##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935791.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	507318
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.96757852077001	33.0	31.0	34.0	30.0	34.0
2	31.994465010112002	33.0	31.0	34.0	30.0	34.0
3	32.02098289435818	33.0	31.0	34.0	30.0	34.0
4	35.71006942391163	37.0	35.0	37.0	33.0	37.0
5	34.7321384220548	37.0	35.0	37.0	33.0	37.0
6	35.239303553195434	37.0	35.0	37.0	33.0	37.0
7	35.837656065820646	37.0	35.0	37.0	35.0	37.0
8	35.82932204258473	37.0	35.0	37.0	35.0	37.0
9	37.66316590383152	39.0	37.0	39.0	35.0	39.0
10	37.29545176792465	39.0	37.0	39.0	34.0	39.0
11	37.22232997843562	39.0	37.0	39.0	34.0	39.0
12	37.101890727315016	39.0	37.0	39.0	34.0	39.0
13	36.96554429371716	39.0	37.0	39.0	33.0	39.0
14	37.95134412735207	40.0	37.0	41.0	33.0	41.0
15	38.08767479174798	40.0	37.0	41.0	33.0	41.0
16	38.12736587308158	40.0	37.0	41.0	33.0	41.0
17	38.13613749167189	40.0	37.0	41.0	34.0	41.0
18	38.12059497198995	40.0	37.0	41.0	34.0	41.0
19	38.04273256616166	40.0	36.0	41.0	34.0	41.0
20	38.06703290638219	40.0	36.0	41.0	34.0	41.0
21	37.93408079350624	40.0	36.0	41.0	34.0	41.0
22	37.98723285986304	40.0	36.0	41.0	34.0	41.0
23	38.00252898576435	40.0	36.0	41.0	34.0	41.0
24	37.94019135926579	40.0	36.0	41.0	34.0	41.0
25	37.781369870574274	40.0	36.0	41.0	33.0	41.0
26	37.82375748544305	40.0	36.0	41.0	34.0	41.0
27	37.81606605718701	40.0	36.0	41.0	34.0	41.0
28	37.714478887009726	40.0	36.0	41.0	33.0	41.0
29	37.69080734371735	40.0	36.0	41.0	33.0	41.0
30	37.57600361114725	40.0	36.0	41.0	33.0	41.0
31	37.52469259911929	40.0	36.0	41.0	33.0	41.0
32	37.44698591416035	40.0	35.0	41.0	33.0	41.0
33	37.30961251128483	40.0	35.0	41.0	33.0	41.0
34	37.2139348495421	40.0	35.0	41.0	32.0	41.0
35	37.184253269152684	40.0	35.0	41.0	32.0	41.0
36	37.11963896412112	39.0	35.0	41.0	32.0	41.0
37	37.067886414438284	39.0	35.0	41.0	32.0	41.0
38	36.96435569011941	39.0	35.0	41.0	32.0	41.0
39	36.89980446189569	39.0	35.0	41.0	31.0	41.0
40	36.76824595224297	39.0	35.0	41.0	31.0	41.0
41	36.702884581268556	39.0	35.0	41.0	31.0	41.0
42	36.7173725355694	39.0	35.0	41.0	31.0	41.0
43	36.58518720013877	39.0	35.0	41.0	31.0	41.0
44	36.4584126721307	39.0	35.0	41.0	31.0	41.0
45	36.37436479683355	39.0	35.0	41.0	31.0	41.0
46	36.24803969108133	38.0	35.0	40.0	30.0	41.0
47	36.13116033730323	38.0	35.0	40.0	30.0	41.0
48	36.09684655383803	38.0	35.0	40.0	30.0	41.0
49	36.084536720557914	38.0	35.0	40.0	30.0	41.0
50	35.95133821390134	38.0	35.0	40.0	30.0	41.0
51	34.72686362399915	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	9.0
9	11.0
10	16.0
11	10.0
12	9.0
13	11.0
14	18.0
15	31.0
16	53.0
17	76.0
18	114.0
19	259.0
20	370.0
21	613.0
22	1052.0
23	1429.0
24	2140.0
25	2946.0
26	3856.0
27	4458.0
28	5206.0
29	6355.0
30	7543.0
31	9918.0
32	13003.0
33	18654.0
34	33664.0
35	44762.0
36	38106.0
37	56953.0
38	99920.0
39	155655.0
40	97.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.73154510583105	21.6789075096882	22.916198518483476	11.673348865997264
2	33.11394431106328	24.339171880359064	24.23292688215281	18.313956926424847
