##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935789.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1385497
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19045873069375	33.0	31.0	34.0	30.0	34.0
2	32.25748449834247	34.0	31.0	34.0	30.0	34.0
3	32.19683333850597	33.0	31.0	34.0	30.0	34.0
4	35.88461613413815	37.0	35.0	37.0	35.0	37.0
5	34.96619119348508	37.0	35.0	37.0	33.0	37.0
6	35.47310315359759	37.0	35.0	37.0	33.0	37.0
7	36.05089437220001	37.0	35.0	37.0	35.0	37.0
8	36.07494061697716	37.0	35.0	37.0	35.0	37.0
9	37.95514966831397	39.0	38.0	39.0	35.0	39.0
10	37.60920016427318	39.0	37.0	39.0	35.0	39.0
11	37.51639231265026	39.0	37.0	39.0	35.0	39.0
12	37.575166167808376	39.0	37.0	39.0	35.0	39.0
13	37.60287030574588	39.0	37.0	39.0	35.0	39.0
14	38.835633711224204	40.0	38.0	41.0	35.0	41.0
15	38.92824957397959	40.0	38.0	41.0	35.0	41.0
16	38.89173560101538	40.0	38.0	41.0	35.0	41.0
17	38.87398673544584	40.0	38.0	41.0	35.0	41.0
18	38.72510586453814	40.0	38.0	41.0	35.0	41.0
19	38.53160995657154	40.0	37.0	41.0	35.0	41.0
20	38.44171297375599	40.0	37.0	41.0	35.0	41.0
21	38.33807435165865	40.0	37.0	41.0	34.0	41.0
22	38.37862442141701	40.0	37.0	41.0	35.0	41.0
23	38.367237893694465	40.0	37.0	41.0	35.0	41.0
24	38.343918463915834	40.0	37.0	41.0	35.0	41.0
25	38.194865091732424	40.0	36.0	41.0	34.0	41.0
26	38.21970022309684	40.0	37.0	41.0	34.0	41.0
27	38.22787274169486	40.0	37.0	41.0	34.0	41.0
28	38.137758508318676	40.0	36.0	41.0	34.0	41.0
29	38.06559306876883	40.0	36.0	41.0	34.0	41.0
30	37.9039377205436	40.0	36.0	41.0	34.0	41.0
31	37.77923950755577	40.0	36.0	41.0	33.0	41.0
32	37.54223718997587	40.0	36.0	41.0	33.0	41.0
33	37.192551842407454	40.0	36.0	41.0	32.0	41.0
34	36.93308394027558	40.0	36.0	41.0	31.0	41.0
35	36.737317366980946	40.0	36.0	41.0	30.0	41.0
36	36.6169461211392	40.0	36.0	41.0	30.0	41.0
37	36.560012039001165	40.0	36.0	41.0	30.0	41.0
38	36.44614098767446	40.0	35.0	41.0	30.0	41.0
39	36.35608666059905	40.0	35.0	41.0	29.0	41.0
40	36.214873796190105	40.0	35.0	41.0	28.0	41.0
41	36.094956539061435	40.0	35.0	41.0	27.0	41.0
42	36.07170856378614	40.0	35.0	41.0	27.0	41.0
43	35.9627909695943	39.0	35.0	41.0	26.0	41.0
44	35.79338966450306	39.0	35.0	41.0	25.0	41.0
45	35.72688861830809	39.0	35.0	41.0	25.0	41.0
46	35.59182661528679	39.0	35.0	41.0	24.0	41.0
47	35.49270622744041	39.0	35.0	41.0	24.0	41.0
48	35.41723006256961	39.0	35.0	41.0	23.0	41.0
49	35.38272042451192	39.0	35.0	41.0	24.0	41.0
50	35.282894152784166	39.0	35.0	41.0	24.0	41.0
51	34.09983060230372	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	9.0
9	26.0
10	27.0
11	33.0
12	21.0
13	21.0
14	32.0
15	45.0
16	88.0
17	154.0
18	315.0
19	651.0
20	1102.0
21	1677.0
22	2774.0
23	4451.0
24	7821.0
25	14281.0
26	21562.0
27	24133.0
28	22833.0
29	20393.0
30	20547.0
31	22914.0
32	27822.0
33	37165.0
34	62273.0
35	82941.0
36	88378.0
37	136005.0
38	263509.0
39	521029.0
40	462.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.655219751468245	20.98063005549633	24.102397912084978	19.261752280950446
2	38.70740968764277	22.43967327247912	23.134658537694417	15.718258502183694
3	29.057226396015295	21.950823422930544	33.41508498394439	15.576865197109774
