##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935787.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1446979
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.098528036688855	33.0	31.0	34.0	30.0	34.0
2	32.12349384476209	33.0	31.0	34.0	30.0	34.0
3	32.156485339455514	34.0	31.0	34.0	30.0	34.0
4	35.81194267504919	37.0	35.0	37.0	35.0	37.0
5	34.891538163304375	37.0	35.0	37.0	33.0	37.0
6	35.373735900797456	37.0	35.0	37.0	33.0	37.0
7	35.94067363797263	37.0	35.0	37.0	35.0	37.0
8	35.929003116147506	37.0	35.0	37.0	35.0	37.0
9	37.78098369084831	39.0	38.0	39.0	35.0	39.0
10	37.436664941232735	39.0	37.0	39.0	35.0	39.0
11	37.35064710683431	39.0	37.0	39.0	35.0	39.0
12	37.223746163558694	39.0	37.0	39.0	34.0	39.0
13	37.15723448647147	39.0	37.0	39.0	34.0	39.0
14	38.181372362694965	40.0	38.0	41.0	33.0	41.0
15	38.327441517810556	40.0	38.0	41.0	34.0	41.0
16	38.33689362457921	40.0	38.0	41.0	34.0	41.0
17	38.348796354335484	40.0	37.0	41.0	34.0	41.0
18	38.32978709435313	40.0	37.0	41.0	34.0	41.0
19	38.229695109604215	40.0	37.0	41.0	34.0	41.0
20	38.24487224762764	40.0	37.0	41.0	34.0	41.0
21	38.146846637027906	40.0	37.0	41.0	34.0	41.0
22	38.20033255492996	40.0	37.0	41.0	34.0	41.0
23	38.20796639066635	40.0	37.0	41.0	34.0	41.0
24	38.16606046114007	40.0	37.0	41.0	34.0	41.0
25	37.9852776025084	40.0	36.0	41.0	34.0	41.0
26	38.00181827103227	40.0	36.0	41.0	34.0	41.0
27	38.00516455318287	40.0	36.0	41.0	34.0	41.0
28	37.88112543443962	40.0	36.0	41.0	34.0	41.0
29	37.81185145050481	40.0	36.0	41.0	33.0	41.0
30	37.681486047827924	40.0	36.0	41.0	33.0	41.0
31	37.59890572012448	40.0	36.0	41.0	33.0	41.0
32	37.538096959250964	40.0	35.0	41.0	33.0	41.0
33	37.399895921088	40.0	35.0	41.0	33.0	41.0
34	37.27253125304514	40.0	35.0	41.0	32.0	41.0
35	37.21167273332923	40.0	35.0	41.0	32.0	41.0
36	37.12760931568461	40.0	35.0	41.0	32.0	41.0
37	37.078866382995194	39.0	35.0	41.0	32.0	41.0
38	36.996011690563584	39.0	35.0	41.0	32.0	41.0
39	36.92410187017227	39.0	35.0	41.0	32.0	41.0
40	36.77167118527636	39.0	35.0	41.0	31.0	41.0
41	36.682859944753865	39.0	35.0	41.0	31.0	41.0
42	36.69509854669626	39.0	35.0	41.0	31.0	41.0
43	36.612602532586855	39.0	35.0	41.0	31.0	41.0
44	36.42938356396326	39.0	35.0	41.0	31.0	41.0
45	36.37966065851681	39.0	35.0	41.0	31.0	41.0
46	36.239495528269586	38.0	35.0	41.0	30.0	41.0
47	36.155587607007426	38.0	35.0	40.0	30.0	41.0
48	36.09271316307977	38.0	35.0	40.0	30.0	41.0
49	36.0610084873381	38.0	35.0	40.0	30.0	41.0
50	35.943041329556266	38.0	35.0	40.0	30.0	41.0
51	34.76735391460415	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	16.0
9	27.0
10	49.0
11	34.0
12	31.0
13	26.0
14	48.0
15	61.0
16	118.0
17	227.0
18	429.0
19	668.0
20	1081.0
21	1652.0
22	2600.0
23	3719.0
24	5819.0
25	8109.0
26	11066.0
27	13518.0
28	15141.0
29	17224.0
30	20977.0
31	26894.0
32	35599.0
33	51069.0
34	93543.0
35	110551.0
36	106915.0
37	163158.0
38	282313.0
39	473936.0
40	359.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.737855214208366	21.301829535881307	24.12377788482072	12.836537365089612
2	32.74981876032756	23.791706721382962	25.338861172138643	18.119613346150842
