##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935788.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1375415
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.026701032052145	33.0	31.0	34.0	30.0	34.0
2	32.047930261048485	33.0	31.0	34.0	30.0	34.0
3	32.10153735418037	33.0	31.0	34.0	30.0	34.0
4	35.7696433440089	37.0	35.0	37.0	35.0	37.0
5	34.780173256798854	37.0	35.0	37.0	33.0	37.0
6	35.268537132429124	37.0	35.0	37.0	33.0	37.0
7	35.83235968780332	37.0	35.0	37.0	35.0	37.0
8	35.807586801074585	37.0	35.0	37.0	35.0	37.0
9	37.69371498784003	39.0	37.0	39.0	35.0	39.0
10	37.36018947008721	39.0	37.0	39.0	34.0	39.0
11	37.25557522638622	39.0	37.0	39.0	34.0	39.0
12	37.10731888193745	39.0	37.0	39.0	34.0	39.0
13	36.95955111729914	39.0	37.0	39.0	33.0	39.0
14	37.86793876757197	40.0	37.0	41.0	33.0	41.0
15	37.951933052933114	40.0	37.0	41.0	33.0	41.0
16	38.10943751522268	40.0	37.0	41.0	33.0	41.0
17	38.07936877233417	40.0	37.0	41.0	33.0	41.0
18	38.06274688003257	40.0	37.0	41.0	33.0	41.0
19	38.00019557733484	40.0	37.0	41.0	33.0	41.0
20	37.916975603726875	40.0	36.0	41.0	33.0	41.0
21	37.88950098697484	40.0	36.0	41.0	33.0	41.0
22	37.96454379223725	40.0	36.0	41.0	34.0	41.0
23	37.979659230123275	40.0	36.0	41.0	34.0	41.0
24	37.922265643460335	40.0	36.0	41.0	34.0	41.0
25	37.81611659026548	40.0	36.0	41.0	33.0	41.0
26	37.79844846828048	40.0	36.0	41.0	33.0	41.0
27	37.81056917366758	40.0	36.0	41.0	34.0	41.0
28	37.69046360552997	40.0	36.0	41.0	33.0	41.0
29	37.61464067208806	40.0	36.0	41.0	33.0	41.0
30	37.503259016369604	40.0	35.0	41.0	33.0	41.0
31	37.44147257373229	40.0	35.0	41.0	33.0	41.0
32	37.35055019757673	40.0	35.0	41.0	33.0	41.0
33	37.19020877335204	40.0	35.0	41.0	32.0	41.0
34	37.05896620292784	40.0	35.0	41.0	32.0	41.0
35	37.0373610873809	40.0	35.0	41.0	32.0	41.0
36	36.99045306325727	39.0	35.0	41.0	31.0	41.0
37	36.98758847329715	39.0	35.0	41.0	32.0	41.0
38	36.81079456018729	39.0	35.0	41.0	31.0	41.0
39	36.76636287956726	39.0	35.0	41.0	31.0	41.0
40	36.56254366863819	39.0	35.0	41.0	31.0	41.0
41	36.430732542541705	39.0	35.0	41.0	30.0	41.0
42	36.58201488278083	39.0	35.0	41.0	31.0	41.0
43	36.50311069749857	39.0	35.0	41.0	31.0	41.0
44	36.40748428656078	39.0	35.0	41.0	31.0	41.0
45	36.38697556737421	39.0	35.0	41.0	31.0	41.0
46	36.27940003562561	38.0	35.0	40.0	31.0	41.0
47	36.131345084938	38.0	35.0	40.0	30.0	41.0
48	36.11027289945216	38.0	35.0	40.0	30.0	41.0
49	36.11894591814107	38.0	35.0	40.0	30.0	41.0
50	35.980276498365946	38.0	35.0	40.0	30.0	41.0
51	34.84121083454812	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	17.0
9	22.0
10	34.0
11	34.0
12	38.0
13	26.0
14	43.0
15	70.0
16	115.0
17	194.0
18	399.0
19	731.0
20	1388.0
21	2340.0
22	3415.0
23	4888.0
24	6422.0
25	8540.0
26	10401.0
27	12492.0
28	14308.0
29	16783.0
30	20942.0
31	27429.0
32	37750.0
33	54949.0
34	95273.0
35	106554.0
36	101493.0
37	153270.0
38	261631.0
39	433097.0
40	325.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.86112918646372	19.642726013603166	22.69649523961859	10.799649560314522
2	31.031361443637014	27.810878898368856	23.58517247521657	17.57258718277756
3	31.402594853189765	28.306656536390832	24.92513168752704	15.365616922892364
