	2315.0
17	2280.5
18	2246.0
19	2538.5
20	2831.0
21	3059.0
22	3287.0
23	4021.5
24	4756.0
25	5296.0
26	6913.5
27	7991.0
28	9395.0
29	10799.0
30	13693.5
31	16588.0
32	20456.5
33	24325.0
34	27119.0
35	29913.0
36	32350.0
37	34787.0
38	37179.0
39	39571.0
40	43113.0
41	46655.0
42	53388.0
43	60121.0
44	64843.5
45	69566.0
46	81443.5
47	93321.0
48	102312.5
49	111304.0
50	111099.5
51	110895.0
52	99547.5
53	88200.0
54	80961.5
55	73723.0
56	70098.5
57	66474.0
58	62316.0
59	58158.0
60	54086.0
61	50014.0
62	44696.5
63	39379.0
64	35201.5
65	31024.0
66	26370.0
67	21716.0
68	18692.5
69	15669.0
70	13527.5
71	11386.0
72	9091.5
73	6797.0
74	5515.5
75	3395.5
76	2557.0
77	1927.0
78	1297.0
79	890.0
80	483.0
81	375.0
82	267.0
83	214.0
84	161.0
85	108.5
86	56.0
87	38.5
88	21.0
89	16.0
90	11.0
91	12.0
92	13.0
93	8.0
94	3.0
95	3.5
96	4.0
97	2.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1169438.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.64504417326304
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.90050139228593	20.882380826350545
2	10.565187370920174	6.052805178776184
3	3.770957626864542	3.240577433911139
4	1.8321024687747698	2.0992262459199025
5	1.1438004543674547	1.6382107270377038
6	0.8149169030863481	1.4005998411868295
7	0.6158856606804192	1.234945036910893
8	0.5056336153328316	1.1587117797356523
9	0.43427663378518	1.1195886022373214
>10	6.890978970239674	48.61362188642661
>50	0.4716590898633156	8.103755172245974
>100	0.04842082782073622	2.504678275118487
>500	0.003287833987608655	0.6496717970488808
>1k	0.0023911519909881125	1.3012271970939497
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4070	0.3480304214503035	No Hit
GCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC	2228	0.19051886461702117	TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC	2063	0.17640952320687373	TruSeq Adapter, Index 14 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTC	1534	0.1311741195343404	No Hit
CTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGCT	1524	0.13031900793372542	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGC	1446	0.12364913744892846	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCG	1270	0.10859917327810452	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.5653348018449886E-4	0.0	0.0	0.1377584788590759	0.0
2	2.5653348018449886E-4	0.0	0.0	0.6519370843088732	0.0
3	2.5653348018449886E-4	0.0	0.0	0.9635397515729778	0.0
4	2.5653348018449886E-4	0.0	0.0	1.3716845185465154	0.0
5	2.5653348018449886E-4	0.0	0.0	2.4264646779051136	0.0
6	2.5653348018449886E-4	0.0	0.0	3.2060699241858055	0.0
7	2.5653348018449886E-4	0.0	0.0	3.8606578544565853	0.0
8	2.5653348018449886E-4	0.0	0.0	4.8902977327571024	0.0
9	2.5653348018449886E-4	0.0	0.0	5.3613787135359035	0.0
10	2.5653348018449886E-4	0.0	0.0	6.341507630160812	0.0
11	2.5653348018449886E-4	0.0	0.0	7.485988996423924	0.0
12	2.5653348018449886E-4	0.0	0.0	8.445253189993826	0.0
13	2.5653348018449886E-4	0.0	0.0	8.837065325395617	0.0
14	2.5653348018449886E-4	0.0	0.0	9.038102062700203	0.0
15	2.5653348018449886E-4	0.0	0.0	9.327984895308687	0.0
16	2.5653348018449886E-4	0.0	0.0	9.846268036441437	0.0
17	2.5653348018449886E-4	0.0	0.0	10.434755839984676	0.0
18	2.5653348018449886E-4	0.0	0.0	11.119614720917227	0.0
19	2.5653348018449886E-4	0.0	0.0	11.539731050299375	0.0
20	3.4204464024599853E-4	0.0	0.0	11.985158683059726	0.0
21	4.2755580030749815E-4	0.0	0.0	12.513190096439486	0.0
22	4.2755580030749815E-4	0.0	0.0	13.084319134490242	0.0
23	4.2755580030749815E-4	0.0	0.0	13.631505047723778	0.0
24	4.2755580030749815E-4	0.0	0.0	14.087279530851571	0.0
25	4.2755580030749815E-4	0.0	0.0	14.493543052303757	0.0
26	5.985781204304974E-4	0.0	0.0	14.892538125150713	0.0
27	5.985781204304974E-4	0.0	0.0	15.352759188601704	0.0
28	5.985781204304974E-4	0.0	0.0	15.76936956042133	0.0
29	5.985781204304974E-4	0.0	0.0	16.207870789216702	0.0
30	5.985781204304974E-4	0.0	0.0	16.74778825384501	0.0
31	6.840892804919971E-4	0.0	0.0	17.18526334871964	0.0
32	7.696004405534966E-4	0.0	0.0	17.612049548586587	0.0
33	7.696004405534966E-4	0.0	0.0	18.06166722818995	0.0
34	7.696004405534966E-4	0.0	0.0	18.50136561322618	0.0
35	7.696004405534966E-4	0.0	0.0	18.993567850540174	0.0
36	7.696004405534966E-4	0.0	0.0	19.425313697690687	0.0
37	8.551116006149963E-4	0.0	0.0	19.865696172007407	0.0
38	8.551116006149963E-4	0.0	0.0	20.395181275108214	0.0
39	8.551116006149963E-4	0.0	0.0	21.230625308909065	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATCGT	20	7.0333364E-4	45.000004	18
CCCGATA	20	7.0333364E-4	45.000004	30
GTTAACG	60	3.6379788E-12	41.249996	1
TAGTACG	55	6.184564E-11	40.909092	1
ATATGCG	50	1.0822987E-9	40.500004	1
TTAAGCG	45	1.9283107E-8	40.0	1
TACGGGA	360	0.0	39.375	4
CGTTTTT	2060	0.0	39.32039	1
CGCATCG	35	6.248383E-6	38.571426	21
ACGGTTG	35	6.248383E-6	38.571426	1
CGTATGG	120	0.0	37.499996	2
GCTATCG	30	1.139934E-4	37.499996	16
TCAATCG	30	1.139934E-4	37.499996	30
GCGATGT	200	0.0	37.125004	9
ACGGTCT	140	0.0	36.964283	30
TAACGCC	55	2.750312E-9	36.81818	12
ACGGGAA	400	0.0	36.562504	5
GATCGCG	25	0.0021071231	36.000004	1
CGTTCGA	25	0.0021071231	36.000004	14
TAGTTCG	25	0.0021071231	36.000004	32
>>END_MODULE