##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935790.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1584927
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.124678297486255	33.0	31.0	34.0	30.0	34.0
2	32.21880313730538	33.0	31.0	34.0	30.0	34.0
3	32.123476349383914	33.0	31.0	34.0	30.0	34.0
4	35.835485798399546	37.0	35.0	37.0	35.0	37.0
5	34.90884879871439	37.0	35.0	37.0	33.0	37.0
6	35.41671761538544	37.0	35.0	37.0	33.0	37.0
7	35.98983297022513	37.0	35.0	37.0	35.0	37.0
8	36.00534977320722	37.0	35.0	37.0	35.0	37.0
9	37.88232139398218	39.0	38.0	39.0	35.0	39.0
10	37.5248172313299	39.0	37.0	39.0	35.0	39.0
11	37.47010240850209	39.0	37.0	39.0	35.0	39.0
12	37.49404294330275	39.0	37.0	39.0	35.0	39.0
13	37.52567342218285	39.0	37.0	39.0	35.0	39.0
14	38.72558988521238	40.0	38.0	41.0	35.0	41.0
15	38.81012374702431	40.0	38.0	41.0	35.0	41.0
16	38.78279630544498	40.0	38.0	41.0	35.0	41.0
17	38.76724227677363	40.0	38.0	41.0	35.0	41.0
18	38.59600536807058	40.0	38.0	41.0	35.0	41.0
19	38.38100492956458	40.0	37.0	41.0	34.0	41.0
20	38.25827372490973	40.0	36.0	41.0	34.0	41.0
21	38.17386542093106	40.0	36.0	41.0	34.0	41.0
22	38.21572539303072	40.0	36.0	41.0	34.0	41.0
23	38.21532600555104	40.0	36.0	41.0	34.0	41.0
24	38.17214420601075	40.0	36.0	41.0	34.0	41.0
25	38.03300909127045	40.0	36.0	41.0	34.0	41.0
26	38.059149727400694	40.0	36.0	41.0	34.0	41.0
27	38.07156228646493	40.0	36.0	41.0	34.0	41.0
28	37.95698035303834	40.0	36.0	41.0	34.0	41.0
29	37.894166103549246	40.0	36.0	41.0	34.0	41.0
30	37.69836907314974	40.0	36.0	41.0	33.0	41.0
31	37.53355391131579	40.0	36.0	41.0	33.0	41.0
32	37.24928908397674	40.0	35.0	41.0	33.0	41.0
33	36.842420502647755	40.0	35.0	41.0	31.0	41.0
34	36.55287972253612	40.0	35.0	41.0	30.0	41.0
35	36.370526844454034	40.0	35.0	41.0	29.0	41.0
36	36.23359372387498	40.0	35.0	41.0	29.0	41.0
37	36.16103706984612	40.0	35.0	41.0	28.0	41.0
38	36.07750767070029	40.0	35.0	41.0	27.0	41.0
39	36.00119248394405	40.0	35.0	41.0	26.0	41.0
40	35.92467413325661	40.0	35.0	41.0	26.0	41.0
41	35.82975178036591	39.0	35.0	41.0	25.0	41.0
42	35.81662057621581	39.0	35.0	41.0	25.0	41.0
43	35.724500245121696	39.0	35.0	41.0	24.0	41.0
44	35.617777348735935	39.0	35.0	41.0	24.0	41.0
45	35.572319734599766	39.0	35.0	41.0	23.0	41.0
46	35.47395116620513	39.0	35.0	41.0	23.0	41.0
47	35.40364319618506	39.0	35.0	41.0	23.0	41.0
48	35.331212099989465	39.0	35.0	41.0	23.0	41.0
49	35.31207620287874	39.0	35.0	41.0	23.0	41.0
50	35.21791666114591	39.0	35.0	41.0	23.0	41.0
51	34.19228267295591	38.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	9.0
9	25.0
10	42.0
11	38.0
12	23.0
13	44.0
14	31.0
15	50.0
16	104.0
17	182.0
18	380.0
19	785.0
20	1358.0
21	2237.0
22	3500.0
23	5860.0
24	10329.0
25	18645.0
26	27881.0
27	30513.0
28	27955.0
29	24855.0
30	24808.0
31	27531.0
32	33437.0
33	44333.0
34	73856.0
35	97215.0
36	103126.0
37	152488.0
38	293872.0
39	579015.0
40	399.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.76154990103645	19.830061573813808	21.889651700046752	20.518736825102984
2	40.32173090621839	21.894446873578406	22.183545362026138	15.600276858177065
3	28.348876635958625	21.553169325779674	34.78097098478353	15.316983053478172
4	24.939634443731478	23.435779692061526	34.591498535894715	17.033087328312284