4	25.61340804058038	24.032892167936847	33.03052983875101	17.323169952731764
5	23.509109005649236	28.940878255239816	30.582888306506618	16.96712443260433
6	24.27735318084413	30.575237622311707	30.419048182709886	14.728361014134277
7	76.19265866328112	4.292106009612435	14.994907964434423	4.520327362672023
8	78.22239961544486	3.692609944301575	14.274661006122713	3.810329434130857
9	71.92437082144531	6.655517839446784	16.065281989062406	5.354829350045507
10	32.29418757312358	32.99624611240588	22.016142943651268	12.693423370819279
11	21.613976789556382	24.67764275202328	35.729994363033626	17.978386095386707
12	20.686078713992163	22.745844992807633	37.10069383044496	19.467382462755243
13	20.548366398483722	23.155517478565454	38.4565971633284	17.83951895962243
14	18.025517197078017	26.471511666932518	36.04908563497431	19.453885501015158
15	17.696249071632778	26.359710630914396	37.50553050638146	18.438509791071365
16	20.53133279971014	26.901754388497412	34.94219041975551	17.624722392036936
17	20.744252784379903	25.894823301674418	33.72075147041098	19.640172443534702
18	21.4279785521008	24.94065306528993	34.62071733103716	19.010651051572108
19	19.983803645911898	27.53546200388741	33.06199869072253	19.418735659478152
20	21.069045981333772	27.278297968165937	33.17459366566655	18.478062384833745
21	20.82566761241634	26.932790182873006	34.6915944242391	17.549947780471555
22	20.932560662347157	24.02682936159371	34.453268393940945	20.587341582118185
23	18.21671212568486	26.790819467671167	34.57452452080372	20.41794388584024
24	19.10772812932832	25.489986625737913	35.12891041987099	20.273374825062778
25	19.791598249581195	27.656501601952222	32.78628535464169	19.765614793824888
26	19.712709590854402	27.31987149737603	32.57820117979324	20.389217731976323
27	19.483189065006997	26.272666054130756	33.6529779566466	20.591166924215642
28	18.442190780636842	26.353792177103237	34.567090365406784	20.636926676853143
29	18.97456291857723	26.41290453894884	34.105739673200304	20.506792869273625
30	20.03367744571082	25.269632485671206	34.664383971960966	20.032306096657013
31	20.52541434589898	25.93978911538603	33.74110517742009	19.7936913612949
32	20.711268230822586	26.143037480413167	33.668640206366376	19.477054082397867
33	20.506865045539616	25.13300281415261	33.0383248754779	21.321807264829875
34	20.119711554770596	25.744119258287817	33.070226785045364	21.065942401896216
35	20.14208619722742	25.90911420234039	33.12832867916711	20.820470921265077
36	20.149881233954314	26.39341694713161	31.908910665270295	21.547791153643782
37	20.07301351067523	26.378115578741777	32.49223924699945	21.056631663583538
38	20.074529212261016	26.39363347592958	32.1813760693816	21.350461242427805
39	21.158977608757002	25.112360402079542	30.831752071639272	22.89690991752418
40	22.210297099163697	24.953500440636102	32.17480802917653	20.66139443102367
41	19.98647416775352	26.127086525629434	31.887329961739365	21.999109344877688
42	20.3809174613875	26.313012586819024	31.938430758060104	21.367639193733368
43	20.57471073556998	25.93502548183071	31.910426366856083	21.57983741574323
44	21.149089460316407	25.738633862072604	30.769103072760174	22.343173604850822
