SRR2935787.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935787.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1446979
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5797	0.4006277907281308	No Hit
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	3722	0.2572255713455413	RNA PCR Primer, Index 16 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	3388	0.23414299723769316	Illumina PCR Primer Index 7 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT	2645	0.18279463627322856	RNA PCR Primer, Index 16 (96% over 26bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2544	0.1758145764382206	No Hit
CGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG	2438	0.16848896908662808	RNA PCR Primer, Index 16 (95% over 24bp)
CGTTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTT	1915	0.13234469885188382	RNA PCR Primer, Index 16 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC	1759	0.12156361633444578	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1639	0.11327047593641648	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACATA	25	3.891308E-5	45.0	30
CGCAATA	25	3.891308E-5	45.0	10
CGAATAT	355	0.0	42.464787	14
GCTACGA	370	0.0	41.351353	10
CAATGCG	55	6.184564E-11	40.909092	1
CACGACG	165	0.0	40.90909	26
CTACGAA	375	0.0	40.8	11
GCGATAT	105	0.0	40.714287	9
TACGAAT	380	0.0	39.67105	12
CGTTAGG	200	0.0	39.375	2
CGTTTTT	3020	0.0	39.33775	1
CACAACG	395	0.0	39.3038	12
TCGTTAG	35	6.2494128E-6	38.571426	1
CGACGGT	190	0.0	37.894737	28
CGGTCTA	180	0.0	37.5	31
ACAACGA	445	0.0	36.91011	13
GACCTAT	370	0.0	36.486485	33
CTGACTA	405	0.0	36.11111	22
TTTCGCG	25	0.0021072882	36.0	1
CGCAACC	25	0.0021072882	36.0	12