3	31.6787498176686	24.62104636539606	26.447908412475012	17.25229540446032
4	29.543796987293963	25.67147233096401	24.887742993546453	19.89698768819557
5	23.325409309348377	31.866994666067438	24.56013782282513	20.247458201759056
6	25.4018189774461	33.0601319093744	25.277045166936713	16.26100394624279
7	77.47192096475978	6.76005976527543	10.111212296823687	5.656806973141107
8	76.99234011014788	6.225089588778636	10.0987940502801	6.683776250793389
9	71.00911065643245	8.169432190460421	12.585991429438733	8.235465723668389
10	39.81033592342476	25.446564088007918	20.064535459021755	14.678564529545572
11	30.553814372839128	25.13827618968773	23.90551882645599	20.40239061101715
12	26.858301893486924	23.27455363302702	29.677638088930415	20.189506384555646
13	24.696147189731096	26.904821039269255	30.322598449098987	18.076433321900662
14	20.53130383704107	29.831387808041505	28.468534528638838	21.168773826278585
15	17.754150256840877	26.150067610453405	36.48362565491467	19.61215647779105
16	20.72053426056241	27.183935913963236	31.44378870846294	20.651741117011422
17	21.141769067921896	25.460559254747515	27.73743490276316	25.66023677456743
18	24.054340669954545	24.292258504527734	30.465309726837997	21.188091098679724
19	23.043929054360383	26.645417667025416	27.795189604942067	22.515463673672134
20	25.204309722895697	26.414595973334283	28.339621302614926	20.041473001155094
21	22.68143452430231	27.657603317840092	29.357720404164645	20.303241753692948
22	22.92822253497806	24.9368246346473	28.043357420789327	24.09159540958531
23	20.54037112816813	27.12854659207834	28.37589046712318	23.955191812630343
24	22.167949885476173	24.392787167023442	29.603917069766894	23.835345877733495
25	20.418751158050767	27.450238312064624	27.919174955353448	24.21183557453116
26	20.229520734529427	27.09326300269259	27.47803153051932	25.19918473225866
27	20.906216613642727	27.462459443583707	28.068785258950008	23.562538683823558
28	17.271612676861455	27.449055621917612	31.03418368754903	24.245148013671898
29	19.71505052058078	26.986623774437334	28.331539586610372	24.966786118371516
30	20.703188138406286	25.129603128609666	28.602966975348792	25.56424175763525
31	22.91836678375299	26.69095123768524	26.167019502560525	24.223662476001245
32	21.73035453108307	28.501452737730578	25.02000717498689	24.748185556199466
33	21.217855467379433	27.301022238517064	24.91080545141312	26.570316842690385
34	20.165655466590973	26.143365699620357	28.77209166637099	24.918887167417676
35	18.76712436775356	27.57816596296603	25.527775478102495	28.12693419117792
36	21.275807284582847	27.548007364217312	25.916092076370244	25.260093274829593
37	20.41737135287926	27.15909942087606	28.40782310109241	24.015706125152274
38	18.846561722627623	29.693407290890526	25.745981810225537	25.714049176256314
39	21.13960080265238	26.27070200544826	27.246618491754678	25.343078700144684
40	24.294623884821746	22.893333175641313	27.994670009737483	24.817372929799454
41	19.80079555623889	25.31094895115095	26.278389491403814	28.609866001206345
42	21.832854343823797	24.369330479107777	26.835239435620263	26.962575741448163