4	27.834071898299783	23.602621754161472	29.284834031910368	19.278472315628374
5	27.379372771127258	29.56060534456873	24.09992620409113	18.96009568021288
6	24.835340606289737	36.362334277290856	24.454364682659417	14.347960433759994
7	77.13206559474777	5.680685465841219	12.557519003355349	4.629729936055663
8	77.51718572212751	8.317707746389273	9.03916272543196	5.125943806051265
9	72.3472551920693	6.229174467342584	10.55034298738926	10.873227353198853
10	43.09586561146999	23.14952214422556	18.021397178306184	15.733215065998262
11	32.544650160133486	23.04257260535911	23.871922292544433	20.540854941962973
12	28.16589901956864	20.093571758342026	29.29850263375054	22.44202658833879
13	26.908024123628145	21.533791619256736	33.04820726835173	18.50997698876339
14	21.910477928479768	26.45848707481015	30.13388686323764	21.497148133472443
15	19.082822275458682	23.834188226826086	36.106775046077	20.976214451638235
16	24.18760883078925	24.574183064747732	28.160373414569424	23.077834689893596
17	24.79295339951942	24.44753038173933	27.344910445211077	23.41460577353017
18	25.34602283674382	23.420131378529387	29.380586950120506	21.853258834606283
19	25.0517116652065	26.02036476263528	26.042176361316404	22.88574721084182
20	25.52778615908653	26.915876299153346	28.349552680463713	19.20678486129641
21	24.717485268082722	27.606504218726712	29.143058640483055	18.53295187270751
22	23.34626276432931	22.264552880403368	30.164932038693777	24.224252316573544
23	21.824031292373576	26.650647259190862	29.97109963174751	21.554221816688056
24	23.62036185442212	24.834395437013555	27.450551288156667	24.09469142040766
25	23.62014373843531	28.36620220079031	25.516298717114474	22.497355343659915
26	21.43614836249423	24.861223703391342	26.984728245656765	26.717899688457663
27	22.61041213015708	25.39924313752577	27.500209027820695	24.49013570449646
28	19.052431447962977	26.658935666689693	28.531243297477488	25.757389587869845
29	22.18130527877041	27.665904472468313	26.040067906777224	24.112722341984057
30	22.53109061628672	26.736803073981307	27.024716176572163	23.70739013315981
31	23.93001385036516	27.449824234867293	24.65452245322321	23.965639461544335
32	24.999436533700738	27.45091481480135	23.611928036265418	23.937720615232493
33	23.243166607896526	26.42576967678846	24.428190764242068	25.902872951072947
34	21.131513034247845	28.27030387192229	27.449751529538357	23.148431564291506
35	19.87683717278058	27.841996779153927	25.422872369430316	26.858293678635174
36	22.58671019292359	30.206592192174725	25.065816499020293	22.14088111588139
37	20.40547761948212	27.96763158755721	25.744884271292662	25.882006521668004
38	21.19403961713374	29.853971346829866	24.454873619961976	24.497115416074422
39	21.576760468658552	26.803837387261296	25.66592628406699	25.95347586001316
40	22.74055466895446	25.64069753492582	27.57771290846763	24.041034887652092
41	19.46539771632562	26.798966130222514	26.970478001185093	26.76515815226677
42	20.46356917730285	24.58145359764144	29.14771178153503	25.807265443520684
43	21.16132221911205	24.533395375214027	28.12743790056092	26.177844505113
44	22.084898012599833	24.356067077936476	27.639367027406276	25.919667882057418