5	22.20670100263293	28.755141403988954	32.02185337242662	17.0163042209515
6	23.32940255292515	30.96243549387448	31.791685042907343	13.91647691029303
7	72.63886601717303	4.9874221336377005	17.518787931557732	4.854923917631537
8	74.75921603960309	4.2417095550773	16.530540523317477	4.468533882002136
9	68.50271337418064	6.483326992347281	18.4806618853739	6.533297748098176
10	32.33751459846415	27.86967475473634	25.790399179268192	14.002411467531312
11	23.130655228915906	23.913214930403733	34.515595986439756	18.440533854240606
12	20.531292608429283	22.066252893666398	37.36538023517802	20.037074262726296
13	20.875914158822457	21.968835157707577	39.324334811634856	17.830915871835106
14	18.507792472460878	25.5076101296779	37.23338677428046	18.75121062358077
15	17.74883007229986	25.869771920094742	38.69143499984542	17.689963007759978
16	20.28005075312617	26.34310602317962	35.900454721258455	17.476388502435757
17	20.832631408260443	25.67752331810866	34.14750332349692	19.34234195013398
18	20.836290882797755	25.219331868281632	35.0084262555941	18.935950993326507
19	19.768102884233784	27.141250038645314	33.86282144224939	19.22782563487151
20	20.307181340213145	26.552074638137906	33.9536142674079	19.18712975424105
21	20.769726302851804	26.484500547974765	35.410842265921396	17.334930883252035
22	20.283836416440632	23.686201320313174	35.408507773544144	20.62145448970205
23	18.569372595709453	25.70995383383588	35.32919812710617	20.391475443348494
24	19.199243876847323	24.731801527767523	35.87117892495995	20.1977756704252
25	20.178027126801425	26.467717440613985	33.56589924961844	19.788356182966155
26	18.97645759079125	26.755238569347355	33.846543089997205	20.42176074986419
27	19.48802689335219	25.544331063828174	34.210786995237	20.756855047582633
28	18.988130052677505	25.6090028121169	34.42877810776143	20.974089027444165
29	18.890964694272984	25.015221521243564	34.833402421688824	21.260411362794628
30	19.22561733127141	26.014825919427203	34.69768639186537	20.06187035743602
31	19.160251544708366	27.003388799610327	32.59809442327627	21.238265232405027
32	19.11690569975778	27.832133593534593	32.30224483525109	20.74871587145654
33	19.141007756193186	28.50478287012588	31.320685432199717	21.033523941481217
34	18.205948917521123	27.6354683843483	32.31858628189185	21.83999641623873
35	19.398874522296612	27.68089634412184	31.311852217799306	21.60837691578224
36	20.139728832936786	27.82778008072296	31.561705996553783	20.47078508978647
37	19.524369261171017	27.602974774232504	32.124634131414254	20.74802183318222
38	19.659959102217325	27.784055669440928	31.45318364820588	21.102801580135868
39	19.80690593320702	26.51043234167883	31.59382104033814	22.088840684776017
40	21.195865803283056	25.949333944087016	32.27719636298706	20.577603889642866
41	19.533833419457174	26.516867969313413	32.096430939721515	21.8528676715079
42	20.169698667509607	26.71290223461396	32.43278712521144	20.68461197266499
43	20.397469410263056	26.31105407378384	32.147285016912456	21.144191499040648
44	20.572808716111215	26.417115740977344	30.640212451425207	22.369863091486234
45	20.047421742452492	25.864598180231646	30.56191231520442	23.526067762111442
46	20.70505455456308	25.770082786147245	30.680340482558506	22.844522176731168