3	32.547604353622276	22.87441628385761	27.050427131285247	17.527552231234868
4	28.301309141321333	25.324417285945405	26.96162141952302	19.412652153210242
5	24.86580662193439	30.269893343303533	25.24114033444853	19.62315970031355
6	26.02629340163195	33.3720116186897	25.369200244094763	15.232494735583584
7	79.77855932947196	5.932912640750143	9.18085196813499	5.107676061642913
8	80.72874589057616	5.0752637045872815	9.045120903620578	5.150869501215982
9	74.53342446573171	7.113994052436144	11.517997151306274	6.8345843305258756
10	41.836543584944906	24.22039297045776	18.59522494797782	15.34783849661951
11	30.273901694495915	24.828349271136624	24.415558207824716	20.482190826542748
12	27.322234807830657	21.08973247020171	30.220134500915357	21.367898221052275
13	25.2753495385904	22.337297224078583	31.48331800254185	20.90403523478917
14	21.318554035684002	24.730144666923294	31.815527384986236	22.13577391240647
15	20.265325205134282	24.558960427207303	33.09895997108458	22.07675439657383
16	23.767518395222044	24.346794252024388	29.59794164255321	22.287745710200355
17	24.16289386369809	24.79828663719377	27.54925952622671	23.48955997288143
18	25.007964870257275	24.121428161707943	29.217286498283666	21.653320469751115
19	24.052733315410933	26.318764819669116	27.236055257194476	22.392446607725475
20	24.72268084056507	26.874612554847026	27.268398504746788	21.134308099841117
21	24.617150629000147	26.28130746887135	28.720872935958297	20.380668966170205
22	23.70649470379321	23.46101774801155	29.13089961913753	23.701587929057713
23	22.479662800911417	25.072236708342	29.010372645352835	23.43772784539375
24	22.90883281650943	24.5049859051168	28.981968639489587	23.604212638884185
25	23.286308923626397	25.70168606455242	27.074131690922947	23.93787332089823
26	21.83936325268024	26.042810572924697	26.727754860298596	25.390071314096474
27	22.214766074697696	25.155790097852144	28.43109678855049	24.198347038899666
28	20.52759577022196	25.612189257757024	28.868283506533267	24.991931465487752
29	21.939088265966543	26.10549289243313	27.853686888337702	24.101731953262625
30	24.288949597748136	24.39800439398222	28.013191621993133	23.29985438627651
31	24.170426799559635	25.968586966362334	26.717181106291104	23.143805127786926
32	23.670903309585004	25.79719539813639	26.196233670288233	24.335667621990368
33	24.005324196135536	24.10297592432233	26.001413980437864	25.89028589910427
34	21.07121112331278	25.093384216356974	28.389631086560346	25.445773573769902
35	21.418279048970305	26.31897214817907	26.292019441885472	25.970729360965155
36	23.55583598656235	26.454772322196796	25.246047109184033	24.74334458205682
37	21.57197858434711	26.56458732296737	26.92672112034798	24.93671297233754
38	21.354767415422064	25.92345846069639	26.125465538891717	26.59630858498983
39	21.628786596073613	24.43518530676672	26.100102351174414	27.835925745985257
40	23.23233440153589	24.078234722134876	27.385193565352367	25.30423731097687
41	20.76954814133446	25.032360524928144	26.331550077782744	27.866541255954647
42	21.367760002045642	23.828818524664143	27.805033798002597	26.998387675287617