45	21.10527846686063	24.938559953576224	30.680759323188717	23.275402256374427
46	21.192467396176244	25.551264275563206	30.073323868618985	23.182944459641558
47	20.08210772018994	25.610376637408816	32.48891913876392	21.818596503637323
48	19.694665524356964	25.23202865109055	32.82338395536043	22.249921869192065
49	20.554357028560872	23.93487679872277	32.46004863236802	23.050717540348337
50	19.32382386970163	24.311059497061343	33.502490442057976	22.86262619117905
51	19.533062864805913	23.625384970158724	32.0032450449189	24.83830712011646
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	506.0
1	927.0
2	1348.0
3	16608.0
4	31868.0
5	21503.5
6	11139.0
7	10991.0
8	10843.0
9	10691.0
10	10539.0
11	10331.0
12	10123.0
13	9763.0
14	9403.0
15	9041.5
16	8680.0
17	8216.5
18	7753.0
19	7436.0
20	7119.0
21	7427.5
22	7736.0
23	8475.0
24	9214.0
25	9959.5
26	13155.0
27	15605.0
28	17657.0
29	19709.0
30	23029.5
31	26350.0
32	30827.5
33	35305.0
34	40509.5
35	45714.0
36	49677.0
37	53640.0
38	57011.5
39	60383.0
40	65749.0
41	71115.0
42	77518.0
43	83921.0
44	91162.0
45	98403.0
46	114054.5
47	129706.0
48	134851.5
49	139997.0
50	134044.5
51	128092.0
52	110816.5
53	93541.0
54	81255.5
55	68970.0
56	61016.0
57	53062.0
58	46326.5
59	39591.0
60	34727.5
61	29864.0
62	25473.5
63	21083.0
64	17513.0
65	13943.0
66	11374.5
67	8806.0
68	6489.0
69	4172.0
70	3568.0
71	2964.0
72	2586.5
73	2209.0
74	1805.0
75	920.5
76	440.0
77	340.0
78	240.0
79	217.5
80	195.0
81	112.5
82	30.0
83	42.0
84	54.0
85	31.0
86	8.0
87	5.5
88	3.0
89	2.5
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1385497.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.12216627042905
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.9570171215167	23.756596008423276
2	9.170502277118931	5.8915279785792505
3	3.5241054591343994	3.3960570453852563
4	2.0160640701760117	2.5904138109612713
5	1.3999228233531902	2.2484276848759825
6	1.0576625766055383	2.0384647886240104
7	0.8437666241783452	1.8972528256706798
8	0.7387966148656411	1.8985398162195406
9	0.5932237186541699	1.715006783355432
>10	6.362842194340904	41.968914714058386
>50	0.29059106422967107	5.69387836507491
>100	0.041598421969572474	2.449880591764931
>500	6.894765629853646E-4	0.15111964396320438
>1k	0.002298255209951215	1.559034998150928
>5k	6.894765629853646E-4	1.3518822277744007
>10k+	2.2982552099512152E-4	1.3930027171185653
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18869	1.3618939629605837	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTT	7204	0.5199578201901556	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTC	6079	0.43875952095168735	No Hit
CGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG	5029	0.3629744416624504	Illumina PCR Primer Index 3 (95% over 22bp)
CGTTTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCT	4019	0.29007641301280335	No Hit
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	3131	0.22598388881390577	TruSeq Adapter, Index 14 (96% over 25bp)
CGTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCT	3082	0.22244725178040803	Illumina PCR Primer Index 3 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCC	2044	0.14752828768304801	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTC	1795	0.12955639745160039	No Hit