47	19.88230372755338	25.813176253543542	32.14924094295826	22.155279075944822
48	19.158232524273988	25.540671589290863	32.68346113101739	22.617634755417757
49	19.946786192676385	24.721138576098458	32.53777618779918	22.794299043425976
50	18.664392744902447	24.80909215377112	33.4956121007466	23.03090300057984
51	18.79613382824572	24.19840156675986	32.33770388162988	24.667760723364545
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	575.0
1	1014.0
2	1453.0
3	21942.5
4	42432.0
5	28410.0
6	14388.0
7	13961.5
8	13535.0
9	13579.0
10	13623.0
11	13465.5
12	13308.0
13	12818.5
14	12329.0
15	11842.5
16	11356.0
17	10788.5
18	10221.0
19	9594.5
20	8968.0
21	9316.5
22	9665.0
23	9982.5
24	10300.0
25	11015.0
26	13258.5
27	14787.0
28	17991.5
29	21196.0
30	24347.5
31	27499.0
32	31478.5
33	35458.0
34	41064.5
35	46671.0
36	51563.0
37	56455.0
38	61747.5
39	67040.0
40	74663.5
41	82287.0
42	92471.5
43	102656.0
44	128733.0
45	154810.0
46	159089.0
47	163368.0
48	157672.0
49	151976.0
50	134225.5
51	116475.0
52	104141.5
53	91808.0
54	82272.0
55	72736.0
56	65313.0
57	57890.0
58	52299.0
59	46708.0
60	41437.5
61	36167.0
62	31409.5
63	26652.0
64	21975.0
65	17298.0
66	13380.0
67	9462.0
68	7349.0
69	5236.0
70	4058.0
71	2880.0
72	2290.0
73	1700.0
74	1303.5
75	705.5
76	504.0
77	389.5
78	275.0
79	180.0
80	85.0
81	58.0
82	31.0
83	21.5
84	12.0
85	9.0
86	6.0
87	4.5
88	3.0
89	3.0
90	3.0
91	2.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1584927.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.10717382810879
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.73336085525483	24.74210368593703
2	9.016262992250967	5.9700597232879415
3	3.447464958129213	3.4240746490529292
4	1.9965928905945438	2.6440619157151906
5	1.3346038891144303	2.209248147429074
6	1.0362489380526203	2.0584364232780743
7	0.8085467663506315	1.8738088839207936
8	0.6661758465320884	1.76441596409803
9	0.5986249533445527	1.783690234939946
>10	6.135013279781084	41.71606556241145
>50	0.18601747867524224	3.826299585766171
>100	0.03660846204222501	2.174899254685326
>500	0.0013630795279586227	0.32234079195473286
>1k	0.00214198211536355	1.8307022938277098
>5k	5.841769405536955E-4	1.310637660087944
>10k+	3.8945129370246363E-4	2.3491552236077022
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25184	1.588969081856767	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT	11255	0.7101273434044596	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTC	8468	0.534283282447709	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCT	6513	0.41093375278482863	No Hit
CGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG	5349	0.33749188448426964	No Hit
CGTCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCT	4106	0.259065559486336	No Hit
CCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC	3150	0.1987473240092446	No Hit
GAATCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTC	3102	0.19571879335767514	No Hit
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC	2957	0.18657010701439244	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTC	2880	0.1817118390941665	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCC	2645	0.1668846577791911	No Hit