43	22.233840297613163	23.252376157497796	28.134202362301043	26.379581182587998
44	22.45989748296278	23.141178966660885	27.420093864527406	26.978829685848932
45	22.245934460693626	23.341458307273292	26.438946245937224	27.97366098609586
46	22.515254195119624	23.76019278787045	27.11103616569418	26.613516851315744
47	20.673762369737226	24.76352455702536	29.01299880647888	25.54971426675854
48	20.300363723315957	24.57250588985742	29.094271582379566	26.032858804447056
49	22.52769390571667	22.859557740644473	28.82308589136401	25.789662462274848
50	20.861532890249272	23.019269802809855	28.992196845980487	27.12700046096039
51	20.64563480188724	22.518571451278838	27.50544410112379	29.33034964571013
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	506.0
1	1279.0
2	2052.0
3	5902.0
4	9752.0
5	6412.5
6	3073.0
7	3019.0
8	2965.0
9	3012.0
10	3059.0
11	3026.0
12	2993.0
13	2965.0
14	2937.0
15	3047.0
16	3157.0
17	3030.5
18	2904.0
19	2963.5
20	3023.0
21	3464.0
22	3905.0
23	4364.5
24	4824.0
25	5530.0
26	7585.5
27	8935.0
28	10948.5
29	12962.0
30	15255.0
31	17548.0
32	21051.0
33	24554.0
34	29397.0
35	34240.0
36	36573.5
37	38907.0
38	41800.5
39	44694.0
40	49405.5
41	54117.0
42	58065.0
43	62013.0
44	68731.0
45	75449.0
46	83177.0
47	90905.0
48	108282.5
49	125660.0
50	135899.5
51	146139.0
52	145313.5
53	144488.0
54	126411.0
55	108334.0
56	100083.0
57	91832.0
58	85509.5
59	79187.0
60	71571.0
61	63955.0
62	58319.0
63	52683.0
64	45641.0
65	38599.0
66	33876.0
67	29153.0
68	23939.0
69	18725.0
70	15948.0
71	13171.0
72	10717.0
73	8263.0
74	6960.0
75	4453.5
76	3250.0
77	2209.5
78	1169.0
79	833.5
80	498.0
81	385.0
82	272.0
83	183.0
84	94.0
85	96.5
86	99.0
87	58.5
88	18.0
89	12.0
90	6.0
91	7.0
92	8.0
93	7.0
94	6.0
95	3.5
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1446979.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.449798472929743
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.79438337870776	19.707413552337847
2	10.918016906554872	5.99394727617942
3	4.04490831326205	3.3309575412156427
4	2.02933923279326	2.2281981189353934
5	1.28488424206297	1.7634906752835804
6	0.8634754471010411	1.422133620554789
7	0.6619199181366373	1.271869785064819
8	0.5293953311437587	1.1625436121924866
9	0.4457604700660954	1.1012431563461476
>10	6.784747235861429	46.30891084997343
>50	0.571626793196394	9.455297291376526
>100	0.06405269794682192	3.2651391322965635
>500	0.003357601074854971	0.5978703327732955
>1k	0.0038741550863711205	1.9799979430443013
>5k	2.582770057580747E-4	0.4109871124258026
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5797	0.4006277907281308	No Hit
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	3722	0.2572255713455413	RNA PCR Primer, Index 16 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	3388	0.23414299723769316	Illumina PCR Primer Index 7 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT	2645	0.18279463627322856	RNA PCR Primer, Index 16 (96% over 26bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2544	0.1758145764382206	No Hit
CGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG	2438	0.16848896908662808	RNA PCR Primer, Index 16 (95% over 24bp)
CGTTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTT	1915	0.13234469885188382	RNA PCR Primer, Index 16 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC	1759	0.12156361633444578	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1639	0.11327047593641648	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.146570199014637E-4	0.0	0.0	0.19447414233378646	0.0
2	4.146570199014637E-4	0.0	0.0	0.876723159078328	0.0
3	4.146570199014637E-4	0.0	0.0	1.3401714883215305	0.0
4	4.146570199014637E-4	0.0	0.0	1.8466059286278516	0.0
5	4.146570199014637E-4	0.0	0.0	3.089540345782489	0.0
6	4.146570199014637E-4	0.0	0.0	4.169445444612534	0.0
7	4.146570199014637E-4	0.0	0.0	5.0178337073309285	0.0
8	4.146570199014637E-4	0.0	0.0	6.197187381434008	0.0
9	4.146570199014637E-4	0.0	0.0	6.747921013366469	0.0
10	4.837665232183743E-4	0.0	0.0	7.7672861872909005	0.0
11	4.837665232183743E-4	0.0	0.0	9.139317156641527	0.0
12	4.837665232183743E-4	0.0	0.0	10.235877645770948	0.0
13	4.837665232183743E-4	0.0	0.0	10.71003794802827	0.0
14	4.837665232183743E-4	0.0	0.0	10.93803019947076	0.0
15	4.837665232183743E-4	0.0	0.0	11.267336982775838	0.0
16	5.528760265352849E-4	0.0	0.0	11.893330863820415	0.0
17	5.528760265352849E-4	0.0	0.0	12.603983886428207	0.0
18	5.528760265352849E-4	0.0	0.0	13.424313690799936	0.0
19	6.219855298521955E-4	0.0	0.0	13.920105267595453	0.0
20	6.219855298521955E-4	0.0	0.0	14.4152057493578	0.0
21	6.910950331691061E-4	0.0	0.0	14.998144409835941	0.0
22	6.910950331691061E-4	0.0	0.0	15.64037902415999	0.0
23	6.910950331691061E-4	0.0	0.0	16.250132171925095	0.0
24	6.910950331691061E-4	0.0	0.0	16.752972918058934	0.0
25	6.910950331691061E-4	0.0	0.0	17.214071524189364	0.0
26	6.910950331691061E-4	0.0	0.0	17.645522153396836	0.0
27	7.602045364860167E-4	0.0	0.0	18.129634224131795	0.0
28	7.602045364860167E-4	0.0	0.0	18.579882638241468	0.0
29	7.602045364860167E-4	0.0	0.0	19.079544347222733	0.0
30	7.602045364860167E-4	0.0	0.0	19.7075424038635	0.0
31	8.293140398029274E-4	0.0	0.0	20.1808042825777	0.0
32	9.675330464367486E-4	0.0	0.0	20.66353416324632	0.0
33	9.675330464367486E-4	0.0	0.0	21.152898556233367	0.0
34	9.675330464367486E-4	0.0	0.0	21.630928990676438	0.0
35	9.675330464367486E-4	0.0	0.0	22.176202971846863	0.0
36	0.0010366425497536592	0.0	0.0	22.646493141918437	0.0
37	0.0010366425497536592	0.0	0.0	23.1216209772222	0.0
38	0.0011057520530705699	0.0	0.0	23.663163045213512	0.0
39	0.0011057520530705699	0.0	0.0	24.401114321631482	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACATA	25	3.891308E-5	45.0	30
CGCAATA	25	3.891308E-5	45.0	10
CGAATAT	355	0.0	42.464787	14
GCTACGA	370	0.0	41.351353	10
CAATGCG	55	6.184564E-11	40.909092	1
CACGACG	165	0.0	40.90909	26
CTACGAA	375	0.0	40.8	11
GCGATAT	105	0.0	40.714287	9
TACGAAT	380	0.0	39.67105	12
CGTTAGG	200	0.0	39.375	2
CGTTTTT	3020	0.0	39.33775	1
CACAACG	395	0.0	39.3038	12
TCGTTAG	35	6.2494128E-6	38.571426	1
CGACGGT	190	0.0	37.894737	28
CGGTCTA	180	0.0	37.5	31
ACAACGA	445	0.0	36.91011	13
GACCTAT	370	0.0	36.486485	33
CTGACTA	405	0.0	36.11111	22
TTTCGCG	25	0.0021072882	36.0	1
CGCAACC	25	0.0021072882	36.0	12
>>END_MODULE