45	21.261873689032036	23.77493338374236	25.458861507254177	29.504331419971425
46	23.272830382102857	25.433123820810444	26.044575637171324	25.249470159915372
47	18.863252182068685	25.89531159686349	31.47682699403453	23.764609227033297
48	20.28202397094695	27.512278112424248	26.93390722072974	25.271790695899053
49	21.96747890636644	23.93677544595631	29.776612876840808	24.31913277083644
50	20.671288302076103	24.658230424999	28.3803070346041	26.290174238320795
51	20.282387497591635	24.062555665017467	26.04821090361818	29.606845933772718
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	486.0
1	961.0
2	1436.0
3	3814.0
4	6192.0
5	4215.0
6	2238.0
7	2201.0
8	2164.0
9	2181.5
10	2199.0
11	2206.0
12	2213.0
13	2263.0
14	2313.0
15	2417.0
16	2521.0
17	2386.5
18	2252.0
19	2338.0
20	2424.0
21	2690.0
22	2956.0
23	3509.5
24	4063.0
25	4833.5
26	7031.0
27	8458.0
28	9479.5
29	10501.0
30	12570.5
31	14640.0
32	17159.0
33	19678.0
34	24186.0
35	28694.0
36	32099.5
37	35505.0
38	38493.0
39	41481.0
40	47852.5
41	54224.0
42	63083.0
43	71942.0
44	112583.0
45	153224.0
46	140610.5
47	127997.0
48	132581.0
49	137165.0
50	124019.0
51	110873.0
52	102546.0
53	94219.0
54	86977.0
55	79735.0
56	80313.5
57	80892.0
58	75969.0
59	71046.0
60	65155.5
61	59265.0
62	52676.5
63	46088.0
64	39922.5
65	33757.0
66	27748.5
67	21740.0
68	17881.5
69	14023.0
70	11381.0
71	8739.0
72	6905.0
73	5071.0
74	4383.0
75	2842.0
76	1989.0
77	1463.0
78	937.0
79	607.5
80	278.0
81	252.0
82	226.0
83	174.0
84	122.0
85	86.0
86	50.0
87	43.0
88	36.0
89	28.0
90	20.0
91	27.0
92	34.0
93	19.5
94	5.0
95	3.5
96	2.0
97	2.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1375415.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.41560065216576
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.41400566178103	19.852734608470595
2	11.074188546228276	6.072110614603651
3	4.183743287786071	3.4409950562736586
4	2.062300526145499	2.2615683059822542
5	1.2666404487868357	1.7362854356909954
6	0.8690403926064707	1.429515861258021
7	0.6565956487550458	1.2600674867352624
8	0.5044757843486055	1.106440531391157
9	0.4130843203112064	1.0192459285191022
>10	5.726351604111126	39.48906070242664
>50	0.7560263702445966	12.528879215679103
>100	0.06422074473600703	3.359262308649363
>500	0.004796570139067306	0.8466463151413334
>1k	0.0034641895448819433	1.9518379154052121
>5k	2.664761188370726E-4	0.4466645586999838
>10k+	7.994283565112177E-4	3.1986851550736164
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGC	15683	1.1402376737202953	No Hit
GAATCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTC	14282	1.03837750787944	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCG	13819	1.0047149405815698	No Hit
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	6114	0.4445203811213343	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3423	0.24887034095164004	No Hit
GAACTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCT	2909	0.21149980187797868	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCT	2701	0.19637709345906507	No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	2579	0.1875070433287408	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	2436	0.1771101812907377	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACACAACTT	2183	0.15871573306965533	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACACAACTTCGTA	2098	0.15253578011000316	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTC	1990	0.14468360458479806	No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT	1920	0.13959423155920214	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.9082131574833777E-4	0.0	0.0	0.1458468898477914	0.0
2	2.9082131574833777E-4	0.0	0.0	0.9534576836809254	0.0
3	2.9082131574833777E-4	0.0	0.0	1.294954613698411	0.0
4	2.9082131574833777E-4	0.0	0.0	1.8972455586132186	0.0
5	2.9082131574833777E-4	0.0	0.0	4.0043914018677995	0.0
6	2.9082131574833777E-4	0.0	0.0	4.798987941821196	0.0
7	2.9082131574833777E-4	0.0	0.0	5.673560343605384	0.0
8	2.9082131574833777E-4	0.0	0.0	6.81081709883926	0.0
9	2.9082131574833777E-4	0.0	0.0	7.223856072530836	0.0
10	2.9082131574833777E-4	0.0	0.0	9.127281584103708	0.0
11	2.9082131574833777E-4	0.0	0.0	10.338479658866596	0.0
12	3.635266446854222E-4	0.0	0.0	12.491793385996226	0.0
13	3.635266446854222E-4	0.0	0.0	12.9448202906032	0.0
14	4.3623197362250665E-4	0.0	0.0	13.167444007808552	0.0
15	4.3623197362250665E-4	0.0	0.0	13.623815357546631	0.0
16	4.3623197362250665E-4	0.0	0.0	14.238320797722869	0.0
17	5.089373025595911E-4	0.0	0.0	14.92756731604643	0.0
18	5.089373025595911E-4	0.0	0.0	15.690173511267508	0.0
19	5.089373025595911E-4	0.0	0.0	16.348520264792807	0.0
20	5.089373025595911E-4	0.0	0.0	16.88741216287448	0.0
21	5.089373025595911E-4	0.0	0.0	17.506498038773753	0.0
22	5.816426314966755E-4	0.0	0.0	18.171897209205948	0.0
23	5.816426314966755E-4	0.0	0.0	18.781458687014464	0.0
24	5.816426314966755E-4	0.0	0.0	19.273019415958093	0.0
25	5.816426314966755E-4	0.0	0.0	19.717612502408365	0.0
26	6.5434796043376E-4	0.0	0.0	20.149045924321022	0.0
27	6.5434796043376E-4	0.0	0.0	20.69629893523046	0.0
28	6.5434796043376E-4	0.0	0.0	21.142273422930533	0.0
29	6.5434796043376E-4	0.0	0.0	21.649683913582447	0.0
30	6.5434796043376E-4	0.0	0.0	22.30694008717369	0.0
31	6.5434796043376E-4	0.0	0.0	22.79123028322361	0.0
32	7.270532893708444E-4	0.0	0.0	23.256689799078824	0.0
33	7.270532893708444E-4	0.0	0.0	23.729710669143493	0.0
34	7.270532893708444E-4	0.0	0.0	24.23603057986135	0.0
35	7.270532893708444E-4	0.0	0.0	24.785028518665275	0.0
36	7.270532893708444E-4	0.0	0.0	25.254923059585654	0.0
37	7.997586183079289E-4	0.0	0.0	25.749900938989324	0.0
38	7.997586183079289E-4	0.0	0.0	26.308423275884007	0.0
39	8.724639472450133E-4	0.0	0.0	27.247558009764326	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTAGA	25	3.891206E-5	45.000004	42
ATCGTAC	20	7.0337724E-4	45.0	21
TATCGTG	20	7.0337724E-4	45.0	1
ATTAGCG	140	0.0	40.17857	1
TACGGCT	1935	0.0	40.000004	7
CACGGGA	355	0.0	39.929577	4
CGTTTTT	2010	0.0	39.850746	1
CCGGTAT	85	0.0	39.705883	42
CCGTAAG	40	3.458881E-7	39.375	1
ATGCGTA	40	3.458881E-7	39.375	16
AGTTGCG	75	0.0	39.0	1
AGCGACT	730	0.0	38.219177	19
AACGAGC	740	0.0	38.006756	15
CGATGAA	705	0.0	37.659573	19
CGACGGT	120	0.0	37.500004	28
CACGCGT	30	1.1400385E-4	37.500004	43
TATAGCG	30	1.1400385E-4	37.500004	1
CGGTCTA	120	0.0	37.500004	31
ACCGTAG	30	1.1400385E-4	37.500004	41
GTACGCT	30	1.1400385E-4	37.500004	38
>>END_MODULE