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCT	2312	0.1458742263839281	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGT	2173	0.13710410637209158	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCG	1785	0.1126234836052386	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGC	1654	0.104358118701997	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCG	1633	0.10303313654193537	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.892831657230901E-4	0.0	0.0	0.1517420045213439	0.0
2	1.892831657230901E-4	0.0	0.0	0.6470960492186706	0.0
3	1.892831657230901E-4	0.0	0.0	1.2469974957837175	0.0
4	1.892831657230901E-4	0.0	0.0	1.910939746751743	0.0
5	1.892831657230901E-4	0.0	0.0	3.533727420884369	0.0
6	1.892831657230901E-4	0.0	0.0	5.422899603577957	0.0
7	1.892831657230901E-4	0.0	0.0	6.677279142824875	0.0
8	1.892831657230901E-4	0.0	0.0	8.024218150110384	0.0
9	1.892831657230901E-4	0.0	0.0	8.778763943071196	0.0
10	1.892831657230901E-4	0.0	0.0	9.89036088097433	0.0
11	1.892831657230901E-4	0.0	0.0	10.96807613221303	0.0
12	1.892831657230901E-4	0.0	0.0	11.897645759079126	0.0
13	1.892831657230901E-4	0.0	0.0	12.342335009751238	0.0
14	1.892831657230901E-4	0.0	0.0	12.5813996480595	0.0
15	1.892831657230901E-4	0.0	0.0	12.854787633752217	0.0
16	1.892831657230901E-4	0.0	0.0	13.289571065418155	0.0
17	2.5237755429745345E-4	0.0	0.0	13.841457682278111	0.0
18	2.5237755429745345E-4	0.0	0.0	14.471770624136001	0.0
19	2.5237755429745345E-4	0.0	0.0	14.883209132029425	0.0
20	3.154719428718168E-4	0.0	0.0	15.285057292859545	0.0
21	3.154719428718168E-4	0.0	0.0	15.750946258092643	0.0
22	3.785663314461802E-4	0.0	0.0	16.260622728996353	0.0
23	3.785663314461802E-4	0.0	0.0	16.78146690667772	0.0
24	3.785663314461802E-4	0.0	0.0	17.20748021833182	0.0
25	3.785663314461802E-4	0.0	0.0	17.590967912086803	0.0
26	3.785663314461802E-4	0.0	0.0	17.96152125618404	0.0
27	3.785663314461802E-4	0.0	0.0	18.35371597556228	0.0
28	4.4166072002054354E-4	0.0	0.0	18.753292738403726	0.0
29	4.4166072002054354E-4	0.0	0.0	19.185552394526688	0.0
30	4.4166072002054354E-4	0.0	0.0	19.726839154106152	0.0
31	4.4166072002054354E-4	0.0	0.0	20.161244019440645	0.0
32	4.4166072002054354E-4	0.0	0.0	20.595964356718007	0.0
33	4.4166072002054354E-4	0.0	0.0	21.01491109685178	0.0
34	4.4166072002054354E-4	0.0	0.0	21.434173308928425	0.0
35	4.4166072002054354E-4	0.0	0.0	21.856274768490916	0.0
36	4.4166072002054354E-4	0.0	0.0	22.274906036681816	0.0
37	4.4166072002054354E-4	0.0	0.0	22.691644473215486	0.0
38	4.4166072002054354E-4	0.0	0.0	23.13071832330448	0.0
39	4.4166072002054354E-4	0.0	0.0	23.635599620676537	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGTAA	20	7.034096E-4	45.000004	27
GTTGCGA	20	7.034096E-4	45.000004	16
CGACGGT	50	2.1827873E-11	45.0	28
GTTCGCG	30	2.1660253E-6	44.999996	1
CGTTTTT	15480	0.0	44.127907	1
CGTTTCT	1240	0.0	41.008064	1
CGTTCTG	955	0.0	40.759163	1
CGTTATT	455	0.0	40.54945	1
GCGAAAG	130	0.0	39.807693	1
GGCGTAG	105	0.0	38.57143	1
TAACGCG	35	6.2497893E-6	38.571426	1
GCGTTAT	35	6.2497893E-6	38.571426	19
CGTTTTC	860	0.0	38.197674	1
GTTTTTT	17955	0.0	37.84461	2
TCGAACG	30	1.1401173E-4	37.499996	1
TCGCGCG	30	1.1401173E-4	37.499996	2
CTACGAA	145	0.0	37.24138	11
TACGAAT	145	0.0	37.24138	12
CGTGCGG	195	0.0	36.923077	2
CGCGAGG	135	0.0	36.666668	2
>>END_MODULE