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT	1793	0.1294120449196209	Illumina PCR Primer Index 3 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	1657	0.11959607274501496	TruSeq Adapter, Index 14 (96% over 25bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.774101279179962E-4	0.0	0.0	0.13865060696630885	0.0
2	5.774101279179962E-4	0.0	0.0	0.6046205801961317	0.0
3	5.774101279179962E-4	0.0	0.0	1.224975586378029	0.0
4	5.774101279179962E-4	0.0	0.0	1.7838364139366596	0.0
5	5.774101279179962E-4	0.0	0.0	3.13512046579675	0.0
6	5.774101279179962E-4	0.0	0.0	4.6747123956240975	0.0
7	5.774101279179962E-4	0.0	0.0	5.735920034471385	0.0
8	5.774101279179962E-4	0.0	0.0	6.980022331336698	0.0
9	5.774101279179962E-4	0.0	0.0	7.665624681973328	0.0
10	5.774101279179962E-4	0.0	0.0	8.66490508460141	0.0
11	5.774101279179962E-4	0.0	0.0	9.778223987493297	0.0
12	5.774101279179962E-4	0.0	0.0	10.643545240444404	0.0
13	5.774101279179962E-4	0.0	0.0	11.091326794644809	0.0
14	6.495863939077458E-4	0.0	0.0	11.335715631286101	0.0
15	6.495863939077458E-4	0.0	0.0	11.591580494219764	0.0
16	6.495863939077458E-4	0.0	0.0	12.022472802178568	0.0
17	6.495863939077458E-4	0.0	0.0	12.579673575619434	0.0
18	6.495863939077458E-4	0.0	0.0	13.22370239704597	0.0
19	6.495863939077458E-4	0.0	0.0	13.630704360962167	0.0
20	6.495863939077458E-4	0.0	0.0	14.010856753930177	0.0
21	6.495863939077458E-4	0.0	0.0	14.473723147722442	0.0
22	6.495863939077458E-4	0.0	0.0	14.99267050018874	0.0
23	6.495863939077458E-4	0.0	0.0	15.513422259304784	0.0
24	6.495863939077458E-4	0.0	0.0	15.922300806136715	0.0
25	6.495863939077458E-4	0.0	0.0	16.305484602276294	0.0
26	6.495863939077458E-4	0.0	0.0	16.660086597083936	0.0
27	6.495863939077458E-4	0.0	0.0	17.057128236293547	0.0
28	6.495863939077458E-4	0.0	0.0	17.443054730540737	0.0
29	6.495863939077458E-4	0.0	0.0	17.87199827931782	0.0
30	6.495863939077458E-4	0.0	0.0	18.41000016600541	0.0
31	6.495863939077458E-4	0.0	0.0	18.852512852788568	0.0
32	6.495863939077458E-4	0.0	0.0	19.2979847664773	0.0
33	6.495863939077458E-4	0.0	0.0	19.729237955766052	0.0
34	7.217626598974953E-4	0.0	0.0	20.14288013615331	0.0
35	7.217626598974953E-4	0.0	0.0	20.591166924215642	0.0
36	7.217626598974953E-4	0.0	0.0	21.00350993181508	0.0
37	7.217626598974953E-4	0.0	0.0	21.428050728366788	0.0
38	7.217626598974953E-4	0.0	0.0	21.867676364510352	0.0
39	7.217626598974953E-4	0.0	0.0	22.34974164505589	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGTT	20	7.0337893E-4	45.0	27
TATAGCG	45	3.8562575E-10	45.0	1
CGTTTTT	11730	0.0	44.13683	1
CGACGGT	70	0.0	41.785713	28
CGTTATT	350	0.0	41.785713	1
ACGCGAG	45	1.9288564E-8	40.0	1
TAGTGCG	40	3.4588993E-7	39.375	1
CGTTTCT	955	0.0	38.638744	1
TCGATAG	70	0.0	38.571426	1
TCGGACG	35	6.24922E-6	38.571426	1
ACGTGTA	35	6.24922E-6	38.571426	32
CTACGAA	200	0.0	38.25	11
TCGACGG	90	0.0	37.5	2
CGAATAT	205	0.0	37.317074	14
GTCTACG	55	2.750312E-9	36.81818	1
GTTTTTT	14085	0.0	36.741215	2
CGTTCTG	730	0.0	36.67808	1
CGTAAGG	215	0.0	36.627903	2
GCTAGCG	105	0.0	36.42857	1
GCACGCG	75	1.8189894E-12	36.000004	1
>>END_MODULE