43	21.975959851611808	25.4296121959008	27.350892339715916	25.243535612771478
44	20.842942690777775	24.788791251246753	27.821603018225254	26.546663039750218
45	20.47808278042569	24.304479636046818	26.713619465502898	28.503818118024594
46	22.049680870775333	24.497455245033688	27.320339510918203	26.13252437327278
47	19.505123019486792	25.361804627472317	29.63801796900563	25.49505438403526
48	19.682132311489045	25.14083868500625	28.132650526888458	27.04437847661624
49	21.700590162383357	22.973558990613384	30.131593990357132	25.19425685664613
50	19.811833997610968	24.132792449706102	29.39911455930994	26.656258993372994
51	19.575887313282795	23.470485967381403	26.11399556096965	30.839631158366153
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	398.0
1	617.5
2	837.0
3	1441.0
4	2045.0
5	1515.0
6	985.0
7	992.5
8	1000.0
9	1050.5
10	1101.0
11	1068.0
12	1035.0
13	1019.0
14	1003.0
15	978.5
16	954.0
17	1004.5
18	1055.0
19	1138.0
20	1221.0
21	1410.0
22	1599.0
23	1902.0
24	2205.0
25	2613.0
26	3253.0
27	3485.0
28	3747.5
29	4010.0
30	5331.5
31	6653.0
32	7612.0
33	8571.0
34	10512.5
35	12454.0
36	13902.0
37	15350.0
38	16328.5
39	17307.0
40	18822.0
41	20337.0
42	23212.5
43	26088.0
44	29996.0
45	33904.0
46	36014.0
47	38124.0
48	43965.5
49	49807.0
50	54456.5
51	59106.0
52	57782.0
53	56458.0
54	47566.0
55	38674.0
56	32559.0
57	26444.0
58	22535.5
59	18627.0
60	17548.0
61	16469.0
62	14031.0
63	11593.0
64	10261.0
65	8929.0
66	7480.5
67	6032.0
68	5177.5
69	4323.0
70	3303.0
71	2283.0
72	1867.0
73	1451.0
74	1217.0
75	836.5
76	690.0
77	524.0
78	358.0
79	282.0
80	206.0
81	150.0
82	94.0
83	59.0
84	24.0
85	16.5
86	9.0
87	8.0
88	7.0
89	6.0
90	5.0
91	3.5
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	507318.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.83832991919118
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.31685014507376	23.024477336355673
2	11.813639449987118	7.522531007101242
3	4.4980307435174804	4.29629360396323
4	2.1767313617761737	2.772139649667205
5	1.2709183731823523	2.023195923287074
6	0.8171027213946201	1.5609111612978523
7	0.5964492262661525	1.3292963072135655
8	0.4611721371877325	1.174636052265722
9	0.3712463313234051	1.0637876860167539
>10	5.116484040642412	40.121847868123645
>50	0.5013386222883052	9.819256268396716
>100	0.053847481653188325	3.020718443843008
>500	0.003713619424357816	0.8069568809144183
>1k	0.0024757462829052106	1.4639518115538983
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	2927	0.5769556767155906	No Hit
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	2021	0.3983694645173244	No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT	1438	0.28345140523300966	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1043	0.20559097055495765	No Hit
TCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	868	0.17109584126721306	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGC	680	0.13403821666095034	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCG	646	0.1273363058279028	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTC	640	0.12615361568089442	No Hit
ACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	631	0.12437958046038185	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCC	630	0.12418246543588045	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.9423004900279507E-4	0.0	0.0	0.3023744475851438	0.0
2	3.9423004900279507E-4	0.0	0.0	1.6502469851257002	0.0
3	3.9423004900279507E-4	0.0	0.0	2.2185296007632296	0.0
4	3.9423004900279507E-4	0.0	0.0	2.90271585080758	0.0
5	3.9423004900279507E-4	0.0	0.0	4.779053769036383	0.0
6	3.9423004900279507E-4	0.0	0.0	5.727571266937108	0.0
7	3.9423004900279507E-4	0.0	0.0	6.71649734486062	0.0
8	3.9423004900279507E-4	0.0	0.0	8.304061752194876	0.0
9	3.9423004900279507E-4	0.0	0.0	8.85322421045577	0.0
10	3.9423004900279507E-4	0.0	0.0	10.188678501452738	0.0
11	3.9423004900279507E-4	0.0	0.0	11.93097820302059	0.0
12	3.9423004900279507E-4	0.0	0.0	13.491143621949153	0.0
13	3.9423004900279507E-4	0.0	0.0	14.085839650869868	0.0
14	3.9423004900279507E-4	0.0	0.0	14.33578150193764	0.0
15	3.9423004900279507E-4	0.0	0.0	14.817924851868058	0.0
16	5.913450735041926E-4	0.0	0.0	15.696466516070789	0.0
17	5.913450735041926E-4	0.0	0.0	16.80129622840112	0.0
18	5.913450735041926E-4	0.0	0.0	18.102255390110344	0.0
19	5.913450735041926E-4	0.0	0.0	18.804576222408823	0.0
20	5.913450735041926E-4	0.0	0.0	19.51596434583437	0.0
21	5.913450735041926E-4	0.0	0.0	20.347001289132262	0.0
22	5.913450735041926E-4	0.0	0.0	21.20070646024781	0.0
23	5.913450735041926E-4	0.0	0.0	22.01991650207562	0.0
24	5.913450735041926E-4	0.0	0.0	22.678872028983793	0.0
25	5.913450735041926E-4	0.0	0.0	23.25523636062588	0.0
26	7.884600980055901E-4	0.0	0.0	23.817014180454862	0.0
27	9.855751225069877E-4	0.0	0.0	24.373272779597805	0.0
28	0.0011826901470083852	0.0	0.0	24.92381504303021	0.0
29	0.0011826901470083852	0.0	0.0	25.468049625678567	0.0
30	0.0011826901470083852	0.0	0.0	26.199149251554253	0.0
31	0.0015769201960111803	0.0	0.0	26.792071245254455	0.0
32	0.0015769201960111803	0.0	0.0	27.394060530081724	0.0
33	0.0015769201960111803	0.0	0.0	27.973578702115834	0.0
34	0.0015769201960111803	0.0	0.0	28.538116132287836	0.0
35	0.0015769201960111803	0.0	0.0	29.14917270824217	0.0
36	0.0015769201960111803	0.0	0.0	29.69281594581702	0.0
37	0.0015769201960111803	0.0	0.0	30.258930296185035	0.0
38	0.0015769201960111803	0.0	0.0	30.84751575934621	0.0
39	0.0015769201960111803	0.0	0.0	31.501346295617346	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATGT	70	0.0	45.000004	9
CATATCG	20	7.029557E-4	45.0	44
GGTCGGT	20	7.029557E-4	45.0	17
CGAAAGT	20	7.029557E-4	45.0	13
TAGACGA	25	3.8877137E-5	45.0	11
CCGGCAC	20	7.029557E-4	45.0	26
TAGCGGA	20	7.029557E-4	45.0	2
CCAATCG	20	7.029557E-4	45.0	24
TTGTACG	25	3.8877137E-5	45.0	1
TACGCTA	20	7.029557E-4	45.0	20
GTAGACG	25	3.8877137E-5	45.0	1
TACGACG	25	3.8877137E-5	45.0	27
CATACGG	25	3.8877137E-5	45.0	2
CTACCGA	20	7.029557E-4	45.0	27
GTATACG	20	7.029557E-4	45.0	1
TTACGCG	25	3.8877137E-5	45.0	1
TTGCACG	25	3.8877137E-5	45.0	1
CGCGTAA	20	7.029557E-4	45.0	18
ATACGAA	40	6.8012014E-9	45.0	19
TTCGCAA	25	3.8877137E-5	45.0	15
>>END_MODULE