FastQCFastQC Report
Sat 14 Jan 2017
SRR2935789.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935789.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1385497
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188691.3618939629605837No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTT72040.5199578201901556TruSeq Adapter, Index 14 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTC60790.43875952095168735No Hit
CGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG50290.3629744416624504Illumina PCR Primer Index 3 (95% over 22bp)
CGTTTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCT40190.29007641301280335No Hit
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC31310.22598388881390577TruSeq Adapter, Index 14 (96% over 25bp)
CGTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCT30820.22244725178040803Illumina PCR Primer Index 3 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCC20440.14752828768304801No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTC17950.12955639745160039No Hit
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT17930.1294120449196209Illumina PCR Primer Index 3 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC16570.11959607274501496TruSeq Adapter, Index 14 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTT207.0337893E-445.027
TATAGCG453.8562575E-1045.01
CGTTTTT117300.044.136831
CGACGGT700.041.78571328
CGTTATT3500.041.7857131
ACGCGAG451.9288564E-840.01
TAGTGCG403.4588993E-739.3751
CGTTTCT9550.038.6387441
TCGATAG700.038.5714261
TCGGACG356.24922E-638.5714261
ACGTGTA356.24922E-638.57142632
CTACGAA2000.038.2511
TCGACGG900.037.52
CGAATAT2050.037.31707414
GTCTACG552.750312E-936.818181
GTTTTTT140850.036.7412152
CGTTCTG7300.036.678081
CGTAAGG2150.036.6279032
GCTAGCG1050.036.428571
GCACGCG751.8189894E-1236.0000041
Produced by FastQC (version 0.11.5)